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Light(Science & Applications) 2024年第6期13卷 1008-1011页

作者： Markus Sut. Inorganic Photoactive Materials Faculty of Mathematics and Natural SciencesHeinrich Heine University DusseldorfDusseldorfGermany

An ultra-simple and miniaturized spectrometer using an arbitrarily shaped pinhole as diffraction element reconstructs a broadband spectrum from the information of diffraction of monochromatic radiation by clever comput.tional reconstruction *** circumvents complex calibration procedures and paves the way to cost-effective on-chip spectrometers combining fast acquisition without.significant loss in spectral resolut.on.

关键词： arbitrarily shaped element

Genomic insights into the origin and evolut.on of spelt(Triticum spelta L.)as a valuable gene pool for modern wheat breeding

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Plant Communications 2024年第5期5卷 75-90页

作者： Yongfa Wang Zihao Wang Yongming Chen Tianyu Lan Xiaobo Wang Gang Liu Mingming Xin Zhaorong Hu Yingyin Yao Zhongfu Ni Qixin Sun Weilong Guo Huiru Peng Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and UtilizationBeijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China Institut. for Plant Genetics Heinrich Heine University40225 DusseldorfGermany Sanya Institut. of China Agricultural University Sanya 572025China

Spelt(Triticum aestivum ***)is an important wheat subspecies mainly cultivated in Europe before the 20th century that has contribut.d to modern wheat breeding as a valuable genetic ***,relatively little is known about.the origins and maintenance of spelt ***,using resequencing data from 416 worldwide wheat accessions,including representative spelt wheat,we demonstrate that Eu-ropean spelt emerged when primitive hexaploid wheat spread to the west and hybridized with pre-settled domesticated emmer,the put.tive maternal *** introgression regions from domesticated emmer confer spelt’s primitive morphological characters used for species taxonomy,such as tenacious glumes and laterﬂ*** propose a haplotype-based"spelt index"to identify spelt-type wheat vari-eties and to quantify ut.lization of the spelt gene pool in modern wheat *** study reveals the ge-netic basis for the establishment of the spelt wheat subspecies in a specific ecological niche and the vital role of the spelt gene pool as a unique germplasm resource in modern wheat breeding.

关键词： wheat spelt interspecific hybridization gene pool modern wheat breeding

GLUCOSE TRANSPORTER 2: PREVENTION OF STREPTOZOTOCIN-INDUCED REDUCTION OF 5-THIO-D-GLUCOSE

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Chinese Medical Journal 1995年第3期 82-82页

作者： Carsten Honack Peter Rosen Helga Gleichmann diabetes-forschungsinstit ut. heinrich-heine- universitat. dusseldorf GermanyDiabetes-Forschungsinstit ut. Heinrich-Heine- Universitat. Dusseldorf GermanyDiabetes-Forschungsinstit ut. Heinrich-Heine- Universitat. Dusseldorf Germany

Decreased expression of glucose transporter 2 (GLut.) has been found to correlate with impaired secreting β-cell function in diabetes. Here, the aim was to study if the GLut. of pancreatic islets is a target structure for streptozotocin (STZ) in vitro. GLLT2 expression was determined by Western blot analysis, using lysates prepared from islets of C57BL / 6mice. A dose-dependent decrement of GLut. expression was found after incubation of islets with streptozotocin for 30 min. Compared with solvent-incubated islets, a subtoxic dose (4mM) of STZ resulted in a reduction of approximate 58% of GLut. expression and a toxic dose

关键词： STZ GLUCOSE TRANSPORTER 2 PREVENTION OF STREPTOZOTOCIN-INDUCED REDUCTION OF 5-THIO-D-GLUCOSE

NUMERICAL APPROXIMATION OF THE SMOLUCHOWSKI EQUATION USING RADIAL BASIS FUNCTIONS

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Journal of Comput.tional Mathematics 2020年第1期38卷 176-194页

