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Plasma von Willebrand factor level as a prognostic indicator of patients with metastatic colorectal carcinoma

World Journal of Gastroenterology 2005年第14期11卷 2166-2170页

作者： Wei-ShuWang Jen-KouLin Tzu-ChenLin Tzeon-JyeChiou Jin-HwangLiu Chueh-ChuanYen Po-MinChen divisionofmedicaloncology DepartmentofMedicineTaipeiVeteransGeneralHospitalandNationalYang-MingUniversitySchoolofMedicineTaipei112TaiwanChina DivisionofColorectalSurgery DepartmentofSurgeryTaipeiVeteransGeneralHospitalandNationalYang-MingUniversitySchoolofMedicineTaipei112TaiwanChina

AIM:To evaluate the correlations of plasma von Willebrand factor (vWF) level with the distant metastasis and prognosis of patients with colorectal cancer. METHODS: A total of 86 patients with historically confirmed metastatic colorectal cancers receiving treatment at Taipei Veterans General Hospital were enrolled. All patients had measurable metastatic lesions and life expectancies of more than 3 mo. Plasma vWF levels were measured by immuno-turbidimetric assay and compared with results from 40 non-metastatic colorectal cancer patients and 22 healthy controls. Patients with metastatic colorectal cancer were divided into two groups according to serum vWF levels and the differences between these two groups were analyzed using X2 test. Data on age, gender, performance status, location of primary tumor, extent of metastasis, site of metastases, histological differentiation, serum CEA and plasma vWF levels were analyzed to determine association with survival. Survival curves were constructed by Kaplan-Meier product limit method and the data was analyzed using log-rank test on a microcomputer. Multivariate analysis using the Cox's proportional hazards regression model was then performed to determine the independent prognostic indicators among all of the possible variables. RESULTS: Colorectal cancer patients were identified as having significantly higher plasma vWF concentrations than healthy controls (P<0.05). Moreover, higher vWF plasma levels were associated with advanced tumor stage (P<0.05) and the presence of multiple metastases (P=0.014). Patients with lower vWF plasma levels (≤160%) survived significantly longer than those with a higher plasma vWF level (log-rank test, P= 0.0043). By multivariate analysis, plasma vWF levels (P<0.001), the extent of metastasis (P= 0.012), and the performance status (P=0.014) were identified as independent prognostic factors. CONCLUSION: Our data indicates that high plasma vWF concentrations correlate with advanced diseases and sign

关键词： von Willebrand factor colorectal carcinoma prognosis

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抗肿瘤细胞多药抗性核酶的合成及其活性的研究

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中华肿瘤杂志 2000年第3期22卷 184-188页

作者：王福生雷周云金磊 Takao Ohunma 解放军第三○二医院传染病研究所生物工程室北京100039 divisionofmedicaloncology MountSinaiSchoolofMedicine

目的　研究抗肿瘤细胞多药抗性 (MDR)核酶的生物学活性及其稳定性。方法　构建表达抗MDR1核酶的逆转录病毒载体 (N2A +tRNAmeti 196MDR1 Rz、N2A +tRNAmeti 196MDR1 sRz、N2A +tRNAmeti 1iMDR1 sRz)和合成了裸核酶cDNA。利用体外转... 详细信息

目的　研究抗肿瘤细胞多药抗性 (MDR)核酶的生物学活性及其稳定性。方法　构建表达抗MDR1核酶的逆转录病毒载体 (N2A +tRNAmeti 196MDR1 Rz、N2A +tRNAmeti 196MDR1 sRz、N2A +tRNAmeti 1iMDR1 sRz)和合成了裸核酶cDNA。利用体外转录反应合成 5种anti MDR1核酶 (tRNA 196MDR1 Rz、tRNA 196MDR1 sRz、tRNA iMDR1 sRz、196MDR1 sRz和 196MDR1 Rz)及相应的底物A和底物B。在无细胞体系中检测了各种核酶的切割活性和它们对核酸酶降解的稳定性。结果　anti MDR1核酶均能有效发挥切割作用。其中嵌合型核酶的活性高于裸核酶 ,而且经过stem Ⅱ碱基修饰的核酶催化活性较高。核酶的稳定性依次是tRNA 196MDR1 sRz(嵌合型 ) >tRNA 196MDR1 Rz =196MDR1 sRz(裸核酶 )。在对照组 ,突变的tRNA mut iMDR1 sRz没有切割活性。结论　核酶分子经过stem

关键词：多药抗药性核酶 mRNA 肿瘤药物疗法

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Copy number changes of target genes in chromosome 3q25.3-qter of esophageal squamous cell carcinoma: TP63is amplified in early carcinogenesis but down-regulated as disease progressed

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World Journal of Gastroenterology 2005年第9期11卷 1267-1272页

