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Journal of Pharmaceutical Analysis 2022年第1期12卷 113-121页

作者： Juan Tan Shiyue Wu Qingqing Cai Yi Wang Pu Zhang engineering research center for biotechnology of active substances Ministry of EducationChongqing Key Laboratory of Green Synthesis and ApplicationsCollege of ChemistryChongqing Normal UniversityChongqing401331China Chongqing research center for Pharmaceutical engineering College of PharmacyChongqing Medical UniversityChongqing400016China

Regulating the catalytic activity of nanozymes is significant for their applications in various ***,we demonstrate a new strategy to achieve reversible regulation of the nanozyme’s activity for sensing *** strategy involves the use of zero-dimensional MoS;quantum dots(MQDs)as the building blocks of nanozymes which display very weak peroxidase(POD)-like ***,such POD-like activity of the MQDs largely enhances in the presence of Fe;while diminishes with the addition of captopril *** investigations identify the mechanism of Fe;-mediated aggregation-induced enhancement of the POD-like activity and the inhibitory effect of captopril on the enhancement,which is highly dependent on their *** on this finding,a colorimetric method for the detection of captopril is *** sensing approach exhibits the merits of simplicity,rapidness,reliability,and low cost,which has been successfully applied in quality control of captopril in pharmaceutical ***,the present sensing platform allows smartphone read-out,which has promising applications in point-of-care testing devices for clinical diagnosis and drug analysis.

关键词： Nanozyme Aggregation Peroxidase Captopril Colorimetric detection Smartphone

A Robust N-Terminal Pro-Brain Natriuretic Peptide Assay for Clinical Diagnosis of Heart Failure in Elderly Patients

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Journal of Analysis and Testing 2024年第1期8卷 74-82页

作者： Xue Gong Jiangling Wu Jiajia Zhang Zhongwei Jiang Yi Wang Pu Zhang engineering research center for biotechnology of active substances(Ministry of Education) Chongqing Key Laboratory of Green Synthesis and ApplicationsCollege of ChemistryChongqing Normal UniversityChongqing 401331China Chongqing research center for Pharmaceutical engineering College of PharmacyChongqing Medical UniversityChongqing 400016China Department of Clinical Laboratory University Town Hospital of Chongqing Medical UniversityChongqing 401331China

Heart failure(HF)is a clinical hallmark of cardiovascular syndrome leading to substantial morbidity,repeated hospitalization,and enormous healthcare *** elderly populations continue to increase,biomarker-guided diagnosis and treatment for age-related cardiac decline have become *** evidence demonstrates that N-terminal proBNP(NT-proBNP)can provide a unique window into the diagnosis and risk stratification with ***,a simple yet robust aptasensor is developed for on-site recognizing the NT-proBNP by its targeting aptamer,thus achieving the accurate diagnosis of *** aptasensing system is prepared by absorbing the fluorophore-labeled aptamer strand onto the graphene oxide(GO),leading to efficient quenching without possible off-site signal *** aptamer strand can specifically identify target NT-proBNP molecules via a versatile conformational transformation,resulting in the desorption of the aptamer-NTproBNP complexes from GO and re-generation fluorescence signal,thus allowing sensitive detection of NT-proBNP in 37 clinical blood *** advantage of the high specificity of aptamer-guaranteed recognitions of NT-proBNP,this aptasensor system readily achieves better diagnostic performance for HF than commercially adopted chemiluminescence immunoassay(Siemens,CLIA)in hospitals in terms of accuracy(89.2%vs 83.8%),specificity(89.5%vs 84.2%),and positive predictive value(88.9%vs 83.3%).This work provides a stable option for the diagnosis and treatment of elderly HF-related diseases inclinics.

