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Protective Effect of Sweet Potato Peel against Oxidative Stress in Hyperlipidemic Albino Rats

food and Nutrition Sciences 2019年第5期10卷 503-516页

作者： Sami A Althwab Hassan M. Mousa Khaled M El-Zaha A. M. Abdel Zaher Department of food Science and Human Nutrition College of Agricultural & Veterinary Medicine Qassim University Buraidah Qassim KSA food Science Department Faculty of Agriculture Zagazig University Zagazig Egypt College of Science and Arts at Shaqra Shaqra University Shaqra KSA Biochemistry Division Department of Chemistry Faculty of Science Zagazig University Zagazig Egypt

In the present investigation, the valorization of sweet potato (Ipomoea batatas L.) wastes into food application with health benefits was studied. Growing interest in the replacement of synthetic food antioxidants by natural ones has fostered research on vegetable sources and screening of raw materials to identify new antioxidants. Different parts of sweet potato contain various phenolic activities. The sweet potato peel had the highest antioxidant activity as compared to other parts. It was reported that sweet potato contains bioactive compounds such as Flavonoids, Polyphenols, The present study was executed to examine the antioxidant components of sweet potato peel and their addition to fermented cow’s milk to improve lipid profile in rats fed high fat diet. Fermented milk was prepared from cow’s milk fortified with 1% and 2% of sweet potato peels (SPP), before inoculation with starter culture containing (S. thermophilus TH4, L. acidophilus La5 and L. bulgaricus). It was found that addition of SPP had no significant effects on the sensory attributes (appearance, body, texture, and flavor) as compared to the control samples. Increasing the percentage of SPP resulted in a decrease in the viscosity of the fermented milk. When SPP powder was included in the diet and fed to rats, there was a significant decrease in the activities of serum transaminases (ALT and AST). Results also showed that the fermented milk administration to hyperlipidemic/hyper-cholesterolemic rats induced a significant decrease in the total cholesterol, LDL, protein and triglycerides, and a significant increase in HDL concentration. Histopathological examination showed that groups fed with SPP blended fermented milk showed less pathological changes compared to the positive control group. The main results indicated that there is a high potential for sweet potato peel extract to be utilized as antioxidant in food systems due to its high phenolic contents. It can be concluded that the antioxidant com

关键词： Sweet Potato Peel Total Phenolic Organoleptic Properties Oxidative Stress Fermented Milk

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Rice bran hydrolysates induce immunomodulatory effects by suppression of chemotaxis, and modulation of cytokine release and cell-mediated cytotoxicity

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Asian Pacific Journal of Tropical Biomedicine 2020年第10期10卷 470-478页

作者： Suphanthip Phusrisom Laddawan Senggunprai Auemduan Prawan Sarinya Kongpetch Upa Kukongviriyapan Supawan Thawornchinsombut Ronnachai Changsri Veerapol Kukongviriyapan Department of Pharmacology Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of Physiology Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of food Technology Faculty of TechnologyKhon Kaen UniversityKhon Kaen 40002Thailand Chumphae Rice Research Center Ministry of Agriculture and CooperativesKhon Kaen 40130Thailand

Objective: To evaluate the immunomodulatory effects of rice bran hydrolysates on cultured immune cells and their underlying ***: Rice bran hydrolysates were prepared from pigmented rice(Oryza sativa L.) by hydrothermolysis and protease digestion. Rice bran hydrolysates were assayed for phenolic content and antioxidant activity. Cell proliferation of Jurkat, THP-1 and peripheral blood mononuclear cells(PBMC) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Chemotaxis was evaluated by transwell chamber methods. Immunoadherence of THP-1 was performed on cultured human umbilical vein endothelial cells(HUVEC). Cytokine released from PBMC was measured by ELISA assay kits. Lymphocyte-mediated cytotoxicity was carried out on KKU-452 cells. Proteins associated with immunomodulation were analyzed by Western immunoblotting assay. Results: Rice bran hydrolysates were rich in phenolic compounds, such as ferulic acid, catechin, quercetin, and quercetin glycosides. Rice bran hydrolysates suppressed phytohemagglutinin(PHA)-stimulated proliferation of PBMC and Jurkat cells, chemotaxis of Jurkat and THP-1 cells, and immunoadherence of THP-1 on HUVEC cultured cells. The cellular mechanism of rice bran hydrolysates involved the activation of AMPK as well as suppression of m TOR, NF-κB and VCAM-1. Rice bran hydrolysates potentiated PBMC on the PHA-stimulated release of IL-2, TNF-α, and IL-4, and enhanced PHA-induced non-MHC-restricted cytotoxicity on KKU-452 cancer cells. Conclusions: The immunomodulatory effect of phytochemicals derived from rice bran hydrolysates suggests its therapeutic potential for further investigation.

