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Food Science and Human Wellness 2024年第1期13卷 506-516页

作者： Zhongai Chen Hong Chen Huan Du Cong Chen Kaixiang Lu Qiaoli Xue Yongjin Hu College of Food Science and Technology Yunnan Agricultural UniversityKunming 650000China Institute of Food Processing Guizhou Academy of Agricultural SciencesGuiyang 550006China editorial department of journal of yunnan agricultural university Yunnan Agricultural UniversityKunming 650000China

Sanchuan ham is appreciated in yunnan Province,China,for its characteristic flavor and taste,while the microbial community structure and biogenic amines content remain unclear during fermentation processes.In this study,we explored the physicochemical property,biogenic amines concentration and microbial diversity of external and internal Sanchuan ham by high-throughput sequencing during the processing of Sanchuan ham.Results showed that the nitrite remained at a stable level of 0.15 mg/kg which was significantly lower than the national health standard safety level of 20 mg/kg.In addition,compared with fresh hams,the content of total free amino acids in ripe Sanchuan ham has grown 14 folds;sour and bitter were the main tastes of Sanchuan ham.Notably,the concentration of cadaverine was the highest of all biogenic amines during the entire fermentation period.At the bacterial phyla level,Firmicutes and Actinobacteria were the two main phyla,while at the genus level,Staphylococcus was a significant strain throughout the whole fermentation.Moreover,the dry stage has a great impact on the succession change of microbial community structure.Simultaneously,the change trends and composition of bacteria in the interior have slight discrepancies with those of the exterior of Sanchuan ham.

关键词： Sanchuan ham Nitrite cont ent Free amino acids Cadaverine Staphylococcus

Development and Quality Analysis of Malus prunifolia Jam

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Bond-Slip Behavior of Steel Bar and Recycled Steel Fibre-Reinforced Concrete

Science and Technology of Food Industry 2024年第11期45卷 175-186页

作者： Wang, Yuanli Wang, Fei Zhang, Quan Tang, Muguo Tao, Liang Tian, Yang College of Food Science and Technology Yunnan Agricultural University Kunming 650000 China Engineering Research Center of Development Utilization of Food and Drug Homologous Resources Ministry of Education Yunnan Agricultural University Kunming 650000 China yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing Yunnan Agricultural University Kunming 650000 China

In order to develop the nutrition and unique flavor of Malus prunifolia jam, the process optimization and product development were used by Box-Behnken response surface method with the addition of Malus prunifolia pulp, honey pomelo peel pectin, white sugar and concentration time as factors and sensory scores as response values. The sensory indexes, physical and chemical indexes, microbial indexes and quality characteristics of the finished products were measured. The results showed that the optimum processing conditions of the Malus prunifolia jam were 77.7% of the Malus prunifolia pulp, 5.0% of the honey pomelo peel pectin, 17.3% of the white sugar, and the concentration time was 17 min. The results of sensory indexes showed that the Malus prunifolia jam made under the optimized conditions were tasted sweet and sour, and presented bright red orange color. Physicochemical index results showed that pH of Malus prunifolia jam was 4.41, total acid content was 5.14%, total flavone content was 31.40 mg/100 g, total sugar content was 10.26%, soluble solid content was 40.61%, vitamin C content was 7.61 mg/g. The results of microbiological index showed that the total number of bacterial colonies was 50 CFU/g, mold, coliform and Staphylococcus aureus were not detected. The results of quality characteristics showed that the Malus prunifolia jam belonged to pseudoplastic fluid, the storage modulus (G') and loss modulus (G") of Malus prunifolia jam increased with the increase of oscillation frequency, and G'>G", showing the characteristics of elastic viscoelastic body. And the most volatile flavor compounds were α-farnesene, methylheptenone and 2, 4-di-tert-butylphenol. The development of Malus prunifolia jam has a certain significance to enrich the product types of Malus prunifolia, improve the edible convenience of Malus prunifolia and promote the development of the industrial chain of Malus prunifolia. © 2024 editorial department of Science and Technology of Food Science. All rights reserved.

