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Genetic characterization of far eastern species of the genus <i>Crepidostomum</i>(Trematoda: Allocreadiidae) by means of 28S ribosomal DNA sequences

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Advances in Bioscience and Biotechnology 2014年第3期5卷 209-215页

作者： Dmitry M. Atopkin Marina B. Shedko Lab. Parasitology Institute of Biology and Soil Science Far Eastern Branch Russian Academy of Sciences Vladivostok Rus-sia Lab. Parasitology Institute of Biology and Soil Science Far Eastern Branch Russian Academy of Sciences Vladivostok Rus-sia Far Eastern Federal University Vladivostok Russia

Genetic divergence and phylogenetic relationships of four species of the genus Crepidostomum Braun, 1900 sensu Caira, Bogea (2005) were revealed using partial sequences of 28S ribosomal RNA gene. Genetic divergence between C. cf. farionis (Muller, 1784) and C. nemachilus Krotov, 1959 was 3.1%, which corresponds to the mean value of interspecific divergence between Crepidostomum species. These two species, therefore, can be recognized as bonafide species. However, we found no genetic differences between 28S rRNA gene sequences of C. nemachilus and C. cf. metoecus Braun,1900 inspite of considerable morphological and ecological differences. Maximal values of genetic p-distances were revealed between C. cf. auriculatum Wedl, 1857 and C. cf. farionis. Phylogenetic relationships of Crepidostomum spp. for which sequence data are available, along with species in other related genera (Bunodera Railliet, 1896 and Allocreadium Loss, 1900) showed a paraphyly of the genus Crepidostomum. Considerable differentiation of C. cf. auriculatum from other Crepidostomum species was revealed, which may reflect the original description of this species in a separate genus Acrolichanus Ward, 1917. Our results are consistent with the conventional systematics that places the four genera (Crepidostomum, Bunodera, Megalogonia and Allocreadium) within the same family.

关键词： DNA Sequencing Crepidostomum Acrolichanus Trematoda Digenea Phylognetic Relationships

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咖啡酸代谢物的抗肾炎活性分析

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生物工程学报 2008年第12期24卷 2140-2141页

作者： Yulia V. Inyushkina Konstantin V. Kiselev Victor P. Bulgakov Biotechnology Department Institute of Biology and Soil Science Far East Branch of Russian Academy of Sciences Vladivostok 690022 Russia

在研究治疗肾功能紊乱植物的细胞培养时,建立了一个Eritrichium sericeum的E-4愈伤组织株系,发现此株系可产生大量的咖啡酸代谢物、(–)-rabdosiin(1.8%干重)和迷迭香酸(4.6%干重),通过诱导(–)-rabdosiin的含量提高至4.1%(干重)。将E-... 详细信息

在研究治疗肾功能紊乱植物的细胞培养时,建立了一个Eritrichium sericeum的E-4愈伤组织株系,发现此株系可产生大量的咖啡酸代谢物、(–)-rabdosiin(1.8%干重)和迷迭香酸(4.6%干重),通过诱导(–)-rabdosiin的含量提高至4.1%(干重)。将E-4愈伤组织喂服Masugi肾炎大鼠,结果发现,与对照组(未喂服E-4愈伤组织的Masugi肾炎大鼠)相比,处理组(喂服E-4愈伤组织)中的大鼠出现如下症状:尿多、排泄物中肌氨酸酐降低、尿蛋白水平降低;当对照组中所有大鼠都出现疼痛症状时,处理组中仍有约/4的大鼠表现出健康状况良好。以上结果表明,E-4株系具有缓解肾炎症状的潜在功能。此外,利用富含多酚的rolC转基因的细胞株系,研究了愈伤组织中咖啡酸代谢物的诱导合成机制。结果发现,在rloC转基因的***愈伤组织中,咖啡酸代谢物的高产与迷迭香酸生物合成中的关键基因CYP98A3的高表达有关。

关键词：咖啡酸代谢物细胞培养血管球性肾炎 CYP98

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A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia

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Journal of Oceanology and Limnology 2023年第4期41卷 1307-1320页

作者： Qin YANG Puyu GUO Elena Y.ABIDUEVA Elena V.LAVRENTYEVA Irina M.LISEVICH Ilya A.OSTERMAN Petr V.SERGIEV Shaowei LIU Chunmei XUE Chenghang SUN Department of Microbial Chemistry Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Beijing Key Laboratory of Antimicrobial Agents Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China College of Life sciences Jiamusi UniversityJiamusi 154000China institute of General and Experimental Biology Siberian BranchRussian Academy of SciencesUlan-Ude 670047Russia Center of Life sciences Skolkovo Institute of Science and TechnologyMoscow 101135Russia Department of Chemistry A.N.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscow 101135Russia

