检索结果-南通市图书馆

Liposome intracellular delivery of Salvia miltiorrhiza Bge. deprivative DS-201 improves its BK_(Ca) channel-activating and vasorelaxing effects

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2016年第8期61卷 622-631页

作者： Lei Yu Jun Cheng Wen-Jun Huang Xiao-Qiu Tan Liang Mao Zhi-Fei Liu Xiao-Rong Zeng Yan Yang Key laboratory of Medical electrophysiology Ministry of Education of China Institute of Cardiovascular ResearchSichuan Medical University

Some drugs exert curative effects intracellu- larly, but their hydrophilic property prohibits the mem- brane-penetrating process and thus limits the curative efficacies, although this property guarantee their solubility in aqueous phase. An example is sodium tanshinone II-A sulfonate （DS-201）, a derivative of Chinese medical herb Danshen （Salvia miltiorrhiza） which is a BKc~ channel opener and a vasodilator. This study established and opti- mized a liposome delivery system which could pack and deliver DS-201 into HEK293 cells transfected with BKca channels, and DS-201 given this way significantly increased the open probability of BKca channel from baseline 0.013 ~ 0.004 to 0.036 -4- 0.011 at -t-40 mV membrane potential （P 〈 0.05） in single-channel attached study, and also increased the current density from baseline 23.2 ＋ 4.4 to 66.0 4- 15.2 pA/pF at ＋40 mV membrane potential （P 〈 0.05）, compared with the direct extracellular administration of this drug. Moreover, showing a ~ 60 % inhibition of the PE or PGF2a induced vascular constric- tion, the DS-201 liposomes did posses significantly enhanced vasorelaxant effect on rat mesenteric artery, compared with 20 % inhibition of the directly administration of this drug （P 〈 0.05）, suggesting that DS-201 delivered by liposomes significantly improved the drug＇s vasorelaxing effect. Taken together, the optimized DS-201 liposomes in our study successfully delivered DS-201 into cells and thus significantly activated BKca channels to reverse the contraction induced by PE and PGF2a, attesting the enhanced bioavailability.

关键词： Drug delivery system - Liposome Sodium tanshinone II-A sulfonate BKca channelVasodilatation

Facet-dependent antibacterial activity of Au nanocrystals

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2020年第12期31卷 3183-3189页

作者： Youkun Zheng Hui Jiang Xuemei Wang State Key laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Drug Discovery Research Center Key Laboratory of Medical Electrophysiology of Ministry of EducationSouthwest Medical UniversityLuzhou 646000China

Engineered nanomaterials have attracted significantly attention as one of the most promising antimicrobial agents for against multidrug resistant *** toxicological responses of nano mate rials are closely related to their physicochemical properties,and establishment of a structure-activity relationship for nanomaterials at the nano-bio interface is of great significance for deep understanding antibacterial toxicity mechanisms of nanomaterials and designing safer antibacterial *** this study,the antibacterial behaviors of well-defined crystallographic facets of a series of Au nanocrystals,including{100}-facet cubes,{110}-facet rhombic dodecahedra,{111}-facet octahedra,{221}-facet trisoctahedra and{720}-facet concave cubes,was investigated,using the model bacteria Staphylococcus *** find that Au nanocrystals display substantial facet-dependent antibacterial *** low-index facets of cubes,octahedra,and rhombic dodecahedra show considerable antibacterial activity,whereas the high-index facets of trisoctahedra and concave cubes remained inert under biological *** result is in stark contrast to the previous paradigm that the high-index facets were considered to have higher bioactivity as compared with low-index *** antibacterial mechanism studies have shown that the facet-dependent antibacterial behaviors of Au nanocrystals are mainly caused by differential bacterial membrane damage as well as inhibition of cellular enzymatic activity and energy *** faceted Au nanocrystals are unique in that they do not induce generation of reactive oxygen species,as validated for most antibiotics and antimicrobial *** findings may provide a deeper understanding of facet-dependent toxicological responses and suggest the complexities of the na no material-cell interactions,shedding some light on the development of high performance Au nanomaterials-based antibacterial therapeutics.