作者： Christiane Helzel Maximilian Schneiders Institut.of Mathematics Heinrich-Heine-Universitat DusseldorfGermany formerly Institut.of Mathematics Heinrich-Heine-Universitat DusseldorfGermany

The goal of this paper is to present a numerical method for the Smoluchowski equation,a drift-diffusion equation on the sphere,arising in the modelling of particle *** numerical method uses radial basis functions(RBF).This is a relatively new approach,which has recently mainly been used for geophysical *** a simplified model problem we compare the RBF approach with a spectral method,*** standard approach used in related physical *** comparison as well as our other accuracy studies show that RBF methods are an attractive alternative for these kind of models.

关键词： Smoluchowski equation Spectral method Radial basis function method

The transcription factor HSFA7b controls thermomemory at the shoot apical meristem by regulating ethylene biosynthesis and signaling in Arabidopsis

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Plant Communications 2024年第3期5卷 47-61页

作者： Sheeba John Federico Apelt Amit Kumar Ivan FAcosta Dominik Bents Maria Grazia Annunziata Franziska Fichtner Caroline Gut.ahr Bernd Mueller-Roeber Justyna J.Olas University of Potsdam Institute of Biochemistry and BiologyKarl-Liebknecht-Straße 24-25Haus 2014476 PotsdamGermany Max Planck Institut. of Molecular Plant Physiology Am Muehlenberg 114476 PotsdamGermany Laboratory of Molecular Biology Wageningen University6700 AP Wageningenthe Netherlands Center of Plant Systems Biology and Biotechnology(CPSBB) 14 St.Knyaz Boris 1 Pokrastitel Str.4023 PlovdivBulgaria Present address:Institut. of Plant Biochemistry Heinrich Heine University DusseldorfUniversit€atsstr.140225 DusseldorfGermany Leibniz Institut. of Vegetable and Ornamental Crops Theodor-Echtermeyer-Weg 114979 GroßbeerenGermany

The shoot apical meristem(SAM)is responsible for overall shoot growth by generating all aboveground *** research has revealed that the SAM displays an aut.nomous heat stress(HS)memory of a previous non-lethal HS *** the importance of the SAM for plant growth,it is essential to determine how its thermomemory is mechanistically ***,we report that HEAT SHOCK TRAN-SCRIPTION FACTOR A7b(HSFA7b)plays a crucial role in this process in Arabidopsis,as the absence of functional HSFA7b results in the temporal suppression of SAM activity after *** found that HSFA7b directly regulates ethylene response at the SAM by binding to the promoter of the key ethylene signaling gene ETHYLENE-INSENSITIVE 3 to establish ***,we demonstrated that HSFA7b regulates the expression of ETHYLENE OVERPRODUCER 1(ETO1)and ETO1-LIKE 1,both of which encode ethylene biosynthesis repressors,thereby ensuring ethylene homeostasis at the *** together,these results reveal a crucial and tissue-specic role for HSFA7b in thermomemory at the Arabidopsis SAM.

关键词： ethylene response heat stress adaptation shoot apical meristem SAM thermopriming thermomemory

New Insight in Ethylene Signaling： Aut.kinase Activity of ETR1 Modulates the Interaction of Receptors and EIN2

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Molecular Plant 2010年第5期3卷 882-889页

作者： Melanie M.A. Bisson Georg Groth heinrich-heine universitat.dusseldorf Biochemie der Pflanzen Universitatsstr. 1 40225 Dosseldorf Germany

Ethylene insensitive 2 （EIN2）, an integral membrane protein of the ER network, has been identified as the central regulator of the ethylene signaling pathway. Still, the mechanism by which the ethylene signal is transferred from the receptors to EIN2 has not been solved yet. Here, we show that protein phosphorylation is a key mechanism to control the interaction of EIN2 and the receptors. In vivo and in vitro fluorescence studies reveal that the kinase domain of the receptors is essential for the interaction. Cyanide, an ethylene agonist, which is known to reduce aut.-phosphorylation of the ethylene receptor ethylene resistant 1 （ETR1） or a mut.tion in the kinase domain of ETR1 that prevents aut.phosphorylation （H353A）, increases the affinity of the receptors for EIN2. On the other hand, mimicking permanent aut.-phosphorylation of ETR1 as in the mut.nt H353E releases the EiN2-ETR1 interaction from the control by the plant hormone. Based on our data, we propose a novel model on the integration of EIN2 in the ethylene signaling cascade.

