检索结果-南通市图书馆

Lipid phosphorylation by a diacylglycerol kinase suppresses ABA biosynthesis to regulate plant stress responses

Molecular plant 2024年第2期17卷 342-358页

作者： Jianwu Li Shuaibing Yao Sang-Chul Kim Xuemin Wang Department of Biology University of Missouri-St.LouisSt.LouisMO 63121USA donald danforth plant science center St.LouisMO 63132USA

Lipid phosphorylation by diacylglycerol kinase(DGK)that produces phosphatidic acid(PA)plays important roles in various biological processes,including stress responses,but the underlying mechanisms remain elusive.Here,we show that DGK5 and its lipid product PA suppress ABA biosynthesis by interacting withABA-DEFICIENT2(ABA2),a key ABA biosynthesis enzyme,to negatively modulate plant responseto abiotic stress tested in Arabidopsis thaliana.Loss of DGK5 function rendered plants less damaged,whereas overexpression(OE)of DGK5 enhanced plant damage to water and salt stress.The dgk5 mutant plants exhibited decreased total cellular and nuclear levels of PA with increased levels of diacylglycerol,whereas DGK5-OE plants displayed the opposite effect.Interestingly,we found that both DGK5 and PA bind to the ABA-synthesizing enzyme ABA2 and suppress its enzymatic activity.Consistently,the dgk5 mutant plants exhibited increased levels of ABA,while DGK5-OE plants showed reduced ABA levels.In addition,we showed that both DGK5 and ABA2 are detected in and outside the nuclei,and loss of DGK5 function decreased the nuclear association of ABA2.We found that both DGK5 activity and PA promote nuclear association of ABA2.Taken together,these results indicate that both DGK5 and PA interact with ABA2 to inhibit its enzymatic activity and promote its nuclear sequestration,thereby sup-pressing ABA production in response to abiotic stress.Our study reveals a sophisticated mechanism by which DGK5 and PA regulate plant stress responses.

关键词： diacylglycerol kinase phosphatidic acid diacylglycerol lipid signaling stress responses lipid-protein binding protein-protein interaction Arabidopsis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

How can we harness quantitative genetic variation in crop root systems for agricultural improvement?

引用

Journal of Integrative plant Biology 2016年第3期58卷 213-225页

作者： Christopher N.Topp Adam L.Bray Nathanael A.Ellis Zhengbin Liu donald danforth plant science center Saint LouisMissouri 63132USA

Root systems are a black box obscuring a comprehensive understanding of plant function,from the ecosystem scale down to the individual. In particular,a lack of knowledge about the genetic mechanisms and environmental effects that condition root system growth hinders our ability to develop the next generation of crop plants for improved agricultural productivity and sustainability. We discuss how the methods and metrics we use to quantify root systems can affect our ability to understand them,how we can bridge knowledge gaps and accelerate the derivation of structurefunction relationships for roots,and why a detailed mechanistic understanding of root growth and function will be important for future agricultural gains.

关键词： Architecture genetics imaging quantification root

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Versatile Phenotyping System and Analytics Platform Reveals Diverse Temporal Responses to Water Availability in Setaria

引用

Molecular plant 2015年第10期8卷 1520-1535页

作者： Noah Fahlgren Maximilian Feldman Malia A. Gehan Melinda S. Wilson Christine Shyu Douglas W. Bryant Steven T. Hill Colton J. McEntee ankalpi N. Warnasooriya Indrajit Kumar Tracy Ficor Stephanie Turnipseed Kerrigan B. Gilbert Thomas P. Brutnell James C. Carrington Todd C. Mockler Ivan Baxter donald danforth plant science center St. Louis MO 63132 USA USDA-ARS Donald Danforth Plant Science Center St. Louis MO 63132 USA These authors contributed equally to this article.

Phenotyping has become the rate-limiting step in using large-scale genomic data to understand and improve agricultural crops. Here, the Bellwether Phenotyping Platform for controlled-environment plant growth and automated multimodal phenotyping is described. The system has capacity for 1140 plants, which pass daily through stations to record fluorescence, near-infrared, and visible images. plant Computer Vision （plantCV） was developed as open-source, hardware platform-independent software for quantitative image analysis. In a 4-week experiment, wild Setaria viridis and domesticated Setaria italica had fundamentally different temporal responses to water availability. While both lines produced similar levels of biomass under limited water conditions, Setaria viridis maintained the same water-use efficiency under water replete conditions, while Setaria italica shifted to less efficient growth. Overall, the Bellwether Phenotyping Platform and plantCV software detected significant effects of genotype and environment on height, biomass, water-use efficiency, color, plant architecture, and tissue water status traits. All -79 000 images acquired during the course of the experiment are publicly available.

