检索结果-南通市图书馆

Expression of phenylalanine ammonia lyase as an intracellularly free and extracellularly cell surface-immobilized enzyme on a gut microbe as a live biotherapeutic for phenylketonuria

引用

Science China(Life Sciences) 2023年第1期66卷 127-136页

作者： Yu Jiang Bingbing Sun Fenghui Qian Feng Dong Chongmao Xu Wuling Zhong Rui Huang Qiwei Zhai Yu Jiang Sheng Yang Shanghai Taoyusheng Biotechology Co. Ltd.Shanghai 201201China CAS Key Laboratory of synthetic Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Shanghai research and Development center of Industrial biotechnology Shanghai 201201China CAS Key Laboratory of Nutrition Metabolism and Food SafetyShanghai Institute of Nutrition and HealthChinese Academy of SciencesShanghai 200031China

Phenylketonuria(PKU),a disease resulting in the disability to degrade phenylalanine(Phe)is an inborn error with a 1 in 10,000 morbidity rate on average around the world which leads to *** an potential alternative to a protein-restricted diet,oral intake of engineered probiotics degrading Phe inside the body is a promising treatment,currently at clinical stage II(Isabella,et al.,2018).However,limited transmembrane transport of Phe is a bottleneck to further improvement of the probiotic’s ***,we achieved simultaneous degradation of Phe both intracellularly and extracellularly by expressing genes encoding the Phe-metabolizing enzyme phenylalanine ammonia lyase(PAL)as an intracellularly free and a cell surface-immobilized enzyme in Escherichia coli Nissle 1917(EcN)which overcomes the transportation *** metabolic engineering strategy was also combined with strengthening of Phe transportation,transportation of PAL-catalyzed trans-cinnamic acid and fixation of released *** of our final synthetic strain TYS8500 with PAL both displayed on the cell surface and expressed inside the cell to the Pah^(F263S)PKU mouse model reduced blood Phe concentration by 44.4%compared to the control Ec N,independent of dietary protein ***8500 shows great potential in future applications for PKU therapy.

关键词： phenylalanine ammonia lyase cell surface display phenylketonuria TYS8500 oral administration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ordered mesoporous carbon as an efficient heterogeneous catalyst to activate peroxydisulfate for degradation of sulfadiazine

引用

Chinese Chemical Letters 2022年第5期33卷 2747-2752页

作者： Zhi-Ling Li Di Cao Hao Cheng Fan Chen Jun Nan Bin Liang Kai Sun Cong Huang Ai-Jie Wang State Key Laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHarbin 150090China School of Civil&Environmental engineering Harbin Institute of Technology(Shenzhen)Shenzhen 518055China School of Ecology and Environment Northwestern Polytechnical UniversityXi’an 710129China Key Lab of Structures Dynamic Behavior and Control of China ministry of education School of Civil EngineeringHarbin Institute of TechnologyHarbin 150090China National Technology Innovation center of synthetic Biology Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China

Catalytic potential of carbon nanomaterials in peroxydisulfate(PDS)advanced oxidation systems for degradation of antibiotics remains poorly *** study revealed ordered mesoporous carbon(type CMK)acted as a superior catalyst for heterogeneous degradation of sulfadiazine(SDZ)in PDS sys-tem,with a first-order reaction kinetic constant(k)and total organic carbon(TOC)mineralization efficiency of 0.06 min^(–1) and 59.67%±3.4%within 60min,*** catalyzed PDS system exhibited high degradation efficiencies of five other sulfonamides and three other types of antibiotics,verifying the broad-degradation capacity of *** neutral pH conditions,the optimal catalytic parameters were an initial SDZ concentration of 44.0mg/L,CMK dosage of 0.07g/L,and PDS dosage of 5.44mmol/L,respectively.X-ray photoelectron spectroscopy and Raman spectrum analysis confirmed that the defect structure at edge of CMK and oxygen-containing functional groups on surface of CMK were major active sites,contributing to the high catalytic *** radical quenching analysis revealed that both SO_(4)•−and•OH were generated and participated in catalytic *** addition,direct electron transfer by CMK to activate PDS also occurred,further promoting catalytic *** of SDZ molecule was optimized using density functional theory,and the possible reaction sites in SDZ molecule were calculated using Fukui *** ultra-high-performance liquid chromatography(UPLC)–mass spectrometry(MS)/MS analysis,three potential degradation pathways were proposed,including the direct removal of SO_(2)molecules,the 14S-17N fracture,and the 19C-20N and 19C-27N cleavage of the SDZ *** study demonstrated that ordered mesoporous carbon could work as a feasible catalytic material for PDS advanced oxidation during removal of antibiotics from wastewater.

