检索结果-南通市图书馆

Effects of inter-culture, arabinogalactan proteins, and hydrogen peroxide on the plant regeneration of wheat immature embryos

引用

Journal of Integrative Agriculture 2015年第1期14卷 11-19页

作者： ZHANG Wei WANG Xin-min FAN Rong YIN Gui-xiang WANG Ke DU Li-pu XIAO Le-le YE Xing-guo National Key Facility of crop Gene Resources and Genetic improvement/Institute of crop Sciences Chinese Academy of Agricultural Sciences division of Agricultural and Environmental Science School of BiosciencesUniversity of Nottingham

The regeneration rate of wheat immature embryo varies among genotypes, howbeit many elite agriculture wheat varieties have low regeneration rates. Optimization of tissue culture conditions and attempts of adding signal molecules are effective ways to increase plant regeneration rate. Inter-culture is one of ways that have not been investigated in plant tissue culture. Moreover, the use of arabinogalactan proteins （AGPs） and hydrogen peroxide （H202） have been reported to increase regeneration rate in a few plant species other than wheat. The current research pioneeringly uses inter-culture of immature embryos of different wheat genotypes, and also investigates impacts of AGP and H2O2 on the induction of embryogenic calli and plant regeneration. As a result, high-frequency regeneration wheat cultivars Kenong 199 （KN 199） and Xinchun 9 （XC9）, together with low-frequency regeneration wheat line Chinese Spring （CS）, presented striking increase in the induction of embryogenic calli and plant regeneration rate of CS through inter-culture strategy, up to 52.19 and 67.98%, respectively. Adding 50 to 200 mg L-1 AGP or 0.005 to 0.01‰ H2O2 to the callus induction medium, enhanced growth of embryogenic calli and plant regeneration rate in quite a few wheat genotypes. At 50 mg L-1 AGP application level in callus induction medium plant regeneration rates of 8.49,409.06 and 283.16% were achieved for Jimai 22 （JM22）, Jingdong 18 （JD18） and Yangmai 18 （YM18）, respectively; whereas at 100 mg L-1 AGP level, CS （105.44%）, Chuannong 16 （CN16）（80.60%） and Ningchun 4 （NC4）（62.87%） acted the best. Moreover CS （79.05%）, JM22 （7.55%）, CN16 （101.87%）, YM18 （365.56%）, Yangmai 20 （YM20）（10.48%）, and CB301 （187.40%） were more responsive to 0.005 %o of H2O2, and NC4 （35.37%） obtained the highest shoot regeneration rates at 0.01%o of H2O2. Overall, these two methods, inter-culture and AGP （or H2O2） application, can be further applied to wheat transgenic research.

关键词： wheat immature embryos plant regeneration inter-culture arabinogalactan proteins hydrogen peroxide

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Life cycle greenhouse gas emissions from five contrasting rice production systems in the tropics

引用

Pedosphere 2023年第6期33卷 960-971页

作者： Pradeep K.DASH Pratap BHATTACHARYYA Soumya R.PADHY Amaresh K.NAYAK Annie POONAM Sangita MOHANTY crop Production division Indian Council of Agriculture Research(ICAR)-National Rice Research Institute(NRRI)GuttackOdisha 753006India

