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Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

Virologica Sinica 2024年第3期39卷 369-377页

作者： Shengyao Chen Muhammad Saqib Hafiz Sajid Khan Yuan Bai Usman Ali Ashfaq Muhammad Khalid Mansoor Abulimti Moming Jing Liu Min Zhou Saifullah Khan Niazi Qiaoli Wu Awais-Ur-Rahman Sial Shuang Tang Muhammad Hassan Sarfraz Aneela Javed Sumreen Hayat Mohsin Khurshid Iahtasham Khan Muhammad Ammar Athar Zeeshan Taj Bo Zhang Fei Deng Ali Zohaib Shu Shen Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Faculty of Veterinary Science University of Agriculture FaisalabadFaisalabad 38040Pakistan The Children's Hospital Faisalabad 38000Pakistan Department of Bioinformatics and biotechnology Government College University FaisalabadFaisalabad 38000Pakistan f Department of Microbiology Faculty of Veterinary and Animal SciencesThe Islamia University of BahawalpurBahawalpur 63100Pakistan Hubei Jiangxia Laboratory Wuhan 430200China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China Armed Forces Institute of Pathology(AFIP) Rawalpindi 46000Pakistan Department of Clinical Studies Faculty of Veterinary&Animal SciencesPir Mehr Ali Shah Arid Agriculture UniversityRawalpindi 46300Pakistan Institute of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan l Atta-ur-Rahman School of Applied Biosciences National University of Science and TechnologyIslamabad 44000Pakistan Department of Clinical Sciences University of Veterinary and Animal SciencesLahore Sub-campusJhang 35200Pakistan Department of Molecular Pathology National Medical Center DHA Phase 1Karachi 75500Pakistan

Infectious diseases caused by arboviruses are a public health concern in ***,studies on data prevalence and threats posed by arboviruses are *** study investigated the seroprevalence of arboviruses in a healthy population in Pakistan,including severe fever with thrombocytopenia syndrome virus(SFTSV),Crimean-Congo hemorrhagic fever virus(CCHFV),Tamdy virus(TAMV),and Karshi virus(KSIV)based on a newly established luciferase immunoprecipitation system(LIPS)assays,and Zika virus(ZIKV)by enzyme-linked immunosorbent assays(ELISA).Neutralizing activities against these arboviruses were further examined from the antibody positive *** results showed that the seroprevalence of SFTSV,CCHFV,TAMV,KSIV,and ZIKV was 17.37%,7.58%,4.41%,1.10%,and 6.48%,respectively,and neutralizing to SFTSV(1.79%),CCHFV(2.62%),and ZIKV(0.69%)were identified,as well as to the SFTSV-related Guertu virus(GTV,0.83%).Risk factors associated with the incidence of exposure and levels of antibody response were ***,co-exposure to different arboviruses was demonstrated,as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing ***,individuals aged40 years,and outdoor workers had a high risk of exposure to *** these results reveal the substantial risks of infection with arboviruses in Pakistan,and indicate the threat from co-exposure to multiple *** findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

关键词： Arbovirus Pakistan Seroprevalence Tick-borne virus Mosquito-borne virus

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Allelopathic Effect of Three Wild Plants (Azadirachta indica, Tithonia diversifoliaand Thevetia peruviana) on Tomato (Lycopersicum esculentumMill.) Growth and Stimulation of Metabolites Involved in Plant Resistance

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American Journal of Plant Sciences 2021年第3期12卷 285-299页

作者： G. Y. Fangue-Yapseu R. A. Mouafo-Tchinda M. Fomekong Kenne P. Effa Onomo P. F. Djocgoue Laboratory of Phytoprotection and Plant Valorization Biotechnology Centre University of Yaoundé I Yaoundé Cameroon Laboratory of Plant Physiology Department of Biological Sciences Higher Teacher’s Training College Yaoundé Cameroon Department of Biochemistry Faculty of Science University of Yaoundé I Yaoundé Cameroon Centre SEVE Département de Biologie Université de Sherbrooke Sherbrooke Canada Saint-Jean-sur-Richelieu Research and Development Centre Agriculture and Agri-Food Canada Saint-Jean-sur-Richelieu Canada Laboratoire de Biotechnologie Végétale et Environnement University of Yaoundé I Yaoundé Cameroon Department of Plant Physiology Faculty of Science University of Yaoundé I Yaoundé Cameroon

