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Effect of tris-extender supplemented with various concentrations of strawberry (Fragaria spp.) on bull semen preservability

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Asian pacific Journal of Reproduction 2018年第2期7卷 93-96页

作者： El-Sheshtawy RI El-Nattat WS Animal Reproduction and Artificial Insemination Department Veterinary DivisionNational Research CenterDokkiGiza

Objective:To evaluate effect of tris-extender supplemented with various concentrations of strawberry (Fragaria spp.) on bull semen ***: Pooled bull semen were extended with tris-citrate-fructose egg yolk diluent (control, 0% strawberry) and various concentrations of tris strawberry (TSB) (1%-6%) to achieve 60 million motile spermatozoa per milliliter. Extended semen were subjected to semen freezing protocol. Semen assessment including motility, alive%, abnormality%, intact sperm membrane (hypo-osmotic swelling test) and conception rate were carried out for both chilled and frozen semen. Results: Results showed that sperm motility after chilling was enhanced in groups treated with various concentrations of TSB from 1% to 5% and exhibited higher significance (P<0.0001) at 6-day post-chilling. In frozen semen, 3%, 4%, 5% and 6% concentrations gave the best significance (P<0.0001) on sperm motility in comparison with the control. Concentration 1% revealed the highest significance (P<0.0001) on alive% as compared to the control. Hypo-osmotic swelling test was maintained as the control. Concentration 3% gave the lowest significance (P<0.0001) considering abnormality%. The conception rate upon using frozen semen in insemination showed higher conception rate in concentrations of 5% and 6% in ***: It is concluded that 1%-5% concentrations of TSB ameliorate bull semen characteristics after chilling, and 3%-6% concentrations of TSB improve bull semen characteristics after freezing. Higher conception rate exists in 5% and 6% concentration of TSB.

关键词： Cattle Semen Preservation Strawberry

Evaluation of the Pathogenicity of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant in Piglets

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Agricultural Sciences in China 2011年第8期10卷 1280-1291页

作者： WEI Tian-chao TIAN Zhi-jun ZHOU Yan-jun AN Tong-qing JIANG Yi-feng XIAO Yan HU Shou-ping PENG Jin-mei HAO Xiao-fang ZHANG Shan-rui TONG Guang-zhi State Key Laboratory of veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150001P.R.China division of Swine Infectious Diseases Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghai 200241P.R.China College of Animal Science and Technology Guangxi UniversityNanning 530005P.R.China

Since May 2006,a highly pathogenic porcine reproductive and respiratory syndrome virus （HP-PRRSV） variant characterized by 30 amino acids deletion within its NSP2-coding region emerged and caused extensive economic losses to China＇s pig *** investigate the in vivo pathogenicity and immune responses of the newly emerging PRRSV,3 groups of 60-d-old conventional piglets were inoculated intranasally with a representative strain of the HP-PRRSV variant HuN4 with 3 different infection doses （3×103-3×105 TCID50）.The results revealed that the virus variant caused severe disease in piglets and the significant clinical characteristics consisted of persistently high fever （41.0-41.9oC） and high morbidity and mortality （60-100%）,the marked clinical signs of PRRS and severe histopathogenic damages in multiple *** induced rapid and intense humoral immune responses and seroconversion was detected in most infected pigs at 7 d post-infection （DPI）.The virus vigorously replicated in vivo and the highest virus average titer was 9.7 log copies mL-1 serum at 7 *** levels of IFN-g and IL-10 cytokine production in serum in this study were also *** together,our results demonstrated that the HP-PRRSV variant HuN4 strain is highly pathogenic for piglets and suitable to be a reference strain of highly virulent PRRSV for evaluating the efficacy of the new vaccines.

关键词： highly pathogenic porcine reproductive and respiratory syndrome virus pathogenicity immune responses viral load

Bioterrorism Threat:A Review of Microbial Forensics Source-Tracing of Some Bioterrorism Agents

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Journal of Forensic Science and Medicine 2018年第3期4卷 161-166页

作者： Bobmanuel Chimaroke Echeonwu Obinna O.Nwankiti Solomon C.Chollom Kayode A.Olawuyi Department of Histopathology and Cytology Forensic Science UnitFederal College of Veterinary and Medical Laboratory Technology Viral Research division National Veterinary Research InstituteVomPlateau StateNigeria

Global perception and consciousness of the threat of bioterrorism seem to have diminished in recent past following achievements in decades of global fight against deadly infectious diseases such as plague and ***,with recent advancement in biotechnology and the arrival on the scene of amoral and rogue states as well as terrorist groups,there is a justifiably heightened global apprehension that bacteria,viruses,and toxicogenic fungi can be weaponized and used to cause great harm to humans and agricultural *** now have on our hands the dilemma of dual-use *** review brings to the fore an aspect of microbial forensics-bioagent source-tracing(attribution)that is very key in mounting an appropriate response to the evident threat *** article places a little more emphasis on the bioagent Yersinia pestis,and the technique of whole-genome sequence typing adjudged the most effective technique for building databases for bioterrorism-associated agents and public health important *** need for international sharing of data and databases of bio-agents is emphasized,as this would enable global applicability of bio-agent source-tracing in cases ofbioterrorism.

