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Effects of feeding yeast(Saccharomyces cerevisiae), organic selenium and chromium mixed on growth performance and carcass traits of hair lambs

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Journal of Integrative Agriculture 2015年第3期14卷 575-582页

作者： Pedro A Hernández-García Alejandro Lara-Bueno Germán D Mendoza-Martínez José R Bárcena-Gama Fernando X Plata-Pérez Rufino López-Ordaz José A Martínez-García Faculty of Veterinary and Livestock (University Center) Autonomous University of the State of Mexico Department of animal Production Chapingo Autonomous University Department of Agriculture and Livestock Metropolitan Autonomous University (Xochimilco Unit) Institute of Genetic and Productivity (Livestock Center) Postgraduate College

Yeasts and organic minerals are used in diets to improve health, productive performance and some carcass characteristics of ruminants and non-ruminants. Thirty-two lambs（Pelibuey×Katahdin; BW=（30.55±1.67） kg; n=8） were used in a 56-d feeding experiment to study the effects of different levels of live yeast（Saccharomyces cerevisiae; yeast）, selenium（Se） and chromium（Cr） mixed（Se-Cr）, and a mixture of yeast-Se-Cr on growth performance and carcass traits. animals were stratified by body weight（BW） and randomly assigned to one of four treatments： 1） control group（0.0 g kg–1 yeast）; 2） yeast（1.50 g kg–1 dry matter intake（DMI） d–1）; 3） Se-Cr premix（1.5 mg kg–1 DMI d–1 for each mineral）; and 4） yeast-Se-Cr mixture. There were no treatment effects on final BW; whereas lambs fed Se-Cr or yeast-Se-Cr had higher（P〈0.05） DMI than animals supplemented with only yeast. Average daily gain（ADG）, gain：feed ratio, chop area, dorsal fat and carcass yield were similar（P〉0.05） among treatment groups. In conclusion, supplementation with yeast, Se-Cr mixed or yeast-Se-Cr did not improve ADG, final BW, back fat content and carcass yield of growing of Pelibuey×Katahdin lambs. Supplementation with Se-Cr and yeast-Se-Cr increased DMI, and approximately 250 g ADG animal–1 d–1 was produced with no negative effects on growth and health of the animals.

关键词： yeast organic selenium and chromium Pelibuey×Katahdin sheep

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Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock

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Chinese Journal of Oceanology and Limnology 2015年第4期33卷 919-927页

作者：李佳凯武祥伟谭茎赵瑞祥邓凌韦刘贤德 Key Laboratory of Mariculture in the East China Sea Ministry of Agriculture of China Fisheries College Jimei University animal Science and Technology College Yunnan Agricultural University Rice Molecular Breeding in Northern Chinese Academy of Sciences Joint Research Center

P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.

关键词：非纺织品热休克蛋白分子克隆反应表 cDNA克隆氨基酸残基氨基酸序列基因

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Effects of Compound Acidifier and Probiotics on Production Performance,Blood Biochemical Indexes,Antioxidant Indexes and Salmonella Infection of Laying Hens

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animal Husbandry and Feed Science 2017年第3期9卷 151-156页

作者： Zhou Ling Ding Xuemei Luo Yuheng Wang Jianping Bai Shiping Meng Huaiwang Zhang Keying animal Nutrition Institute Sichuan Agriculture University/Key Laboratory for Animal Disease-Resistance Nutritionthe Ministry of Education Chongqing Unibio Biotechnology Co. Ltd.