The aim of this study was to determine the allelopathic effects of Azadirachta indica oil and aqueous extracts of Tithonia diversifolia and Thevetia peruviana on the growth and stimulation of metabolites involved in tomato plant resistance. Randomized in blocks within a shaded area, the different treatments prepared at 10% and 15% (v/v and w/v) in water were subsequently applied on tomatoes seeds to monitor the effect on germination, and on tomatoes leaves to monitor the effect on growth and resilience of the plants. The result showed that in stressful conditions all the treatments significantly inhibit (p T. diversifolia at 10% promotes a better aerial and root growth in tomato plants, T. peruviana at 15% induces the activation of resistance mechanisms in tomato plants by increasing protein levels to 104.5%, phenol levels to 183.33% and peroxidase enzyme activity to 586.15%. Therefore, allelopathic compound of wild plants would be a good alternative for growth promotion

关键词： Allelopathic Wild Plants Growth Plant Resistance Tomato

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The inhibitory role of Co2+ on α-glucosidase: inhibition kinetics and molecular dynamics simulation integrating study

The inhibitory role of Co2+ on α-glucosidase: inhibition ki...

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中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Xuan Li Zhi-Rong Lü Dong Shen Yi Zhan Jun-Mo Yang Yong-Doo Park Hai-Meng Zhou Qing Sheng Jinhyuk Lee College of Life Sciences Zhejiang Sci-Tech University Zhejiang Provincial Key Laboratory of Applied Enzymology Yangtze Delta Region Institute of Tsinghua University Zhejiang Provincial Key Laboratory of Applied Enzymology Yangtze Delta Region Institute of Tsinghua University School of Life Science Tsinghua University Department of Dermatology Sungkyunkwan University School of Medicine Samsung Medical CenterSeoul 135-710 Korea Korean Bioinformation Center (KOBIC) Korea Research Institute of Bioscience and BiotechnologyDaejeon 305-806 Korea Department of Bioinformatics University of Sciences and Technology Daejeon 305-350 Korea

α-glucosidase(EC 3.***.1.20) is a target enzyme to treat type 2 diabetes mellitus, hence, dueto the clinical relevance, it is important for studying this enzyme’s inhibition. In this study,we investigated Co2+-induced inhibition as well as structural changes of α-glucosidase integratingwith computational simulations. α-glucosidase activity was inhibited by Co2+ ina dose-dependent manner. Co2+ inhibited α-glucosidase in a parabolic non-competitiveinhibition reaction(Ki = 0.78 ± 0.08 mM) and directly induced the regional unfolding ofthe enzyme resulting in a slight decrease of hydrophobic surface. The computational simulationsusing molecular dynamics showed that the simulation with Co2+ ions lost the secondarystructure by positioning Co2+ ions near the active site for glucose degradation, implyingthat the Co2+ ions can stimulate the unfolding of the enzyme. Our study revealedthe mechanism of Co2+ ligand binding mediated structural change as well as inhibition ofα-glucosidase, and suggested that Co2+ could act as a potent inhibitor of α-glucosidasefor the treatment of type 2 diabetes *** with computational simulations. α-glucosidase activity was inhibited by Co2+in a dose-dependent manner. Co2+ inhibited α-glucosidase in a parabolic noncompetitiveinhibition reaction(Ki = 0.78 ± 0.08 mM) and directly induced the regional unfoldingof the enzyme resulting in a slight decrease of hydrophobic surface. The computationalsimulations using molecular dynamics showed that the simulation with Co2+ ionslost the secondary structure by positioning Co2+ ions near the active site for glucose degradation,implying that the Co2+ ions can stimulate the unfolding of the enzyme. Ourstudy revealed the mechanism of Co2+ ligand binding mediated structural change as wellas inhibition of α-glucosidase, and suggested that Co2+ could act as a potent inhibitor ofα-glucosidase for the treatment of type 2 diabetes mellitus.

