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Journal of Animal Science and Biotechnology 2020年第2期11卷 327-347页

作者： Emilio Mármol-Sánchez Yuliaxis Ramayo-Caldas Raquel Quintanilla TainãFigueiredo Cardoso Rayner González-Prendes Joan Tibau Marcel Amills Centre for Research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBUniversitat Autònoma de Barcelona08193 BellaterraSpain Animal Breeding and Genetics Program Institute for Research and Technology in Food and Agriculture(IRTA)Torre Marimon08140 Caldes de MontbuiSpain Present address:embrapa Pecuária Sudeste Empresa Brasileira de Pesquisa Agropecuária(EMBRAPA)São CarlosSP 13560-970Brazil Department of Animal Science Universitat de Lleida-Agrotecnio Center25198 LleidaSpain Departament de Ciència Animal i dels Aliments Universitat Autònoma de Barcelona08193 BellaterraBarcelonaSpain

Background:The role of non-coding RNAs in the porcine muscle metabolism is poorly understood,with few studies investigating their expression patterns in response to nutrient ***,we aimed to investigate the changes in microRNAs(miRNAs),long intergenic non-coding RNAs(lincRNAs)and mRNAs muscle expression before and after food ***:We measured the miRNA,lincRNA and mRNA expression levels in the gluteus medius muscle of 12 gilts in a fasting condition(AL-T0)and 24 gilts fed ad libitum during either 5 h.(AL-T1,N=12)or 7 h.(AL-T2,N=12)prior to *** small RNA fraction was extracted from muscle samples retrieved from the 36 gilts and sequenced,whereas lincRNA and mRNA expression data were already *** terms of mean and variance,the expression profiles of miRNAs and lincRNAs in the porcine muscle were quite different than those of *** intake induced the differential expression of 149(AL-T0/AL-T1)and 435(AL-T0/AL-T2)mRNAs,6(AL-T0/AL-T1)and 28(AL-T0/AL-T2)miRNAs and none lincRNAs,while the number of differentially dispersed genes was much *** the set of differentially expressed miRNAs,we identified ssc-miR-148a-3p,ssc-miR-22-3p and ssc-miR-1,which play key roles in the regulation of glucose and lipid ***,co-expression network analyses revealed several miRNAs that putatively interact with mRNAs playing key metabolic roles and that also showed differential expression before and after *** case example was represented by seven miRNAs(ssc-miR-148a-3p,ssc-miR-151-3p,ssc-miR-30a-3p,ssc-miR-30e-3p,ssc-miR-421-5p,ssc-miR-493-5p and ssc-miR-503)which putatively interact with the PDK4 mRNA,one of the master regulators of glucose utilization and fatty acid ***:As a whole,our results evidence that microRNAs are likely to play an important role in the porcine skeletal muscle metabolic adaptation to nutrient availability.

关键词： Co-expression analysis lincRNAs microRNAs Pig Regulatory impact factor Skeletal muscle

Effectiveness of traffic light system on Brazilian consumers perception of food healthfulness

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Food Science and Human Wellness 2019年第4期8卷 368-374页

作者： Mayara Lima Marcela de Alcantara Amauri Rosenthal Rosires Deliza IT/DTA–Federal Rural University of Rio de Janeiro BR 465–Km 07CEP 23.890-000SeropédicaRJBrazil Nutrition and Dietetics Department–Federal Fluminense University Rua Mário Santos Braga30CEPNiteróiRJBrazil PDJ-CNPq/embrapa Agroindústria de Alimentos Av.das Américas29501CEP 23.020-470Rio de JaneiroRJBrazil embrapa Agroindústria de Alimentos Av.das Américas29501CEP 23.020-470Rio de JaneiroRJBrazil

Front-of-package(FOP)nutrition labelling schemes were developed to improve consumer’s comprehension about the food nutrients associated with non-communicable diseases(NCDs).Several FOPs have already been developed,and Brazil is in the process of evaluating a scheme to introduce in the *** aim of this study was to investigate the impact of TLS,the scheme proposed by the food industry,on the product healthfulness perception.A study with 141 participants was carried out.A conjoint task was designed considering three categories and levels:types of dairy products(light yogurt,prato cheese,and chocolate flavoured milk),Traffic Light System(yes ***)and brand(well-known ***).The effect of TLS on perceived healthfulness was evaluated using a 9-point scales(1:not healthy;9:very healthy).Results showed that the inclusion of TLS did not influence the perceived healthfulness of the products by consumers,even among consumers with higher interest in healthy *** findings suggest that the proposal supported by the food industry does not seem to be the most appropriate,being recommended the development of further studies to compare the efficacy of TLS and other FOP schemes.

