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Nanoparticles-based delivery system for application of siRNA in vivo

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Nanoparticles-based delivery system for application of siRNA...

Single-atom Pd catalyst anchored on Zr-based metal-organic polyhedra for Suzuki-Miyaura cross coupling reactions in aqueous media

2010年广东省药师周大会

作者： Yee Han Leo Hwa Liang Aimin Ji division of bioengineering National University of Singapore Zhujiang Hospital Southern Medical University

Small interfering RNA (siRNA) silences the expression of specific target gene by mediating RNA interference (RNAi) in mammalian *** has not only been widely used as a valuable tool for functional genomics research,it has also demonstrated great potential in biomedical therapeutic application for diseases caused by gene abnormal overexpression or *** of the most important issues to fully fulfill its clinical application is how to effectively administer siRNAs to the target tissue or cells in vivo,which is highly dependent on the delivery ***,there are two major kinds of in vivo delivery system,which could be typed as viral or nonviral *** one of the nonviral-based carrier systems,nanoparticles developed through combination of liposome and cationic polymer complex,has exhibited improved in vivo stability,target specificity,and cell/tissue uptake and internalization of the encapsulated RNAi oligos,which result in more effective silencing efficacy with less cellular toxicity and immune *** review will discuss the latest advancement of nanoparticle-mediated RNAi delivery system,including nano-materials,preparation,*** conjunction,the clinical trial cases related to the nanoparticle-siRNA complexes will be *** safety issues of nanoparticles used in vivo will also be ***,this review will summarize the perspectives for its future application.

关键词： RNAi Small interfering RNA delivery system nanoparticles efficacy safety clinical trials.

来源： cnki会议评论

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Nano Research 2021年第2期14卷 486-492页

作者： Seongsoo Kim Seohyeon Jee Kyung Min Choi Dong-Sik Shin division of Chemical and bioengineering Kangwon National UniversityGangwon-do 24341Republic of Korea Department of Chemical and Biological Engineering Sookmyung Womens University100 Cheongpa-ro 47-gilYongsan-guSeoul 04310Republic of Korea

The challenge for single-atom catalysts in various C-C cross coupling reaction exists in the development of solid supporting *** has been desired tofind a supporting material designed in molecular level to anchor a single-atom catalyst and provide high degree of dispersion and substrate access in aqueous ***,we prepared discrete cages of metal-organic polyhedra anchoring single Pd atom(MOP-BPY(Pd))and successfully performed a Suzuki-Miyaura cross coupling reaction with various substrates in aqueous *** was revealed that each tetrahedral cage of MOP-BPY(Pd)has 4.5 Pd atoms on average and retained its high degree of dispersion up to 3 months in *** coupling efficiencies of the Suzuki-Miyaura cross coupling reaction exhibited more than 90.0%for various substrates we have tested in the aqueous media,which is superior to those of the molecular Pd complex and metal-organic framework(MOF)anchoring Pd ***,MOP-BPY(Pd)was successfully recovered and recycled without performance degradation.

关键词： metal-organic polyhedra Suzuki-Miyaura cross coupling reaction structure characterization aqueous phase reaction high-degree of dispersion

Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis

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World Journal of Stem Cells 2019年第10期11卷 803-816页

作者： Jeong Ah Kim Soohyun Hong Won Jong Rhee Research Center for Bioconvergence Analysis Korea Basic Science InstituteCheongju 28119South Korea Department of Bio-Analytical Science University of Science and TechnologyDaejeon 34113South Korea Program in Biomicro System Technology Korea UniversitySeoul 02841South Korea division of bioengineering Incheon National UniversityIncheon 22012South Korea Department of bioengineering and Nano-bioengineering Incheon National UniversityIncheon 22012South Korea

Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research,the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell microenvironment and the limited availability of high-throughput ***,researchers have been actively developing and evaluating three-dimensional(3D)cell culture-based platforms using microfluidic technologies,such as organ-on-a-chip and organoid-on-a-chip platforms,and they have achieved promising breakthroughs in stem cell *** this review,we start with a comprehensive discussion on the importance of microfluidic 3D cell culture techniques in stem cell research and their technical strategies in the field of drug *** a subsequent section,we discuss microfluidic 3D cell culture techniques for high-throughput analysis for use in stem cell *** addition,some potential and practical applications of organ-on-a-chip or organoid-on-a-chip platforms using stem cells as drug screening and disease models are highlighted.