关键词： cytotoxicity immuno lysates

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Ethanol extracts of Hizikia fusiforme induce apoptosis in human prostate cancer PC3 cells via modulating a ROS-dependent pathway

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Asian Pacific Journal of Tropical Biomedicine 2020年第2期10卷 78-86页

作者： Eun Ok Choi Hyesook Lee Cheol Park Gi-Young Kim Hee-Jae Cha Suhkmann Kim Heui-Soo Kim You-Jin Jeon Hye Jin Hwang Yung Hyun Choi Anti-Aging Research Center Dong-eui UniversityBusan 47340Republic of Korea Department of Biochemistry College of Korean MedicineDong-eui UniversityBusan 47227Republic of Korea Department of Molecular Biology College of Natural SciencesDong-eui UniversityBusan 47340Republic of Korea laboratory of Immunobiology Department of Marine Life SciencesJeju National UniversityJeju 63243Republic of Korea Department of Parasitology and Genetics Kosin University College of MedicineBusan 49267Republic of Korea Department of Chemistry College of Natural SciencesCenter for Proteome Biophysics and Chemistry Institute for Functional MaterialsPusan National UniversityBusan 46241Republic of Korea Department of Biological Sciences College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Department of food and Nutrition College of NursingHealthcare Sciences&Human EcologyDong-eui UniversityBusan 47340Republic of Korea

Objective:To investigate whether ethanol extracts of Hizikia fusiforme could induce apoptosis in human prostate cancer PC3 ***:Cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium *** and mitochondrial membrane potential(MMP)were measured using flow cytometry in PC3 *** damage was assessed by nuclear staining and DNA fragmentation *** of apoptosis-associated proteins were determined by Western blotting *** of caspase-3,-8,and-9 were determined by colorimetric ***,intracellular reactive oxygen species(ROS)generation was detected using a flow cytometer and fluorescence ***:Treatment of PC3 cells with ethanol extracts of Hizikia fusiforme inhibited proliferation,which was associated with induction of apoptosis,and accompanied by increased expression of Fas,Fas-ligand(Fas L),Bax and t Bid,and decreased expression of *** addition,ethanol extracts of Hizikia fusiforme reduced c-Flip expression and activated caspase-8,-9 and-3,resulting in an increase in poly(ADP-ribose)polymerase(PARP)***,in the presence of a pan-caspase inhibitor,ethanol extracts of Hizikia fusiforme-mediated growth inhibition and apoptosis were significantly *** extracts of Hizikia fusiforme also destroyed the integrity of mitochondria due to the loss of MMP,leading to cytosolic release of cytochrome ***,the levels of ROS were markedly increased by treatment with ethanol extracts of Hizikia fusiforme,which was significantly suppressed by the ROS scavenger *** investigation of whether ethanol extracts of Hizikia fusiforme-induced apoptosis was related to the generation of ROS was conducted and the results showed that N-acetyl-L-cysteine fully blocked ethanol extracts of Hizikia fusiforme-induced apoptotic events including loss of MMP,activation of caspase-3,the cytosolic release of cytochrome c and ***:E

关键词： Hizikia fusiforme Apoptosis Prostate cancer cells Caspase Reactive oxygen species

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Induction of NAFLD with Increased Risk of Obesity and Chronic diseases in Developed Countries

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Open Journal of Endocrine and Metabolic diseases 2014年第4期4卷 90-110页

作者： Ian James Martins 1Centre of Excellence in Alzheimer’s disease Research and Care School of Medical Sciences Edith Cowan University Joondalup Australia 2School of Psychiatry and Clinical Neurosciences The University of Western Australia Nedlands Australia 3McCusker Alzheimer’s Research Foundation Hollywood Medical Centre Nedlands Australia