关键词： Au nanocrystals Facets Antibacterial activity Structure-activity relationship Toxicological response

Synaptobrevin-2 facilitates the trafficking and function of atrial SK2 channel

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2018年第5期61卷 599-603页

作者： Tao Li Xuehui Fan Yiyan Yu Linlin Chen Wenjun Huang Yan Yang Jimin Cao Xiaorong Zeng Xiaoqiu Tan Key laboratory of Medical electrophysiology Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease/Institute of Cardiovascular Research Southwest Medical University

Dear Editor,Small conductance Ca2＋-activated potassium （SK） channels are widely expressed in various tissues and play a unique role in regulating cell function by integrating cytosolic Ca2＋ levels and membrane potential （Adelman et al., 2012）. Xu et al. first identified SK channels in the heart and found that SK channels, particularly the SK2 channel, are preferentially expressed in human and mouse atrial myocytes but not in ventricular myocytes （Xu et al., 2003）. This suggests that the SK

关键词：隧道 Ca2+

Potential functions of esophageal cancer-related gene-4 in the cardiovascular system

在线全文

学校读者我要写书评

暂无评论

Frontiers of Medicine 2019年第6期13卷 639-645页

作者： Rui Zhou Yuanshu Liu Wenjun Huang Xitong Dan The Key laboratory of Medical electrophysiology Ministry of Education&Medical Electrophysiological Key Laboratory of Sichuan ProvinceCollaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease of Sichuan ProvinceInstitute of Cardiovascular ResearchSouthwest Medical UniversityLuzhou 646000China

Esophageal cancer-related gene-4(Ecrg4)is cloned from the normal epithelium of the *** is constitutively expressed in quiescent epithelial cells and downregulated during tumorigenesis,and Ecrg4 expression levels are inversely correlated with the malignant phenotype of tumor cells,validating that Ecrg4 is a real tumor suppressor *** other tumor suppressor genes that usually encode membrane or intracellular proteins,Ecrg4 encodes a 148-amino acid pre-pro-peptide that is tethered on the cell surface in epithelial cells,specialized epithelial cells,and human leukocytes,where it can be processed tissue dependently into several small peptides upon cell ***4 is expressed in a wide variety of other cells/tissues,including cardiomyocytes and conduction system of the heart,,the glomus cells of the carotid body,adrenal glands,choroid plexus,and leukocytes among others,where it exerts distinct functions,such as promoting/suppressing inflammation,inducing neuron senescence,stimulating the hypothalamus--pituitary--adrenal axis,maintaining the stemness of stem cells,participating in the rhythm and rate control of the heart,and possibly gauging the responsiveness of the cardiovascular system(CVS)to hypoxia,in addition to tumor ***,we briefly review the latest discoveries on Ecrg4 and its underlying molecular mechanisms as a tumor suppressor and focus on the emerging roles of Ecrg4 in the CVS.

关键词： tumor suppressor gene esophageal cancer-related gene-4 cardiovascular disease hypoxia

Catheter ablation of atrial fibrillation in the elderly

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2014年第4期11卷 291-295页

作者： Louiza Lioni Konstantinos P. Letsas Michael Efremidis Konstantinos Vlachos Georgios Giannopoulos Vasileios Kareliotis Spyridon Deftereos Antonios Sideris Second Department of Cardiology Laboratory of Cardiac Electrophysiology Evangelismos General Hospital of Athens Athens 10676 Greece Department of Cardiology 'G. Gennimatas ' General Hospital of Athens Greece

Background Atrial fibrillation （AF） catheter ablation has emerged as a promising treatment strategy for AF, but has not been widely adopted in the elderly population. The present study aimed to determine the safety and efficacy of AF catheter ablation in the elderly popula-tion. Methods and Results The study population consisted of 316 patients with paroxysmal AF who underwent left atrial ablation. Ninety-five patients were≥65 years （48 males, mean age 68.9 ± 3.0 years old） and 221 patients were〈65 years old （130 males, mean age 52.5 ± 10.4 years old）. After a mean follow-up period of 34.0 ± 15.1 months, 55 （57.9%） patients in the elderly group were free from ar-rhythmia recurrence compared with 149 （67.4%） patients in the younger group （P=0.169）. Procedural complications were uncommon in both study groups. In logistic regression analysis, left atrial diameter （P=0.003）, hypertension （P=0.001）, dyslipidemia （P=0.039）, and coronary artery disease （P=0.018） were independent predictors of AF recurrence in the elderly population. Conclusions Catheter ablation of AF is safe and effective in older patients. Invasive strategies should be considered as an alternative choice in symptomatic elderly patients with AF.

关键词： Ablation Atrial fibrillation The elderly

VERAPAMIL AND VENTRICULAR TACHYCARDIAS

在线全文

学校读者我要写书评

暂无评论

中华医学杂志(英文版) 1991年第7期104卷 567-572页

作者：戴有鼎周荣兴刘柱柏邱振中 Cardiac electrophysiology laboratory Department of Medicine University of Hong Kong Hong Kong

In the absence of myocardial ischacmia, ventricular tachycardias occurring in patients with structural heart disease rarely respond to calcium antagonists like verapamil. In contrast, verapamil is markedly effective in the acute management of "supraventricular" tachycardias. Thus, intravenous verapamil has often been used for therapeutic and diagnostic purposes in patients with wide QRS complex tachycardias. This report details two unusual cases of vertricular tachycardia that are verapamil-seusitive. They highlight two major categories of "idiopathic" ventricular tachycardia, namely fascieular tachycardia and catecholamine-mediated ventricular tachycardia. The risks of verapamil administration for wide complex tachycardias are also reviewed.