关键词： Stem cell Microfluidic technology Three-dimensional cell culture Highthroughput screening

Carbene-catalyzed activation of cyclopropylcarbaldehydes for mannich reaction andδ-lactam formation:remote enantioselecitvity control and dynamic kinetic asymmetric transformation

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Science China Chemistry 2021年第6期64卷 985-990页

作者： Jie Lv Jun Xu Xuling Pan Zhichao Jin Yonggui Robin Chi State Key Laboratory Breeding Base of Green Pesticide and Agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinistry of EducationGuizhou UniversityGuiyang 550025China School of Pharmacy Guizhou University of Traditional Chinese MedicineGuiyang 550025China division of Chemistry&Biological Chemistry School of Physical&Mathematical SciencesNanyang Technological UniversitySingapore 637371Singapore

An N-heterocyclic carbene(NHC)-catalyzed enantioselective Mannich reaction of the remoteγ-carbon of cyclopropylcarbaldehydes is disclosed for the first ***-and enantiomerically enriched multicyclicδ-lactam compound is afforded as the main product from 8 possible stereo-specific isomers through dynamic kinetic asymmetric transformation(DYKAT)*** chiral functional molecules can be afforded from the lactam products through simple protocols with retentions of the optical purities.

关键词： N-heterocyclic carbene organocatalysis dynamic kinetic asymmetric transformation 1-cyclopropylcarbaldehydes asymmetric reactions

Destabilization of Basic Fibroblast Growth Factor by Oligodeoxynucleotides

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Chinese Chemical Letters 2006年第3期17卷 415-418页

作者： Qiang WU Ju WANG Ling ZHANG An HONG Jin Song REN Sub division of Biological Inorganic Chemistry Key Laboratory of Rare Earth Chemistry and Physics Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 bioengineering Institute of Jinan University Guangzhou 510632

The effects of oligodeoxynucleotide （ODN） on the conformation of basic fibroblast growth factor （bFGF） were studied by spectral method. The results showed that ODN destabilized the protein.

关键词： Basic fibroblast growth factor oligodeoxynucleotide (ODN) conformational changes

Regulation of inflammasomes by ubiquitination

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Cellular & Molecular Immunology 2016年第6期13卷 722-728页

作者： Joseph S Bednash Rama K Mallampalli division of Pulmonary Allergy and Critical Care Medicine Department of Medicine Acute Lung injury Center of Excellence University of Pittsburgh Pittsburgh PA 15213 USA Department of Cell Biology and Physiology and bioengineering University of Pittsburgh Pittsburgh PA 15213 USA medical Specialty Service Line Veterans Affairs Pittsburgh Healthcare System Pittsburgh PA 15240 USA

Inflammasomes are multi-protein complexes that regulate the innate immune response by facilitating the release of inflammatory cytokines in response to pathogen exposure or cellular damage. Pro-inflammatory inflammasome signaling is vital to host defense and helps initiate the process of tissue repair following an insult to the host, but can be injurious, when excessive or chronic. As such, inflammasome activity is tightly regulated. Here we discuss one critical mechanism of inflammasome regulation, ubiquitination, that functions as a universal modulator of protein stability and trafficking. Recent studies have provided important insights into the regulation of inflammasome activation by protein ubiquitination. We review the molecular regulation of inflammasome function, specifically, as it relates to ubiquitination, and discuss the implications for the development of theraoeutics to soecificallv target aberrant inflammasome signaling.

关键词： deubiquitinase E3 ligase inflammasome innate immunity ubiqutin

Bone density, microarchitecture and stiffness in Caucasian and Caribbean Hispanic postmenopausal American women

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Bone Research 2014年第3期2卷 194-202页

作者： Bin Zhou Ji Wang Emily M Stein Zhendong Zhang Kyle K Nishiyama Chiyuan A Zhang Thomas L Nickolas Elizabeth Shane X Edward Guo Bone bioengineering Laboratory Department of Biomedical Engineering Columbia University division of Endocrinology Department of Medicine Columbia University Department of Orthopedic Surgery First Affiliated Hospital School of Medicine Shihezi University