关键词： Physicochemical properties

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journal of Renewable Materials 2024年第1期12卷 167-186页

作者： Ismail Shah Jing Li Nauman Khan Hamad R.Almujibah Muhammad Mudassar Rehman Ali Raza Yun Peng School of Architecture and Civil Engineering Yunnan Agricultural UniversityKunming650000China Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu610041China yunnan International Joint R&D Center of Smart Agriculture and Water Security Yunnan Agricultural UniversityKunming650201China School of Water Conservancy Yunnan Agricultural UniversityKunming650201China School of Civil Engineering Guizhou UniversityGuiyang550025China department of Civil Engineering College of EngineeringTaif UniversityP.O.Box 11099Taif City21974Saudi Arabia School of Civil Engineering Zhengzhou UniversityZhengzhou450001China

Recycled steel fiber reinforced concrete is an innovative construction material that offers exceptional mechanical properties and durability.It is considered a sustainable material due to its low carbon footprint and environmental friendly characteristics.This study examines the key influencing factors that affect the behavior of this material,such as the steel fiber volume ratio,recycled aggregate replacement rate,concrete strength grade,anchorage length,and stirrup constraint.The study investigates the bond failure morphology,bond-slip,and bond strength constitutive relationship of steel fiber recycled concrete.The results show that the addition of steel fibers at 0.5%,1.0%,and 1.5%volume ratios can improve the ultimate bond strength of pull-out specimens by 9.05%,6.94%,and 5.52%,respectively.The replacement rate of recycled aggregate has minimal effect on the typical bond strength of pull-out specimens.However,the ultimate bond strengths of pull-out specimens with concrete strength grades C45 and C60 have improved compared to those with C30 grade.The specimens with longer anchorage lengths exhibit lower ultimate bond strength,with a reduction of 33.19%and 46.37%for anchorage lengths of 5D and 7D,respectively,compared to those without stirrups.Stirrup restraint of 1φ8 and 2φ8 improves the ultimate bond strength by 5.29%and 6.90%,respectively.Steel fibers have a significant effect on the behavior of concrete after it cracks,especially during the stable expansion stage,crack instability expansion stage,and failure stage.

关键词： Recycled coarse aggregates(RCA) steel fiber bonding performance bond-slip environmental challenges

The ABA synthesis enzyme allele OsNCED2^(T)promotes dryland adaptation in upland rice

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The Crop journal 2024年第1期12卷 68-78页

作者： Liyu Huang Yachong Bao Shiwen Qin Min Ning Qinyan Li Qingmao Li Shilai Zhang Guangfu Huang Jing Zhang Wensheng Wang Binying Fu Fengyi Hu Key Laboratory of Biology and Germplasm Innovation of Perennial Rice from Ministry of Agriculture and Rural Affairs School of AgricultureYunnan UniversityKunming 650091YunnanChina National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Upland rice shows dryland adaptation in the form of a deeper and denser root system and greater drought resistance than its counterpart,irrigated rice.Our previous study revealed a difference in the frequency of the OsNCED2 gene between upland and irrigated populations.A nonsynonymous mutation(C to T,from irrigated to upland rice)may have led to functional variation fixed by artificial selection,but the exact biological function in dryland adaptation is unclear.In this study,transgenic and association analysis indicated that the domesticated fixed mutation caused functional variation in OsNCED2,increasing ABA levels,root development,and drought tolerance in upland rice under dryland conditions.OsNCED2-overexpressing rice showed increased reactive oxygen species-scavenging abilities and transcription levels of many genes functioning in stress response and development that may regulate root development and drought tolerance.OsNCED2^(T)-NILs showed a denser root system and drought resistance,promoting the yield of rice under dryland conditions.OsNCED2^(T)may confer dryland adaptation in upland rice and may find use in breeding dryland-adapted,water-saving rice.

关键词： Upland rice Dryland adaptation ABA Root development Drought tolerance

Who could and should give exercise prescription:Physicians,exercise and health scientists,fitness trainers,or ChatGPT?

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journal of Sport and Health Science 2024年第3期13卷 368-372页

作者： Weimo Zhu Wenguang Geng Lingling Huang Xiong Qin Zezhao Chen Hai Yan yunnan Plateau Thermal Health Industry Innovation Research Institute Tengchong 679100China department of Kinesiology&Community Health University of Illinois at Urbana-ChampaignUrbanaIL 61801USA department of Physical Education Nanjing Agricultural UniversityNanjing 210095China School of Sports Engineering Beijing Sport UniversityBeijing 100084China School of Strength and Conditioning Training Beijing Sport UniversityBeijing 100084China

Benefits of exercise for personal health,as well as preventing and treating chronic diseases,have been well documented during the past several decades.1 Exercise prescription is a specialized field that involves assessing an individual’s health status,fitness level,and specific goals to create a tailored exercise program based often on the FITT-VP principle.