Carbon footprint (CF) quantification of major rice production systems (RPSs) is a prerequisite for developing strategies for climate change mitigation in agriculture. Total life cycle greenhouse gas emissions (LC-GHGs) from rice production to consumption might provide precise CFs for RPSs. Therefore, we assessed three segments (pre-farm, on-farm, and post-farm) of LC-GHGs under five major contrasting RPSs, i.e., aerobic rice (AR), shallow lowland rice(SLR), system of rice intensification (SRI), deep water rice (DWR), and zero-tilled direct-seeded rice (ZTR), in India to determine the corresponding CFs.Carbon footprint was the lowest for ZTR, while LC-GHGs were the lowest for AR. Therefore, AR is an adequate option for short-term reduction of GHG emissions. However, ZTR might be promoted by incentives as a long-term strategy. Among segmental LC-GHGs, on-farm GHG emissions contributed less than the other two segmental GHG emissions. The post-farm (i.e., farm gate to consumption) segment contributed the largest proportion (54%–69%) of total LC-GHGs, followed by pre-farm (i.e., cradle to farm) segment (21%–27%) and on-farm operation (11%–23%). These findings suggest that post-farm components that contribute to maximum GHG emissions must be scientifically tackled with proactive policy initiatives. However, the data of this segment are limited and scattered. Therefore, real-time assessment of GHG emissions during post-farm operation and input transportation from cradle to farm requires more precise quantification. Although CF in SRI was higher, this system had the potential to achieve higher yields and better soil carbon storage. Therefore,SRI may be encouraged from the perspectives of food security and long-term sustainability by reducing GHG emissions by three to four times.

关键词： carbon-equivalent emission carbon footprint climate change mitigation global warming management practice soil carbon stock:

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phytosterol content and the campesterol:sitosterol ratio influence cotton fiber development: role of phytosterols in cell elongation

引用

Science China(Life Sciences) 2016年第2期59卷 183-193页

作者： Shasha Deng Ting Wei Kunling Tan Mingyu Hu Fang Li Yunlan Zhai Shue Ye Yuehua Xiao Lei Hou Yan Pei Ming Luo Key Laboratory of Biotechnology and crop Quality improvement Ministry of AgricultureBiotechnology Research Center Southwest University

Phytosterols play an important role in plant growth and development,including cell division,cell elongation,embryogenesis,cellulose biosynthesis,and cell wall formation.Cotton fiber,which undergoes synchronous cell elongation and a large amount of cellulose synthesis,is an ideal model for the study of plant cell elongation and cell wall biogenesis.The role of phytosterols in fiber growth was investigated by treating the fibers with tridemorph,a sterol biosynthetic inhibitor.The inhibition of phytosterol biosynthesis resulted in an apparent suppression of fiber elongation in vitro or in planta.The determination of phytosterol quantity indicated that sitosterol and campesterol were the major phytosterols in cotton fibers;moreover,higher concentrations of these phytosterols were observed during the period of rapid elongation of fibers.Furthermore,the decrease and increase in campesterol:sitosterol ratio was associated with the increase and decease in speed of elongation,respectively,during the elongation stage.The increase in the ratio was associated with the transition from cell elongation to secondary cell wall synthesis.In addition,a number of phytosterol biosynthetic genes were down-regulated in the short fibers of ligon lintless-1mutant,compared to its near-isogenic wild-type TM-1.These results demonstrated that phytosterols play a crucial role in cotton fiber development,and particularly in fiber elongation.

关键词：植物甾醇细胞伸长菜油甾醇棉纤维醇含量生物合成抑制剂植物生长发育 β-谷甾醇

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rice ONAC016 promotes leaf senescence through abscisic acid signaling pathway involving OsNAP

引用

The crop Journal 2024年第3期12卷 709-720页

作者： Eunji Gi Sung-Hwan Cho Suk-Hwan Kim Kiyoon Kang Nam-Chon Paek Department of Agriculture Forestry and BioresourcesPlant Genomics and Breeding InstituteResearch Institute of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Republic of Korea division of Life Sciences Incheon National UniversityIncheon 22012Republic of Korea