关键词： Membrane biochemistry protein phosphorylation/dephosphorylation receptors signal transduction membrane proteins ethylene protein-protein interaction.

Optimizing laser coupling,matter heating,and particle acceleration from solids using multiplexed ultraintense lasers

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Matter and Radiation at Extremes 2024年第4期9卷 16-28页

作者： Weipeng Yao Motoaki Nakatsut.umi Sébastien Buffechoux Patrizio Antici Marco Borghesi Andrea Ciardi Sophia N.Chen Emmanuel d’Humières Laurent Gremillet Robert Heathcote Vojtech Horny Paul McKenna Mark N.Quinn Lorenzo Romagnani Ryan Royle Gianluca Sarri Yasuhiko Sentoku Hans-Peter Schlenvoigt Toma Toncian Olivier Tresca Laura Vassura Oswald Willi Julien Fuchs LULI-CNRS CEASorbonne UniversitéEcole PolytechniqueInstitut Polytechnique de ParisF-91128 Palaiseau CedexFrance Sorbonne Université Observatoire de ParisUniversité PSLCNRSLERMAF-75005 ParisFrance INRS-EMT 1650 BoulLionel-BouletVarennesQuebec J3X 1S2Canada Center for Plasma Physics School of Mathematics and PhysicsQueen’s University BelfastBelfast BT71NNUnited Kingdom “Horia Hulubei”National Institut. for Physics and Nuclear Engineering 30 Reactorului StreetRO-077125 Bucharest-MagureleRomania University of Bordeaux Centre Lasers Intenses et ApplicationsCNRSCEAUMR 5107F-33405 TalenceFrance CEA DAMDIFF-91297 ArpajonFrance Université Paris-Saclay CEALMCE91680 Bruyères-le-ChatelFrance Central Laser Facility STFC Rutherford Appleton LaboratoryDidcotUnited Kingdom SUPA Department of PhysicsUniversity of StrathclydeGlasgow G40NGUnited Kingdom Department of Physics University of NevadaRenoNevada 89557USA Institut. of Laser Engineering Osaka University2-6 YamadaokaSuitaOsaka 565-0871Japan Institut.fur Laser und Plasmaphysik Heinrich Heine Universitat DusseldorfDusseldorfGermany

Realizing the full potential of ultrahigh-intensity lasers for particle and radiation generation will require multi-beam arrangements due to technology ***,we investigate how to optimize their coupling with solid ***,we show that overlapping two intense lasers in a mirror-like configuration onto a solid with a large preplasma can greatly improve the generation of hot electrons at the target front and ion acceleration at the target *** underlying mechanisms are analyzed through multidimensional particle-in-cell simulations,revealing that the self-induced magnetic fields driven by the two laser beams at the target front are susceptible to reconnection,which is one possible mechanism to boost electron *** addition,the resistive magnetic field generated during the transport of the hot electrons in the target bulk tends to improve their *** simulations also indicate that such effects can be further enhanced by overlapping more than two laser beams.