关键词： abiotic/environmental stress water relations bioinformatics development phenotyping

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Suppression of Soybean Oleosin Produces Micro-Oil Bodies that Aggregate into Oil Body/ER Complexes

引用

Molecular plant 2008年第6期1卷 910-924页

作者： Monica A. Schmidt Eliot M. Herman donald danforth plant science center 975 N Warson Rd St Louis MO 63132 USA plant Genetics Research Unit USDA/ARS Donald Danforth Plant Science Center 975 N. Warson Rd St Louis MO 63132 USA

Using RNAi, the seed oil body protein 24-kDa oleosin has been suppressed in transgenic soybeans. The endoplasmic reticulum （ER） forms micro-oil bodies about 50 nm in diameter that coalesce with adjacent oil bodies forming a hierarchy of oil body sizes. The oil bodies in the oleosin knockdown form large oil body-ER complexes with the interior dominated by micro-oil bodies and intermediate-sized oil bodies, while the peripheral areas of the complex are dominated by large oil bodies. The complex merges to form giant oil bodies with onset of seed dormancy that disrupts cell structure. The transcriptome of the oleosin knockdown shows few changes compared to wild-type. Proteomic analysis of the isolated oil bodies of the 24-kDa oleosin knockdown shows the absence of the 24-kDa oleosin and the presence of abundant caleosin and lipoxygenase. The formation of the micro-oil bodies in the oleosin knockdown is interpreted to indicate a function of the oleosin as a surfactant.

关键词： RNAi protein soybean en-doplasmic reticulum

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Interaction of Heterotrimeric G-Protein Components with Receptor-like Kinases in plants： An Alternative to the Established Signaling Paradigm？

引用

Molecular plant 2016年第8期9卷 1093-1095页

作者： Swarup Roy Choudhury Sona Pandey donald danforth plant science center St. Louis MO 63132 USA

Heterotrimeric G proteins comprising Ga, Gβ, and Gγ subunits （hereafter termed G proteins） mediate important signaling pro- cesses in all eukaryotes. As per the established paradigm,

关键词： hereafter Signal guanine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Non-specific phospholipase C4 hydrolyzes phosphosphingolipids and phosphoglycerolipids and promotes rapeseed growth and yield

引用

Journal of Integrative plant Biology 2023年第11期65卷 2421-2436页

作者： Bao Yang Jianwu Li Jiayu Yan Ke Zhang Zhewen Ouyang Yefei Lu Huili Wei Qing Li Xuan Yao Shaoping Lu Yueyun Hong Xuemin Wang Liang Guo National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Department of Biology University of MissouriSt.LouisMO 63121USA donald danforth plant science center St.LouisMO 63132USA Yazhouwan National Laboratory Sanya 572025China

Phosphorus is a major nutrient vital for plant growth and development,with a substantial amount of cellular phosphorus being used for the biosynthesis of membrane phospholipids.Here,we report that NON-SPECIFIC PHOSPHOLIPASE C4(NPC4)in rapeseed(Brassica napus)releases phosphate from phospholipids to promote growth and seed yield,as plants with altered NPC4 levels showed significant changes in seed production under different phosphate conditions.Clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated nuclease 9(Cas9)-mediated knockout of Bna NPC4 led to elevated accumulation of phospholipids and decreased growth,whereas overexpression(OE)of Bna NPC4resulted in lower phospholipid contents and increased plant growth and seed production.We demonstrate that Bna NPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in vitro,and plants with altered Bna NPC4 function displayed changes in their sphingolipid and glycerolipid contents in roots,with a greater change in glycerolipids than sphingolipids in leaves,particularly under phosphate deficiency conditions.In addition,Bna NPC4-OE plants led to the upregulation of genes involved in lipid metabolism,phosphate release,and phosphate transport and an increase in free inorganic phosphate in leaves.These results indicate that Bna NPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in rapeseed to enhance phosphate release from membrane phospholipids and promote growth and seed production.

关键词： inorganic phosphorus non-specific phospholipase C4 phosphate deficiency phospholipids phosphorus use efficiency rapeseed

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genome editing in grass plants

引用

aBIOTECH 2020年第1期1卷 41-57页

作者： Si Nian Char Bing Yang Division of plant sciences University of MissouriColumbiaMO 65211USA donald danforth plant science center St.LouisMO 63132USA

Cereal crops including maize,rice,wheat,sorghum,barley,millet,oats and rye are the major calorie sources in our daily life and also important bioenergy sources of the world.The rapidly advancing and state-of-the-art genome-editing tools such as zinc finger nucleases,TAL effector nucleases,and clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated systems(CRISPR-Cas9-,CRISPR-Cas12a-and CRISPR/Cas-derived base editors)have accelerated the functional genomics and have promising potential for precision breeding of grass crops.With the availability of annotated genomes of the major cereal crops,application of these established genome-editing toolkits to grass plants holds promise to increase the nutritional value and productivity.Furthermore,these easy-to-use and robust genome-editing toolkits have advanced the reverse genetics for discovery of novel gene functions in crop plants.In this review,we document some of important progress in development and utilization of genome-editing tool sets in grass plants.We also highlight present and future uses of genome-editing toolkits that can sustain and improve the quality of cereal grain for food consumption.