关键词： Bioagent bioterrorism microbial forensics source-tracing whole-genome sequence typing

Cats as a potential source of emerging influenza virus infections

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Virologica Sinica 2015年第3期30卷 221-223页

作者： Taisuke Horimoto Fumihiro Gen Shin Murakami Kiyoko Iwatsuki-Horimoto Kentaro Kato Masaharu Hisasue Masahiro Sakaguchi Chairul A. Nidom Yoshihiro Kawaoka Department of veterinary Microbiology University of Tokyo division of Virology Institute of Medical Science University of Tokyo National Research Center for Protozoan Diseases Obihiro University of Agriculture and Veterinary Medicine Department of Internal Medicine II Azabu University Department of veterinary Microbiology I Azabu University Avian Influenza Research Center Airlangga University

Dear Editor,Historically,the influenza virus has not been regarded as a major pathogen of ***,since 2003,natural infections of domestic cats with highly pathogenic H5N1 avian virus causing fatal cases have been reported(Songserm et al.,2006;Yingst et al.,2006;Klopfleisch et al.,2007).Furthermore,infections of this animal with A(H1N1)pdm09 virus,causing respiratory illness with some fatal cases,have also been reported in various

关键词： pathogenic avian fatal illness causing seasonal serological emerging chicken clade

Impact of silymarin enriched semen extender on bull sperm preservability

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Asian pacific Journal of Reproduction 2017年第2期6卷 81-84页

作者： El-Sheshtawy RI El-Nattat WS Animal Reproduction and AI Dept. Veterinary Research DivisionNational Research CentreDokkiEgypt

Objective:To explore the effect of silymarin on bull spermatozoa during cooling and cryopreservation. Methods: Pooled bull semen were diluted by Tris-Citrate-Fructose egg yolk diluents, purified silymarin powder (obtained from the milk thistle silybum marianum), purchased from Unipharma, Al Obour city, Egypt, was soaked in Tris-citric acid-fructose diluent for 48 h at 10℃ making a stock solution (70 mg/mL), from this stock solution we obtained concentrations of 0.18 mg/mL, 0.36 mg/mL, 0.54 mg/mL, 0.72 mg/mL, 0.90 mg/mL in addition to the control (0.0 mg/mL) reaching a final volume of 5 mL in each tube. Egg yolk was added to each tube to obtain silymarin enriched semen extender (SEE) with 20% egg yolk, cooled slowly up to 5℃ and equilibrated for 4 h. Semen was packed into 0.25 mL polyvinyl French straws (IMV, France). After equilibration periods, the straws were placed horizontally on a rack and frozen in a vapor 4 cm above liquid nitrogen for 10 min and were then dipped in liquid nitrogen. Extended semen was subjected to evaluation (motility, alive %, abnormality %, intact sperm membrane (HOST)% and conception rate) in both chilled and frozen ***: Table 1 revealed that Sperm motility of the concentrations 2, 3 and 4 after 8 d of chilling were significantly (P<0.02) higher than control. Sperm motility of the concentration 2 (45.00%±2.89%) after 9 d of chilling was higher than control and the other concentrations. Addition of SEE in concentration 1 and 2 gave post thawing sperm motility as high as the control (47.50±2.81 and 45.00±2.58, respectively) while other concentration have lower effects on motility as compared to the control. Addition of silymarin improved post thawing alive% and was significantly higher (P<0.0001) than the control. SEE decreased significantly (P<0.0001) the % of post thawing abnormal sperm in concentration 3 and 4 (11.83±0.65 and 16.00±0.58, respectively). SEE improved significantly (P<0.018) the % of post thawing intact spermatozoa

关键词： Cattle Semen Preservation Silymarin

Effect ofDiospyros kakienriched extender on cattle bull sperm parameters and conception rate

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Asian pacific Journal of Reproduction 2017年第3期6卷 128-132页

作者： El-Sheshtawy RI El-Nattat WS Animal Reproduction and AI Department. Veterinary Research DivisionNational Research CentreDokkiEgypt