关键词： α-glucosidase Co2+ Inhibition kinetics Unfolding Molecular dynamics

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Optimization of biogenic synthesis of biocompatible platinum nanoparticles with catalytic,enzyme mimetic and antioxidant activities

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Food Bioscience 2022年第6期50卷 1268-1283页

作者： Priyadarshani S.Sadalage Mudasir A.Dar Renuka D.Bhor Bharat M.Bhalerao Pravin N.Kamble Ana C.Paiva-Santos Mansingraj S.Nimbalkar Kailas D.Sonawane Kalpana Pai Pramod S.Patil Kiran D.Pawar School of Nanoscience and biotechnology Shivaji UniversityKolhapur416004MaharashtraIndia School of the Environment and Safety Engineering Jiangsu UniversityZhenjiang212013PR China Department of Zoology Centre for Advanced StudySavitribai Phule Pune UniversityPune411007India Department of Biochemistry Post Graduate InstituteMahatma Phule Krishi VidyapeethRahuriMaharashtraIndia Department of Pharmaceutical Technology Faculty of PharmacyUniversity of CoimbraCoimbraPortugal REQUIMTE/LAVQ Group of Pharmaceutical TechnologyCoimbraPortugal Department of Botany Shivaji UniversityKolhapur416004MaharashtraIndia Department of Biochemistry Shivaji UniversityKolhapur416004MaharashtraIndia Department of Physics Shivaji UniversityKolhapur416004MaharashtraIndia

Owing to platinum's nobility,catalytic potential and vast range of possible biomedical applications,synthesis of platinum nanoparticles is attracting the attention of scientists *** the plant mediated methods are simple and easy,they require fine tuning of reaction parameters so as to synthesize nanoparticles of desired *** present study aimed to optimize the food waste almond skin extract mediated synthesis of biocompatible platinum nanoparticles with catalytic,enzyme-mimicking,and antioxidant *** optimization of reaction parameters indicated that 10 mM of precursor in 2:1 ratio with extract,pH 9,and incubation at 90°C for 14 min were the optimum *** characterization using various spectroscopic and imaging techniques indicated that platinum nanoparticles were 6–8 nm in size,face centered cubic,irregularly shaped,and negatively *** tested for catalytic activity,platinum nanoparticles catalyzed highest of 90.39%reduction of 4-nitrophenol in 16 *** reaction containing 30 ppm of 4-nitrophenol,catalyst dosage of 0.6 mg/mL,and pH 10 followed pseudo-first order ***,platinum nanoparticles were found to possess amylase-mimicking activity of 3.83μg/mL/*** addition,platinum nanoparticles showed strong antioxidant activities in a dose-dependent manner signifying them as potentially good antioxidant *** tested for cytotoxicity,peripheral blood mononuclear cells were found unaffected when treated with relatively high concentrations(100μg/mL)of PtNP demonstrating good ***,the present study successfully demonstrated the use of almond skin extract as food waste for the biogenic synthesis of biocompatible platinum nanoparticles with biological activities.

关键词： Bioreducing agent Platinum nanoparticles Catalytic reduction α-Amylase enzyme mimic Antioxidant activity

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Inactivation of Protein Tyrosine Phosphatase 1B （PTP1B） Activity by the Aqueous Partition of Guava Leaf Extract

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Journal of Pharmacy and Pharmacology 2018年第10期6卷 890-906页

作者： Wan-Jung Wu Wei-Li Yan Shiou-Cherng Yu Gerry Gunawan Chien-Yih Lin Chih-Yan Huang Chia-Ting Chang Haw-Wen Chen Chong-Kuei Lii Alice L. Yu Ching-Chu Chen Yu-Ting Chung Jeng-Dau Tsai Henry J. Tsai Department of biotechnology Asia University 500 Lioufeng Rd. Wufeng District Taichung 413 Taiwan Department of Nutrition China Medical University 91 Hsueh-Shih Rd Taichung 404 Taiwan Genomics Research Center Academia Sinica 128 Section 2 of Academia Rd Nankang District Taipei 115 Taiwan Division of Endocrinology and Metabolism Department of Medicine China Medical University Hospital Taichung Taiwan School of Chinese Medicine China Medical University Taichung Taiwan Asia University Hospital Wufeng Taichung College of Medicine Chung Shan Medical University 110 Section 1 of Jianguo North Rd Taichung 402 Taiwan Department of Pediatrics Chung Shan Medical University Hospital 110 Section 1 of Jianguo North Rd Taichung 402 Taiwan Department of Health and Nutrition Asia University 500 Lioufeng Rd. Wufeng District Taichung 413 Taiwan