关键词： Front of pack Traffic light system Healthfulness perception Food products

Mixed-Species Allometric Equations to Quantify Stem Volume and Tree Biomass in Dry Afromontane Forest of Ethiopia

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Open Journal of Forestry 2022年第3期12卷 263-296页

作者： Mindaye Teshome Carlos Moreira Miquelino Eleto Torres Gudeta Weldesemayat Sileshi Patricia Povoa de Mattos Evaldo Muñoz Braz Hailemariam Temesgen Samuel José Silva Soares da Rocha Mehari Alebachew Departamento de Engenharia Florestal Universidade Federal de Vi&#231 osa (UFV) Vi&#231 osa MG Brazil Central Ethiopia Environment and Forest Research Centre Addis Ababa Ethiopia University of Kwazulu-Natal Pietermaritzburg South Africa embrapa florestas Colombo PR Brazil Department of Forest Engineering Resources and Management College of Forestry Oregon State University Corvallis USA Departamento de Ciências Florestais Universidade Federal de Lavras (UFLA) Lavras MG Brazil

Volume and biomass equations are essential tools to determine forest productivity and enable forest managers to make informed decisions. However, volume and biomass estimation equations are scarce for Afromontane forests in Africa in general and Ethiopia in particular. This limits our knowledge of the standing volume of wood, biomass, and carbon stock of the forests therein. In this study, we developed a new mixed-species volume and biomass equations for Afromontane forests and compared them with generic pantropical and local models. A total of 193 sampled trees from seven dominant tree species were used to develop the equations. Various volume and biomass equations were fitted using robust linear and nonlinear regression. Model comparison indicated that the best model to estimate stem volume was ln(v)=-9.909+ 0.954*ln(d2h), whereas the best model to estimate biomass was ln(b)=-2.983+ 0.949*ln(ρd2h) . These equations explained over 85% of the variations in the stem volume and biomass measurements. The mean density and basal area of trees in the forest with d ≥ 2 cm was 631.5 stems·ha-1 and 24.4 m2·ha-1. Based on the newly developed equations, the forest has on average 303.0 m3·ha-1 standing volume of wood and 283.8 Mg·ha-1 biomass stock. The newly developed allometric equations derived from this study can be used to accurately determine the stem volume, biomass, and carbon storage in the Afromontane forests in Ethiopia and elsewhere with similar stand characteristics and ecological conditions. By contrast, the generic pan-tropical and other local models appear to provide biased estimates and are not suitable for dry Afromontane forests in Ethiopia. The estimated stem biomass and carbon stock in the Chilimo forest are comparable with the estimates from various tropical forests and woodlands elsewhere in Africa, indicating the importance of dry Afromontane fores

关键词： Carbon Stock Site-Specific Model Robust Regression Natural Forest

Yield and quality of maize following the foliar application of a fertilizer based on the byproduct “shale water”

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Agricultural Sciences 2013年第12期4卷 56-65页

作者： Rafael da Silva Messias Vanessa Galli Manoel Artigas Schirmer Clenio Nailto Pillon Sérgio Delmar dos Anjos e Silva Carlos Augusto Posser Silveira Cesar Valmor Rombaldi embrapa Clima Temperado Pelotas Brazil embrapa Clima Temperado Pelotas Brazil Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto Alegre Brazil embrapa Clima Temperado Pelotas Brazil Faculdade de Agronomia Eliseu Maciel Universidade Federal de Pelotas Pelotas Brazil Faculdade de Agronomia Eliseu Maciel Universidade Federal de Pelotas Pelotas Brazil

The water extracted from the shale rock (shale water) through the pyrolysis process to obtain fuel oil and other products shows a composition based on organic compounds and a wide range of minerals and trace elements with an important role in plant nutrition, suggesting its use as a fertilizer. Thus, the influence of foliar application of shale water (SW), with or without the micronutrients zinc (Zn), manganese (Mn), copper (Cu), boro (B) and molybdenum (Mo), was evaluated regarding yield and quality of maize grains. The yield, the total antioxidant activity, and the content of starch, phenolic compounds and carotenoids were improved in maize grains following the application of three doses of 7 L/ha of SW, which indicates that SW may influence the primary and secondary metabolisms. The application of SW with micronutrients resulted in the increase of grain yield;however, did not result in the improvement of grain quality. The foliar fertilizer formulations also had an influence in the content of minerals and aminoacids of the grain. The results indicate that SW has potential to be used in agriculture to improve yield and quality of maize.