Objective:To explore the effect of Diospyros kaki on cattle spermatozoa during chilling and ***: Five milliliter of blended Persimmon (Diospyros kaki) flesh was added to 45 mL TCF to obtain 10% stock solution. Kaki enriched extender (KEE) was prepared by adding to TCF in concentrations 0.0/5.0 mL (control, 0%), 0.5/4.5 mL (1%), 1/4 mL (2%), 1.5/3.5 mL (3%), 2.0/3.0 mL (4%), 2.5/2.5 mL (5%), 3.0/2.0 mL (6%), 3.5/1.5 mL (7%), 4.0/1.0 mL (8%), 4.5/0.5 mL (9%) and 5.0/0.0 mL (10%) to obtain a final volume 5 mL in each tube. Whole egg yolk was added to each tube to obtain KEE with 20% egg yolk (KEEY), all tubes were centrifuged to get rid of debris. Semen was added to the supernatants in other tubes. Extended semen was subjected to evaluation (motility, alive sperm and intact sperm membrane (HOST) %) in both chilled and cryopreserved semen. Conception rate was carried ***:Sperm motility was significantly (P<0.0001) kept high after 11 d of chilling with the concentration 1%, 2%, 3%, 4%, 5% as compared to the control (41.67±1.67, 41.67±1.67, 40.00±0.00, 41.67±1.67 and 41.67±1.67, respectively) and also non-significantly kept high at the other concentrations up to 9 d of chilling. Addition of KEE had significantly (P<0.0033) improved post thawing sperm motility % with the concentrations 1, 2, 3, 4, 5 and 6% as compared to the control (51.67±5.27, 55.00±3.16, 48.33±1.05, 45.00±3.96, 57.00±2.50, 55.00±5.00 and 43.33±5.11 respectively).While the other concentrations exhibit no effect. Addition of KEE maintained alive sperm%, abnormalities% and % of intact spermatozoa membranes (HOST %) as good as the control with all concentrations of kaki used in our study. The conception rate upon using frozen semen in insemination showed higher conception rate in concentrations of 2%, 4% and 6 % KEE in ***: It could be concluded that some concentrations ofDiospyros kaki improved bull semen quality post-chilling and post-freezing.

关键词： Cattle Semen Preservation Diospyros kaki

Preservability of bull spermatozoa in Tris-egg yolk extender enriched with different concentrations of butylated hydroxytoluene

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Asian pacific Journal of Reproduction 2017年第2期6卷 77-80页

作者： El-Sheshtawy RI El-Nattat WS Gamal A.El-Sisy Animal Reproduction and AI Dept. Veterinary Research DivisionNational Research CentreDokkiEgypt

Objective:To explore the effect of BHT on cattle spermatozoa during cooling and ***: Pooled bull semen were diluted by Tris-Citrate-Fructose egg yolk (TCFY) diluent considered as control (0 BHT) and different concentrations of BHT (1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 mM were prepared in ethanol in prewarmed (37℃) test tubes. The ethanol was allowed to evaporate so that, a thin crystallized layer of BHT was deposited on the inner surface of the tubes. Then extended semen was added into the tubes and incubated at 37℃ for 5 min to allow uptake of BHT by spermatozoa. The tubes were cooled slowly (approximately for 2 h) up to 5℃ and equilibrated for 4 h. After equilibration, semen freezing process was carried out. Extended semen was subjected to evaluation (motility, alive sperm, intact sperm membrane (HOST) % and acrosome integrity) in both cooled and cryopreserved semen. Results:The result revealed that sperm motility of post-cooled spermatozoa improved (P<0.05) by the use of BHT concentrations (1, 2 and 3 mM) in Tris semen extender if compared to the control (85.00±1.09), (83.33± 0.63), (81.67± 0.63) and (78.33± 0.63), respectively. Alive sperm percent was significantly higher in all concentrations of BHT. Sperm abnormalities percent were significantly lower in concentrations of BHT 1 and 2 (11.2±0.2), (11.8±0.2)and (13.4±0.4), respectively. Sperm membrane integrity were significantly higher in BHT concentrations (1, 2, 3, 4 and 5 mM). It is exhibited that improved sperm motility in post-thawed frozen semen in the concentrations of BHT (1, 2, 3 and 4 mM) if compared to the control. The sperm membrane integrity were significantly improved at all concentrations of BHT. Acrosome integrity was significantly higher at BHT concentration 1 mM (81.80±0.57) and (76.00±2.05), ***: It could be concluded that some concentrations of BHT improved bull semen quality post-cooling and post-freezing.