The susceptibility of individuals to obesity has been reported in many developed countries with predisposition of humans to obesity associated with high calorie diets and unhealthy lifestyles. Obesity may closely be involved in cell suicide in various organ diseases with the importance of accelerated aging that requires early intervention with drug therapy to prevent diseases such as non alcoholic fatty liver disease (NAFLD) that has increased in children and reached to approx. 40% of the global population. Obesity is induced by various diets and lifestyle factors such as stress, anxiety and depression which are important to consider with the global increase in obesity and are possibly linked to the rise in individuals with brain disorders that involve neurodegeneration. Xenobiotics such as the endocrine disruptor chemicals that have increased in the environment in various developed countries lead to various chronic endocrine diseases as populations divert towards unhealthy diets and lifestyles with induction of NAFLD and obesity. The amount and nature of food intake that improves and increases liver lipid and xenobiotic metabolism in obese individuals have become important to decrease the risk for increased adiposity in man. High fibre or protein diets that contain leucine may improve liver glucose, lipid and xenobiotic metabolism and require further investigation with xenobiotics such as endocrine disruptors involved in appetite dysregulation and metabolic disorders in developed countries. The use of anti-obese drugs that reduce food intake and improve hypercholesterolemia and cardiovascular disease has been assessed in obesity with drug therapy closely involved either in the prevention or induction of NAFLD and obesity in man.

关键词： Global Induction Obesity Nutrition Xenobiotic

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HYPOGLYCEMIC ACTIVITY OF FERULIC ACID AND RELATED COMPOUNDS FROM RICE BRAN IN DIABETIC MICE

HYPOGLYCEMIC ACTIVITY OF FERULIC ACID AND RELATED COMPOUNDS ...

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The Second International Symposium on Natural Antioxidants: Molecular Mechanisms and Health Effects

作者： H.Morishita M.Ohnishi T.Tsuno A.Hosodai E.Nomurai H.Taniguchi Faculty of Education Wakayama University Department of Pharmaceutical Science Kansai Shinkyu Medical College Tsuno food Industrial Co. Industrial Technology Center of Wakayama Prefecture Industrial Technology Center of Wakayama Prefecture Industrial Technology Center of Wakayama Prefecture

Ferulic acid is a ubiquitous plant constituent that arises from the metabolism of phenylalanine and tyrosine. During the investigation of antioxidant activity of ferulic acid and related compounds in vivo, we found th... 详细信息

关键词： STZ HYPOGLYCEMIC ACTIVITY OF FERULIC ACID AND RELATED COMPOUNDS FROM RICE BRAN IN DIABETIC MICE

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High-Coverage Quantitative Metabolomics Requires Spot-Urine and Spot-Feces Sample Collection in Mouse Model Studies

High-Coverage Quantitative Metabolomics Requires Spot-Urine ...

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2018年中国质谱学术大会（CMSC2018）

作者： Deying Chen Jiong Yu Zhehua Zhang Lanjuan Li Liang Li State Key laboratory and Collaborative Innovation Center for Diagnosis and Treatment of Infectious diseases the First Affiliated HospitalCollege of MedicineZhejiang University Department of Chemistry University of Alberta

Compared to conventional MS and NMR techniques, high-performance chemical isotope labeling(CIL) LC-MS can perform more accurate relative quantification of many more metabolites in comparative samples in metabolomics. While high-coverage quantitative metabolomics offers distinct advantages in many areas of applications such as disease biomarker discovery, it requires a much more stringent control in any pre-analytical process that may introduce a greater error than that of the analytical process itself. In this study, we report our investigation of the effects of mouse urine and fecal sample collection on CIL LC-MS metabolome analysis and a robust workflow of collecting urine and feces that can be used in metabolomics studies of mouse models. We examined and compared the methods of using metabolic cage collection and spot collection of urine and feces in a mouse model of chemical-induced liver disease. C-/C-dansylation LC-MS was used for quantitative profiling of the amine-/phenol-submetabolome changes, allowing the detection of 5075, 5596, 4105, 4674 peak pairs or metabolites from spot urine, spot feces, metabolite-cage-collected period urine and period feces, respectively. It was found that cage-collected samples could be contaminated with food and other sources to the extent that metabolomic comparison of different groups could be seriously compromised. With care and practice, spot urine and spot feces could be readily collected without contamination. We demonstrated the application of this method for examining quantitative changes of metabolomic profiles associated with carbon tetrachloride treated mice and their controls.

关键词： Quantitative Metabolomics Chemical Isotope Labeling Liquid chromatography mass spectrometry Sample collecting

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