Guava leaf tea has been used as a folk medicine for treating hyperglycemic conditions in Asia and Africa. The hypoglycemic efficacy of guava leaf has been documented by many scientists in these regions, but the hypoglycemic mechanism is poorly understood. Guava leaves were extracted with methanol and the crude extract was partitioned against hexane, ethyl acetate, and butanol in sequence. The leftover in water is defined as the aqueous partition. A second smaller batch was extracted with hot water directly. Oral glucose tolerance test was carried out on healthy mice instead of diabetic mice that lack endogenous insulin. Glucose uptake was examined with 3T3-L1 adipocytes. Oxidative effect on PTP1B （protein tyrosine phosphatase 1b） was carried out with real-time PTP1B enzymatic assay. The aqueous partition of guava leaf extract possesses a potent inhibitory effect on PTP1B enzymatic activity and this PTP1B inhibition is through a slow oxidative but reversible inactivation on the enzyme. The reversible inactivation would suggest guava leaf extract may augment PTP1B inhibition alongside the endogenous H2O2 which itself is induced by insulin. In addition, our study confirmed the hypoglycemic efficacy being associated with guava leaf and found the most effective molecules reside in the aqueous partition which is also less cytotoxic to Chinese hamster ovary cells when compared to other less polar partitions. The guava leaf extract can modulate insulin activity through a redox regulation on PP1B enzymatic activity. It is speculated that a compound similar to gallocatechin in the aqueous partition can reduce an oxygen molecule to hydrogen peroxide which in turn oxidizes the catalytic residue Cys in PTP1B. Therefore, the guava leaf tea can serve as a functional hypoglycemic drink that is suitable for either healthy or diabetic subjects.

关键词： Guava leaf extract hypoglycemic oxidative protein tyrosine phosphatase 1b slow inactivation.

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HIV prevalence in suspected Ebola cases during the 2014-2016 Ebola epidemic in Sierra Leone

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Infectious Diseases of Poverty 2019年第2期8卷 87-90页

作者： William J.Liu Hai-Yang Hu Qiu-Dong Su Zhe Zhang Yang Liu Yu-Lan Sun Xian-Da Yang Da-Peng Sun Shao-Jian Cai Xiu-Xu Yang Idrissa Kamara Abdul Kamara Matt Lebby Brima Kargbo Patricia Ongpin Xiao-Ping Dong Yue-Long Shu Wen-Bo Xu Gui-Zhen Wu Michael Gboun George F.Gao NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention(China CDC)Beijing 102206China Jiangsu Provincial Center for Disease control and Prevention Nanjing 210009China Beijing Institute of biotechnology Beijing 100071China. Beijing Center for Disease Prevention and Control Beijing 100013China Jilin Provincial Center for Disease control and Prevention Changchun 130021China Shandong Provincial Center for Disease control and Prevention Jinan 250014China Fujian Provincial Center for Disease control and Prevention Fuzhou 350001China The Ministry of Health and Sanitation FreetownSierra Leone UNAIDS FreetownSierra Leone Chinese Center for Disease Control and Prevention Beijing 102206China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101China Sierra Leone-China Friendship Biological Safety Laboratory FreetownSierra Leone

Background:The 2014-2016 Ebola virus epidemic in West Africa was the largest outbreak of Ebola virus disease(EVD)in *** the influence of other prevalent diseases such as human immunodeficiency virus infection and acquired immune deficiency syndrome(HIV/AIDS)will help improve treatment and supportive care of patients with *** presentation:We examined HIV and hepatitis C virus(HCV)antibody prevalence among suspected EVD cases from the Sierra Leone-China Friendship Biological Safety Laboratory during the epidemic in Sierra *** and HCV antibodies were tested in 678 EVD-negative samples by enzyme-linked immunosorbent assay.A high HIV prevalence(17.6%)and low HCV prevalence(0.22%)were observed among the suspected ***,we found decreased HIV positive rates among the suspected cases over the course of the *** suggests a potentially beneficial effect of an improved public health system after assistance from the World Health Organization and other international aid ***:This EVD epidemic had a considerable impact on the public health system and influenced the prevalence of HIV found among suspected cases in Sierra Leone,but also provided an opportunity to establish a better surveillance network for infectious diseases.