关键词： prescription diseases assessing

Haploids can be induced in knockout mutants of OsPLA1,but not OsDMP3 or OsDMP6,in rice

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The Crop journal 2024年第1期12卷 213-221页

作者： Zongkai Liu Yu Zhong Xiaolong Qi Tai An Shuwei Guo Dong Wang Yuwen Wang Bin Feng Zuofeng Zhu Shaojiang Chen Chenxu Liu National Maize Improvement Center Engineering Research Center of Ministry of EducationChina Agricultural UniversityBeijing 100193China National Center for Evaluation of agricultural Wild Plants(Rice) Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China

Doubled haploid(DH)technology is an important tool in crop breeding because it can significantly accelerate the breeding process.ZmPLA1/MATL/NLD and ZmDMP are two key genes controlling haploid induction(HI)in maize,exhibiting a synergistic effect.However,it is unknown whether knock out of ZmDMP orthologs can stimulate HI in rice.In this study,a ZmPLA1 ortholog(OsPLA1)and two ZmDMP orthologs(OsDMP3 and OsDMP6)were identified in rice.All three genes encode plasma membrane-localized proteins and were highly expressed in mature anthers.Knockout of OsPLA1 in both Minghui 63 and Nipponbare resulted in reduced seed setting rate(SSR)and caused HI.The osdmp3,osdmp6 and the double mutant failed to trigger HI independently,nor increased the haploid induction rate(HIR)when combined with ospla1.Repeated pollinations operations of QX654A with the ospla1 mutant significantly improve SSR,while reducing HIR.RNA-seq profiling of mature ospla1 mutant anthers indicated that a large number of differentially expressed genes(DEGs)were enriched in redox homeostasis and lipid metabolic GO terms,plant hormone signal transduction,and MAPK signaling pathways.These findings provide important insights towards construction of an efficient DH breeding technology and study of the molecular mechanism of HI in rice.

关键词： Doubled haploid breeding Haploid induction Mutation anlaysis Rice

Artificial selection of the Green Revolution gene Semidwarf 1 is implicated in upland rice breeding

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journal of Integrative Agriculture 2024年第3期23卷 769-780页

作者： Shuliang Jiao Qinyan Li Fan Zhang Yonghong Tao Yingzhen Yu Fan Yao Qingmao Li Fengyi Hu Liyu Huang Key Laboratory of Biology and Germplasm Innovation of Perennial Rice Ministry of Agriculture and Rural Affairs/School of AgricultureYunnan UniversityKunming 650091China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Wenshan Academy of agricultural Sciences Wenshan 663000China

Semidwarf breeding has boosted crop production and is a well-known outcome from the first Green Revolution. The Green Revolution gene Semidwarf 1(SD1), which modulates gibberellic acid(GA) biosynthesis, plays a principal role in determining rice plant height. Mutations in SD1 reduce rice plant height and promote lodging resistance and fertilizer tolerance to increase grain production. The plant height mediated by SD1 also favors grain yield under certain conditions. However, it is not yet known whether the function of SD1 in upland rice promotes adaptation and grain production. In this study, the plant height and grain yield of irrigated and upland rice were comparatively analyzed under paddy and dryland conditions. In response to dryland environments, rice requires a reduction in plant height to cope with water deficits. Upland rice accessions had greater plant heights than their irrigated counterparts under both paddy and dryland conditions, and appropriately reducing plant height could improve adaptability to dryland environments and maintain high grain yield formation. Moreover, upland rice cultivars with thicker stem diameters had stronger lodging resistance, which addresses the lodging problem. Knockout of SD1 in the upland rice cultivar IRAT104 reduced the plant height and grain yield, demonstrating that the adjustment of plant height mediated by SD1 could increase grain production in dryland fields. In addition, an SD1 genetic diversity analysis verified that haplotype variation causes phenotypic variation in plant height. During the breeding history of rice, SD1 allelic mutations were selected from landraces to improve the grain yield of irrigated rice cultivars, and this selection was accompanied by a reduction in plant height. Thus, five known mutant alleles were analyzed to verify that functional SD1 is required for upland rice production. All these results suggest that SD1 might have undergone artificial positive selection in upland rice, which provides further insights concerning greater plant heigh

关键词： Green Revolution Semidwarf 1 high-yield breeding upland rice plant height

Uphill or downhill?Cropland use change and its drivers from the perspective of slope spectrum

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journal of Mountain Science 2024年第2期21卷 484-499页

作者： PAN Sipei LIANG Jiale CHEN Wanxu PENG Yelin College of Land Management Nanjing Agricultural UniversityNanjing 210095China National&Local Joint Engineering Research Center for Rural Land Resources Use and Consolidation Nanjing 210095China Jiangsu Provincial Key Laboratory of Geographic Information Technology School of Geography and Ocean ScienceNanjing UniversityNanjing 210023China Key Laboratory of Natural Resources Monitoring and Supervision in Southern Hilly Region Ministry of Natural ResourcesChangsha 410118China yunnan Key Laboratory of Plateau Geographic Processes and Environmental Changes Faculty of GeographyYunnan Normal UniversityKunming 650500China Hubei Key Laboratory of Regional Ecology and Environmental Change School of Geography and Information EngineeringChina University of GeosciencesWuhan 430074China

