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Biochemical Characterization of Detoxifying Enzymes in Dimethoate-Resistant Strains of Melon Aphid, <i>Aphis gossypii</i>(Hemiptera: Aphididae)

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Advances in entomology 2016年第3期4卷 167-182页

作者： Dayanandam Lokeshwari Nallur Kothandaraman Krishna Kumar Hanumanthappa Manjunatha Seshadri Shivashankar Division of entomology and nematology Indian Institute of Horticultural Research Hessaraghatta Lake Post Bengaluru India Division of Horticultural Science Indian Council of Agriculture Research Krishi Anusandhan Bhawan-II New Delhi India Department of Biotechnology and Bioinformatics Kuvempu University Jnanasahyadri Shankaraghatta Shimoga India Division of Plant Physiology and Biochemistry Indian Institute of Horticultural Research Hessaraghatta Lake Post Bengaluru India

The melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is a highly polyphagous sap sucking pest on wide varieties of crops including cotton and vegetables. It is a notorious vector of many plant viruses that are persistently and non-persistently transmitted. In nature, aphids are regulated by their natural enemies. However, chemical control remains a major management tool even though resistance to insecticides has been documented worldwide. A better understanding of mechanisms by which insecticide resistance occurs and its early detection is desirable to develop effective management strategies. The present investigation was conducted to study the development of resistance to an organophosphate (OP) compound-dimethoate, identify biochemical mechanism(s) involved in resistance and study cross-resistance to imidacloprid in laboratory selected A. gossypii strains in comparison to susceptible strains. Bioassay studies revealed that the LC50 values increased dramatically with dimethoate selection in resistant strains and the resistance ratio (RR) was 270-, 243- and 210-fold greater than that of the susceptible strains by 30th generation. Further, biochemical assays revealed enhanced activities of carboxylesterases (CarE), glutathione S-transferases (GSTs) and cytochrome P450-mediated p-Nitroanisole O-demethylase (PNOD) in resistant strains supporting their role in dimethoate detoxification. This study thus revealed that enhanced activity of detoxifying enzymes viz., CarE, GSTs and PNODs is one of the mechanisms underlying dimethoate resistance in A. gossypii collected from South India. Interestingly, the possibility of negatively correlated cross-resistance to imidacloprid was identified in three OP- resistant strains exhibiting 2.97-, 2.56- and 3.76-fold sensitivity to imidacloprid (a novel neonicotinoid). This indicated that the latter was less affected by the resistance mechanism(s) present.

关键词： Aphis gossypii Carboxylesterases Dimethoate Glutathione S-Transferases LC50 Melon Aphid p-Nitroanisole O-Demethylase Resistance

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Nanobody-based bispecific T-cell engager (Nb-BiTE): a new platform for enhanced T-cell immunotherapy

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Signal Transduction and Targeted Therapy 2023年第10期8卷 4444-4447页

作者： Xiao-mei Yang Xuan-dong Lin Wei Shi Shen-xia Xie Xia-ning Huang Shi-hua Yin Xiao-bing Jiang Bruce D.Hammock Zhi Ping Xu Xiao-ling Lu Guangxi Key Laboratory of Nanobody Research Guangxi Nanobody Engineering Research CenterCollege of StomatologyHospital of StomatologyLaboratory Animal CenterSchool of Basic Medical SciencePharmaceutical CollegeGuangxi Medical UniversityNanning CityGuangxi 530021P.R.China Department of Neurosurgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan CityHubei 430022P.R.China Department of entomology and nematology UCD Comprehensive Cancer CenterUniversity of CaliforniaDavisCA 95616USA Australian Institute for Bioengineering and Nanotechnology The University of QueenslandBrisbaneQLD 4072Australia Institute of Biomedical Health Technology and Engineering and Institute of Systems and Physical Biology Shenzhen Bay LaboratoryShenzhen 518107P.R.China

A novel bispecific T-cell engager(BiTE)has been developed as an efficient immunotherapeutic molecule specifically bringing the T-cell and the tumor target together for enhanced *** general BiTE construct consists of two single-chain variable antibody fragments(scFvs)targeting a tumor-associated antigen(TAA)and a T-cell marker in *** binding of BiTEs to tumor antigens induces immediate T-cells’cytotoxicity against tumor cells without involving any typical costimulatory signals,specific TCR and MHC recognition.

关键词： immunotherapy tumor cytotoxicity

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A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence antiquenching

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Journal of Pharmaceutical Analysis 2022年第4期12卷 637-644页

作者： Lingyuan Xu Xiuyuan Zhang A.M.Abd El-Aty Yuanshang Wang Zhen Cao Huiyan Jia J.-Pablo Salvador Ahmet Hacimuftuoglu Xueyan Cui Yudan Zhang Kun Wang Yongxin She Fen Jin Lufei Zheng Baima Pujia Jing Wang Maojun Jin Bruce D.Hammock Institute of Quality Standard and Testing Technology for Agro-Products Chinese Academy of Agricultural SciencesBeijing100081China Department of Pharmacology Faculty of Veterinary MedicineCairo UniversityGiza12211Egypt Department of Medical Pharmacology Medical FacultyAtaturk UniversityErzurum25240Türkiye Institute of Livestock and Poultry Ningbo Academy of Agricultural SciencesNingboZhejiang315040China Nanobiotechnology for Diagnostics Group Instituto de Química Avanzada de CatalunaIQAC-CSICBarcelona08034Spain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid28029Spain Inspection and Testing Center of Agricultural and Livestock Products of Tibet Department of Agriculture and Rural Affairs of Tibet Autonomous RegionLhasa850000China Department of entomology&nematology and the UC Davis Comprehensive Cancer Center University of CaliforniaDavisCA95616USA

Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and *** nanoparticles were functionalized with monoclonal antibodies against the tested *** oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal *** detection signals were generated by DNA-RNA hybridization and ribonuclease H dissociation of the *** resulting fluorescence signal enables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concentration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,*** mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%-17.6%,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).The proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

关键词： Organophosphate pesticides Fluorescence anti-quenching Gold nanoparticles DNA-RNA hybridization DNA oligonucleotides Ribonuclease H

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Assessment of UAV Based Vegetation Indices for Nitrogen Concentration Estimation in Spring Wheat

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Advances in Remote Sensing 2018年第2期7卷 71-90页

作者： Olga S. Walsh Sanaz Shafian Juliet M. Marshall Chad Jackson Jordan R. McClintick-Chess Steven M. Blanscet Kristin Swoboda Craig Thompson Kelli M. Belmont Willow L. Walsh Department of Plant Sciences Southwest Research and Extension Center University of Idaho Moscow ID USA Department of entomology Plant Pathology and Nematology Idaho Falls Research and Extension Center University of Idaho Idaho Falls ID USA BASF-Chemical Co. Caldwell ID USA Take Flight UAS LLC Boise ID USA Seminis Payette ID USA Vallivue High School Caldwell ID USA

Unmanned Aerial Vehicles (UAVs) have become increasingly popular in recent years for agricultural research. High spatial and temporal resolution images obtained with UAVs are ideal for many applications in agriculture. The objective of this study was to evaluate the performance of vegetation indices (VIs) derived from UAV images for quantification of plant nitrogen (N) concentration of spring wheat, a major cereal crop worldwide. This study was conducted at three locations in Idaho, United States. A quadcopter UAV equipped with a red edge multispectral sensor was used to collect images during the 2016 growing season. Flight missions were successfully carried out at Feekes 5 and Feekes 10 growth stages of spring wheat. Plant samples were collected on the same days as UAV image data acquisition and were transferred to lab for N concentration analysis. Different VIs including Normalized Difference Vegetative Index (NDVI), Red Edge Normalized Difference Vegetation Index (NDVIred edge), Enhanced Vegetation Index 2 (EVI2), Red Edge Simple Ratio (SRred edge), Green Chlorophyll Index (CIgreen), Red Edge Chlorophyll Index (CIred edge), Medium Resolution Imaging Spectrometer (MERIS) Terrestrial Chlorophyll Index (MTCI) and Red Edge Triangular Vegetation Index (core only) (RTVIcore) were calculated for each flight event. At Feekes 5 growth stage, red edge and green based VIs showed higher correlation with plant N concentration compare to the red based VIs. At Feekes 10 growth stage, all calculated VIs showed high correlation with plant N concentration. Empirical relationships between VIs and plant N concentration were cross validated using test data sets for each growth stage. At Feekes 5, the plant N concentration estimated based on NDVIred edge showed one to one correlation with measured N concentration. At Feekes 10, the estimated and measured N concentration were highly correlated for all empirical models, but the model based on CIgreen was the only model that had a one to

关键词： Unmanned Aerial Vehicles and Systems (UAV) Vegetation Indices (VIs) Plant Nitrogen Concentration

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Population Dynamic of Olive Pit Scale,Pollinia pollini Costa(Hemiptera:Asterolecanidae)on Two Olive Cultivars in North Region of Jordan

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Journal of Agricultural Science and Technology(A) 2019年第1期9卷 1-7页

作者： Hail Kamel Shannag Nawaf Mohammed Freihat Mazin Ahmed Alkelani John Lowell Capinera Department of Plant Production Jordan University of Science and TechnologyIrbid 22110Jordan Department of entomology and nematology University of FloridaGainesvilleFlorida 32611United States of America

Olive tree,Olea europaea L.,is considered the most important tree in *** olive pit scale,Pollinia pollini Costa(Hemiptera:Asterolecanidae)has become a destructive threat to some olive groves in Jordan.A field study was conducted in an olive grove located in Northern Jordan during the growing season of 2015 to determine the population dynamics of this pest on two olive cultivars:Grossidi and Nebali Baladi,grown under rain-fed *** twigs were randomly sampled from the periphery of the canopy on each experimental tree at on month intervals throughout the entire *** sample was inspected thoroughly under a dissecting microscope in the laboratory for the presence of the immature and mature pit scale *** Grossidi olive cultivar was significantly more susceptible to *** than Nebali Baladi,as indicated by the development of higher populations throughout the *** was strong evidence that *** overwintered as *** generations were recorded per *** first generation occurred in February and March,followed by others from May to June and from August to November,*** first two generations were significantly larger than the third *** on these observations,the greatest abundance of crawlers occurs in mid-March and mid-May,and therefore,application of insecticides is recommended during this period of time,since crawlers lack a waxy covering on their body and thus facilitates the chemical penetration through the cuticle,making them more susceptible to ***,farmers are encouraged to cultivate the native Nebali Baladi cultivar rather than the more susceptible,introduced Grossidi cultivar in areas subject to heavy infestation.

关键词： Pollinia pollini Olea europaea olive cultivar population dynamic

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