Senescence-induced NAC(senNAC)TFs play a crucial role in senescence during the final stage of leaf development.In this study,we identified a rice senNAC,ONAC016,which functions as a positive regulator of leaf senescence.The expression of ONAC016 increased rapidly in rice leaves during the progression of dark-induced and natural senescence.The onac016-1 knockout mutant showed a delayed leaf yellowing phenotype,whereas the overexpression of ONAC016 accelerated leaf senescence.Notably,ONAC016 expression was upregulated by abscisic acid(ABA),and thus detached leaves of the onac016-1 mutant remained green much longer under ABA treatment.Quantitative RT-PCR analysis showed that ONAC016 upregulates the genes associated with chlorophyll degradation,senescence,and ABA signaling.Yeast one-hybrid and dual-luciferase assays revealed that ONAC016 binds directly to the promoter regions of OsNAP,a key gene involved in chlorophyll degradation and ABA-induced senescence.Taken together,these results suggest that ONAC016 plays an important role in promoting leaf senescence through the ABA signaling pathway involving OsNAP.

关键词： Rice ONAC016 OsNAP Leaf senescence Abscisic acid signaling

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Effects of Water-retaining Agents on Growth, Development, Yield and Quality of Soybean under Drought Stress

引用

Plant Diseases and Pests 2024年第3期15卷 34-39页

作者： Hui XIA Yan ZHANG Yun HAN Shasha HU Hengbin ZHANG Agricultural Science Research Institute of the Fourth division of the Xinjiang Production and Construction Corps Ili 835219China

[Objectives]The paper was to explore the effects of different doses of water-retaining agent on the growth and development indicators,yield and quality of soybean plants subjected to drought stress.[Methods]The effects of drought stress(MDS)and drought stress with low(MDS-L),medium(MDS-M)and high doses(MDS-H)of the water-retaining agent on the growth and development indicators,root activity,MDA content,antioxidant enzyme activity,yield and quality of soybean were studied by field plot test,with the normal water supply serving as the control(CK).[Results]In response to drought stress,the plant height,stem diameter,and yield of soybean exhibited a notable decline.Additionally,the contents of protein,fat,linoleic acid,and linolenic acid in seeds demonstrated a significant reduction.Conversely,the root activity and antioxidant enzyme activity exhibited a noticeable decline,while the MDA content exhibited an increase.The application of varying doses of the water-retaining agent was found to significantly enhance soybean growth,stimulate root activity,and elevate antioxidant enzyme activity,while concurrently reducing MDA content.The observed effects were found to be dose-dependent,with the greatest effects observed at the highest dose.In comparison to MDS,the yields of soybean in the MDS-L,MDS-M,and MDS-H treatments exhibited a 18.38%,25.58%,and 46.26%increase,respectively.Additionally,the content of protein,fat,linoleic acid,and linolenic acid in seeds demonstrated a notable enhancement.[Conclusions]The application of the water-retaining agent has been demonstrated to significantly promote the growth of soybean plants under conditions of drought stress,resulting in an improvement in both the yield and the quality of the soybean crop.The recommended dosage of the water-retaining agent is 3.3 kg/667 m 2.

关键词： Soybean Water-retaining agent Yield Quality Growth and development Antioxidant enzyme activity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

POLLEN STERILITY,a novel suppressor of cell division,is required for timely tapetal programmed cell death in rice

引用

Science China(Life Sciences) 2022年第6期65卷 1235-1247页

作者： Ronghui Che Bin Hu Wei Wang Yunhua Xiao Dapu Liu Wenchao Yin Hongning Tong Chengcai Chu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China National Key Facility for crop Gene Resources and Genetic improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China School of Biological and Science Technology University of JinanJinan 250022China College of Agriculture South China Agricultural UniversityGuangzhou510642China

Timely programmed cell death(PCD) of the tapetum supplying nutrients to microspores is a prerequisite for normal pollen development.Here we identified a unique mutant of rice(Oryza sativa L.),pollen sterility(post),which showed aborted pollens accompanied with extra-large husks.Due to failure of timely PCD of tapetal cells,post exhibited abnormal pollen wall patterning and defective pollen grains.By map-based cloning,we identified a causal gene,POST,encoding a novel protein which is ubiquitously localized in cells.RNA in situ hybridization showed that POST is highly detected in the tapetum and microspores at stages 8 and 9.Transcriptome analysis indicated that POST could function as an important regulator of the metabolic process involved in tapetal PCD.Compared with wild-type rice,post mutant has an increased cell number resulting from elevated expression of cell cycle associated genes in grain husks.Overexpression of POST inhibits grain size in wild type,while appropriate expression of POST in post mutant can recover the seed fertility but has little effect on the large grains,illustrating that fine-tuning of POST expression could be a potential strategy for rice yield improvement.The connection between cell division and cell death conferred by POST provides novel insights into the understanding of the tapetal PCD process.