To explore pharmaceutical actinobacteria from salty environments in Siberia,8 soil samples were collected from different depths of Gudzhirganskoe saline lake,the Republic of Buryatia,***,635 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were obtained by culture-dependent *** predominant genus was Streptomyces(74.5%),followed by Microbacterium(4.9%),Agromyces(3.5%),Nocardiopsis(3.0%),and Kitasatospora(2.5%).Seven strains showed relatively low 16 S rRNA similarities(soil samples collected in the highly alkaline and saline lake Gudzhirganskoe by culture-dependent approach and their capacity to produce antibiotics for the first time,which revealed that it deserves to make more efforts to discover new actinobacterial species and potential new antibiotics from the saline lake in Eastern Siberia.

关键词： Gudzhirganskoe saline lake actinobacteria diversity antimicrobial activities phenazine antibiotic

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The influence of freeze-thaw cycles on the granulometric composition of Moscow morainic clay

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Research in Cold and Arid Regions 2015年第3期7卷 199-205页

作者： Ze Zhang Vadim V.Pendin WenJie Feng ZhongQiong Zhang State Key Laboratory of Frozen soil Engineering Cold and Arid Regions Environmental and Engineering Research InstituteChinese Academy of Science Department of Engineering Geology Russian State Geological Prospecting University

The freeze-thaw cycling process considerably changes the composition, structure, and properties of soils. Since the grain size is the most important factor in determining soil characteristics, our current research primarily aims to investigate dynamic changes of the soil fraction when exposed to freeze-thaw conditions. We observed two series of Moscow morainic clayey specimens （gQⅡm）：（Ⅰ） the original series, and （Ⅱ） the remolded series. We subjected each series of soil specimens to different frequencies of freeze-thaw cycles （3, 6, 20, and 40 cycles）, and we used granulometric tests to analyze both series before and after exposure to freeze-thaw conditions. As a result of our experiments, the granulometric compositions tended to be distributed evenly after 40 freeze-thaw processes （i.e., content of fraction for 0.1-0.05 mm was increased after 40 freeze-thaw cycles） because the division of coarse grains and the aggregation of fine grains were synchronized during the freeze-thaw process. The soil grains in both series changed bi-directionally. In the original series, changes of the sand grains were conjugated with the clay grains, and in the remolded series, changes of the sand grains were conjugated with the silt grains, because potential energy difference caused the division and aggregation processes to relate to the counteraction process. The even distribution of soil grain size indicated the state of equilibrium or balance. The granulometric compositions were altered the most during the sixth freeze-thaw cycle, because the coefficient of the intensity variation of the grain fineness （Kvar） had its maximum value at that time.

关键词： Moscow morainic clay freeze-thaw cycles granulometric composition variability

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Late Palaeogene emplacement and late Neogene-Quaternary exhumation of the Kuril island-arc root (Kunashir island) constrained by multi-method thermochronometry

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Geoscience Frontiers 2016年第2期7卷 211-220页

作者： J.De Grave F.I.Zhimulev S.Glorie G.V.Kuznetsov N.Evans F.Vanhaecke B.McInnes Department of Geology and soil science Mineralogy and Petrology Research Unit Ghent University Ghent Belgium institute of Geology and Mineralogy Siberian Branch Russian Academy of Sciences Novosibirsk Russia Tectonics Resources and Exploration (TraX) Department of Earth Sciences School of Physical Sciences University of Adelaide Adelaide Australia John De Laeter Center for Isotope Research Applied Geology/Applied Physics Curtin University Perth Australia Department of Analytical Chemistry Atomic and Mass Spectrometry Research Unit Ghent University Ghent Belgium

The Kuril islands constitute a volcanic island arc-trench system, stretching from eastern Hokkaido （Japan） to Kamchatka （Russia） along the northwestern Pacific subduction system, The current arc consists of several volcanic islands mainly with Neogene basement and capped by several, predominantly andesitic, active subduction stratovolcanoes, Kunashir Island is the southwestern-most island of the arc, just off the Hokkaido coast and represents the study area in this paper. The island is composed of a Lower Complex of mainly late Miocene to Pliocene volcanic rocks, covered by an Upper Complex of younger （basaltic） andesitic lava flows and tuffs on which currently four active volcanic edifices are built. In the Lower Complex sub-volcanic and deeper-seated intrusives of the so-called Prasolov and dokuchaev magmatic complexes are found, More differentiated, tonalitic-granodioritic rocks were collected from these small intrusive bodies. An early Oligocene zircon LA-ICP-MS U/Pb age of 31 Ma for the Prasolov Complex was obtained, showing that the basement of Kunashir Island is older than previously thought. Thermochronometry （apatite fission-track and U-Th-Sm/He and zircon U-ThJHe analyses） further shows that the magmatic basement of the island was rapidly exhumed in the Pleistocene to present levels in a differential pattern, with He-ages ranging from 1.9 to 0.8 Ma. It is shown that the northern section of the island was hereby exhumed more intensely.