关键词： Ordered mesoporous carbon CMK Sulfadiazine Peroxydisulfate Degradation pathway Density functional theory Physicochemical properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Highly Efficient Heavy-Metal-Ion Removal from Shellfish Processing Liquid with Low Protein and Polysaccharide Loss by Hybrid Mesoporous Silica Diol-APDC-SBA15

引用

Journal of Ocean University of China 2023年第1期22卷 221-228页

作者： QI Yanxia SONG Yang LIU Chang QI Shizhe WANG Haibo CAO Jijuan ZHAO Qiancheng College of food Science and engineering Dalian Ocean UniversityDalian 116023China Liaoning Provincial Aquatic Products Analyzing Testing and Processing Technology Scientific Service CenterDalian 116023China Key Laboratory of biotechnology and Bioresources Utilization of ministry of education Dalian Minzu UniversityDalian 116600China Collaborative Innovation center of Seafood Deep Processing Dalian Polytechnic UniversityDalian 116034China Dalian Key Laboratory of Marine Bioactive Substances Development and High Value Utilization Dalian 116034China

Heavy metal ions in shellfish products are harmful to human health,and their removal with low nutrient loss remains ***,a new type of mesoporous silica(SBA15),modified internally with ammonium pyrrolidine dithiocarbamate(APDC)and externally with alkyl-diol groups,which was named as Diol-APDC-SBA15,was successfully developed and characterized by powder X-ray diffraction patterns,nitrogen adsorption,and Fourier transform infrared *** solutions with lead,chromium,cadmium,and copper were used to investigate the adsorption capacity of ***-APDC-SBA15 was adopted to remove heavy metals from cooking liquids of clams(Ruditapes philippinarum),hydrolysate liquids of oysters(Ostrea gigas Thunberg),and polysaccharide solution from the cooking liquid of *** efficiencies of removing heavy metal ions and the loss rates of proteins and polysaccharides were *** results showed that the adsorption capacities of Diol-APDCSBA15 for Pb,Cr,Cd,and Cu in standard heavy-metal solutions were 161.4,166.1,29.6,and 60.2mgg^(−1),*** removal efficiency of Diol-APDC-SBA15 for Pb in the three shellfish processing liquids ranged from 60.5%to 99.6%.The Cr removal efficiency was above 99.9%in the oyster hydrolysate ***,the percentages of polysaccharide loss were 5.5%and 3.7%in the cooking liquid of clam and polysaccharide solution,respectively,and the protein loss was 1.2%in the oyster hydrolysate ***,the Diol-APDC-SBA15 material exhibits a great potential application in the removal of heavy metals from shellfish processing liquids with low losses of proteins and polysaccharides.

