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Elasto-inertial focusing and particle migration in high aspect ratio microchannels for highthroughput separation

Microsystems & Nanoengineering 2024年第3期10卷 381-394页

作者： Selim Tanriverdi Javier Cruz Shahriar Habibi Kasra Amini Martim Costa Fredrik Lundell Gustaf Mårtensson Luca Brandt Outi Tammisola Aman Russom division of nanobiotechnology Department of Protein ScienceScience for Life LaboratoryKTH Royal Institute of TechnologySolna 17165Sweden division of Microsystems Technology Department of Materials Science and EngineeringUppsala UniversityUppsala 75237Sweden FLOW and SeRC(Swedish e-Science Research Centre) Department of Engineering MechanicsRoyal Institute of TechnologyStockholm SE 10044Sweden FLOW and Fluid Physics Laboratory Department of Engineering MechanicsRoyal Institute of TechnologyStockholmSweden Wallenberg Wood Science Center Royal Institute of TechnologyStockholm SE 10044Sweden Department of Energy and Process Engineering Norwegian University of Science and Technology(NTNU)TrondheimNorway AIMES Center for the Advancement of Integrated Medical and Engineering Sciences at Karolinska Institutet and KTH Royal Institute of Technology StockholmSweden

The combination of flow elasticity and inertia has emerged as a viable tool for focusing and manipulating particles using *** there is considerable interest in the field of elasto-inertial microfluidics owing to its potential applications,research on particle focusing has been mostly limited to low Reynolds numbers(Re<1),and particle migration toward equilibrium positions has not been extensively *** this work,we thoroughly studied particle focusing on the dynamic range of flow rates and particle migration using straight microchannels with a single inlet high aspect *** initially explored several parameters that had an impact on particle focusing,such as the particle size,channel dimensions,concentration of viscoelastic fluid,and flow *** experimental work covered a wide range of dimensionless numbers(0.05

关键词： particle separation straight

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Development of a novel myconanomining approach for the recovery of agriculturally important elements from jarosite waste

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Journal of Environmental Sciences 2018年第5期30卷 356-367页

作者： Ankita Bedi Braj Raj Singh Sunil K.Deshmukh Nisha Aggarwal Colin J.Barrow Alok Adholeya TERI–Deakin nanobiotechnology Centre Biotechnology and Management of Bioresources Division The Energy and Resources Institute(TERI) School of Life and Environmental Sciences Deakin University Sri Aurobindo College Department of Chemistry University of Delhi

In this study, an ecofriendly and economically viable waste management approach have been attempted towards the biosynthesis of agriculturally important nanoparticles from jarosite waste. Aspergillus terreus strain J4 isolated from jarosite（waste from Debari Zinc Smelter,Udaipur, India）, showed good leaching efficiency along with nanoparticles（NPs） formation under ambient conditions. Fourier-transform infrared spectroscopy（FT-IR） and transmission electron microscopy（TEM） confirmed the formation of NPs. Energy dispersive X-ray spectroscopy（EDX analysis） showed strong signals for zinc, iron, calcium and magnesium,with these materials being leached out. TEM analysis and high resolution transmission electron microscopy（HRTEM） showed semi-quasi spherical particles having average size of 10‐50 nm. Thus, a novel biomethodology was developed using fungal cell-free extract for bioleaching and subsequently nanoconversion of the waste materials into nanostructured form. These biosynthesized nanoparticles were tested for their efficacy on seed emergence activity of wheat（Triticum aestivum） seeds and showed enhanced growth at concentration of 20 ppm. These nanomaterials are expected to enhance plant growth properties and being targeted as additives in soil fertility and crop productivity enhancement.

