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Effects of Heliotropium indicum *** Uterine Involution and Its Underlying Mechanisms:An in vivo and in vitro Study

Chinese Journal of Integrative Medicine 2023年第11期29卷 980-988页

作者： Sayah Ongsricharoenbhorn Pakanit Kupittayanant Kanjana Thumanu Griangsak Eumkeb Suthida Chanlun Pittaya Papirom Susan Wray Sajeera Kupittayanant School of Preclinical Sciences Institute of ScienceSuranaree University of TechnologyNakhon Ratchasima(30000)Thailand School of animal Technology and Innovation Institute of Agricultural TechnologySuranaree University of TechnologyNakhon Ratchasima(30000)Thailand Synchrotron Light Research Institute Nakhon Ratchasima(30000)Thailand Department of Pathobiology Faculty of Veterinary MedicineKhon Kaen UniversityKhon Kaen(40002)Thailand Department of Women's and Children's Health Harris-Wellbeing Preterm Birth CentreUniversity of LiverpoolLiverpoolL87SSUK

Objective:To investigate the effect of Heliotropium indicum L.(*** L.)on uterine involution and its underlying mechanisms in both in vivo and in vitro ***:For in vivo studies,postpartum rats were randomly divided into 2 groups(n=24 for each):control group and treated group which were orally and daily administered with ethanolic extract of *** L.(250 mg/kg body weight)until day 5 of *** were collected for analysis of weight,cross-sectional area,collagen cross-sectional area,and collagen content on postpartum day 1,3,and 5(n=8 for each)from both *** samples were collected for hepatotoxicity and 17?-estradiol(E_(2))*** in vitro studies,the extract effects on uterine contraction at half maximum effective concentration of 2.50 mg/mL were studied in organ bath system for at least 20 ***:Uterine parameters were significantly decreased after treated with extract of *** L.(P<0.05).*** *** significantly accelerated the reduction of those parameters and significantly decreased E_(2)(P<0.05).The extract facilitated uterine involution with no *** *** significantly stimulated uterine contraction(P<0.05)and synergized with oxytocin,prostaglandin and its precursor,linoleic *** investigating the different sequencing of the extract with the additional stimulants(added before or after),the two showed antagonistic effects,but still showed potentiated force when compared with control(without the stimulants).Conclusions:The underlying mechanisms by which *** *** uterine involution might be due to reducing E_(2)which induces collagenase activity,leading to decreases in uterine weight and size and stimulating uterine *** study provides new findings for future drug development for facilitating uterine involution with *** L.

关键词： measurement contraction sectional

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Variation in the goat KRTAP6-1 gene and its effect on wool traits in Longdong Cashmere goat

Variation in the goat KRTAP6-1 gene and its effect on wool t...

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2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Tao Li Shaobin Li Yuzhu Luo Jiqing Wang Xiu Liu Jiang Hu Faculty of animal Science and Technology Gansu Key Laboratory of Herbivorous Animal BiotechnologyGansu Agricultural University

Keratin-associated proteins（KAPs） are a key structural component of wool and hair fibres. They form a semi-rigid matrix in which the keratin intermediate filaments are embedded1 and hence they play an important role in determining fibre physico-mechanical properties. KAP6 belong to high glycine-tyrosine keratin-associated protein（HGT-KAP）. Some research found that the variations in KRTAP6 may have an effect on hair and wool traits in human, sheep and rabbit, but its effect on cashmere traits in goats is still unknown. PCR-SSCP and DNA sequencing technology were used to detect the variation of KRTAP6-1 in 304 Longdong Cashmere goat. GLMs were used to compare various cashmere traits among goats that had different KRTAP6-1 genotypes. Five PCR-SSCP banding patterns（A-E） and twelve single nucleotide polymorphisms（SNPs） of different sequences were identified in Longdong Cashmere goat. The presence of A was associated with an increase of cashmere fiber weight, and the genotype AB of goat had an effect on cashmere fiber weight and curly fiber length. This suggests that KRTAP6-1 may have important value for genetic markers of cashmere in goats.