This study evaluated the effects of compound acidifier and probiotics on production performance, blood biochemical indexes, antioxidant indexes and Salmonella infection of laying hens. A total of 600 50-week-old healthy laying hens with similar laying rate were randomly assigned to four groups, 10 repeats each group, and each repeat contained 15 chickens. The laying hens in control group were fed a basal diet, and in the other three experimental groups were fed basal di- ets supplemented with 300 g/t probiotics, 0. 1% compound acidifier in the drinking water every other day, and 300 g/t probiotics ＋ 0.1% compound aeidifier in the drinking water every other day, respectively. After the feeding of 16 weeks, a total of 60 laying hens from control group were divided into four treatments as the same above for a 13-day long Salmonella challenge （1.3×10^8 CFU） trial. The results were shown as follows： no significant effects were observed on production performance, hemoglobin content, the superoxide dismutase activity and total antioxidant capacity of reproductive organ in compound acidifier and probiotics groups （ P 〉 0.05 ）. Dirty egg rate was significantly decreased in compound acidifier group, probiotics group and combination group of both （P 〈 0.05 ）. Compared with the control group, white blood cell count in probiotics group was significantly decreased （P 〈0.05 ）, and the immunoglobulin content in probiotics group tended to be increased （P = 0.061 ）. The malondialdehyde （MDA） content in oviduct isthmus of hens in compound acidifier group and combination group was significantly decreased compared with the control group （ P 〈 0.05 ）. The Salmonella positive rate in oviduct of hens in compound acidifier group was significantly decreased at the 7th and 14^th days （ P 〈0.05 ）, but that in probiotics group and combination group was not significantly different compared with control group （ P 〉 0.05 ）. In conclusion, compound acidifier and probiotics can improve health status

关键词： Laying hens Compound acidifier Probiotics Salmonella

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The omp2 Gene of HPS-type Bacteria Cloning and Sequence Analysis Isolates from Sichuan Province

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Engineering（科研） 2012年第10期4卷 153-158页

作者： Lirui Li Zexiao Yang Yin Wang Qiu Jin Xulong Wu Rongchang Zhang Sichuan Agricultural University Sichuan Laboratory of Animal Disease and Human Health SLADHH Ya'an China Tibet Changdu animal health and phytosanitary supervision TCAHPS Changdu China

In order to compare the homology and antigen of Haemophilus parasuis (HPS)4 outer membrane protein P2(omp2), we design a test with specific primers, using PCR amplification of isolates of Haemophilus parasuis from Sichuan Province(HP Sch2010), ompP2 gene will be cloned into the pGM-T vector, and transformed into E. coli DH5α. Identified by PCR and sequencing and analysis, the sequencing results showed that the published 4 HPS SW124 strains omp2 gene (1077bp), compared with the amplified 1086bp purpose fragment(containing omp2 genes), is relatively stable, with the nucleotide homology level 97% and amino acid homology level of 92.5%. The variable regions are mainly concentrated in the three base sequences: 40-65,110-156,180-202.

关键词： Haemophilus parasuis virulence genes omp2

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Acute exposure to high-peak-power pulsed microwaves affecting the histamine H_3 receptor expression in rat hippocampus

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Nuclear Science and Techniques 2006年第4期17卷 227-231页

作者： YU Xiao-Dong LI Bo LI De-Hua HE Qi-Yi YU Zheng-Ping Department of Occupational Health School of Preventive Medicine Third Military. Medical University Chongqing 400038China Chongqing Key Laboratory of animal Sciences School of Biological Science Chongqing Normal University Chongqing 400037 China

In the Morris Water Maze test, high-peak-power pulsed microwave (MW)-exposed rats displayed some learning and memory behavior dysfunctions, and their escape time and swimming distance to the submerged platform were longer than those of the sham-exposed rats. To understand the molecular mechanism involved, the reverse tran- scription-polymerase chain reaction (RT-PCR) and the Western-blotting technique were used for investigating the mRNA and protein expression patterns of the histamine H3 receptor (H3R) in rat hippocampus. High-peak-power pulsed microwave-exposure did not remarkably lead to the change in expression of H3R mRNA in rat hippocampi; however, it promoted the up-regulatory expression of the H3R protein, which was possibly triggered through the mi- togen-activated protein kinase (MAPK) pathways. Therefore, further investigation of the molecular mechanism of the MW effects on the learning and memory behaviors is required.

关键词：组胺H3受体大鼠海马微波辐照表达

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胆汁酸与肠道微生物的对话机制及其在宿主代谢与健康中的作用（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 436-446页

作者： Yang-fan NIE Jun HU Xiang-hua YAN College of animal Sciences and Technology Huazhong Agricultural University The Cooperative Innovation Center for Sustainable Pig Production

胆汁酸只在肝脏中合成,并通过直接作用或间接作用抑杀肠道微生物。而肠道微生物又可以促进初级胆汁酸转化为次级胆汁酸,从而改变机体内胆汁酸的组成。初级胆汁酸和次级胆汁酸均可调控法尼酯X核受体(FXR)的表达,已有研究发现,FXR在胆汁... 详细信息

