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Journal of environmental sciences 2016年第3期28卷 211-217页

作者： Sha Chen Yuanjing Sun Jingbo Chao Liping Cheng Yun Chen Jingfu Liu Key Laboratory of Beijing on Regional Air Pollution Control Beijing University of Technology Chemical metrology and Analytical Science division National Institute of Metrology State Key Laboratory of environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of Sciences

Using the ionic liquid（IL）1-octyl-3-methylimidazolium hexafluorophosphate as the extractant and methanol as the dispersion solvent,a dispersive liquid–liquid microextraction method was developed to extract silver nanoparticles（AgN Ps）from environmental water *** that influenced the extraction efficiency such as IL concentration,pH and extraction time were *** the optimized conditions,the highest extraction efficiency for AgN Ps was above 90% with an enrichment factor of 〉*** extracted AgN Ps in the IL phase were identified by transmission electron microscopy and ultraviolet–visible spectroscopy,and quantified by inductively coupled plasma mass spectrometry after microwave digestion,with a detection limit of 0.01 μg/*** spiked recovery of AgN Ps was 84.4% with a relative standard deviation（RSD）of 3.8%（n = 6）at a spiked level of 5 μg/L,and 89.7% with a RSD of 2.2%（n = 6）at a spiked level of 300 μg/L,*** existed environmental ions had a very limited influence on the extraction *** developed method was successfully applied to the analysis of Ag NPs in river water,lake water,and the influent and effluent of a wastewater treatment plant,with recoveries in the range of 71.0%–90.9% at spiking levels of 0.11–4.7 μg/L.

关键词： Silver nanoparticle 1-Octyl-3-methylimidazolium hexafluorophosphate Ionic liquid Dispersive liquid–liquid microextraction

Biodegradation of Crystal Violet by Agrobacterium radiobacter

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Journal of environmental sciences 2011年第8期23卷 1384-1393页

作者： G.K.Parshetti S.G.Parshetti A.A.Telke D.C.Kalyani R.A.Doong S.P.Govindwar Department of biomedical Engineering and environmental sciences National Tsinghua University division of Applied Life sciences Gyeongsang National University division of Chemical Engineering Konkuk University Department of Biochemistry Shivaji University

Agrobacterium radiobacter MTCC 8161 completely decolorized the Crystal Violet with 8 hr （10 mg/L） at static anoxic conditions. The decreased decolorization capability by A. radiobacter was observed, when the Crystal Violet concentration was increased from 10 to 100 mg/L. Semi-synthetic medium containing 1% yeast extract and 0.1% NH4C1 has shown 100% decolorization of Crystal Violet within 5 hr. A complete degradation of Crystal Violet by A. radiobacter was observed up to 7 cycles of repeated addition （10 rag/L）. When the effect of increasing inoculum concentration on decolorization of Crystal Violet （100 mg/L） was studied, maximum decolorization was observed with 15% inoculum concentration. A significant increase in the activities of laccase （184%） and aminopyrine N-demethylase （300%） in cells obtained after decolorization indicated the involvement of these enzymes in decolorization process. The intermediates formed during the degradation of Crystal Violet were analyzed by gas chromatography and mass spectroscopy （GC/MS）. It was detected the presence of N,N,N＇,N＂-tetramethylpararosarfiline, IN, N-dimethylaminophenyl] [N-methylaminophenyl] benzophenone, N, N-dimethylaminobenzaldehyde, 4-methyl amino phenol and phenol. We proposed the hypothetical metabolic pathway of Crystal Violet biodegradation by A. radiobacter. Phytotoxicity and microbial toxicity study showed that Crystal Violet biodegradation metabolites were less toxic to bacteria （A. radiobacter, P. aurugenosa and A. vinelandii） contributing to soil fertility and for four kinds of plants （Sorghum bicolor, Vigna radiata, Lens culinaris and Triticum aestivum） which are most sensitive, fast growing and commonly used in Indian agriculture.

