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Nitric Oxide and Brassinosteroids Mediated Fungal Endophyte-Induced Volatile Oil Production Through Protein Phosphorylation Pathways in Atractylodes lancea Plantlets

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Journal of Integrative Plant Biology 2013年第11期55卷 1136-1146页

作者： Cheng-Gang Ren Chuan-Chao Dai Jiangsu Key Laboratory for Microbes and functional genomics Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources College of Life Sciences in Nanjing Normal University

Fungal endophytes have been isolated from almost every plant, infecting their hosts without causing visible disease symptoms, and yet have still proved to be involved in plant secondary metabolites accumulation. To decipher the possible physiological mechanisms of the endophytic fungus-host interaction, the role of protein phosphorylation and the relationship between endophytic fungus-induced kinase activity and nitric oxide （NO） and brassinolide （BL） in endophyte-enhanced volatile oil accumulation in Atractylodes lancea plantlets were investigated using pharmacological and biochemical approaches. Inoculation with the endophytic fungus Gilmaniella sp. ALl2 enhanced the activities of total protein phosphorylation, Ca2^-dependent protein kinase, and volatile oil accumulation in A. lancea plantlets. The upregulation of protein kinase activity could be blocked by the BL inhibitor brassinazole. Furthermore, pretreatments with the NO-specific scavenger cPTIO significantly reduced the increased activities of protein kinases in A. lancea plantlets inoculated with endophytic fungus. Pretreatments with different protein kinase inhibitors also reduced fungus-induced NO production and volatile oil accumulation, but had barely no effect on the BL level. These data suggest that protein phosphorylation is required for endophyte- induced volatile oil production in A. lancea plantlets, and that crosstalk between protein phosphorylation and the NO pathway may occur and act as a downstream signaling event of the BL pathway.

关键词： Atractylodes lancea endophytic fungus protein phosphorylation signal molecule volatile oi1.

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Origin and evolutionary analysis of the SARS-CoV-2 Omicron variant

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Journal of Biosafety and Biosecurity 2022年第1期4卷 33-37页

作者： Yamin Sun Wenchao Lin Wei Dong Jianguo Xu research Institute of Public Health Nankai UniversityTianjinPR China State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingPR China engineering and research center for microbial functional genomics and detection technology Ministry of EducationNankai University

The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has evolved rapidly into new variants throughout the *** Omicron variant has more than 50 mutations when compared with the original wild-type strain and has been identified globally in numerous *** this report,we analyzed the mutational profiles of several variants,including the per-site mutation rate,to determine evolutionary *** Omicron variant was found to have a unique mutation profile when compared with that of other SARS-CoV-2 variants,containing mutations that are rare in clinical ***,the presence of five mouse-adapted mutation sites suggests that Omicron may have evolved in a mouse *** in the Omicron receptor-binding domain(RBD)region,in particular,have potential implications for the ongoing pandemic.

关键词： SARS-CoV-2 Omicron variant Mouse-adapted mutation Reverse zoonosis

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A practical soil management to improve soil quality by applying mineral organic fertilizer

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Acta Geochimica 2017年第2期36卷 198-204页

作者： Leilei Xiao Qibiao Sun Huatao Yuan Bin Lian Key Laboratory of Coastal Biology and Utilization Yantai Institute of Coastal Zone ResearchChinese Academy of Sciences Jiangsu Key Laboratory for Microbes and functional genomics Jiangsu Engineering and Technology Research Center for MicrobiologyCollege of Life SciencesNanjing Normal University

Heavy use of chemical fertilizer causes increasing soil and environmental crisis, and the use of organic fertilizer increases obvious in recent years. In this study,mineral organic fertilizer(MOF) and compound fertilizer(CF) were applied in amaranth culture to explore the effects of these two kinds of fertilizers on soil quality and the potential function for CO_2 fixation. Some soil parameters were tested, e.g. p H value, organic carbon content, microbial biomass, urease activity, and available potassium content. In addition, some parameters of soil infiltration water were also determined, such as p H and HCO_3^- concentration. Experimental results showed that MOF improved soil quality and amaranth biomass and increased possible soil carbon *** the contrary, the utilization of CF worsened soil quality and made the soil acidize. These results suggested that MOF can partially replace CF to improve plant growth, soil quality and possible CO_2 sink.

关键词： Fertilizer management Mineral organicfertilizer Soil quality Natural way Carbon fixation

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Purification,characterization and synergism of a group of autolysis-related β-1,3-glucan hydrolases from the pilei of Coprinopsis cinerea fruiting bodies

Purification,characterization and synergism of a group of au...