关键词： NT-proBNP Graphene oxide Aptamer Heart failure Clinical diagnosis

Enhanced catalytic oxidation of dichloromethane by a surfactant-modified CeO_(2)@TiO_(2) core-shell nanostructured catalyst

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Journal of Rare Earths 2023年第7期41卷 1031-1041,I0003页

作者： Mengjie Fan Qingping Zou Jining Liu Yingwen Chen Jianliang Zhu Shubao Shen research and Development center for Watershed Environmental Eco-engineering Beijing Normal UniversityZhuhai 519087China College of biotechnology and Pharmaceutical engineering Nanjing Tech UniversityNanjing 210009China

Surfactant-modified CeO_(2)@TiO_(2) core-shell nanostructure catalysts were prepared by coprecipitation with the addition of sodium dodecyl sulfonate(SDS),and their catalytic oxidation of dichloromethane(DCM) was studied.A 90% DCM conversion efficiency is obtained at 300℃ with the CeO_(2)@TiO_(2)SDS catalyst,and its catalytic stability in the 55 h test period is better than that of Ce/TiO_(2) and CeO_(2)@TiO_(2).Based on the characterization of CeO_(2)@TiO_(2)SDS,the dispersion of active components is promoted due to the inhibition of crystal growth with the introduction of *** improvement of surface acidity and redox capacity is beneficial to the enhancement of catalytic *** higher adsorbed oxygen content on the surface of the CeO_(2)@TiO_(2)SDS catalyst is responsible for the better catalytic ***,a novel method was developed to design catalytic oxidation catalysts for the treatment of chlorinated volatile organic compounds in future applications.

关键词： Surfactant Oxidation Cerium titanium system Core-shell structure catalyst Dichloromethane Rare earths

Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2012年第4期13卷 267-273页

作者： Jun-liang WANG Qing XIA An-ping ZHANG Xiao-yan HU Chun-mian LIN International Joint research center for Persistent Toxic substances College of Biological and Environmental EngineeringZhejiang University of TechnologyHangzhou 310014China Zhejiang Province Environmental Radiation Monitoring center Hangzhou 310012China

The widespread use of organophosphorus pesticides(OPs) poses a great threat to human health and has made the detection of OP residues in food an important task,especially in view of the fact that easy and rapid detection methods are *** OPs have inhibitory effects on the activity of α-naphthyl acetate esterase(ANAE) in plants,in this work we evaluated the possibility of detecting OPs in vegetables with ANAE extracted from commercial *** limits of detection(LODs) obtained for methamidophos,dichlorvos,phoxim,dimethoate,and malathion in lettuce samples with crude ANAE were 0.17,0.11,0.11,0.96,and 1.70 mg/kg,*** on the maximum residue limits(MRLs) for OPs in food stipulated by Chinese laws which are 0.05,0.20,0.05,1.00,and 8.00 mg/kg for methamidophos,dichlorvos,phoxim,dimethoate,and malathion,respectively,the esterase inhibition method with crude ANAE had sufficient sensitivity to detect the residues of dichlorvos,dimethoate,and malathion in lettuce,but it could not be used to guarantee the safety of the same samples if methamidophos or phoxim residue was *** sensitivity of the method was improved by the use of esterase purified by ammonium sulfate *** LODs obtained for methamidophos and phoxim with purified esterase were lower than the MRLs for these OPs in *** is a very promising method for the detection of OP residues in vegetables using crude or purified esterase because of its cheapness,sensitivity,and convenience.

关键词： Organophosphorus pesticides (OPs) α-Naphthyl acetate esterase (ANAE) Limit of detection (LOD) Enzvme inhibition Maximum residue levels (MRLs)

Gas stunning with CO_2 affected meat color,lipid peroxidation, oxidative stress, and gene expression of mitogen-activated protein kinases, glutathione S-transferases,and Cu/Zn-superoxide dismutase in the skeletal muscles of broilers

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Journal of Animal Science and biotechnology 2018年第3期9卷 738-749页