关键词： Kuril island arc Kunashir island Northwest pacific Thermochronometry Zircon U-Pb dating

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Spatial analysis of vegetation cover response to climate trends in Khakassia(South Siberia)

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Journal of Mountain science 2023年第10期20卷 2869-2884页

作者： IM Sergei institute of Natural sciences and Mathematics Khakassian State University named after N.F.KatanovAbakan 655000Russia V.N.Sukachev institute of Forest Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of ScienceKrasnoyarsk 660036Russia institute of Ecology and Geography Siberian Federal UniversityKrasnoyarsk 660041Russia institute of Space Research and High Technologies Reshetnev Siberian State University of Science and TechnologyKrasnoyarsk660014Russia

An analysis of the changes in vegetation cover on the territory of the Republic of Khakassia in 2000–2021 due to climatic trends was carried out based on the MODIS *** changes in vegetation cover were estimated based on trends in Normalized Difference Vegetation Index(NDVI)and Enhanced Vegetation Index(EVI).In general,in the 21st century,an increase in the biomass of vegetation cover is *** trends were observed in 16%–22%of the territory,and negative only in 1%–3%.For about 20%of the analyzed territory,a significant influence of climate on the changes in vegetation cover was *** most pronounced negative impact on vegetation cover was caused by summer air and soil temperatures,spring temperature,and summer winds,and the positive impact was caused by summer precipitation and soil *** response of the vegetation cover to climate was non-uniform concerning the ***,a significant correlation with the amount of precipitation was observed for~20%–35%of vegetation growing below 600 m above sea level and for less than 5%above this *** negative effect of summer temperatures on plants prevailed mainly at an elevation below~1400 m above sea *** climate change is likely to lead to significant degradation of vegetation in the steppe and foreststeppe in Khakassia in the coming decades.

关键词： Khakassia South Siberia Vegetation cover Climate trends Remote sensing MODIS NDVI EVI Relief

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Seasonal variations in temperature sensitivity of soil respiration in a larch forest in the Northern Daxing’an Mountains in Northeast China

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Journal of Forestry Research 2022年第3期33卷 1061-1070页

作者： Lin Yang Qiuliang Zhang Zhongtao Ma Huijun Jin Xiaoli Chang Sergey S.Marchenko Valentin V.Spektor College of Forestry and Da Xing’anling Forest Ecosystem Research Station of Inner Mongolia Autonomous Region Inner Mongolia Agricultural UniversityHohhot010019People’s Republic of China Research-Station of Permafrost Geo-Environment Ministry of EducationInstitute of Cold Regions Science and Engineering and School of Civil EngineeringNortheast Forestry UniversityHarbin150040People’s Republic of China State Key Laboratory of Frozen soils Engineering Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou730000People’s Republic of China Geophysical institute University of Alaska FairbanksFairbanksAK99775USA Laboratory of General Geocryology Melnikov Permafrost InstituteSiberia BranchRussian Academy of SciencesYakutskRussia677010

Temperature sensitivity of respiration of forest soils is important for its responses to climate warming and for the accurate assessment of soil carbon budget. The sensitivity of temperature (T_(i)) to soil respiration rate (R_(s)), and Q_(10) defined by e^(10(lnRs−lna)/Ti) has been used extensively for indicating the sensitivity of soil respiration. The soil respiration under a larch (Larix gmelinii) forest in the northern Daxing’an Mountains, Northeast China was observed in situ from April to September, 2019 using the dynamic chamber method. Air temperatures (T_(air)), soil surface temperatures (T_(0cm)), soil temperatures at depths of 5 and 10 cm (T_(5cm) and T_(10cm), respectively), and soil-surface water vapor concentrations were monitored at the same time. The results show a significant monthly variability in soil respiration rate in the growing season (April–September). The Q_(10) at the surface and at depths of 5 and 10 cm was estimated at 5.6, 6.3, and 7.2, respectively. The Q_(10@10 cm) over the period of surface soil thawing (Q_(10@10 cm, thaw) = 36.89) were significantly higher than that of the growing season (Q_(10@10 cm, growth )= 3.82). Furthermore, the Rs in the early stage of near-surface soil thawing and in the middle of the growing season is more sensitive to changes in soil temperatures. soil temperature is thus the dominant factor for season variations in soil respiration, but rainfall is the main controller for short-term fluctuations in respiration. Thus, the higher sensitivity of soil respiration to temperature (Q_(10)) is found in the middle part of the growing season. The monthly and seasonal Q_(10) values better reflect the responsiveness of soil respiration to changes in hydrometeorology and ground freeze-thaw processes. This study may help assess the stability of the soil carbon pool and strength of carbon fluxes in the larch forested permafrost regions in the northern Daxing’an Mountains.