关键词： Bioleaching Fourier transform infraredspectroscopy (FTIR) Transmission electronmicroscopy (TEM) JarositeNanoparticles Seed-emergence activity

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New insight into surface properties of LB film of an amphiphilic terpolymer

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Chinese Chemical Letters 2011年第2期22卷 241-244页

作者： Xiu Li Yue Fang Ma, Zhi Fei Dai Meng Liu Lei Xing Hai Yan Fan Zhan Wen Xing Shao Qin Liu nanobiotechnology division State Key Laboratory of Urban Water Resources and Environment School of Sciences Harbin Institute of Technology Harbin 150001 China Received 4 June 2010

Amphiphilic terpolymer（TPR） exhibits good film-forming behavior on pure water observed by means ofπ-A *** gain insight into physical properties of TPR,the films have been deposited on silicon substrates at different surface pressure by Langmuir-Blodgett（LB） *** was found that the increase in peak intensities of stretching mode was due to orderly packing of the *** contact angles increased with increasing surface pressure,indicating an increase in hydrophobicity due to dense packing of chains of *** cyclic voltammetric measurements indicated that TPR showed good current shielding effect for *** a word,LB films of TPR can produce a variety of structures with varied topography,enabling us to control not only the functionality of the surface,but also the interfacial transport characteristics.

关键词： Terpolymer Monolayer Langmuir-Blodgett film Surface

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Targeted delivery of RNAi to cancer cells using RNA-ligand displaying exosome

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Acta Pharmaceutica Sinica B 2023年第4期13卷 1383-1399页

作者： Nasir Uddin Daniel WBinzel Dan Shu Tian-Min Fu Peixuan Guo Center for RNA nanobiotechnology and Nanomedicine Division of Pharmaceutics and PharmacologyCollege of Pharmacythe Ohio State UniversityColumbusOH 43210USA Dorothy M.Davis Heart and Lung Research Institute the Ohio State UniversityColumbusOH 43210USA James Comprehensive Cancer Center College of Medicinethe Ohio State UniversityColumbusOH 43210USA Department of Biological Chemistry&Pharmacology College of Medicinethe Ohio State UniversityColumbusOH 43210USA

Exosome is an excellent vesicle for in vivo delivery of therapeutics,including RNAi and chemical *** extremely high efficiency in cancer regression can partly be attributed to its fusion mechanism in delivering therapeutics to cytosol without endosome ***,being composed of a lipidbilayer membrane without specific recognition capacity for aimed-cells,the entry into nonspecific cells can lead to potential side-effects and *** engineering approaches for targeting-capacity to deliver therapeutics to specific cells is *** with chemical modification in vitro and genetic engineering in cells have been reported to decorate exosomes with targeting *** nanoparticles have been used to harbor tumor-specific ligands displayed on exosome *** negative charge reduces nonspecific binding to vital cells with negatively charged lipid-membrane due to the electrostatic repulsion,thus lowering the side-effect and *** this review,we focus on the uniqueness of RNA nanoparticles for exosome surface display of chemical ligands,small peptides or RNA aptamers,for specific cancer targeting to deliver anticancer therapeutics,highlighting recent advances in targeted delivery of siRNA and miRNA that overcomes the previous RNAi delivery *** understanding of exosome engineering with RNA nanotechnology promises efficient therapies for a wide range of cancer subtypes.

关键词： RNA interference RNA nanotechnology Endolysosome trapping Exosome engineering Targeted delivery Chemical drug delivery

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Stage-specific treatment of colorectal cancer: A microRNA-nanocomposite approach

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Journal of Pharmaceutical Analysis 2023年第11期13卷 1235-1251页

作者： Adewale Oluwaseun Fadaka Taiwo Akinsoji Ashwil Klein Abram Madimabe Madiehe Mervin Meyer Marshall Keyster Lucky Mashudu Sikhwivhilu Nicole Remaliah Samantha Sibuyi Department of Anesthesia Division of Pain ManagementCincinnati Children's Hospital Medical CenterCincinnatiOH45229USA Department of Science and Innovation/Mintek Nanotechnology Innovation Centre Biolabels NodeDepartment of BiotechnologyFaculty of Natural SciencesUniversity of the Western CapeBellville7535South Africa School of Medicine Southern Illinois UniversitySpringfieldIL62702USA Plant Omics Laboratory Department of BiotechnologyFaculty of Natural SciencesUniversity of the Western CapeBellville7535South Africa nanobiotechnology Research Group Department of BiotechnologyFaculty of Natural SciencesUniversity of the Western CapeBellville7535South Africa Environmental Biotechnology Laboratory Department of BiotechnologyFaculty of Natural SciencesUniversity of the Western CapeBellville7535South Africa Department of Science and Innovation/Mintek Nanotechnology Innovation Centre Advanced Materials DivisionMintekJohannesburg2125South Africa h Department of ChemistryFaculty of ScienceEngineering and AgricultureUniversity of VendaThohoyandou0950South Africa