作者： Lei Xu Haijun Zhang Hongyuan Yue Shugeng Wu Haiming Yang Zhiyue Wang Guanghai Qi Key Laboratory of Feed biotechnology of Ministry of Agriculture & National Engineering Research Center of Biological Feed Feed Research Institute Chinese Academy of Agricultural Sciences College of Animal Science and Technology Yangzhou University

Background: Meat color and lipid peroxidation are important traits related to meat quality. CO_2 concentration is a critical factor that can affect meat quality in the commercial use of gas stunning(GS). However, the effect and mechanism of CO_2 stunning on meat color and lipid peroxidation during long-term storage remain poorly studied. We aimed to study the effects of GS methods, especial y CO_2 concentration, on meat color and meat lipid peroxidation in broilers during long-term storage at 4 °C and to explore the potential mechanism of meat color change via lipid peroxidation and the inner lipid peroxide scavenging ***: Eighteen broilers were sacrificed after exposure to one of the following gas mixtures for 90 s: 40% CO_2+21% O_2+ 39% N2(G40%), 79% CO_2+ 21% O_2(G79%), or no stunning(0% CO_2, control). Meat color, serum variables,enzyme activities, and the gene expression of mitogen-activated protein kinase(MAPK), nuclear factor-erythroid2-related factor 2(Nrf2), glutathione S-transferase(GST) and superoxide dismutase(SOD) were determined.(Continued on next page)Results: The concentrations of serum triiodothyronine(T3, P = 0.03) and the ratio of serum free triiodothyronine/free thyroxine(FT3/FT4, P active substances(TBARS)3 d(P < 0.01) and TBARS6 d(P = 0.01) in breast meat and the TBARS3 din thigh meat(P < 0.01) were increased in the40% CO_2 group compared with the control group. Serum T3 was negatively correlated with TBARS6 dboth in the breast and thigh meat(r =-0.63, P < 0.01 and r =-0.47, P = 0.05 respectively). T3/T4 was negatively correlated with TBARS6 din the breast meat and in the thigh meat(r =-0.57, P = 0.01; and r =-0.53, P = 0.03 respectively). Compared with the control group, Lightness(L*)1 d(P = 0.03) and L*9 d(P < 0.01) were increased, whereas total chromatic abe

关键词： Antioxidant enzyme Broiler Controlled atmosphere stunning Gas stunning Lipid peroxidation MAPK/Nrf2/ARE Meat color Oxidative stress

Genomic analysis of Asian honeybee populations in China reveals evolutionary relationships and adaptation to abiotic stress

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Genomic analysis of Asian honeybee populations in China reve...

engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae

2021年中国（广西梧州）蜂业博览会暨全国蜂产品市场信息交流会

作者： Peng Shi Jun Zhou Huali Song Yujuan Wu Lan Lan Xiangyou Tang Zhengang Ma Charles R.Vossbrinck Bettina Vossbrinck Zeyang Zhou Jinshan Xu College of Life Sciences Chongqing Normal University engineering research center of biotechnology for active substances Ministry of Education State Key Laboratory of Silkworm Genome Biology Southwest University

The geographic and biological diversity of China has resulted in the differential adaptation of the eastern honeybee,Apis cerana,to these varied *** were collected from 14 locations in *** genomes were sequenced,and nucleotide polymorphisms were identified at more than 9 million *** STRUCTURE and principal component analysis placed the bees into seven *** analysis groups the honeybees into many of the same clusters with high bootstrap values(91%-100%).Populations from Tibet and South Yunnan are sister taxa and together represent the earliest diverging lineage included in this *** propose that the evolutionary origin of *** in China was in the southern region of Yunnan Province and expanded from there into the southeastern regions and into the north-eastern mountain *** Cold-Temperate West Sichuan Plateau and Tropical Diannan populations were compared to identify genes under adaptive selection in these two *** enrichment analysis showing genes under selection,including the Hippo signaling pathway,GABAergic pathway,and trehalose-phosphate synthase,indicates that most genes under selection pressure are involved in the process of signal transduction and energy ***-PCR analysis reveals that one gene under selection,the AcVIAAT gene,involved in the GABAergic pathway,is responding to cold temperature *** homologous recombination,we show that the AcVIAAT gene is able to replace the CNAG1904 gene in the fungus Cryptococcus neoformans and that it makes the fungus less sensitive to conditions of oxidative stress and variations in *** results contribute to our understanding of the evolutionary origin of *** in China and the molecular basis of environmental adaptation.