关键词： HIV HCV Ebola Prevalence Sierra Leone

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Cellulosomal hemicellulases:Indispensable players for ensuring effective lignocellulose bioconversion

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Green Carbon 2024年第1期2卷 57-69页

作者： Min Xiao Ya-Jun Liu Edward A.Bayer Akihiko Kosugi Qiu Cui Yingang Feng CAS Key Laboratory of Biofuels Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Shandong Provincial Key Laboratory of Synthetic Biology Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Shandong Engineering Laboratory of Single Cell Oil Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China Shandong Energy Institute Qingdao 266101China Qingdao New Energy Shandong Laboratory Qingdao 266101China University of Chinese Academy of Sciences Beijing 100049China Department of Biomolecular Sciences The Weizmann Institute of ScienceRehovot 7610001Israel Department of Life Sciences and the National Institute for biotechnology in the Negev Ben-Gurion University of the NegevBeer-Sheva 8499000Israel Biological Resources and Post-harvest Division Japan International Research Center for Agricultural Sciences(JIRCAS)1-1 OhwashiTsukubaIbaraki 305-8686Japan

The bioconversion of lignocellulose has attracted global attention,due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil *** cellulosome system is a multi-enzyme complex produced by anaerobic bacteria,which comprises cellulases,hemicellulases,and associated enzymatic and non-enzymatic components that promote biomass *** enhance their efficiency in degrading recalcitrant lignocellulosic matrices,cellulosomes have been employed to construct biocatalysts for lignocellulose bioconversion,such as consolidated bioprocessing and consolidated ***,the second most abundant polysaccharides in plant cell walls,hold valuable application potential but can also induce inhibitory effects on cellulose hydrolysis,thus highlighting the indispensable roles of hemicellulases within the cellulosome *** review evaluated current research on cellulosomal hemicellulases,comparing their types,abundance,and regulation,primarily focusing on eight known cellulosome-producing species of different *** also reviewed their growth conditions,their hemicellulose-degrading capabilities,and the inhibitory effects of hemicellulose on cellulosome-based lignocellulose ***,we proposed strategies for targeted enhancement of hemicellulase in cellulosomes to improve lignocellulose bioconversion in future studies.

关键词： Cellulosome Lignocellulose Hemicellulase Saccharification Biocatalyst

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Protein engineering for natural product biosynthesis:expanding diversity for therapeutic applications

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Journal of Bio-X Research 2023年第2期6卷 49-60页

作者： Sarah Oluwatobi Otun Jordy Alexis Lerma-Escalera Khayalethu Ntushelo Ikechukwu Achilonu Protein Structure-Function and Research unit School of Molecular and Cell BiologyFaculty of ScienceUniversity of the WitwatersrandBraamfontein 2050JohannesburgSouth Africa Facultad de Ciencias Químicas Universidad Autónoma de Nuevo LeónSan Nicolás de los GarzaMexico Centro de Investigación en Biotecnología y Nanotecnología Facultad de Ciencias QuímicasParque de Investigación e Innovación TecnológicaUniversidad Autónoma de Nuevo LeónApodacaMexico Department of Agriculture and Animal Health University of South AfricaFlorida 1710South Africa

In this dispensation of the fourth industrial revolution,protein engineering has become a popular approach for increasing enzymatic activity,stability,and titer in the biosynthesis of natural *** is attributed to its numerous advantages(over direct isolation from plants or via chemical synthesis),including decreasing or eliminating reaction byproducts,high precision,moderate handling of intricate and chemically unstable chemicals,overall reusability,and cost ***,protein engineering tools have advanced to redesign and enhance natural product *** methods include direct evolution,substrate engineering,medium engineering,enzyme engineering and immobilization,structure-assisted protein engineering,and advanced *** successes in implementing these emerging protein engineering technologies were critically discussed in this ***,the advantages,limitations,and applications in industrial and medical biotechnology were ***,future research directions and potential were also highlighted.

关键词： antibodies engineering industrial enzymes natural product biosynthesis protein engineering polyketides synthetic biology

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肝胆肿瘤中肿瘤特异性CircRNA衍生抗原肽的鉴定

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Engineering 2023年第3期22卷 159-170页

作者： Wenwen Wang Lili Ma Zheng Xing Tinggan Yuan Jinxia Bao Yanjing Zhu Xiaofang Zhao Yan Zhao Yali Zong Yani Zhang Siyun Shen Xinyao Qiu Shuai Yang 王红阳高栋王鹏陈磊 Fudan University Shanghai Cancer Center Shanghai 200032China Bio-Med Big Data Center CAS Key Laboratory of Computational BiologyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China State Key Laboratory of Pharmaceutical biotechnology&Model Animal Research Center of Nanjing University and MOE Key Laboratory of Model Animal for Disease Study Nanjing UniversityNanjing 210061China School of Medicine Nanjing UniversityNanjing 210093China The International Cooperation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghai 200438China National Center for Liver Cancer Shanghai 200441China Institute of Metabolism and Integrative Biology Fudan UniversityShanghai 200433China State Key Laboratory of Cell Biology&Shanghai Key Laboratory of Molecular Andrology Center for Excellence in Molecular Cell ScienceChinese Academy of SciencesShanghai 200031China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Faculty of Health Sciences University of MacaoMacao 999078China Key Laboratory of Signaling Regulation and Targeting Therapy of Liver Cancer(SMMU) Ministry of Education&Shanghai Key Laboratory of Hepatobiliary Tumor Biology(EHBH)Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghai 200438China