胆汁酸只在肝脏中合成,并通过直接作用或间接作用抑杀肠道微生物。而肠道微生物又可以促进初级胆汁酸转化为次级胆汁酸,从而改变机体内胆汁酸的组成。初级胆汁酸和次级胆汁酸均可调控法尼酯X核受体(FXR)的表达,已有研究发现,FXR在胆汁酸的合成与代谢、葡萄糖代谢、脂质代谢和肝脏自噬中发挥着重要作用。此外,胆汁酸在肠道微生物的互作中起到了重要作用。本文主要综述了胆汁酸与肠道微生物之间的互作及其在宿主代谢调控中的作用,首次探讨了胆汁酸与肠道微生物的互作可能调节自噬相关信号通路。

关键词：胆汁酸法尼酯X核受体肠道微生物宿主代谢自噬

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Tu-Xian Decoction ameliorates diabetic cognitive impairment by inhibiting DAPK-1

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Chinese Journal of Natural Medicines 2023年第12期21卷 950-960页

作者： WANG Danyang YAN Bin WANG An SUN Qing PANG Junyi CUI Yangming TIAN Guoqing Department of Traditional Chinese Medicine Peking Union Medical College HospitalBeijing 100730China Chinese Academy of Mediucal Sciences&Peking Union Medical College Beijing 100730China Department of Pathology Peking Union Medical College HospitalState Key Laboratory of Complex Severe and Rare DiseaseMolecular Pathology Research CenterBeijing 100730China animal Research Laboratory Platform Peking Union Medical College Hospitalthe National Science and Technology Key Infrastructure on Translational MedicineBeijing 100730China

Tu-Xian decoction(TXD),a traditional Chinese medicine(TCM)formula,has been frequently administered to manage diabetic cognitive impairment(DCI).Despite its widespread use,the mechanisms underlying TXD’s protective effects on DCI have yet to be fully *** a significant regulator in neurodegenerative conditions,death-associated protein kinase-1(DAPK-1)serves as a focus for understanding the action of *** study was designed to whether TXD mediates its beneficial outcomes by inhibiting *** this end,a diabetic model was established using Sprague-Dawley(SD)rats through a high-fat,high-sugar(HFHS)diet regimen,followed by streptozotocin(STZ)*** experimental cohort was stratified into six groups:Control,Diabetic,TC-DAPK6,high-dose TXD,medium-dose TXD,and low-dose TXD *** a 12-week treatment period,various assessments—including blood glucose levels,body weight measurements,Morris water maze(MWM)testing for cognitive function,brain magnetic resonance imaging(MRI),and histological analyses using hematoxylin-eosin(H&E),and Nissl staining—were *** expression in the hippocampus was quantified through Western blotting *** results revealed that TXD significantly improved spatial learning and memory abilities,and preserved hippocampal structure in diabetic ***,TXD administration led to a down-regulation of proteins indicative of neurological damage and suppressed DAPK-1 activity within the hippocampal *** results underscore TXD's potential in mitigating DCI via DAPK-1 inhibition,positioning it as a viable therapeutic candidate for addressing this *** investigation into TXD's molecular mechanisms may elucidate new pathways for the treatment of DCI.

关键词： Tu-Xian decoction Traditional Chinese medicine Diabetes complications Diabetic cognitive impairment Death associated protein kinase-1

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Dynamic Gene Expression Profiles during Postnatal Development of Porcine Subcutaneous Adipose

Dynamic Gene Expression Profiles during Postnatal Developmen...

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第七届中国畜牧科技论坛

作者： Zhang jie Ma jideng Long keren Jin long Liu yihui Zhou chaowei Tian shilin Chen lei Luo zonggang Tang qianzi Jiang an'an Wang xun Wang dawei Jiang zhi Wang jinyong Li xuewei Li mingzhou Institute of animal Genetics and Breeding College of Animal Science and TechnologySichuan Agricultural University Department of animal Science Southwest University at Rongchang Department of Aquaculture Southwest University at Rongchang