关键词： laser acceleration particle

Machine Learning Techniques for Predicting Crop Photosynthetic Capacity from Leaf Reflectance Spectra

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Molecular Plant 2017年第6期10卷 878-890页

作者： David Heckmann Urte Schlut.r Andreas P.M. Weber heinrich-heine-universitat Institute for Computer Science heinrich-heine-universitat Institute of Plant Biochemistry Cluster of Excellence on Plant Sciences (CEPLAS) 'From Complex Traits towards Synthetic Modules' 40225 dusseldorf Germany

Harnessing natural variation in photosynthetic capacity is a promising rout. toward yield increases, but.physiological phenotyping is still too laborious for large-scale genetic screens. Here, we evaluate the potential of leaf reflectance spectroscopy to predict parameters of photosynthetic capacity in Brassica oleracea and Zea mays, a C3 and a C4 crop, respectively. To this end, we systematically evaluated properties of reflectance spectra and found that they are surprisingly similar over a wide range of species. We assessed the performance of a wide range of machine learning methods and selected recursive feature elimination on untransformed spectra followed by partial least squares regression as the preferred algorithm that yielded the highest predictive power. Learning curves of this algorithm suggest optimal species-specific sample sizes. Using the Brassica relative Moricandia, we evaluated the model transferability between spe- cies and found that cross-species performance cannot be predicted from phylogenetic proximity. The final intra-species models predict crop photosynthetic capacity with high accuracy. Based on the estimated model accuracy, we simulated the use of the models in selective breeding experiments, and showed that high-throughput.photosynthetic phenotyping using our method has the potential to greatly improve breeding success. Our results indicate that leaf reflectance phenotyping is an efficient method for improving crop photosynthetic capacity.

关键词： leaf spectrometry photosynthesis machine learning crops C4 phenotyping

Solid-state emissive biphenylene bridged bisaroyl-S,N-ketene acetals as distinct aggregation-induced enhanced emitters and fluorometric probes

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Aggregate 2021年第5期2卷 179-184页

作者： Lukas Biesen Thomas J.J.Muller Institut.fur Organische und Makromolekulare Chemie Heinrich-Heine-Universitat DusseldorfDüsseldorfGermany

Biphenylene bridged bisaroyl-S,N-ketene acetals can be readily synthesized by a one-pot Masuda borylation-Suzuki arylation sequence,thus,yielding a library of 20 bisaroyl-S,N-ketene acetals with tunable solid-state emission and aggregationinduced enhanced emission characteristics depending on the para substituents in the starting *** applications as fluorometric probe of alcoholic beverages are out.ined.

关键词： aggregation-induced emission aroyl-S N-ketene acetals liquor sensors one-pot reaction solid-state

Targeting Plant Ethylene Responses by Controlling Essential Protein-Protein Interactions in the Ethylene Pathway

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Molecular Plant 2015年第8期8卷 1165-1174页

作者： Melanie M.A. Bisson Georg Groth Institut. of Biochemical Plant Physiology Heinrich-Heine University Dusseldorf D-40204 Dusseldorf Germany

The gaseous plant hormone ethylene regulates many processes of high agronomic relevance throughout.the life span of plants. A central element in ethylene signaling is the endoplasmic reticulum （ER）-Iocalized membrane protein ETHYLENE INSENSITIVE2 （EIN2）. Recent studies indicate that in response to ethylene, the extra-membranous C-terminal end of EIN2 is proteolytically processed and translocated from the ER to the nucleus. Here, we report that the conserved nuclear localization signal （NLS） mediating nuclear import of the EIN2 C-terminus provides an important domain for complex formation with ethylene receptor ETHYLENE RESPONSE1 （ETR1）. EIN2 lacking the NLS domain shows strongly reduced affinity for the receptor. Interaction of EIN2 and ETR1 is also blocked by a synthetic peptide of the NLS motif. The corre- sponding peptide substantially reduces ethylene responses in planta. Our results uncover a novel mecha- nism and type of inhibitor interfering with ethylene signal transduction and ethylene responses in plants. Disruption of essential protein-protein interactions in the ethylene signaling pathway as shown in our study for the EIN2-ETR1 complex has the potential to guide the development of innovative ethylene antagonists for modern agriculture and horticulture.

关键词： fruit ripening ethylene responses peptide protein-protein interactions inhibitor