关键词： programmed cell death tapetum pollen sterility grain size rice

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The TaTCP4/10-B1 cascade regulates awn elongation in wheat(Triticum aestivum L.)

引用

Plant Communications 2023年第4期4卷 255-270页

作者： Wensheng Ke Jiewen Xing Zhaoyan Chen Yidi Zhao Weiya Xu Lulu Tian Jinquan Guo Xiaoming Xie Dejie Du Zihao Wang Yufeng Li Jin Xu Mingming Xin Weilong Guo Zhaorong Hu Zhenqi Su Jie Liu Huiru Peng Yingyin Yao Qixin Sun Zhongfu Ni Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and Utilization(MOE)Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

Awns are important morphological markers for wheat and exert a strong physiological effect on wheat yield.The awn elongation suppressor B1 has recently been cloned through association and linkage analysis in wheat.However,the mechanism of awn inhibition centered around B1 remains to be clarified.Here,we identified an allelic variant in the coding region of B1 through analysis of re-sequencing data;this variant causes an amino acid substitution and premature termination,resulting in a long-awn phenotype.Transcriptome analysis indicated that B1 inhibited awn elongation by impeding cytokinin-and auxinpromoted cell division.Moreover,B1 directly repressed the expression of TaRAE2 and TaLks2,whose orthologs have been reported to promote awn development in rice or barley.More importantly,we found that TaTCP4 and TaTCP10 synergistically inhibited the expression of B1,and a G-to-A mutation in the B1 promoter attenuated its inhibition by TaTCP4/10.Taken together,our results reveal novel mechanisms of awn development and provide genetic resources for trait improvement in wheat.

关键词： TaTCP4/10 B1 awn length haplotype wheat

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dissection of heterosis for yield and related traits using populations derived from introgression lines in rice

引用

The crop Journal 2016年第6期4卷 468-478页

作者： Chao Xiang Hongjun Zhang Hui Wang Shaobo Wei Binying Fu Jiafa Xia Zefu Li Yongming Gao Guoyou Ye Institute of crop Sciences/National Key Facility for crop Gene Resources and Genetic improvement Chinese Academy of Agricultural Sciences Rice Research Institute Anhui Academy of Agricultural Sciences Genetics and Biotechnology division International Rice Research Institute

Despite the great success achieved by the exploitation of heterosis in rice,the genetic basis of heterosis is still not well understood.We adopted an advanced-backcross breeding strategy to dissect the genetic basis of heterosis for yield and eight related traits.Four testcross(TC) populations with 228 testcross F1 combinations were developed by crossing57 introgression lines with four types of widely used male sterile lines using a North Carolina II mating design.Analysis of variance indicated that the effects of testcross F1 combinations and their parents were significant or highly significant for most of the traits in both years,and all interaction effects with year were significant for most of the traits.Positive midparent heterosis(HMP) was observed for most traits in the four TC populations in the two years.The relative HMPlevels for most traits varied from highly negative to highly positive.Sixty-two dominant-effect QTL were identified for HMPof the nine traits in the four TC populations in the two years.Of these,22 QTL were also identified for the performance of testcross F1.Most dominant-effect QTL could individually explain more than 10% of the phenotypic variation.Four QTL clusters were observed including the region surrounding the RM9–RM297 region on chromosome 1,the RM110–RM279–RM8–RM5699–RM452 region on chromosome 2,the RM5463 locus on chromosome 6 and the RM1146–RM147 region on chromosome 10.The identified QTL for heterosis provide valuable information for dissecting the genetic basis of heterosis.