关键词： KRTAP6-1 variation wool PCR-SSCP

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基于高质量系统发育基因组学数据鉴定的反刍动物特异性基因及其在瘤胃演化中的作用

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Science Bulletin 2022年第8期67卷 825-835,M0004页

作者：陈春燕殷源黎浩榕周博童周炯周小芳李志鹏刘贵春潘香羽张如林泽山陈垒邱强张勇王文 School of Ecology and Environment Northwestern Polytechnical UniversityXi’an 710072China College of animal Science and Technology Jilin Agricultural UniversityChangchun 130118China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China Department of Gastroenterology Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Guangdong Cardiovascular Institute Guangzhou 510080China Key Laboratory of Zoological Systematics and Evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Center for Excellence in animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China

反刍动物是世界上最成功的哺乳动物类群之一,具有显著的形态特征比如瘤胃.新基因在物种的表型演化中有重要的作用.为研究新基因在反刍动物新器官演化中的作用,结合新组装的鼷鹿和林麝的高质量基因组,以及以前发表的其他反刍动物科的高... 详细信息

反刍动物是世界上最成功的哺乳动物类群之一,具有显著的形态特征比如瘤胃.新基因在物种的表型演化中有重要的作用.为研究新基因在反刍动物新器官演化中的作用,结合新组装的鼷鹿和林麝的高质量基因组,以及以前发表的其他反刍动物科的高质量基因组,以牛科的绵羊基因注释为主,本工作鉴定了1064个反刍动物特异的新基因.结果显示,反刍动物新基因和其他哺乳动物中的新基因具有相似的演化和表达模式.有一部分新基因在瘤胃的演化中有作用.但大部分瘤胃表达的新基因招募自原先在其他组织表达的基因.如,在瘤胃不同发育时期有表达波动的反刍特异性PRD-SPRRⅡ基因家族成员受到正选择作用,可能在瘤胃的表皮发育中有重要功能.该工作的研究结果为理解反刍动物新基因的起源进化模式和哺乳动物新器官的演化提供了新的见解.

关键词： Ruminant High-quality ruminant genome New genes Rumen New organ evolution

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Avian Encephalomyelitis Virus Induces Apoptosis Via Major Structural Protein VP3

Avian Encephalomyelitis Virus Induces Apoptosis Via Major St...

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中国畜牧兽医学会禽病学分会第十一次学术研讨会

作者： Jimmy Kwang animal Health Biotechnology Laboratory

Avian encephalomyelitis virus (AEV) strain L2Z was investigated for its apoptotic activity in specific - pathogen - free (SPF) chick embryo brain tissue. DNA fragmentation analysis and electron microscopy observation demonstrated that AEV could induce apoptosis in chick embryo brain tissues characterized by chromatin condensation, plasma membrane blebbing, cell shrinkage, and nucleosomal DNA fragmentation 4 days post -infection (PI). AEV structural protein genes VP1, VP2 and VP3 were transfected into Cos-7 and chick embryo brain (CEB) cells, respectively. The results showed that only VP3 protein was an apoptotic inducer, as demonstrated by DNA fragmentation analysis and TUNEL assay at 24 and 48 hr post-transfection. Furthermore, expression of VP3 protein resulted in the activation of caspase-3 -like proteases in both cells, which could be inhibited by a caspase - 3 - like protease specific inhibitor Ac - DEVD-CHO peptide, suggesting that AEV VP3 protein induces apoptosis through a caspase -3 -like protease pathway. In addition, VP3 protein localized to mitochondria in the Cos -7 and CEB cells at 24 hr post-transfection observed by a confocal microscopy, indicating that mitochondria may play an important role in VP3 -induced apoptosis. Taken together, our results show that AEV could induce apoptosis in chick embryo brain tissue, structural protein VP3 could serve as an apoptotic inducer resulting in apoptosis in cell culture through a caspase -3 -like protease pathway, which may be related to its localization to mitochondria.

关键词： avian encephalomyelitis virus (AEV) and VPS protein chick embryo brain tissues apoptosis DEVDase activity mitochondria Cos-7 and chick embryo brain (CEB) cells.