关键词： adaptation Apis cerana evolution population signal induction stress

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Synthetic and Systems biotechnology 2022年第4期7卷 1056-1063页

作者： Qian Zhang Ning Li Yunbin Lyv Shiqin Yu Jingwen Zhou Science center for Future Foods Jiangnan University1800 Lihu RoadWuxiJiangsu214122China School of biotechnology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China engineering research center of Ministry of Education on Food Synthetic biotechnology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China Jiangsu Province engineering research center of Food Synthetic biotechnology Jiangnan University1800 Lihu RoadWuxiJiangsu214122China

As a potential substitute for fatty acids,common low-cost oils could be used to produce acetyl-CoA derivatives,which meet the needs of low-cost industrial ***,oils are hydrophobic macromolecules and cannot be directly transported into *** this study,caveolin was expressed in Saccharomyces cerevisiae to absorb exogenous *** expression of caveolin fused with green fluorescent protein showed that caveolin mediated the formation of microvesicles in *** and the addition of 5,6-carboxyfluorescein showed that caveolae had the ability to transport exogenous substances into *** intracellular and extracellular triacylglycerol levels were then detected after the addition of soybean oil pre-stained with Nile Red,which proved that caveolae had the ability to absorb the exogenous ***,caveolin for oils absorption and lipase from Bacillus pumilus for oil hydrolysis were co-expressed in the naringenin-producing Saccharomyces cerevisiae strain,resulting in naringenin production increasing from 222 mg/g DCW(dry cell weight)(231 mg/L)to 269 mg/g DCW(241 mg/L).These results suggested that the caveolin-mediated transporter independent oil transport system would provide a promising strategy for the transport of hydrophobic substrates.

关键词： Oil Caveolin-1 β-Oxidation (2S)-Naringenin Saccharomyces cerevisiae

Immune modulatory function of abundant immune-related microRNAs in microvesicles from bovine colostrum

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Protein & Cell 2013年第3期4卷 197-210页

作者： Qi Sun Xi Chen Jianxiong Yu Ke Zen Chen-Yu Zhang Liang Li Jiangsu engineering research center for microRNA Biology and biotechnology State Key Laboratory of Pharmaceutical BiotechnologyNanjing University School of Life SciencesNanjing 210093China Department of Virology University of California School of Public HealthBerkeleyCA 94720USA

Colostrum provides essential nutrients and immunologically active factors that are beneficial to *** previous work demonstrated that milk contains large amounts of miRNA that is largely stored in milk-derived microvesicles(MVs).In the present study,we found that the MVs from colostrum contain signifi cantly higher levels of several immune-related *** hypothesized that the colostrum MVs may transfer the immune-related miR-NAs into cells,which contribute to its immune modulatory *** isolated colostrum MVs by ultracentrifugation and demonstrated several immune modulation features associated with *** also provide evidence that the physical structure of milk-derived MVs is essential for transfer miRNAs and following immune modulation ***,we found that colostrum powder-derived MVs also contains higher levels of immune-related miRNAs that display similar immune modulation *** together,these results show that MV-containing immunerelated miRNAs may be a novel mechanism by which co-lostrum modulates body immune response.