关键词： Agrobacterium radiobacter Crystal Violet biodegradation gas chromatography and mass spectroscopy toxicity study

Source apportionment of PM10 in Delhi, India using PCA/APCS, UNMIX and PMF

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Particuology 2018年第2期16卷 107-118页

作者： Srishti Jain S.K. Sharma T.K. Mandal Mohit Saxena environmental sciences and biomedical metrology division CSIR-National Physical Laboratory Dr. K. S. Krishnan Road New Delhi 110 012 India Academy of Scientific and Innovative Research (AcSIR) CSIR-National Physical Laboratory campus New Delhi 110 012 India

Source apportionment of particulate matter （PM10） measurements taken in Delhi, India between January 2013 and June 2014 was carried out using two receptor models, principal component analysis with absolute principal component scores （PCA/APCS） and UNMIX. The results were compared with previous estimates generated using the positive matrix factorization （PMF） receptor model to investigate each model＇s source-apportioning capability. All models used the PM10 chemical composition （organic carbon （OC）, elemental carbon （EC）, water soluble inorganic ions （WSIC）, and trace elements） for source apportionment. The average PM10 concentration during the study period was 249.7±103.9 μg/m3 （range： 61.4-584.8 μg/m3）. The UNMIX model resolved five sources （soil dust （SD）, vehicular emissions （VE）, secondary aerosols （SA）, a mixed source of biomass burning （BB） and sea salt （SS）, and industrial emissions （IE））. The PCA/APCS model also resolved five sources, two of which also included mixed sources （SD, VE, SD＋SS, （SA＋BB＋SS） and 1E）. The PMF analysis differentiated seven individual sources （SD, VE, SA, BB, SS, IE, and fossil fuel combustion （FFC））. All models identified the main sources contributing to PM10 emissions and reconfirmed that VE, SA, BB, and SD were the dominant contributors in Delhi.

关键词： Receptor model PCA/APCS UNMIX PMF Source apportionment

Development of a Seaweed Species-Selection Index for Successful Culture in a Seaweed-Based Integrated Aquaculture System

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Journal of Ocean University of China 2013年第1期12卷 125-133页

作者： Yun Hee Kang Jae Ran Hwang Ik Kyo Chung Sang Rul Park Marine and environmental Research Institute Jeju National University Marine environmental Research division National Fisheries Research and Development Institute division of Earth environmental System Pusan National University School of Marine biomedical sciences Jeju National University

Integrated multi-trophic aquaculture (IMTA) has been proposed as a concept that combines the cultivation of fed aquaculture species (e.g., finfish/shrimp) with extractive aquaculture species (e.g., shellfish/seaweed). In seaweed-based integrated aquaculture, seaweeds have the capacity to reduce the environmental impact of nitrogen-rich effluents on coastal ecosystems. Thus, selection of optimal species for such aquaculture is of great importance. The present study aimed to develop a seaweed species-selection index for selecting suitable species in seaweed-based integrated aquaculture system. The index was synthesized using available literature-based information, reference data, and physiological seaweed experiments to identify and prioritize the desired species. Undaria pinnatifida, Porphyra yezoensis and Ulva compressa scored the highest according to a seaweed-based integrated aquaculture suitability index (SASI). Seaweed species with the highest scores were adjudged to fit the integrated aquaculture systems. Despite the application of this model limited by local aquaculture environment, it is considered to be a useful tool for selecting seaweed species in IMTA.

关键词： seaweed IMTA species-selection model water temperature physiological characteristics uptake rate

Coating zirconium oxide-nanocomposite with humic acid for recovery of mercury and chromium in hazardous waste of chemical oxygen demand test

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Journal of environmental sciences 2023年第4期126卷 40-47页

作者： Yujian Lai Liteng Hao Lijie Dong Sujuan Yu Jingfu Liu State Key Laboratory of environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China China Testing&Certification International Group Co. Ltd.Beijing 100024China School of Environment Hangzhou Institute for Advanced StudyUCASHangzhou 310024China division of Chemical metrology and Analytical Chemistry National Institute of MetrologyBeijing 100029China Institute of Environment and Health Jianghan UniversityWuhan 430056China

Hazardous waste of chemical oxygen demand (COD) test (HWCOD) is one of the most common laboratory wastewaters,containing large amounts of H_(2)SO_(4)and highly toxic Cr^(3+)and Hg^(2+).Current treatment methods suffered from incomplete removal of Cr^(3+)and ***,a humic acid-coated zirconium oxide-resin nanocomposite (HA-HZO-201) was fabricated for efficient recovery of Cr^(3+)and Hg^(2+)in *** synthesized HA-HZO-201 shows excellent tolerance to wide p H range (1–5) and high salinity (3.5 mol/L Na Cl),as well as adsorption capacity for Cr^(3+)(37.5 mg/g) and Hg^(2+)(121.3 mg/g).After treating with HA-HZO-201 by using a fixed-bed adsorption procedure,the final Cr^(3+)and Hg^(2+)concentrations in HWCOD decreased to 0.28 and 0.02 mg/L,*** addition,the HA-HZO-201 can be regenerated by desorption and recovery of Cr^(3+)and Hg^(2+)using HNO_(3)and thiourea as eluents,*** 5 cycles of adsorption/desorption,the removal efficiencies still reach up to86.0%for Cr^(3+)and 89.7%for Hg^(2+),indicating an excellent regeneration of *** hope this work open new opportunities for treatment of HWCODwith high-efficiency and low-cost.