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Characterization of the cDNA encoding a BPI/LBP homologue in venom gland of the hundred-pace snake Deinagkistrodon acutus

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Current Zoology 2009年第5期55卷 376-382页

作者： Jianrao HU Mingfu CAO Jiong CHEN Hangzhou Key Laboratory for animal Science and Technology College of Life and Environmental Sciences Hangzhou Normal University Hangzhou 310036 China Faculty of Life Science and Biotechnology Ningbo University Ningbo 315211 Zhejiang China

Bactericidal/permeability-increasing protein(BPI)and LPS-binding protein(LBP)play an important role in host *** evidence shows that BPI/LBP may be widely existed in different cells and tissue types of animals.A full-length cDNA clone encoding a BPI/LBP homologue(dBPI),1757 bp in size,was characterized in venom gland of the hundred-pace snake Deinagkistrodon *** deduced amino acid sequence of 417 residues had 13.8%-21.5% identity to BPI like 1(BPIL1)and BPI like 3(BPIL3)of other *** cysteine residues which are involved in disulfide bond formation between the final strand of the N-terminal beta sheet and the long alpha helix of BPI are identified as Cys146-Cys183 of *** tree analysis showed that the BPI/LBP homologues formed five large clusters and dBPI was in a large cluster including BPIL1 and *** mRNA shows a tissue specific expression in venom *** is the first study to identify the cDNA encoding BPI/LBP homologues from reptiles.

关键词：枸杞多糖基因编码 BPI 五步蛇同源毒液全长cDNA克隆杀菌/通透性增加蛋白

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Genetic variation at the p53 locus of two ecologically divergent Microtus pine voles:identification of molecular markers for species assignment

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Integrative Zoology 2022年第6期17卷 1179-1192页

作者： Ana Sofia QUINA João Paulo TAVANEZ Maria da Luz MATHIAS CESAM-Centro de Estudos do Ambiente e do Mar Departamento de BiologiaUniversidade de AveiroAveiroPortugal CESAM-Centro de Estudos do Ambiente e do Mar Faculdade de CiênciasUniversidade de LisboaLisboaPortugal Departamento de Biologia animal Faculdade de CiênciasUniversidade de LisboaLisboaPortugal Global Health and Tropical Medicine(GHTM) Instituto de Higiene e Medicina Tropical(IHMT)Universidade NOVA de Lisboa(UNL)Portugal

The Lusitanian(Microtus lusitanicus)and the Mediterranean(Microtus duodecimcostatus)pine voles are recently diverged sister species endemic of the Iberian Peninsula that can be identiﬁed with ecological and morphological characters,but in areas where the 2 species co-occur,species designation may be difﬁ*** discrimination between *** and *** has not been achieved yet possibly because of their estimated re-cent split and an evolutionary history that includes inter-species geneﬂ*** our previous observations on exons 5–7 of the p53 gene,here we analyze the potential use of the p53 genomic region as a discrimination marker of these species by extending our analyses to several kb upstream and downstream of the p53 gene and charac-terizing the degree of genetic differentiation in 7 markers within this ***,we fully sequenced the P53 protein of both *** observed:(i)generally high differentiation in this region;(ii)*** showed in general higher values of nucleotide and haplotype diversities;(iii)the concatenated phylogenetic tree separates the 2 species;(iv)the 2 P53 proteins only differ in 1 amino acid;(v)4 of the markers,2 in p53,one in Atp1b2,and another in Wrap53,contain species-speciﬁc genetic variation thus allowing a reliable discrimination between specimens from both species,irrespective of sampling location or introgression *** provide ad-ditional data on the putative role of p53 in the evolution of these species and present researchers with a fast and cost-effective resource for *** and *** identiﬁcation.

关键词： ecological stress genetic variation Microtus molecular marker p53

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Multiplex PCR Assay for the Simultaneous Detection of Five Pathogens in Laboratory animals

Multiplex PCR Assay for the Simultaneous Detection of Five P...

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第十三届中国实验动物科学年会

作者： Yan-Bo ZHU Long ZHENG Hong-Yu YOU Wei-Guang LIAN Jian-Min LIU Yan-Ning LI Yu-Rong MENG Dong-Ming ZHANG Jun-Xia WANG Hebei Key Laboratory for Laboratory animal Scinences Department of laboratory Animal Sciences Hebei Medical University