A better understanding of the control of lipogenesis is of critical importance for both human and animal *** requires a better knowledge of the changes of gene expression during the process of adipose tissue ***,the objective of the current study was to determine the effects of development on subcutaneous adipose tissue gene ex-pression in growing and adult pigs. Here, we present a comprehensive investigation of mRNA transcriptomes in porcine subcutaneous adipose tissue across four developmental stages using digital gene expression profiling. We identified 3 274 differential expressed genes associated with oxidative stress, immune processes, apoptosis, energy metabolism, insulin stimulus,cell cycle, angiogenesis and translation(P<0. 05). A set of universally abundant genes(ACOX3,iVD』,A/D2,SCD and 7T/BAJB) was found across all four developmental stages. This set of genes may play important roles in lipogenesis and *** also identified development-related gene expression patterns that are linked to the different adipose phenotypes. We showed that genes enriched in significantly up-regulated profiles were associated with phosphorylation and angiogenesis. In contrast, genes enriched in significantly down-regulated profiles were related to cell cycle and cytoskeleton organization, suggesting an important role for these biological processes in adipose growth and development. These results provide a resource for studying adipose development and promote the pig as a model organism for researching the development of human obesity, as well as being used in the pig industry.

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Expression of Major Antigen Domains of E2 Gene of CSFV and Analysis of its Immunological Activity

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Virologica Sinica 2008年第4期23卷 247-254页

作者： Hong TIAN Xiang-tao LIU Jing-yan WU You-jun SHANG Tao JIANG Hai-xue ZHENG Qing-ge XIE Key Laboratory of animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences Lanzhou 730046 China

E2 is an envelope glycoprotein of Classical swine fever virus (CSFV) and contains sequential neutralizing epitopes to induce virus-neutralizing antibodies and mount protective immunity in the natural host. In this study, four antigen domains (ABCD) of the E2 gene was cloned from CSFV Shimen strain into the retroviral vector pBABE puro and expressed in eukaryotic cell (PK15) by an retroviral gene expression system, and the activity of recombinant E2 protein to induce immune responses was evaluated in rabbits. The results indicated that recombinant E2 protein can be recognized by fluorescence antibodies of CSFV and CSFV positive serum (Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China) using Western blot, indirect immunofluorescence antibody test (IFAT) and ELISA, Furthermore, anti-CSFV specific antibodies and lymphocyte proliferation were elicited and increased by recombinant protein after vaccination. In the challenge test, all of rabbits vaccinated with recombinant protein and Chinese vaccine strain (C-strain) were fully protected from a rabbit spleen virus challenge. These results indicated that a retroviral-based epitope-vaccine carrying the major antigen domains of E2 is able to induce high level of epitope-specific antibodies and exhibits similar protective capability with that induced by the C-strain, and encourages further work towards the development of a vaccine against CSFV infection.

关键词：猪瘟病毒 E2基因抗原逆转录酶病毒免疫活性

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Phytogenic Feed Additive for Sows： Effects on Sow Feed Intake, Serum Metabolite Concentrations, IgG Level, Lysozyme Activity and Milk Quality

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Journal of Agricultural Science and Technology(A) 2011年第6期1卷 802-810页

作者： M. Zhong D. Wu Y. Lin Z. F. Fang Institute of animal Nutrition Sichuan Agricultural University Ya ＇an 625014 China Key Laboratory for animal Disease Nutrition of Sichuan Province Ministry of Education Ya ＇an 625014 China

The objective of this study was to investigate the influence of a phytogenic feed additive (PFA) to late-gestation (d 90) and lactation sows on their reproduction performance. Sixty Large White × Landrace primiparous sows were divided into four groups (fifteen sows per group). The control group was fed with basal diet, the others were fed with basal diet supplemented with 0.02%, 0.04% and 0.06% PFA, respectively. Compared with the control, the sows in 0.04% PFA group had higher feed intake during lactation and higher litter weaning weight (P < 0.05). At farrowing, glucose level in 0.04% PFA sows was higher than the control and sows in 0.02% PFA had the highest IgG content among the treatments (P < 0.05). On day 7 of lactation, serum urea nitrogen contents were lower in response to PFA supplementation compared to the control (P < 0.05). At the same time, sows fed the 0.06% PFA diet increased the lysozyme activity (P < 0.05). The levels of milk lactose and IgG were increased in 0.02% and 0.04% PFA groups (P < 0.05). In conclusion, feeding PFA improved sows and litter performance, serum metabolite concentrations, IgG level and lysozyme activity at postpartum and milk quality.

关键词：母猪采食量血清代谢物溶菌酶活性 IgG含量饲料添加剂血糖水平牛奶质量浓度

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