关键词： COD analysis wastewater Nanocomposite ZrO_(2) Resin Humic acid

Genotoxicity of Pesticide Waste Contaminated Soil and Its Leachate

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biomedical and environmental sciences 2004年第3期17卷 257-265页

作者： S.D.SIVANESAN K.KRISHNAMURTHI S.D.WACHASUNDER T.CHAKRABARTI environmental Biotechnology division Environmental Biotechnology DivisionInstrumentation Division National Environmental Engineering Research Institute Nehru Marg Nagpur-440 020 IndiaEnvironmental Biotechnology Division

Objective Improper land disposal of hazardous waste can result in leaching of hazardous constituents which may contaminate ground and surface water leading to adverse impact on human health and environment consequences. The present study utilized mammalian cell culture for the genotoxicity assessment of waste and its leachate. Methods Genotoxic potential and chemical analysis of pesticide derived tarry waste contaminated soil extract and its leachate was assessed using in vitro human lymphocyte cultures and GC-MS. Results The investigation revealed that the soil extract could cause significant to highly significant genotoxicity in the form of DNA strand break at 25 mL (P<0.01), 50 mL, 100 mL and 200 mL (P<0.001) and chromosomal aberration at 25 mL (P<0.01) and 50 mL and 100 mL (P<0.001). The leachate could cause significant DNA strand break and chromosomal aberration only at 100 mL and 200 mL (P<0.01) dose levels. Conclusion The genotoxicity observed is attributed to carbaril and tetra methyl naphthyl carbamate, the major ingredients of the extracts, as revealed by GC-MS.

关键词： Genotoxicity Soil extract Leachate DNA strand break Chromosomal aberration Gas chromatography mass spectrometery (GC-MS) Fluorimetric analysis of DNA unwinding assay (FADU)

Genotoxic Effects of PAH Containing Sludge Extracts in Chinese Hamster Ovary Cell Cultures

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biomedical and environmental sciences 2003年第1期16卷 68-82页

作者： K.KRISHNAMURTHI FONTDEVI T.CHAKRABARTI environmental Biotechnology division National Environmental Engineering Research InstituteNehru MargNagpur-440020IndiaEnvironmental Biotechnology DivisionNational Environmental Engineering Research InstituteNehru MargNagpur-440020IndiaEnvironmental Biotechnology DivisionNational Environmental Engineering Research InstituteNehru MargNagpur-440020India

Objective Many studies have been conducted in order to evaluate the genotoxicity of chemicals and waste materials, which utilized in vivo test protocols. The use of animals for routine toxicity testing is now questioned by a growing segment of society. Methods Keeping the above fact in mind, we have conducted in the present study the genotoxicity evaluation of oily sludge samples generated from a petroleum refinery and petrochemical industry and ETP sludge from petroleum refinery using DNA damage, chromosomal aberration, p53 protein induction and apoptosis in short term in vitro mammalian Chinese Hamster Ovary cell cultures. Results It is evident from the results that the oily sludge compounds derived from petroleum refinery and petrochemical industry could cause DNA damage, chromosomal aberration, p53 protein accumulation and apoptotic cell death on exposure to oily sludge extracts in the presence of metabolic activation system (S-9 mix), however, ETP sludge extract could not cause significant genotoxicity in comparison to oily sludge extract and negative control. Conclusion The effect may be attributed to polycyclic aromatic hydrocarbons present in the samples as evidenced from GC-MS.

关键词： DNA damage Chromosomal aberration p53 protein induction Apoptosis Genotoxicity Polycyclic aromatic hydrocarbons (PAHs) Gas chromatograph mass spectrometer (GC-MS)

Basophile:Accurate Fragment Charge State Prediction Improves Peptide Identification Rates

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Genomics, Proteomics & Bioinformatics 2013年第2期11卷 86-95页

作者： Dong Wang Surendra Dasari Matthew C.Chambers Jerry D.Holman Kan Chen Daniel C.Liebler Daniel J.Orton Samuel O.Purvine Matthew E.Monroe Chang Y.Chung Kristie L.Rose David L.Tabb Department of biomedical Informatics Vanderbilt University Medical Center division of biomedical Statistics and Informatics Mayo Clinic Department of Biochemistry Vanderbilt University Medical Center Biological sciences division and environmental Molecular sciences Laboratory Pacific Northwest National Laboratory Department of Pharmacology Vanderbilt University Medical Center Vanderbilt-Ingram Cancer Center Vanderbilt University School of Medicine