关键词： soil respiration Dynamic chamber method Ground thawing Major growth period soil temperature

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FRESHWATER MALACOFAUNA OF SAKHALIN ISLAND AND KURIL ARCHIPELAGO （ORIGIN PATTERNS,BIODIVERSITY AND BIOGEOGRAPHY）

FRESHWATER MALACOFAUNA OF SAKHALIN ISLAND AND KURIL ARCHIPEL...

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Ⅸ International Congress on Medical and Applied Malacology

作者： L.A.PROZOROVA V.V.BOGATOV S.K.KHOLIN institute of Biology and soil science Far East Branch russian academy of sciences 159 Pr.100 let VladivostokuVladivostok 690022Russia institute of Biology and soil science Far East Branch russian academy of sciences 159 Pr.100 let VladivostokuVladivostok 690022Russia institute of Biology and soil science Far East Branch russian academy of sciences 159 Pr.100 let VladivostokuVladivostok 690022Russia

＜正＞Based on a long-term study of fauna and taxonomy of freshwater mollusks of the russian Far East a detailed data on specific content and distributional patterns of malacofauna of both insular and adjacent mainlan...

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An improved simple snow-atmosphere-soil transfer model

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science China Earth sciences 2012年第7期55卷 1206-1216页

作者： LIU HuiZhi ZHAI XiaoDong SUN ShuFen FENG JianWu WANG Lei State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry Institute of Atmospheric Physics Chinese Academy of SciencesBeijing 100029China Graduate University of Chinese academy of sciences Beijing 100049China State Key Laboratory of Numerical Modeling for Atmospheric sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China

On the basis of a simple snow-atmosphere-soil transfer (SAST) model previously developed,this paper presents an improved snow-atmosphere-soil transfer (ISAST) model that has a new numerical scheme and an improved method of layering the *** new model takes the snow cover temperature and ice content in the snow cover as prognostic *** approach,which effectively solves the snow cover temperature distribution when the snow cover is melting or freezing,lessens the iteration time and computation time,which is important for GCM *** this model,the snow cover is divided into three layers (ISAST3) or seven layers (ISAST7).The simulation results obtained using the ISAST7 model agree well with observations in terms of snow depth,snow equivalent water and snow cover lifetime at five russian *** new ISAST model has better simulation capacity for snow cover than the previous SAST *** the snow cover is deep,the simulation of the ISAST7 model is better than that of the ISAST3 *** shows that our ISAST model is approximately 20% faster than the SAST model.

关键词： snow-atmosphere-soil transfer model snow cover depth snow equivalent water snow cover lifetime

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Testate amoebae communities from some freshwater and soil habitats in China （Hubei and Shandong Provinces）

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Frontiers of Earth science 2012年第1期6卷 1-9页

作者： Anatoly BOBROV Yuri MAZEI Viktor CHERNYSHOV Yingchun GONG Weisong FENG Department of soil Geography Moscow State University Moscow 119899 Russia Department of Zoology and Ecology Penza State Pedagogical University Penza 440026 Russia Laboratory of Protozoan Taxonomy and Ecology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China

Seventy-eight species and forms of testate amoebae were identified from 29 freshwater and soil habitats in three territories of China （Shandong and Hubei Provinces）. Most abundant species from the genera Plagiopyxis, Centropyxis and Trinema represent the globally-distributed and eurybiont group of testate amoe- bae. The species richness was observed to be the lowest （7-12 species per biotope） in sandy sediments of the Yangtze River, but considerably higher （20-30 taxa） in soil environment. In the range of terrestrial habitats, the most remote communities from Laoshan Mountain in Shandong Province, China manifested the highest difference from others. On the other hand, communities originated in the most distant from industrial center places （Guifeng Mountain in Hubei Province, China） possess the most peculiar species composition including specific Gondwa- nian taxa （e.g. Nebela bigibbosa）. In sum, the results obtained provide the evidence that the community complexity and specificity reduce in the places located within areas that are highly populated and intensively visited by humans.

关键词： testate amoebae soil protists fi'eshwaterprotists China

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