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2015中国酶工程与糖生物工程学术研讨会

作者： Ya-jun Zhou Wen-ming Zhang Zhong-hua Liu Jun Wang Sheng Yuan Jiangsu Key Laboratory for Microbes and microbial functional genomics Jiangsu Engineering and Technology Research Center for Industrialization of Microbial ResourcesCollege of Life ScienceNanjing Normal University

＜正＞The genus Coprinopsis are characterized by rapid autolysis of the mature fruiting body（Muraguchi et al.2008;Kues 2000）.Prior to autolysis,the developing fruiting body undergoes remarkable morphogenesis:the stipe elongates and the pileus expands for efficient dispersal of *** of the mature pileus（Kues 2000）and stipe

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Massive‐scale genomic analysis reveals SARS‐CoV‐2 mutation characteristics and evolutionary trends

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mLife 2022年第3期1卷 311-322页

作者： Yamin Sun Min Wang Wenchao Lin Wei Dong Jianguo Xu research Institute of Public Health Nankai UniversityTianjinChina TEDA Institute of Biological Sciences and Biotechnology Nankai UniversityTianjinChina engineering and research center for microbial functional genomics and detection Ministry of EducationNankai UniversityTianjinChina State Key Laboratory for Infectious Disease Prevention and Control Chinese Center for Disease Control and PreventionNational Institute for Communicable Disease Control and PreventionBeijingChina research Units of Discovery of Unknown Bacteria and Function Chinese Academy of Medical SciencesBeijingChina

The severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2)pandemic resulted in significant societal ***,an in‐depth understanding of SARS‐CoV‐2 virus mutation and its evolution will help determine the direction of the COVID‐19 *** this study,we identified 296,728 de novo mutations in more than 2,800,000 high‐quality SARS‐CoV‐2 *** possible factors affecting the mutation frequency of SARS‐CoV‐2 in human hosts were analyzed,including zinc finger antiviral proteins,sequence context,amino acid change,and translation *** a result,we proposed that when adenine(A)and tyrosine(T)bases are in the context of AM(M stands for adenine or cytosine)or TA motif,A or T base has lower mutation ***,we hypothesized that translation efficiency can affect the mutation frequency of the third position of the codon by the selection,which explains why SARS‐CoV‐2 prefers AT3 codons *** addition,we found a host‐specific asymmetric dinucleotide mutation frequency in the SARS‐CoV‐2 genome,which provides a new basis for determining the origin of the SARS‐CoV‐***,we summarize all possible factors affecting mutation frequency and provide insights into the mutation characteristics and evolutionary trends of SARS‐CoV‐2.

关键词： de novo mutation evolutionary trends mutation characteristics mutation frequency SARS‐CoV‐2

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Characterization of Stipe Elongation of Mushroom Coprinus cinereus

Characterization of Stipe Elongation of Mushroom Coprinus ci...

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2014年第七届中国模式真菌研讨会

作者：章文明 Xiu-xiu Wu Ya-jun Zhou Zhong-hua Liu Weng Zhang Xin Niu Sheng Yuan Jiangsu Key Laboratory for Microbes and microbial functional genomics Jiangsu Engineering and Technology Research Center for Industrialization of Microbial ResourcesCollege of Life ScienceNanjing Normal University

In a previous experiment,we observed a acid-induced short-term wall extension in Flammulina velutipes apical stipes during a 15-rain period after been switched from a neutral to an acidic *** acid-induced stipe wall extension was eliminated by heating and reconstituted by a snail expansin-like protein,although we failed to isolate any endogenous expansin-like protein from *** because of its limited 1 mm fast elongation *** this study,we report that Coprinus cinereus stipes possess a 9 mm fast elongation apical region,which is suitable as a model material for wall extension *** elongating apical stipe shows two phases of

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Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification

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engineering 2022年第12期19卷 62-70页

作者： Yuting Shang Shuzhen Cai Qinghua Ye Qingping Wu Yanna Shao Xiaoying Qu Xinran Xiang Baoqing Zhou Yu Ding Moutong Chen Liang Xue Honghui Zhu Jumei Zhang Guangdong Provincial Key Laboratory of microbial Safety and Health&State Key Laboratory of Applied Microbiology Southern China&Institute of Microbiology Guangdong Academy of SciencesGuangzhou 510070China State Key Laboratory of Food Science and technology&National engineering research center for functional Foods&Synergetic Innovation center of Food Safety&Joint International research Laboratory on Food Safety School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food *** this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)*** dot nanobeads(QBs)served as fluorescence reporters,providing good detection *** customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product *** was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)*** linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test *** specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and *** detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis.

关键词： Salmonella Typhimurium Quantum dot nanobeads Lateral flow immunoassay strip Loop-mediated isothermal amplification Strand displacement probe

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Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

Laccase production by Phomopsis liquidambari B3 cultured wit...