In shotgun proteomics, database search algorithms rely on fragmentation models to pre- dict fragment ions that should be observed for a given peptide sequence. The most widely used strat- egy （Naive model） is oversimplified, cleaving all peptide bonds with equal probability to produce fragments of all charges below that of the precursor ion. More accurate models, based on fragmen- tation simulation, are too computationally intensive for on-the-fly use in database search algorithms. We have created an ordinal-regression-based model called Basophile that takes fragment size and basic residue distribution into account when determining the charge retention during CID/higher- energy collision induced dissociation （HCD） of charged peptides. This model improves the accuracy of predictions by reducing the number of unnecessary fragments that are routinely predicted for highly-charged precursors. Basophile increased the identification rates by 26% （on average） over the Naive model, when analyzing triply-charged precursors from ion trap data. Basophile achieves simplicity and speed by solving the prediction problem with an ordinal regression equation, which can be incorporated into any database search software for shotgun proteomic identification.

关键词： Fragmentation Basicity Fragment size Ordinal regression

Studies on Rhizospheric Mycoflora of Tea (Camellia sinensis): In vitro Antagonism with Dominant Bacteria

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应用与环境生物学报 2007年第3期13卷 357-360页

作者： SINGH Shuchi SOOD Anchal SHARMA Shivesh KUMAR Vivek SINGH Kamal Deep PANDEY Piyush Department of Microbiology Division of Life Sciences SBS (PG) Institute of Biomedical Sciences and Research Department of Soil and Water Public Authority of Agriculture Affairs & Fish Resources

Species of Trichoderma, Penicillium, Aspergillus and Mucor were found to dominate the rhizosphere of tea bushes in different regions of the Indian Himalayas. The Himachal Himalayas were dominated by Monilia humiscola (45.08%) and Mucor hiemalis (33.11%). In the Uttaranchal Himalayas, Aspergillus clavatum dominated the rhizosphere (54.01%), followed by A. flavous (20.07%). Although the fungal isolates require a mesophillic temperature (15 ℃ to 35 ℃), the tea rhizospheric isolates tolerate a wide range of temperatures (5 ℃ to 45 ℃). When tested for pH tolerance, these fungi showed a wide range (2.0 to 13.0) of pH tolerance under in vitro conditions. Seasonal variations also influenced the colony forming units (cfu) of the rhizospheric fungi, with the maximum forming in November and March, and the minimum in December^February. During this period, the populations of the fungi were found inversely proportional to the bacterial populations. In addition, the dominant bacteria in the tea rhizosphere, i.e., Bacillus subtilis and B. mycoides, showed antagonistic activity against fungal isolates by inhibiting the growth and causing structural abnormalities in mycelium. Tab 5, Ref

关键词：细菌茶拮抗作用真菌区系丛矮病优势菌种

Bioengineering breakthroughs:The impact of stem cell models on advanced therapy medicinal product development

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World Journal of Stem Cells 2024年第10期16卷 860-872页

作者： José Mauro Granjeiro Priscila Grion de Miranda Borchio Icaro Paschoal Brito Ribeiro Katiucia Batista Silva Paiva division of Biological metrology The National Institute of MetrologyQualityand TechnologyDuque de Caxias 25250020Rio de JaneiroBrazil Laboratory of Regenerative Medicine Arthur SáEarp Neto UniversityPetropolis 25680120Rio de JaneiroBrazil Laboratory of Extracellular Matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508000São PauloBrazil

The burgeoning field of bioengineering has witnessed significant strides due to the advent of stem cell models,particularly in their application in advanced therapy medicinal products(ATMPs).In this review,we examine the multifaceted impact of these developments,emphasizing the potential of stem cell models to enhance the sophistication of ATMPs and to offer alternatives to animal *** cell-derived tissues are particularly promising because they can reshape the preclinical landscape by providing more physiologically relevant and ethically sound platforms for drug screening and disease *** also discuss the critical challenges of reproducibility and accuracy in measurements to ensure the integrity and utility of stem cell models in research and ***,this review highlights the imperative of stem cell models to align with regulatory standards,ensuring using stem cells in ATMPs translates into safe and effective clinical *** regulatory approval serving as a gateway to clinical adoption,the collaborative efforts between scientists and regulators are vital for the progression of stem cell applications from bench to *** advocate for a balanced approach that nurtures innovation within the framework of rigorous validation and regulatory compliance,ensuring that stem cell-base solutions are maximized to promote public trust and patient health in ATMPs.

关键词： Stem cells Advanced therapy medicinal products Tissue-engineered products Health Three-dimensional cell culture