The continuous decrease of low-slope cropland resources caused by construction land crowding poses huge threat to regional sustainable development and food security.Slope spectrum analysis of topographic and geomorphic features is considered as a digital terrain analysis method which reflects the macro-topographic features by using micro-topographic factors.However,pieces of studies have extended the concept of slope spectrum in the field of geoscience to construction land to explore its expansion law,while research on the slope trend of cropland from that perspective remains rare.To address the gap,in virtue of spatial analysis and geographically weighted regression(GWR)model,the cropland use change in the Yangtze River Basin(YRB)from 2000 to 2020 was analyzed and the driving factors were explored from the perspective of slope spectrum.Results showed that the slope spectrum curves of cropland area-frequency in the YRB showed a first upward then a downward trend.The change curve of the slope spectrum of cropland in each province(municipality)exhibited various distribution patterns.Quantitative analysis of morphological parameters of cropland slope spectrum revealed that the further down the YRB,the stronger the flattening characteristics,the more obvious the concentration.The province experienced the greatest downhill cropland climbing(CLC)was Shannxi,while province experienced the highest uphill CLC was Zhejiang.The most common cropland use change type in the YRB was horizontal expansion type.The factors affecting average cropland climbing index(ACCI)were quite stable in different periods,while population density(POP)changed from negative to positive during the study period.This research is of practical significance for the rational utilization of cropland at the watershed scale.

关键词： Cropland climbing Land use change Slope spectrum Driving factors Geographically weighted regression Yangtze River Basin

Stochastic systems with cross-correlated Gaussian white noises

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Chinese Physics B 2010年第11期19卷 510-515页

作者：王成玉高云宋玉敏周鹏杨海 Physics department Yunnan Normal University editorial department Journal of Yunnan Normal University

This paper theoretically investigates three stochastic systems with cross-correlation Gaussian white noises. Both steady state properties of the stochastic nonlinear systems and the nonequflibrium transitions induced by the cross- correlated noises are studied. The stationary solutions of the Fokker-Planck equation for three specific examples are analysed. It is shown explicitly that the cross-correlation of white noises can induce nonequilibrium transitions.

关键词： phase transitions fluctuation phenomenon stochastic processes

Deciphering the chromatin spatial organization landscapes during BMMSC differentiation

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journal of Genetics and Genomics 2023年第4期50卷 264-275页

作者： Zhaowei Teng Yun Zhu Da Lin Qinggang Hao Qiaoning Yue Xiaochao Yu Shuo Sun Lihong Jiang Sheng Lu department of Orthopedics The First People's Hospital of Yunnan ProvinceAffiliated Hospital of Kunming University of Science and TechnologyKunmingYunnan 650032China Key Laboratory of yunnan Provincial Innovative Application of Traditional Chinese Medicine The First People's Hospital of Yunnan ProvinceKunmingYunnan 650032China Clinical Medical Research Center The First People's Hospital of Yunnan ProvinceKunmingYunnan 650032China The Sixth Affiliated Hospital of Kunming Medical university YuxiYunnan 653100China State Key Laboratory of agricultural Microbiology Huazhong Agricultural UniversityWuhanHubei 430070China Center for Life Sciences School of Life SciencesYunnan UniversityKunmingYunnan 650504China

The differentiation imbalance in bone marrow mesenchymal stem cells(BMMSCs)is critical for the development of bone density diseases as the population ages.BMMSCs are precursor cells for osteoblasts and adipocytes;however,the chromatin organization landscapes during BMMSC differentiation remain elusive.In this study,we systematically delineate the four-dimensional genome and dynamic epigenetic atlas of BMMSCs by RNA sequencing,assay for transposase-accessible chromatin sequencing,and highthroughput chromosome conformation capture.The structure analyses reveal 17.5%common and28.5%-30%specific loops among BMMSCs,osteoblasts,and adipocytes.The subsequent correlation of genome-wide association studies and expression quantitative trait locus(e QTL)data with multi-omics analysis reveal 274 genes and 3634 single nucleotide polymorphisms(SNPs)associated with bone degeneration and osteoporosis(OP).We hypothesize that SNP mutations affect transcription factor(TF)binding sites,thereby affecting changes in gene expression.Furthermore,26 motifs,260 TFs,and 291SNPs are identified to affect the e QTL.Among these genes,DAAM2,TIMP2,and TMEM241 are found to be essential for diseases such as bone degeneration and OP and may serve as potential drug targets.

关键词： Bone marrow mesenchymal stem cells Differentiation Hi-C ATAC-Seq Single nucleotide polymorphisms Genome-wide association studies