关键词： Genome editing Cereal crops ZFNs TALENs CRISPR/Cas9 CRISPR/Cas12a Base editor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Arabidopsis AP2/ERF Transcription Factor RAP2.11 Modulates plant Response to Low-Potassium Conditions

引用

Molecular plant 2012年第5期5卷 1042-1057页

作者： Min Jung Kim Daniel Ruzicka Ryoung Shin Daniel P. Schachtman donald danforth plant science center St Louis MO 63132 USA RIKEN plant science center Yokohama Kanagawa 230-0045 Japan Present address:Monsanto Company 700 Chesterfield Parkway Chesterfield MO 63017 USA

plants respond to low-nutrient conditions through metabolic and morphology changes that increase their ability to survive and grow. The transcription factor RAP2.11 was identified as a component in the response to low potassium through regulation of the high-affinity K＋ uptake transporter AtHAK5 and other components of the low- potassium signal transduction pathway. RAP2.11 was identified through the activation tagging of Arabidopsis lines that contained a luciferase marker driven by the AtHAK5 promoter that is normally only induced by low potassium. This factor bound to a GCC-box of the AtHAK5 promoter in vitro and in vivo. Transcript profiling revealed that a large number of genes were up-regulated in roots by RAP2.11 overexpression. Many regulated genes were identified to be in functional cate- gories that are important in Iow-K＋ signaling. These categories included ethylene signaling, reactive oxygen species pro- duction, and calcium signaling. Promoter regions of the up-regulated genes were enriched in the GCCGGC motif also contained in the AtHAK5 promoter. These results suggest that RAP2.11 regulates AtHAK5 expression under Iow-K＋ con- ditions and also contributes to a coordinated response to low-potassium conditions through the regulation of other genes in the Iow-K＋ signaling cascade.

关键词： potassium deprivation transport transcription factor nutrient sensing signal transduction.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

THF_1 mutations lead to increased basal and wound-induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI_1 signaling in Arabidopsis

引用

Journal of Integrative plant Biology 2014年第9期56卷 916-927页

作者： Yi Gan Hong Li Ye Xie Wenjuan Wu Maoyin Li Xuemin Wang Jirong Huang National Key Laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological Sciencesthe Chinese Academy of Sciences School of Agricultural and Food science Zhejiang A&F University Department of Biology University of Missouri donald danforth plant science center

Mutants defective in chloroplast development or photosynthesis are liable to accumulate higher levels of anthocyanin in photo-oxidative stress.However,regulatory mechanisms of anthocyanin biosynthesis in the mutants remain unclear.Here,we investigated the mechanism by which the deletion of thylakoid formation1（THF1） leads to an increased level of anthocyanin in Arabidopsis thaliana L.Physiological and genetic evidence showed that the increased level of anthocyanin in thf1 is dependent on coronatine-insensitive1（COM）signaling.Our data showed that thf1 had higher levels of basalα-linolenic acid（α-LeA）,and methyl jasmonate（JA）-induced α-LeA and 12-oxophytodienoic acid（OPDA） than the wild type（WT）.Consistently,expression levels of phospholipase genes including pPLAIIα and PLA-Iγ1 were elevated in thf1.Furthermore,inhibition of lipase activity by bromoenol lactone,a specific inhibitor of plant pPLA,led to producing identical levels of anthocyanins in WT and thf1 plants.Interestingly,OPDA biosynthesis was triggered by light illumination in isolated chloroplasts,indicating that new protein import into chloroplasts is not required for OPDA biosynthesis.Thus,we conclude that the elevated anthocyanin accumulation in thf1 is attributed to an increase in JA levels.This JA-mediated signaling to coordinate plant metabolism and growth in stress may be conserved in other photosensitive mutants.

关键词： Anthocyanin Arabidopsis jasmonates photo-oxidative stress retrograde signaling THF1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Statistical Growth Property of plant Root Architectures

引用

plant Phenomics 2020年第1期2卷 367-377页

作者： Sam Sultan Joseph Snider Adam Conn Mao Li Christopher N.Topp Saket Navlakha Cold Spring Harbor Laboratory Simons Center for Quantitative BiologyCold Spring HarborNYUSA University of California San Diego Institute for Neural ComputationLa JollaCAUSA donald danforth plant science center St.LouisMOUSA

Numerous types of biological branching networks,with varying shapes and sizes,are used to acquire and distribute resources.Here,we show that plant root and shoot architectures share a fundamental design property.We studied the spatial density function of plant architectures,which specifies the probability of finding a branch at each location in the 3-dimensional volume occupied by the plant.We analyzed 1645 root architectures from four species and discovered that the spatial density functions of all architectures are population-similar.This means that despite their apparent visual diversity,all of the roots studied share the same basic shape,aside from stretching and compression along orthogonal directions.Moreover,the spatial density of all architectures can be described as variations on a single underlying function:a Gaussian density truncated at a boundary of roughly three standard deviations.Thus,the root density of any architecture requires only four parameters to specify:the total mass of the architecture and the standard deviations of the Gaussian in the threeðx,y,zÞgrowth directions.plant shoot architectures also follow this design form,suggesting that two basic plant transport systems may use similar growth strategies.

关键词： networks plant directions

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：