关键词： Maize Shale Water Foliar Application Grain Quality Biofortification

Use of High-Yielding Bioenergy Plant Castor Bean(Ricinus communis L.) as a Potential Phytoremediator for Copper-Contaminated Soils

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Pedosphere 2013年第5期23卷 651-661页

作者： R. ANDREAZZA L. BORTOLON S. PIENIZ F. A. O. CAMARGO Department of Soil Federal University of Rio Grande do Sul Laboratory of Soil Microbiology Center of Engineer Federal University of Pelotas embrapa - Brazilian Agricultural Research Corporation National Research Center of Fisheries Aquaculture and Agricultural Systems Department of Technology and Food Microbiology Federal University of Rio Grande do Sul

Copper （Cu） contamination in the environment has been increased during the years with agricultural and industrial activities. Biotechnological approaches are needed for bioremediation in these areas. The aims of this study were i） to evaluate the phytoreme- diation capacity of the high-yielding bioenergy plant castor bean （Ricinus communis L.） in vineyard soils （Inceptisol and Mollisol） contaminated with Cu and a Cu mining waste; ii） to characterize the castor bean as a Cu phytoremediation plant; and iii） to evaluate the nutrient uptake by castor bean. Castor bean plants cultivated in soil with toxic levels of Cu for 57 d exhibited high phytomass production, a high tolerance index of roots＇ fresh mass and shoots＇ dry mass, a high level of Cu phytoaccumulation in the roots and also, a robust capacity for Cu phytostabilization. Furthermore, castor bean plants did not significantly deplete soil nutrients （such as N, P, and Mg） during cultivation. Plants cultivated in Inceptisol, Mollisol and Cu mining waste exhibited a strong potential for Cu phytoaccumulation, with values of 5900, 3 052 and 2805 g ha-1, respectively. In addition, the castor bean＇s elevated phytomass production and strong growth in Cu-contaminated soils indicated a high level of Cu phytoaccumulation and a potential application in biofuels. These findings indicate that the castor bean is a efficient hyperaccumulator of Cu and a potential candidate plant for the phytoremediation of Cu-contaminated soil.

关键词： biofuels phytoaccumulation phytoremediation phytostabilization

Comparison of grain protein profiles of Brazilian cowpea (Vigna unguiculata) cultivars based on principal component analysis

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Food Production, Processing and Nutrition 2022年第1期4卷 172-182页

作者： Tuany Camila Honaiser Gabriela Barbosa Rossi Maurisrael de Moura Rocha Ana Carolina Maisonnave Arisi Food Science and Technology Department CAL CCA UFSCFederal University of Santa CatarinaRod.Admar GonzagaFlorianópolisSanta Catarina134688034-001Brazil embrapa Meio-Norte Caixa Postal 001TeresinaPiauí64008-780Brazil

This study aims to compare the grain protein profile of four Brazilian cowpea cultivars (BRS Aracê, BRS Itaim, BRS Pajeú, and BRS Xiquexique) by two-dimensional electrophoresis (2-DE) and principal component analysis (PCA). 2-DE efficiently separate cowpea protein profiles, showing high homogeneity among the four cultivars. In addition, the principal component analysis indicated that there is a difference in abundance of proteins among the cultivars. The cultivars BRS Aracê and BRS Xiquexique, both biofortified in iron and zinc, were separated from the cultivars BRS Itaim and BRS Pajeú. These results demonstrate that protein profiles can be used to discriminate cowpea varieties.

关键词： Vigna 2-DE PCA Legumes

Soybean Seed Co-Inoculation with <i>Bradyrhizobium</i>spp. and <i>Azospirillum brasilense</i>: A New Biotechnological Tool to Improve Yield and Sustainability

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American Journal of Plant Sciences 2015年第6期6卷 811-817页

作者： Mariangela Hungria Marco Antonio Nogueira Ricardo Silva Araujo embrapa Soja Londrina Brazil Total Biotecnologia Indústria e Comércio Ltda Curitiba Brazil