关键词： Rice Yield and related traits Introgression lines Heterosis Quantitative trait loci

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

NIGT1 represses plant growth and mitigates phosphate starvation signaling to balance the growth response tradeoff in rice

引用

Journal of Integrative Plant Biology 2023年第8期65卷 1874-1889页

作者： Yuxin Zhang Qianqian Zhang Meina Guo Xueqing Wang Tianjie Li Qingyu Wu Lihui Li Keke Yi Wenyuan Ruan National Key Facility for crop Gene Resources and Genetic improvement/Institute of crop Sciences Beijing 100081China State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China/Key Laboratory of Plant Nutrition and Fertilizer Ministry of AgricultureInstitute of Agricultural Resources and Regional PlanningBeijing 100081China National Engineering Research Center of Tree Breeding and Ecological Restoration College of Biological Sciences and TechnologyBeijing 100083China

Inorganic phosphate(Pi)availability is an important factor which affects the growth and yield of crops,thus an appropriate and effective response to Pi fluctuation is critical.However,how crops orchestrate Pi signaling and growth under Pi starvation conditions to optimize the growth defense tradeoff remains unclear.Here we show that a Pi starvationinduced transcription factor NIGT1(NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1)controls plant growth and prevents a hyper-response to Pi starvation by directly repressing the expression of growth-related and Pisignaling genes to achieve a balance between growth and response under a varying Pi environment.NIGT1 directly binds to the promoters of Pi starvation signaling marker genes,like IPS1,mi R827,and SPX2,under Pi-deficient conditions to mitigate the Pi-starvation responsive(PSR).It also directly represses the expression of vacuolar Pi efflux transporter genes VPE1/2 to regulate plant Pi homeostasis.We further demonstrate that NIGT1 constrains shoot growth by repressing the expression of growth-related regulatory genes,including brassinolide signal transduction master regulator BZR1,cell division regulator CYCB1;1,and DNA replication regulator PSF3.Our findings reveal the function of NIGT1 in orchestrating plant growth and Pi starvation signaling,and also provide evidence that NIGT1 acts as a safeguard to avoid hyper-response during Pi starvation stress in rice.

关键词： NIGT1 phosphate(Pi) PHRs Pi-starvation responsive(PSR) Pi starvation Pi signaling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anatomical studies of ovule development in the post-bloom pistils of the 'Zuili' plum (Prunus salicina Lindl.)

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第9期14卷 800-806页

作者： Hui-juan JIA Xia YANG Feng-jie HE Bin LI Key Laboratory of Horticultural Plant Growth Developmentand Quality Improvement of Ministry of AgricultureDepartment of HorticultureZhejiang University Taizhou Economic crop Station Jiaxing Economic crop Station

In order to investigate the cause of poor fruit set in 'Zuili' plums,anatomical examinations of post-bloom pistils were conducted and the dates of young fruit drop were recorded during the growing seasons of 2008 and 2009.Pistils of cv.'Black Amber' were also examined as an abundant setting control.Two major dropping periods were detected in 'Zuili':one during the first 5 d after full bloom (DAF) and another between 10 and 17 DAF.Anatomical analyses of the pistils at the full bloom stage revealed that half of the ovules had not developed embryos,which may have caused their early drop.In most dropped pistils collected at 17 DAF,the micropyle had not been penetrated by a pollen tube,indicating that they were not fertilized.'Zuili' ovules initiated embryo division at 10-12 DAF,although thereafter embryo development was retarded when compared to the rates observed in 'Black Amber'.Ovule fertilization failure and inactive embryo development after ovule fertilization may be the major causes of the later fruit drop observed in 'Zuili' plum trees.

关键词： Ovule Development Pistils ‘Zuili’ plum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：