Colorectal cancer(CRC)is among the leading causes of cancer *** lifetime risk of developing CRC is about 5%in adult males and *** is usually diagnosed at an advanced stage,and at this point therapy has a limited impact on cure rates and long-term *** and/or improved CRC therapeutic options are *** involvement of microRNAs(miRNAs)in cancer development has been reported,and their regulation in many oncogenic pathways suggests their potent tumor suppressor *** miRNAs provide a promising therapeutic approach for cancer,challenges such as biodegradation,specificity,stability and toxicity,impede their progression into clinical *** strategies offer diverse advantages for the use of miRNAs for CRC-targeted delivery and *** merits of using nanocarriers for targeted delivery of miRNA-formulations are presented herein to highlight the role they can play in miRNA-based CRC therapy by targeting different stages of the disease.

关键词： Colorectal cancer microRNA Nanotechnology Nanocarriers OncomiRs TSmiRs

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Genetic Analysis of 17 Children with Hunter Syndrome:Identification and Functional Characterization of Four Novel Mutations in the Iduronate-2-Sulfatase Gene

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Journal of Genetics and Genomics 2014年第4期41卷 197-203页

作者： Dimitry A.Chistiakov Lyudmila M.Kuzenkova Kirill V.Savost’anov Anait K.Gevorkyan Alexander A.Pushkov Alexey G.Nikitin Nato D.Vashakmadze Natalia V.Zhurkova Tatiana V.Podkletnova Leila S.Namazova-Baranova Alexander A.Baranove Department of Medical nanobiotechnology Pirogov Russian State Medical University Department of Molecular and Genetic Diagnostics Division of Laboratory MedicineInstitute of PediatricsResearch Center for Children’s Health Department of Psychoneurology and Psychosomatic Pathology Institute of PediatricsResearch Center for Children’s Health Institute of Preventive Pediatrics and Rehabilitation Research Center for Children’s Health Research Center for Children’s Health

Mucopolysaccharidosis type II （MPS II） is a rare X-linked disorder caused by alterations in the iduronate-2-sulfatase （IDS） gene. In this study, IDS activity in peripheral mononuclear blood monocytes （PMBCs） was measured with a fluorimetric enzyme assay. Urinary glycosaminoglycans （GAGs） were quantified using a colorimetric assay. All IDS exons and intronic flanks were bidirectionally sequenced. A total of 15 mutations （all exonic region） were found in 17 MPS II patients. In this cohort of MPS II patients, all alterations in the IDS gene were caused by point nucleotide substitutions or small deletions. Mutations ***88His and ***172＊ occurred twice. All mu- tations were inherited except for ***489Alafs＊7, a germline mutation. We found four new mutations （***142Phe, ***233Gly, ***430＊, and ***360Tyrfs＊31）. In Epstein-Barr virus （EBV）-immortalized PMBCs derived from the MPS II patients, no IDS protein was detected in case of the ***142Phe and ***360Tyrfs＊31 mutants. For ***233Gly and ***430＊, we observed a residual expression of IDS. The ***233Gly and ***430＊ mutants had a residuary enzymatic activity that was lowered by 14.3 and 76-fold, respectively, compared with healthy controls. This observation may help explain the mild disease phenotype in MPS II patients who had these two mutations whereas the ***142Phe and ***360Tyrfs＊31 mutations caused the severe disease manifestation.

关键词： Hunter syndrome Mucopolysaccharidosis type II Iduronate-2-sulfatase Mutations Glycosaminoglycans

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Photo-controlled release of paclitaxel and model drugs from RNA pyramids

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Nano Research 2019年第1期12卷 41-48页

作者： Congcong Xu Hui Li Kaiming Zhang Daniel W.Binzel Hongran Yin Wah Chiu Peixuan Guo Center for RNA nanobiotechnology and Nanotnedicine Division of Pharmaceutics and Pharmaceutical ChemistryCollege of PharmacyDorothy M.Davis Heart and Lung Research InstituteCollege of Medicine and James Comprehensive Cancer CenterThe Ohio State UniversityColumbusOhio 43210USA Departments of Bioengineering Microbiology and Immunologyand James H.Clark CenterStanford UniversityStanfordCA 94305USA SLAC National Accelerator Laboratory Stanford UniversityMenlo ParkCA 94025USA