关键词：

Predictive models for mild cognitive impairment to Alzheimer's disease conversion

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第9期16卷 1766-1767页

作者： Konstantina Skolariki Graciella Muniz Terrera Samuel O.Danso Bioinformatics and Human electrophysiology laboratory Ionian UniversityCorfuGreece Centre for Dementia Prevention University of EdinburghUK Division of Neuroimaging Centre for Clinical Brain Sciences and Edinburgh ImagingUniversity of EdinburghScotlandUK

Alzheimer's disease(AD) is an irreversible and progressive neurodegenerative disease as well as the most common form of dementia. It usually affects the older population, but early onset AD is still possible(Ritchie e... 详细信息

关键词： Alzheimer al. impairment

Percutaneous interventions in elderly patients with atrial fibrillation： leftatrial ablation and left atrial appendage occlusion

在线全文

学校读者我要写书评

暂无评论

Journal of Geriatric Cardiology 2017年第9期14卷 541-546页

作者： Evgeny N Mikhaylov Tamas Szili-Torok Dmitry S Lebedev Arrhythmia Department Almazov National Medical Research Centre Saint-Petersburg Russia Neuromodulation laboratory Almazov National Medical Research Centre Saint-Petersburg Russia electrophysiology Unit Cardiology Department Erasmus Medical Center Rotterdam the Netherlands

Heart rhythm disturbances become of special importancewhen patients reach a senior age. Atrial fibrillation （AF） isthe most prevalent arrhythmia among the old age groups,and its impact on morbidity and mortality becomes of para-mount significance. In this population, AF is responsible forsignificant amount of thromboembolic cerebrovascularevents, especially for disabling and fatal strokes, Follow-ing announcement of the special issue of the Journal of Geri-atric Cardiology devoted to heart rhythm disorders in theelderly, a number of submissions have been received withAF as the major topic of authors＇ researches.

关键词： Atrial fibrillation Ablation Efficacy Left atrial appendage Occlusion Safety The elderly

Screening of the ubiquitin-proteasome system activators for anti-Alzheimer’s disease by the high-content fluorescence imaging system

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2022年第1期20卷 33-42页

作者： WANG Yi-Ling YOU Jing CAO Jing-Jie LI Wei JING Liu-Yang MEI Qi-Bing WU An-Guo Sichuan Key Medical laboratory of New Drug Discovery and Drugability Evaluation Luzhou Key Laboratory of Activity Screening and Druggability Evaluation for Chinese Materia MedicaSchool of PharmacyEducation Ministry Key Laboratory of Medical ElectrophysiologySouthwest Medical UniversityLuzhou 646000China Luzhou New Drug Evaluation and Research Center Luzhou 646000China

Ubiquitin-proteasome system(UPS)plays an important role in neurodegenerative diseases,such as Alzheimer’s disease(AD),Parkinson’s disease(PD),and Huntington’s disease(HD).The discovery of UPS activators for anti-neurodegenerative diseases is becoming increasingly *** this study,we aimed to identify potential UPS activators using the high-throughput screening method with the high-content fluorescence imaging system and validate the neuroprotective effect in the cell models of *** first,stable YFP-CL1 HT22 cells were successfully constructed by transfecting the YFP-CL1 plasmid into HT22 cells,together with G418 *** degradation activity of the test compounds via UPS was monitored by detecting the YFP fluorescence intensity reflected by the ubiquitin-proteasome degradation signal *** employing the high-content fluorescence imaging system,together with stable YFP-CL1 HT22 cells,the UPS activators were successfully screened from our established TCM *** representative images were captured and analyzed,and quantification of the YFP fluorescence intensity was performed by flow ***,the neuroprotective effect of the UPS activators was investigated in pEGFP-N1-APP(APP),pRK5-EGFP-Tau P301L(Tau P301L),or pRK5-EGFP-Tau(Tau)transiently transfected HT22 cells using fluorescence imaging,flow cytometry,and Western *** conclusion,our study established a high-content fluorescence imaging system coupled with stable YFP-CL1 HT22 cells for the highthroughput screening of the UPS *** compounds,namely salvianolic acid A(SAA),salvianolic acid B(SAB),and ellagic acid(EA),were identified to significantly decrease YFP fluorescence intensity,which suggested that these three compounds are UPS *** identified UPS activators were demonstrated to clear AD-related proteins,including APP,Tau,and Tau ***,these findings provide a novel insight into the discovery and development of anti-AD drugs.

关键词： Ubiquitin-proteasome system High-content fluorescence imaging system Traditional Chinese medicine Alzheimer’s disease YFP-CL1 HT22 cells