Legume nodulation by rhizobia can supply crops with nitrogen and reduce environmental impacts caused by chemical fertilization. The soybean crop in Brazil is an impressive example of how biological N2 fixation can be employed with a plant species of high economic value. However, the development of more productive cultivars, along with the increasing global climatic changes demand agricultural practices to become more productive and yet more environmentally friendly. Plant growth-promoting rhizobacteria (PGPR) are highly beneficial to agriculture worldwide, acting in plant nutrition, protection, and growth stimulation. Azospirillum is, certainly, the most employed PGPR in the world, but little is known about its interaction with rhizobia, when both are applied to legume seeds. We have evaluated the co-inoculation of bradyrhizobia and azospirilla on soybean seeds under different soil and climate conditions in Brazil. Our results demonstrated that co-inoculation is efficient and beneficial to the crop, and promotes yield increases without adding any chemical N fertilizers even in soils where established populations of soybean bradyrhizobia exist. The strategy of co-inoculation thus represents a new biotechnological tool to improve soybean yield without adding any chemical N fertilizers, thus contributing to current practices of sustainability in agriculture.

关键词： Soybean Azospirillum Bradyrhizobium Inoculation Yield

Comparison of Experimental Methods to Assess the Competitive Ability of Weed Species

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American Journal of Plant Sciences 2015年第13期6卷 2185-2196页

作者： Leandro Galon Germani Concenco Gismael Francisco Perin Alexandre Ferreira da Silva Cesar Tiago Forte Felipe de Adelio David Lauri Lourenco Radüz André Luiz Radunz André Andres Siumar Pedro Tironi Samara Emerim Concenco Plant Science Department Federal University of the Southern Border Erechim Brazil Weed Science Embrapa Western Agriculture Dourados Brazil embrapa Maize and Sorghum Sete Lagoas Brazil Weed Science Embrapa Temperate Agriculture Pelotas Brazil Plant Science Department Federal University of the Southern Border Chapecó Brazil Federal Institute of Santa Catarina at Santa Rosa do Su-SC Santa Rosa do Sul Brazil

Competition between crops and weeds interfere on growth and development with harm to one or both, in different ways. To quantify these differences in competition, additive and replacement series study methods are most often used, each with different characteristics and goals. Therefore, we aimed with this work to study inter-specific competition between plants of soybean and corn against the weeds Bidens pilosa (beggartick) and Digitaria insularis (sourgrass), by two experimental methods of study: additive and replacement series (substitutive) experiments. Initial tests were performed prior to the installation of replacement series experiments to describe the intra-specific competition features for all species. For additive experiments these are unnecessary. To compare the methods of study, there were two sets of trials (an additive and a substitutive) installed under greenhouse, during the growing season 2013/14. Variables analyzed were leaf area and dry weight of shoots of crops and weeds, at 50 days after emergence. The results were analyzed according to the methods used, these being compared. It was found that the additive method supplied results most prone to be applied in the field, while the replacement series (substitutive) method was more appropriate for studies that aimed to provide detailed scientific information regarding the proportions of plants and coefficients describing the behavior of the species community.

关键词： Interference Zea mays Glycine max Weeds

Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development

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Horticulture Research 2016年第1期3卷 124-133页

作者： Danielle Costenaro da Silva Vítor da Silveira Falavigna Marianna Fasoli Vanessa Buffon Diogo Denardi Porto Georgios Joannis Pappas Jr Mario Pezzotti Giancarlo Pasquali Luís Fernando Revers Graduate Program in Cell and Molecular Biology Center for BiotechnologyUniversidade Federal do Rio Grande do SulPorto Alegre91501-970RSBrazil Dipartimento di Biotecnologie Universitàdegli Studi di VeronaVerona37134Italy embrapa Uva e Vinho Bento Gonçalves95701-008RSBrazil embrapa Semiárido Petrolina56302-970PEBrazil Department of Cell Biology Universidade de BrasíliaBrasília70910-900DFBrazil Syngenta Seeds Ltda UberlândiaMG38407-049Brazil Modesto CA 95354USA

The Dof(DNA-binding with one finger)protein family spans a group of plant transcription factors involved in the regulation of several functions,such as plant responses to stress,hormones and light,phytochrome signaling and seed *** we describe the Dof-like gene family in grapevine(Vitis vinifera L.),which consists of 25 genes coding for *** extensive in silico characterization of the VviDofL gene family was ***,the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray(cv Corvina)and real-time PCR(cv Pinot Noir)*** phylogenetic analysis comparing grapevine sequences with those of Arabidopsis,tomato,poplar and already described Dof genes in other species allowed us to identify several duplicated *** diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological ***,distinct expression patterns were identified between samples analyzed,corroborating such *** expression results indicate that several VviDofL genes perform their functional roles mainly during flower,berry and seed development,highlighting their importance for grapevine growth and *** identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.

关键词： involvement organs assessed