关键词： heavy-metal removal modified mesoporous silica shellfish processing liquid protein polysaccharide

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhanced production of L-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans

引用

synthetic and Systems biotechnology 2022年第2期7卷 730-737页

作者： Li Liu Yue Chen Shiqin Yu Jian Chen Jingwen Zhou National engineering Laboratory for Cereal Fermentation Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China School of biotechnology and Key Laboratory of Industrial biotechnology Ministry of EducationJiangnan University1800 Lihu RoadWuxiJiangsu214122China Science center for Future foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Provisional research center for Bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China

L-Sorbose is an essential intermediate for the industrial production of vitamin C(L-ascorbic acid).However,the formation of fructose and some unknown by-products significantly reduces the conversion ratio of D-sorbitol to *** study aimed to identify the key D-sorbitol dehydrogenases in Gluconobacter oxydans WSH-003 by gene ***,a total of 38 dehydrogenases were knocked out in *** WSH-003,and 23 dehydrogenase-deficient strains could increase L-sorbose ***-30,wherein a pyrroloquinoline quinone-dependent glucose dehydrogenase was deleted,showed a significant reduction of a by-product with the extension of fermentation *** addition,the highest conversion ratio of 99.60%was achieved in *** MD-16,in which 16 different types of dehydrogenases were inactivated ***,the gene vhb encoding hemoglobin was introduced into the *** titer of L-sorbose was 298.61 g/L in a 5-L *** results showed that the systematic engineering of dehydrogenase could significantly enhance the production of L-sorbose.

关键词： D-sorbitol dehydrogenase Gluconobacter oxydans L-ascorbic acid Metabolic engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Elimination of ethyl carbamate in fermented foods

引用

food Bioscience 2022年第3期47卷 121-131页

作者： Yu Chen Weizhu Zeng Fang Fang Shiqin Yu Jingwen Zhou National engineering Laboratory for Cereal Fermentation Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Key Laboratory of Industrial biotechnology Ministry of Education and School of BiotechnologyJiangnan University1800 Lihu RoadWuxiJiangsu214122China Science center for Future foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Provisional research center for Bioactive Product Processing Technology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China

Ethyl carbamate (EC) is a common harmful by-product in fermented foods and can be derived from raw materials or formed during the fermentation or even in the *** content can be affected by temperature,microorganisms,technological processes,and other *** this review,we summarized the technologies and their applications for EC elimination in fermented foods in the light of upstream processes,such as raw material pretreatment,lower temperature,breeding microorganisms,and exogenous substances,to the downstream enzymatic method and physical *** review covers different strategies to eliminate EC,such as strain breeding,genetic engineering,enzyme,and physicochemical *** comparing these strategies,practical strategies for the efficient elimination of EC in different fermented foods on an industrial scale have been discussed and proposed in consideration of flavor,food safety and feasibility of an industrial application.

关键词： Fermented foods Alcoholic beverages Ethyl carbamate Elimination strategies Nitrogen metabolism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Integrated microalgae culture with food processing waste for wastewater remediation and enhanced biomass productivity

引用

Chinese Chemical Letters 2023年第2期34卷 636-643页

作者： Angela Paul Peter Kit Wayne Chew Apurav Krishna Koyande Heli Siti Halimatul Munawaroh Amit Bhatnagar Yang Tao Chihe Sun Fubao Sun Zengling Ma Pau Loke Show Department of Chemical and Environmental engineering Faculty of Science and EngineeringUniversity of Nottingham MalaysiaJalan Broga43500 SemenyihSelangor Darul EhsanMalaysia Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection Wenzhou UniversityWenzhou 325035China Study Program of Chemistry Department of Chemistry EducationFaculty of Mathematics and Science EducationUniversitas Pendidikan IndonesiaBandung 40154Indonesia Department of Separation Science LUT School of Engineering ScienceLUT UniversitySammonkatu 12FI 50130MikkeliFinland College of food Science and Technology Nanjing Agricultural UniversityNanjing 210095China Key Laboratory of Industrial biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China Department of Sustainable engineering Saveetha School of EngineeringSIMATSChennai 602105India