基于肿瘤抗原的免疫治疗的应用受到验证免疫原性肽稀缺性的阻碍。本研究旨在研究环状RNA(circRNA)在肝胆肿瘤类器官中作为肿瘤抗原肽新来源的潜力。使用RNA测序(RNA-seq)和基于算法的评分工具,预测3950个翻译的肿瘤特异性环状RNA在27个... 详细信息

基于肿瘤抗原的免疫治疗的应用受到验证免疫原性肽稀缺性的阻碍。本研究旨在研究环状RNA(circRNA)在肝胆肿瘤类器官中作为肿瘤抗原肽新来源的潜力。使用RNA测序(RNA-seq)和基于算法的评分工具,预测3950个翻译的肿瘤特异性环状RNA在27个类器官中产生18971个抗原肽。从抗原格局来看,11个氨基酸长度(mer)肽和人白细胞抗原(HLA)-A结合肽具有最高的免疫原性相关评分。在分析的3/5类器官中,有13个预测抗原肽通过质谱(MS)免疫肽组学被直接确认为HLA-A、HLA-B和HLA-C(HLA-ABC)结合肽。在流式细胞术和酶联免疫吸附试验(ELISA)中,由HLA-ABC分子呈递的circRNA衍生的肿瘤特异性肽刺激CD8(CD8)T细胞,显示CD107a干扰素γ(IFNγ)共表达和IFNγ分泌增加。免疫原性环状RNA衍生肽诱导的靶向类器官的细胞毒性T细胞活性在杀伤实验中得到验证。值得注意的是,来自circTBC1D15的抗原肽YGFNEILKK不仅被认为是类器官的HLA-ABC呈递肽,而且还显著降低了肿瘤类器官的存活率。本研究的发现强调了产生肿瘤抗原的一个关键亚群,这对靶向肿瘤特异性circRNA具有重要意义。

关键词： Tumor antigen Patient-derived hepatobiliary tumor organoid Circular RNA Mass-spectrometry-based immunopeptidomics

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创制人工熊胆——在医药需求和动物福利之间实现平衡的创新路径（英文）

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Engineering 2024年第7期 100-112页

作者：李勇黄宇虹冯楠张和平屈晶马双刚刘云宝李江徐少锋王玲张咪蔡杰王伟平冯茹于航于博梁待亮秦和平罗素芗李艳芬李美凤王瑞华马辰王琰岑小波徐孝先张伯礼王晓良庾石山 State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine Zhongshan Belling biotechnology Co. Ltd. Zhongshan Anshi Biopharmaceutical Co. Ltd. WestChina-Frontier Pharma Tech Co. Ltd. Tianjin University of Traditional Chinese Medicine

Bear bile has been a valuable and effective medicinal material in traditional Chinese medicine (TCM) for over 13 ***,the current practice of obtaining it through bear farming is under scrutiny for its adverse impact on bear ***,we present a new approach for creating artificial bear bile (ABB)as a high-quality and sustainable alternative to natural bear *** study addresses the scientific challenges of creating bear bile alternatives through interdisciplinary collaborations across various fields,including resources,chemistry,biology,medicine,pharmacology,and TCM.A comprehensive efficacy assessment system that bridges the gap between TCM and modern medical terminology has been established,allowing for the systematic screening of therapeutic *** the utilization of chemical synthesis and enzyme engineering technologies,our research has achieved the environmentally friendly,large-scale production of bear bile therapeutic compounds,as well as the optimization and recomposition of ABB *** resulting ABB not only closely resembles natural bear bile in its composition but also offers advantages such as consistent product quality,availability of raw materials,and independence from threatened or wild *** preclinical efficacy evaluations have demonstrated the equivalence of the therapeutic effects from ABB and those from commercially available drained bear bile (DBB).Furthermore,preclinical toxicological assessment and phase I clinical trials show that the safety of ABB is on par with that of the currently used *** innovative strategy can serve as a new research paradigm for developing alternatives for other endangered TCMs,thereby strengthening the integrity and sustainability of TCM.

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