Stimuli-resp on sive release of drugs from a nano carrier in spatial-,temporal-,and dosage-controlled fashi ons is of great interest in the pharmaceutical *** is one of the most effective and popular chemotherapeutic drugs against a number of cancers such as metastatic or non metastatic breast can cer,non-small cell lung can cer,refractory ovaria n cancer,AIDS-related Kaposi's sarcoma,and head and neck can ***,by taki ng the adva ntage of RNA nanotechno logy in biomedical and material scie nee,we developed a three-dime nsional pyramid-shaped RNA nanocage for a photocontrolled release of cargo,using paclitaxel as a model *** light-triggered release of paclitaxel or fluorophore Cy5 was achieved by incorporation of photocleavable spacers into the RNA *** irradiation with ultraviolet light,cargos were rapidly released(within 5 min).In vitro treatment of breast can cer cells with the RNA nano particles harbori ng photocleavable paclitaxel showed higher cytotoxicity as compared to RNA nanoparticles without the photocleavable *** methodology provides proof of con cept for the applicati on of the light-triggered con trolled release of drugs from RNA nano cages.

关键词： RNA nanotechnology controlled release paclitaxel drug delivery RNA nanoparticles Phi29 three-way junction

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Construction of RNA nanotubes

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Nano Research 2019年第8期12卷 1952-1958页

作者： Hui Li Shaoying Wang Zhouxiang Ji Congcong Xu Lyudmila S. Shlyakhtenko Peixuan Guo Center for RNA nanobiotechnology and Nanomedicine Division of Pharmaceutics and Pharmaceutical ChemistryCollege of Pharmacy/Department of Physiology & Cell BiologyCollege of Medicine/Dorothy M. Davis Heart and Lung Research Institute and James Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA UNMC Nanoimaging Core Facility Department of Pharmaceutical SciencesCollege of Pharmacy University of Nebraska Medical CenterOmahaNE68182USA Present address: University of California San FranciscoCA94158USA Present address: P&Z Biological Technology NewarkNJ07103USA

Nanotubes are miniature materials with significant potential applications in nanotechnological, medical, biological and material sciences. The quest for manufacturing methods of nano-mechanical modules is in progress. For example, the application of carbon nanotubes has been extensively investigated due to the precise width control, but the precise length control remains challenging. Here we report two approaches for the one-pot self-assembly of RNA nanotubes. For the first approach, six RNA strands were used to assemble the nanotube by forming a 11 nm long hollow channel with the inner diameter of 1.7 nm and the outside diameter of 6.3 nm. For the second approach, six RNA strands were designed to hybridize with their neighboring strands by complementary base pairing and formed a nanotube with a six-helix hollow channel similar to the nanotube assembled by the first approach. The fabricated RNA nanotubes were characterized by gel electrophoresis and atomic force microscopy (AFM), confirming the formation of nanotube-shaped RNA nanostructures. Cholesterol molecules were introduced into RNA nanotubes to facilitate their incorporation into lipid bilayer. Incubation of RNA nanotube complex with the free-standing lipid bilayer membrane under applied voltage led to discrete current signatures. Addition of peptides into the sensing chamber revealed discrete steps of current blockage. Polyarginine peptides with different lengths can be detected by current signatures, suggesting that the RNA-cholesterol complex holds the promise of achieving single molecule sensing of peptides.

关键词： RNA nanotechnology peptide sensing RNA nanotube nanobiotechnology

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3D RNA nanocage for encapsulation and shielding of hydrophobic biomolecules to improve the in vivo biodistribution

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Nano Research 2020年第12期13卷 3241-3247页

作者： Congcong Xu Kaiming Zhang Hongran Yin Zhefeng Li Alexey Krasnoslobodtsev Zhen Zheng Zhouxiang Ji Sijin Guo Shanshan Li Wah Chiu Peixuan Guo Center for RNA nanobiotechnology and Nanomedicine College of PharmacyDivision of Pharmaceutics and Pharmaceutical ChemistryCollege of MedicineDorothy M.Davis Heart and Lung Research InstituteJames Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA Department of Bioengineering James H.Clark CenterStanford UniversityStanfordCA94305USA Department of Physics University of Nebraska at OmahaOmahaNE68182USA Nanoimaging Core Facility Office of Vice-Chancellor for ResearchUniversity of Nebraska Medical CenterOmahaNE68198USA SLAC National Accelerator Laboratory Stanford UniversityMenlo ParkCA94025USA