Waste generation from food manufacturing facilities poses a serious hazard like environmental degradation, water pollution, and land pollution due to its high nutrient composition. Specifically, solid waste(powder) disposal requires additional energy sources in terms of scientific treatment, structured collection, and disposal packaging according to the safety regulation. Thus, this research discusses the viewpoint of integrating food processing waste as an organic carbon source with BG-11 medium for Chlorella vulgaris(FSP-E) growth. The food processing waste powders investigated in this study were obtained from milk, and biscuit manufacturing facilities. The culture medium was modified by combining both BG-11 and food processing waste powders to identify the optimal algal growth and biochemical *** to the microalgae grown in BG-11 alone(IBG), the combination of biscuit waste and IBG produced higher biomass concentration(44%), with increased lipid(11%), protein(20%), and carbohydrate(57%) contents. Chlorella vulgaris was able to uptake nutrients from the culture medium with combination of food processing waste and IBG thus enhancing its growth. The results obtained also indicate that an integrated culture system using food processing waste and synthetic sources can generate energy out of waste by improving the bio-composition of the microalgae biomass.

关键词： Medium contamination food processing Waste powder Organic medium Waste remediation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Advances in metabolic engineering of yeasts for the production of fatty acid-derived hydrocarbon fuels

引用

Green Chemical engineering 2022年第4期3卷 289-303页

作者： Ran Lu Tian-Qiong Shi Lu Lin Rodrigo Ledesma-Amaro Xiao-Jun Ji He Huang State Key Laboratory of Materials-Oriented Chemical engineering College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing211816China School of food Science and Pharmaceutical engineering Nanjing Normal UniversityNanjing210046China Department of Bioengineering and Imperial College Centre for synthetic Biology Imperial College LondonLondonSW72AZUnited Kingdom

The reliance of the transport sector on fossil fuels has led to increasing concerns related to sustainability and environmental *** petroleum shortages and global climate change caused by greenhouse gas emissions have given an impetus to the development of renewable and low-carbon energy *** acidderived hydrocarbons are an attractive drop-in biofuel to substitute fossil fuels consumed by the transport sector,especially heavy *** to traditional biofuels such as ethanol and biodiesel,fatty acidderived hydrocarbons have higher energy density and better compatibility with existing storage and transport *** a common microbial platform for biofuel production,yeasts have been engineered to produce fatty acid-derived ***,we attempted to comprehensively review the latest advances in metabolic engineering strategies for the production of fatty acid-derived hydrocarbons in the yeasts Saccharomyces cerevisiae and Yarrowia *** heterologous hydrocarbon synthesis pathways and combinational metabolic engineering strategies have been summarized,followed by a discussion of future research directions for the development of yeasts as industrial hydrocarbon producers.

关键词： Biofuel Metabolic engineering Fatty acid Hydrocarbon Yeast

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

research progress on classification, sources and functions of dietary polyphenols for prevention and treatment of chronic diseases

引用

未来食品学报(英文) 2023年第4期3卷 289-305页

作者： Wei Li Haihong Chen Bing Xu Yi Wang Canyang Zhang Yong Cao Xinhui Xing Institute of Biopharmaceutical and Health engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China b Institute of Biomedical Health Technology and engineering Shenzhen Bay LaboratoryShenzhen 440300China Key Laboratory for Industrial Biocatalysis Ministry of EducationInstitute of Biochemical EngineeringDepartment of Chemical EngineeringBeijing 100084China College of food Science South China Agricultural UniversityGuangzhou 510642China center for synthetic and Systems Biology Tsinghua UniversityBeijing 100084China