关键词： colostrum miRNAs microvesicles immune modulation

Hepatic metabolomics combined with network pharmacology to reveal the correlation between the anti-depression effect and nourishing blood effect of Angelicae Sinensis Radix

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Chinese Journal of Natural Medicines 2023年第3期21卷 197-213页

作者： GONG Wenxia XU Shaohua SONG Yapeng ZHOU Yuzhi QIN Xuemei Modern research center for Traditional Chinese Medicine Shanxi UniversityTaiyuan 030006China Key Laboratory of Chemical Biology and Molecular engineering of Ministry of Education Taiyuan 030006China Key Laboratory of Effective substances research and Utilization in TCM of Shanxi Province Taiyuan 030006China

Angelicae Sinensis Radix(AS)is reproted to exert anti-depression effect(ADE)and nourishing blood effect(NBE)in a rat model of *** correlation between the two therapeutic effects and its underlying mechanisms deserves further *** current study is designed to explore the underlying mechanisms of correlation between the ADE and NBE of AS based on hepatic metabonomics,network pharmacology and molecular *** to metabolomics analysis,30 metabolites involved in 11 metabolic pathways were identified as the potential metabolites for ***,principal component analysis and correlation analysis showed that glutathione,sphinganine,and ornithine were related to pharmacodynamics indicators including behavioral indicators and hematological indicators,indicating that metabolic pathways such as sphingolipid metabolism were involved in the ADE and NBE of ***,a target-pathway network of depression and blood deficiency syndrome was constructed by network pharmacology analysis,where a total of 107 pathways were ***,37 active components obtained from Ultra Performance Liquid Chromatography-Triple-Time of Flight Mass Spectrometer(UPLC-Triple-TOF/MS)in AS extract that passed the filtering criteria were used for network pharmacology,where 46 targets were associated with the ADE and NBE of *** enrichment analysis further indicated the involvement of sphingolipid metabolism in the ADE and NBE of *** docking analysis indciated that E-ligustilide in AS extract exhibited strong binding activity with target proteins(PIK3CA and PIK3CD)in sphingolipid *** analysis by Western blot verified that AS regulated the expression of PIK3CA and PIK3CD on sphingolipid *** results demonstrated that sphingolipid metabolic pathway was the core mechanism of the correlation between the ADE and NBE of AS.

关键词： Angelicae Sinensis Radix Blood deficiency syndrome Depression Metabonomics Network pharmacology Sphingolipid metabolism

Characterization of aroma profiles and aroma-active compounds in high-salt and low-salt shrimp paste by molecular sensory science

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Food Bioscience 2022年第1期45卷 174-184页

作者： Jing Yu Kuan Lu Jiwei Zi Xihong Yang Wancui Xie College of Marine Science and Biological engineering Qingdao University of Science and TechnologyQingdaoShandong266042PR China Qingdao engineering research center of Marine Bioactive substances and Precision Nutrition QingdaoShandong266042PR China Shandong Provincial Key Laboratory of Biochemical engineering Shandong266042PR China Qingdao Keda Future biotechnology Co. Ltd.QingdaoShandong266042PR China

Flavor characteristics of raw shrimp,high-salt and low-salt shrimp paste were evaluated and compared by sensory analysis,headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS)and solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS).In addition,aroma profiles and aroma-active compounds in these three samples were analyzed by solvent-assisted flavor evaporation-gas chromatography-olfactometry-mass spectrometry(SAFE-GC-O-MS).Results of sensory evaluation revealed that these three samples showed significant differences in sensitivity to the W2W,W2S,W1S and W1W sensors of the electronic nose(pactive compounds were detected by ***-salt shrimp was shown to contain more acids,aldehydes and heterocyclic compounds,which resembled cheese,roasted and meaty *** contrast,low-salt shrimp paste contained more esters,which was similar to the umami aroma profile of raw ***,pyrazines were only produced in fermented shrimp paste samples,suggesting that they were produced during the fermentation process and they may contribute unique roasted and meaty aromas,which were important for the overall aroma characteristics of shrimp paste.

关键词： shrimp Paste Salt content Molecular sensory science Aroma-active compounds