作者： Chueh-ChuanYen Yann-JangChen Chin-ChenPan Kai-HsiLu PaulChih-HsuehChen Jiun-YiHsia Jung-TaChen Yu-ChungWu Wen-HuHsu Liang-ShunWang Min-HsiungHuang Biing-ShiungHuang Cheng-PoHu Po-MinChen Chi-HungLin divisionofmedicaloncology DepartmentofMedicineTaipeiVeteransGeneralHospitalandNationalYang-MingUniversitySchoolofMedicineTaipeiTaiwanChina FacultyofLifeScience NationalYang-MingUniversityTaipeiTaiwanChina DepartmentofPathology NationalYang-MingUniversityandTaipeiVeteransGeneralHospitalTaiwanChina InstituteofMicrobiologyandImmunology NationalYang-MingUniversityTaipeiTaiwanChina DivisionofThoracicSurgery DepartmentofSurgeryTaichungVeteransGeneralHospitalTaichungTaiwanChina DepartmentofPathology.TaichungVeteransGeneralHospital andDepartmentofPathologyChung-ShanMedicalUniversityTaichungTaiwanChina DivisionofThoracicSurgery DepartmentofSurgeryTaipeiVeteransGeneralHospitalandNationalYang-MingUniversityTaipeiTaiwanChina InstituteofMicrobiologyandImmunology NationalYang-MingUniversityandDepartmentofMedicalResearchandEducationTaipeiVeteransGeneralHospitalTaipeiTaiwanChina InstituteofMicrobiologyandImmunology andInstituteofBiophotonicsNationalYang-MingUniversityTaipeiTaiwanChina

AIM: By using comparative genomic hybridization, gain of 3q was found in 45-86% cases of esophageal squamous cell carcinoma (EC-SCC). Chromosome 3q25.3-qter is the minimal common region with several oncogenes found within this region. However, amplification patterns of these genes in EC-SCC have never been reported. The possible association of copy number changes of these genes with pathologic characteristics is still not clear. METHODS: Real-time quantitative PCR (Q-PCR) was performed to analyze the copy number changes of 13 candidate genes within this region in 60 primary tumors of EC-SCC, and possible association of copy number changes with pathologic characteristics was analyzed by statistics. Immunohistochemistry (IHC) study was also performed on another set of 111 primary tumors of EC-SCC to verify the association between TP63 expression change and lymph node metastasis status. RESULTS: The average copy numbers (±SE) per haploid genome of individual genes in 60 samples were (from centromere to telomere): SSR3: 4.19 (±0.69); CCNL1: 5.24 (±0.67); SMC4L1: 2.01 (±0.16); EVI1: 2.02 (±0.12); hTERC. 5.28 (±0.54); SKIL 2.71 (±0.14); EIF5A2. 1.95 (±0.12); ECT2: 9.18 (±1.68); PIK3CA: 8.13 (±1.17); EIF4G1: 1.07 (±0.05); 557: 3.07 (±0.25); TP63: 2.51 (±0.22); TFRC. 2.42 (±0.19). Four clusters of amplification were found: SSR3 and CCLN1 at 3q25.31; hTERC and SKIL at 3q26.2; ECT2 and PIK3CA at 3q26.31-q26.32; and 55T, TP63 and TFRC at 3q27.3-q29. Patients with lymph node metastasis had significantly lower copy number of TP63 in the primary tumor than those without lymph node metastasis. IHC study on tissue arrays also showed that patients with lymph node metastasis have significantly lower TP63 staining score in the primary tumor than those without lymph node metastasis. CONCLUSION: This study showed that different amplification patterns were seen among different genes within 3q25.3-qter in EC-SCC, and several novel candidate oncogenes (SSR3, SMC4L1, ECT2, and SST) were ide

关键词： Chromosomal aberration Comparative genomic hybridization Esophageal neoplasm Immunohistochemistry Quantitative real-time PCR Tissue array Tumor protein 63

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A CLONALLY DERIVED CELL LINE,9L-EGFR IS USEFUL FOR THE STUDIES OF CANCER CELLS BEARING EGF RECEPTOR

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中国现代医学杂志 2003年第14期13卷 8-11页

作者： LinQi RajeshAgarwak RanaSingh GailS.Harrisona L.MichaelGlodea DepmtmentofUrology XiangyaHospital.CentralSouthUniversityHunan410008 divisionofmedicaloncology DepartmentofMedicineUniversityofColoradoHealthSciencesCenter4200EastNinthAvenueBoxB171DenverCO.80220USA SchoolofPharmacy UniversityofColoradoHealthSciencesCenter4200EastNinthAvenueBoxB171DenverCO.80220USA Towhomallcorrespondenceshouldbeaddressedat:UniversityofColoradoHealthSciencesCenter 4200EastNinthAvenueBoxB171DenverCO.80220USA

Since the epidermal growth factor receptor (EGFR) is a key regulator in cell signaling pathways of cancer cell. To investigate the mechanism between cancer cells survival and its EGFR expression, drug selection of cancer cells target therapy, we generated a cell line, 9L-EGFR, which stably expressed human EGFR; the parental rat glioma cell line, 9L, does not contain endogenous EGFR message or protein. Our results show that 9L-EGFR cells had high levels of EGFR on their cell surface by using RT-PCR, Western analysis and Flow cytometry analysis. The EGFR transfected into 9L cells was capable of being activated by EGF, in which either phosphorylated (p-EGFR) or total (EGFR) was showed by Western blot. This investigation may contribute to the further studies of cancer cells bearing EGFR.

关键词：表皮生长因子受体 9L-EGFR 癌细胞细胞培养 RT-PCR 克隆技术

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