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2014年第七届中国模式真菌研讨会

作者：周俊 Teng Yang Yan-Zhen Mei Li Kang Chuan-Chao Dai Jiangsu engineering and technology research center for Industrialization of microbial Resources Jiangsu Key Laboratory for Microbes and Functional GenomicsCollege of Life SciencesNanjing Normal University

Food waste is the most difficult waste to manage because the high treatment costs and the risk of environmental *** straw burning has become an increasingly discussed topic in recent years because the air pollution caused by ***,food waste and wheat straw contain high amounts of nutrient elements,efficient utilization of these waste can improve the environment by lessening airborne pollutant emission and the amount of waste that must be *** is well known that use of low cost and abundant waste materials in microbial fermentations can reduce product *** aimed to use these resources while improving laccase production by the endophytic fungus Phomopsis liquidambari *** cultured *** B3 in medium containing food waste and wheat straw as the main nitrogen and carbon sources,*** optimized the fermentation conditions by response surface methodology(RSM),using a Box-Behnken design for RSM I and a central composite design for RSM Ⅱ.Optimization resulted in an 11.07-fold(1.98-fold RSM I;5.59-fold RSM Ⅱ) increase in laccase yield compared with that before *** model was validated by mathematical evaluations and by comparisons between predicted and experimental *** optimized conditions,53.76%of lignin in wheat straw was *** optimizing fermentation conditions and using multiple bioresources,laccase production by this fungus was *** results provide the foundation for future research and for scaled-up laccase production.

关键词： Laccase Food waste Wheat straw Response surface methodology Phomopsis liquidambari B3

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Paralogous Ribosomal Protein L32-1 and L32-2 in Fission Yeast May Function Distinctively in Cellular Proliferation and Quiescence by Changing the Ratio of Rpl32 Paralogs

Paralogous Ribosomal Protein L32-1 and L32-2 in Fission Yeas...

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2014年第七届中国模式真菌研讨会

作者： Lei Sun Xiaowei Yang Feifei Chen Rongpeng Li Xuesong Li Zhenxing Liu Yuyu Gu Xiaoyan Gong 刘中华 Hua Wei Ying Huang Sheng Yuan Jiangsu Key Laboratory for Microbes and functional genomics Jiangsu Engineering and Technology Research Center for Industrialization of Microbial ResourcesCollege of Life ScienceNanjing Normal University

Fission yeast cells express Rp132-2 highly while Rp132-1 lowly in log phase;in contrast,expression of Rp132-1 raises and reaches a peak level while Rpi32-2 is downregulated to a low basic level when cells enter into stationary *** of Rp132-1 inhibits cell growth while overexpression of Rp132-2 does *** rpl32-2 impairs cell growth more severely than deleting rpl32-1 *** growth impaired by deleting either paralog can be rescued completely by reintroducing rpl32-2,but only partly by *** of Rp132-1inhibits cell division,yielding 4c DNA and multiple septa,while overexpressed Rp132-2 promotes

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Enhanced Nitrogen and Phosphorus Activation with an Optimized Bacterial Community by Endophytic Fungus Phomopsis liquidambari in Paddy Soil

Enhanced Nitrogen and Phosphorus Activation with an Optimize...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Mengjun Tang Qiang Zhu Fengmin Zhang Wei Zhang Jie Yuan Kai Sun Fangji Xu Chuanchao Dai Jiangsu Key Laboratory for Microbes and functional genomics Jiangsu Engineering and Technology Research Center for Industrialization of Microbial ResourcesCollege of Life SciencesNanjing Normal University

The endophytic fungus Phomopsis liquidambari play a key role in habitat adaptation of rice(Oryza sativa L.) with potential multiple ***,our previous published work on this subject remains ***,we performed a soil nutrient(nitrogen and phosphorus) transformation with related functional genes and elucidated how rhizosphere microbiota vary their response to *** interaction throughout the plant’s life cycle under field conditions by Illumina Miseq sequencing platforms in a nutrient-limited paddy *** results showed that *** symbiosis decreased the nitrogen and phosphorus loss by 531.24 mg and 558.12 mg per pot,***,we suggest that the application of *** altered the activation of soil nitrogen and phosphorus functional genes to accelerate nutrient turnover in the rice ***-throughput sequencing with cooccurrence network and species-related network analysis further revealed that *** colonization influenced the patterns of microbiota shift in the rhizosphere,especially during the heading *** led to an optimized microbial community through the promotion and inhibition of indigenous soil microbes with a higher level of available nutrient *** study strongly proposes *** symbiosis as a useful candidate for improving N and P acquisition and utilization.

关键词： rhizosphere microbiota whole developmental stages fungal endophyte network analysis rice soil nutrient

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