关键词： Cattle Semen Preservation BHT

Association of sexually transmitted infection with semen quality in men from couples with primary and secondary infertility

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Asian Journal of Andrology 2022年第3期24卷 317-322页

作者： Shun Bai Yuan Li Mei-Hong Hu Li Wu Li-Jun Shui Xiao-Han Wang Yi-Xun Liu Qiu-Ling Yue Li-Na Yu Kai-Qiang Fu Xian-Hong Tong Xue-Chun Hu Bo Xu Reproductive and Genetic Hospital The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Dermatology Department The Fifth People’s Hospital of Hainan ProvinceHaikou 570100China Provincial Hospital Affiliated to Anhui Medical University Hefei 230001China Department of Urology The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Reproductive Medicine Center The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China College of veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China

This study aims to compare the prevalence of sexually transmitted infections(STIs)with semen quality in men from couples with primary and secondary *** samples were collected from 133 men who requested fertility *** tract infection with Ureaplasma spp.(UU),Mycoplasma hominis(MH),Mycoplasma genitalium(MG),Chlamydia trachomatis(CT),Neisseria gonorrhoeae(NG),and herpes simplex virus-2(HSV-2)was assessed by PCR-based diagnostic *** all patients,the prevalence of STIs was higher in men from couples with primary infertility than that in men from couples with secondary infertility(39.7%vs 21.7%,P=0.03).The prevalence of UU was 28.8%and 13.3%in men from couples with primary and secondary infertility,*** from couples with primary infertility were more likely to be positive for UU than men from couples with secondary infertility(P=0.04).Regarding the UU subtype,the prevalence of Ureaplasma urealyticum(Uuu)and Ureaplasma parvum(Uup;including Uup1,Uup3,Uup6,and Uup14)did not differ between the two *** associations between the prevalence rates of MH,MG,and CT were found in men from either infertility group.A lower sperm concentration was associated with STI pathogen positivity in men with primary infertility according to the crude model(P=0.04).The crude and adjusted models showed that semen volume(both P=0.03)and semen leukocyte count(both P=0.02)were independently associated with secondary *** findings suggest the importance of classifying the type of infertility during routine diagnosis of seminal tract infections.

关键词： primary infertility secondary infertility semen parameters sexually transmitted infections

High-throughput screening of novel TFEB agonists in protecting against acetaminopheninduced liver injury in mice

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Acta Pharmaceutica Sinica B 2024年第1期14卷 190-206页

作者： Xiaojuan Chao Mengwei Niu Shaogui Wang Xiaowen Ma Xiao Yang Hua Sun Xujia Hu Hua Wang Li Zhang Ruili Huang Menghang Xia Andrea Ballabio Hartmut Jaeschke Hong-Min Ni Wen-Xing Ding Department of Pharmacology Toxicology and TherapeuticsUniversity of Kansas Medical CenterKansas CityKS 66160USA NMPA Key Laboratory for Research and Evaluation of Drug Metabolism&Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Department of Oncology the First Affiliated Hospital of Anhui Medical UniversityAnhui Medical UniversityHefei 230032China National Center for Advancing Translational Sciences National Institutes of HealthBethesdaMD 20892USA Telethon Institute of Genetics and Medicine TIGEMPozzuoliNaples 80131Italy Medical Genetics Department of Translational MedicineFederico II UniversityNaples 80131Italy Department of Molecular and Human Genetics Baylor College of MedicineHoustonTX 77030USA Department of Internal Medicine Division of GastroenterologyHepatology&MotilityUniversity of Kansas Medical CenterKansas CityKS 66160USA

Macroautophagy(referred to as autophagy hereafter)is a major intracellular lysosomal degradation pathway that is responsible for the degradation of misfolded/damaged proteins and *** studies showed that autophagy protects against acetaminophen(APAP)-induced injury(AILI)via selective removal of damaged mitochondria and APAP protein *** lysosome is a critical organelle sitting at the end stage of autophagy for autophagic degradation via fusion with *** the present study,we showed that transcription factor EB(TFEB),a master transcription factor for lysosomal biogenesis,was impaired by APAP resulting in decreased lysosomal biogenesis in mouse *** loss-of and gain-of function of hepatic TFEB exacerbated or protected against AILI,***,overexpression of TFEB increased clearance of APAP protein adducts and mitochondria biogenesis as well as SQSTM1/p62-dependent non-canonical nuclear factor erythroid 2-related factor 2(NRF2)activation to protect against *** also performed an unbiased cell-based imaging high-throughput chemical screening on TFEB and identified a group of TFEB *** these agonists,salinomycin,an anticoccidial and antibacterial agent,activated TFEB and protected against AILI in *** conclusion,genetic and pharmacological activating TFEB may be a promising approach for protecting against AILI.

关键词： Autophagy DILI Drug screening Hepatotoxicity Lysosome Mitochondria Mitophagy NRF2