Ribonucleic acid(RNA)nanotechnology platforms have the potential of harboring therapeutics for in vivo delivery in disease ***,the nonspecific interaction between the harbored hydrophobic drugs and cells or other components before reaching the diseased site has been an obstacle in drug *** we report an encapsulation strategy to prevent such nonspecific hydrophobic interactions in vitro and in vivo based on a self-assembled three-dimensional(3D)RNA *** placing an RNA three-way junction(3WJ)in the cavity of the nanocage,the conjugated hydrophobic molecules were specifically positioned within the nanocage,preventing their exposure to the biological *** assembly of the nanocages was characterized by native polyacrylamide gel electrophoresis(PAGE),atomic force microscopy(AFM),and cryogenic electron microscopy(cryo-EM)*** stealth effect of the nanocage for hydrophobic molecules in vitro was evaluated by gel electrophoresis,flow cytometry,and confocal *** in vivo sheathing effect of the nanocage for hydrophobic molecules was assessed by biodistribution profiling in *** RNA nanocages with hydrophobic biomolecules underwent faster clearance in liver and spleen in comparison to their ***,this encapsulation strategy holds promise for in vivo delivery of hydrophobic drugs for disease treatment.

关键词： ribonucleic acid(RNA)nanocage three-way junction(3WJ) encapsulation hydrophobic biomolecule RNA nanotechnology

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Non-invasive delivery of levodopa-loaded nanoparticles to the brain via lymphatic vasculature to enhance treatment of Parkinson’s disease

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Nano Research 2021年第8期14卷 2749-2761页

作者： Tianqi Nie Zhiyu He Jinchang Zhu Kuntao Chen Gregory P.Howard Jesus Pacheco-Torres II Minn Pengfei Zhao Zaver M.Bhujwalla Hai-Quan Mao Lixin Liu Yongming Chen School of Materials Science and Engineering Key Laboratory for Polymeric Composite and Functional Materials of Ministry of EducationSun Yat-sen UniversityGuangzhou 510275China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationOcean University of ChinaQingdao 266100China Department of Materials Science and Engineering Johns Hopkins University BaltimoreMD 21218USA Department of Biomedical Engineering Johns Hopkins School of Medicine BaltimoreMD 21205USA Institute for nanobiotechnology Johns Hopkins UniversityBaltimoreMD 21218USA division of Nuclear Medicine and Molecular Imaging Department of Radiology and Radiological Science Johns Hopkins School of MedicineBaltimoreMD 21205USA Translational Tissue Engineering Center Johns Hopkins School of MedicineBaltimoreMD 21287USA

Levodopa(L-DOPA),a precursor of dopamine,is commonly prescribed for the treatment of the Parkinson’s disease(PD).However,oral administration of levodopa results in a high level of homocysteine in the peripheral circulation,thereby elevating the risk of cardiovascular disease,and limiting its clinical ***,we report a non-invasive method to deliver levodopa to the brain by delivering L-DOPA-loaded sub-50 nm nanoparticles via brain-lymphatic *** hydrophilic L-DOPA was successfully encapsulated into nanoparticles of tannic acid(TA)/polyvinyl alcohol(PVA)via hydrogen bonding using the flash nanocomplexation(FNC)process,resulting in a high L-DOPA-loading capacity and uniform size in a scalable *** analysis in a PD rat model demonstrated that the levels of dopamine and tyrosine hydroxylase,which indicate the dopaminergic neuron functions,were increased by 2-and 4-fold,*** disorders and cerebral oxidative stress of the rats were significantly *** formulation exhibited a high degree of biocompatibility as evidenced by lack of induced inflammation or other pathological changes in major *** antioxidative and drug-delivery platform administered through the brain-lymphatic vasculature shows promise for clinical treatment of the PD.

关键词： levodopa brain delivery cerebral lymphatic vasculature Parkinson’s disease

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