Hispanic Americans of Caribbean origin are a fast-growing subset of the US population, but there are no studies on bone density, microstructure and biomechanical integrity in this minority group. In this study, we aimed to compare Caucasian and Caribbean Hispanic postmenopausal American women with respect to these characteristics. Thirty-three Caribbean Hispanics were age-matched to thirty-three Caucasian postmenopausal women. At the lumbar spine, the Hispanic women had significantly lower areal bone mineral density （aBMD）. At the radius by high-resolution peripheral quantitative computed tomography （HR-pQCT）, there were minimal differences between Hispanic and Caucasian women. At the tibia, Hispanic women had lower trabecular volumetric bone density and trabecular number, and higher trabecular separation. Individual trabecula segmentation （ITS） analyses indicated that at the tibia, Hispanic women not only had significantly lower bone volume fraction, but also had significantly lower rod bone volume fraction, plate trabecular number, rod trabecular number and lower plate-plate, plate-rod and rod-rod junction densities compared to Caucasian women. The differences in bone quantity and quality contributed to lower whole bone stiffness at the radius, and both whole bone and trabecular bone stiffness at the tibia in Hispanic women. In conclusion, Hispanic women had poorer bone mechanical and microarchitectural properties than Caucasian women, especially at the load-bearing distal tibia.

关键词： bone DXA Bone density microarchitecture and stiffness in Caucasian and Caribbean Hispanic postmenopausal American women

N-Heterocyclic Carbene Organocatalysis: Activation Modes and Typical Reactive Intermediates

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Chinese Journal of Chemistry 2020年第10期38卷 1167-1202页

作者： Xingkuan Chen Hongling Wang Zhichao Jin Yonggui Robin Chi Department of Chemistry Jinan UniversityGuangzhouGuangdong 510632China Laboratory Breeding Base of Green Pesticide and Agricultural bioengineering Key Laboratory of Green Pesticide and Agricultural BioengineeringMinisry of EducationGuizhou UniversityHuaxi DistrictGuiyangGuizhou 550025China division of Chemistry&Biological Chemistr School of Physical&Mathematical SciencesNanyang Technological UniversitySingopore 637371Singapore

N-Heterocyclic carbene(NHC)organocatalysis has been developed as an important approach in modern organic *** activation modes within NHC organocatalysis have been established with countless transformations being realized in both efficient and selective *** would like to provide an overview on the key progresses achieved within this field in the past two *** numerous excellent reviews have been documented within this area,we will mainly focus on the scientific development of this research field based on the basic reaction modes and typical reaction intermediates.

关键词： synthesis. catalysis Heterocyclic

Daunorubicin can eliminate iPS-derived cancer stem cells via ICAD/CAD-independent DNA fragmentation

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Cancer Drug Resistance 2019年第2期2卷 335-350页

作者： Akimasa Seno Akifumi Mizutani Kazuki Aizawa Ryoma Onoue Junko Masuda Naotaka Ochi Saki Taniguchi Tatsuyuki Sota Yuki Hiramoto Taisuke Michiue Neha Nair Masaharu Seno Laboratory of Nano-Biotechnology Graduate School of Interdisciplinary Science and Engineering in Health SystemsOkayama UniversityOkayama 700-8530Japan division of medical bioengineering Graduate School of Natural Science and TechnologyOkayama UniversityOkayama 700-8530Japan.

Aim:To identify a drug that can effectively eliminate these cancer stem cells(CSCs)and determine its mode of ***:CSCs were obtained from mouse induced pluripotent stem cells(miPSCs)using cancer cell-conditioned *** screening was performed on these cells or after transplantation into *** was analyzed by flow cytometry and western ***:Drug screening studies showed that daunorubicin,a topoisomerase II inhibitor,is specifically cytotoxic to ***-induced apoptosis was found to be associated with p53 accumulation,activation of the caspase cascade,and oligonucleosomal DNA *** with the caspase inhibitor abolished daunorubicin-induced DNA fragmentation and was therefore considered to act downstream of caspase *** was also suppressed by treatment with a Ca2+-specific chelator,which suggested that CAD endonuclease does not ***,no obvious ICAD reduction/degradation was ***:Daunorubicin effectively eliminated CSCs,which are dependent on the p53/caspase signaling *** current findings provided the basis for further studies on CSC-targeted drugs for the development of cancer treatment strategies.

关键词： Daunorubicin cancer stem cell DNA fragmentation