Dietary polyphenols are the most abundant antioxidants in daily diet and widely distributed in plants and plant-based foods,including vegetables,fruits,cereals,nuts,and beverages(e.g.,tea,coffee,red wine).Over the past decades,there has been rising scientific interest in their diverse benefits to human health,since dietary polyphenols display various bioactivities,such as antioxidant activity,anti-inflammatory activity,anticancer,anti-microbial activity,neuro-protective activity,among *** studies have demonstrated that diet with polyphenols is strongly related to decrease the risk of chronic *** this review,firstly,we systematically summarize classification and sources of dietary polyphenols,and focus on the current evidences defining their function and mechanism on improving chronic diseases,including diabetes,obesity,hypertension,***,future directions of dietary polyphenols are ***,despite the physiological effects of dietary polyphenols have been widely convinced in vitro and in vivo,human trials show inconsistent evidence with their beneficial effects in chronic diseases administration,so as to their unclear molecular *** clinical studies are needed to provide clear evidence on dietary polyphenols against chronic *** general,this paper provides comprehensive review of the sources and compositions of dietary polyphenols from plant materials,and presents an overview of the evidence on dietary polyphenols against chronic diseases,which may offer some constructive inspirations for further research on dietary polyphenols and industrial application in the food and medicine industry.

关键词： Dietary polyphenols Source Chronic diseases Diabetes Obesity Hypertension Hyperuricemia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gut lumen-targeted oral delivery system for bioactive agents to regulate gut microbiome

引用

Journal of Future foods 2022年第4期2卷 307-325页

作者： Junyu Liu Xiaobin Li Xiaopeng Zhang Runming Wang Sanyang Han Peiwu Qin Xin-Hui Xing Canyang Zhang Institute of Biopharmaceutical and Health engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biochemical engineering Department of Chemical EngineeringKey Laboratory for Industrial BiocatalysisMinistry of EducationCenter for Synthetic and Systems BiologyTsinghua UniversityBeijing 100084China Institute of Biomedical Health Technology and engineering Shenzhen Bay LaboratoryShenzhen 518055China

Gut microbiome(GM)is closely related to the overall health status for the human *** dysfunction of microbiome can lead to many diseases such as inflammation,cancer and neurodegenerative ***,it's of great importance to develop effective strategy to regulate *** lumen-targeted oral delivery system(GLT-ODS)has been extensively investigated and widely used in food science and engineering in these years,due to the targeted delivery property,controlled release profile,high biocompatibility and enhanced bioavailability of ***,we comprehensively summarized the current advances in GLT-ODS for bioactive ***,we systematically summarize the GLT-ODS for pre-agents(prebiotics and probiotics)and anti-agents(antibiotics and bacteriophages)according to the type of *** in-depth discussion of representative researches,we refined the limitations of existing research in carrier material and target selection,and drew a blueprint for the future technological research and *** believe that GLT-ODS will become a safe,efficient,simple and precise GM management strategy for improvement of health and shine in the development of precision food and pharmaceutical engineering in future.

关键词： Gut lumen-targeted Oral delivery systems Bioactive agents Gut microenvironment food engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recent progress of sugar amino acids: synthetic strategies and applications as glycomimetics and peptidomimetics

引用

Chinese Chemical Letters 2015年第8期26卷 922-930页

作者： Guang-Zong Tian Xiao-Li Wang Jing Hu Xue-Bin Wang Xiao-Qiang Guo Jian Yin Key Laboratory of Carbohydrate Chemistry and biotechnology ministry of education School of Biotechnology Jiangnan University Synergetic Innovation center of food Safety and Nutrition Jiangnan University Wuxi Medical School Jiangnan University

In order to meet the increasing demands for the development of large varieties of new molecules for discovering new drugs and materials, organic chemists are developing many novel multifunctional building blocks, which are assembled rationally to create ‘nature-like＇ and yet unnatural organic molecules with well-defined structures and useful properties. Sugar amino acids（SAAs）, the carbohydrate derivatives bearing both amino and carboxylic acid functional groups, are important ones of these multifunctional building blocks, which can be used to create novel materials with potential applications as glycomimetics and peptidomimetics. This review will focus on recent synthetic strategies of SAAs and their applications in creating large number of structurally diverse glycomimetics and peptidomimetics.

关键词： structurally carboxylic oligomer blocks assembled Fmoc amide carbohydrate diverse protected

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：