Staphylococcus aureus(S. aureus), Pseudomonas aeruginosa(P. aeruginosa), Bordetella bronchiseptica(B. bronchiseptica), Mycoplasma pneumoniae(M. pneumoniae), Pasteurella multocida(P. multocida) are commune pathogens isolated frequently in laboratory animals. Various diagnostic methods have been developed to identify these pathogens. In this report, we developed and evaluated a multiplex PCR for detecting these five pathogens in laboratory animals simultaneously, which is significant in differential diagnosis and screening. The primer concentrations and PCR conditions were optimized. The sensitivity of the multiplex PCR for simultaneous amplification is 10 pg of DNA per target. The specificity of the PCR was demonstrated by detecting pathogen-spiked clinical samples. A total of 182 clinical specimens were tested by the multiplex PCR, and the results were consistent with the results obtained using conventional bacterial isolation methods. In conclusion, the multiplex PCR developed in this report is a specific and sensitive method that can be used for differential diagnosis of five bacterial pathogen infections and screening in laboratory animals.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Bordetella bronchiseptica Mycoplasma pneumoniae Pasteurella multocida Multiplex PCR,differential diagnosis

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Effects of Dietary Energy Density in the Dry Period on the Production Performance and Metabolism of Dairy Cows

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Advances in Bioscience and Biotechnology 2017年第3期8卷 104-126页

作者： Yun Wang Qiuling Hou Gaozhan Cai Zhiyong Hu Kerong Shi Zhengui Yan Xueyan Lin Zhonghua Wang College of animal Science Shandong Agricultural University Taian China

Thirty healthy Holstein dairy cows in the dry period were randomly divided into three groups and fed diets with different net energy for lactation (group A: 1.2 Mcal/kg DM, group B: 1.3 Mcal/kg DM, and group C: 1.4 Mcal/kg DM) for 8 weeks prepartum. Thereafter, dairy cows were fed a diet of the same formulation (1.66 Mcal/kg DM) for 12 weeks postpartum. The effects of different dietary energy densities in the dry period on postpartum performance and metabolic parameters of dairy cows were observed. Milk yield was reduced by 14.5% in the low-energy diet group;however, there were no differences in milk composition between the three groups. Postpartum plasma β-hydroxybutyrate, non-esterified fatty acid, growth hormone, and glucagon levels were significantly decreased whereas leptin and neuropeptide levels were elevated in the low-energy diet group. Moreover, body fat mobilization was attenuated, and the decline in postpartum body condition was reduced in the low-energy diet group, thus effectively reducing the postpartum negative energy balance.

关键词： Dry Period Dairy Cows Energy Production Performance Biochemical Indicator Hormone Level

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Galectin-7 overexpression destroys airway epithelial barrier in transgenic mice

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Integrative Zoology 2021年第2期16卷 270-279页

作者： Jing TIAN Ruxuan HE Yimu FAN Qianqian ZHANG Baolin TIAN Chunju ZHOU Chunyan LIU Mingjing SONG Shunying ZHAO Department of Respiratory Medicine II Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina Virus Laboratory Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthChina Key Laboratory of Human Disease Comparative Medicine National Health Commission of China(NHC)Beijing Engineering Research Center for Experimental Animal Models of Human DiseasesInstitute of Laboratory Animal SciencePeking Union Medicine CollegeChinese Academy of Medical SciencesBeijingChina

When the integrity of airway epithelium is destroyed,the ordered airway barrier no longer exists and increases sensitivity to viral infections and allergens,leading to the occurrence of airway inflammation such as ***,we found that galectin-7 transgenic(+)mice exhibited abnormal airway structures as embryos and after *** abnormalities included absent or substantially reduced pseudostratified columnar ciliated epithelium and increased monolayer cells with irregular arrangement and widening of intercellular ***,airway tissue from galectin-7 transgenic(+)mice showed evidence of impaired cell–cell junctions and decreased expression of zonula occludens-1(ZO-1)and *** treated with respiratory syncytial virus(RSV)or ovalbumin(OVA),galectin-7 transgenic(+)mice developed substantially increased bronchial epithelial detachment and apoptosis,airway smooth muscle and basement membrane thickening,and enhanced airway *** found that Galectin-7 localized in the cytoplasm and nucleus of bronchial epithelial cells,and that increased apoptosis was mediated through mitochondrial release of cytochrome c and upregulated JNK1 activation and expression of caspase-3 in galectin-7 Tg(+)*** findings suggested that Galectin-7 causes airway structural defects and destroys airway epithelium barrier,which predispose the airways to RSV or OVA-induced epithelial apoptosis,injury,and other asthma responses.

关键词： airway epithelial barrier epithelial apoptosis galectin-7

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Genome-wide Analysis of Long Non-coding RNAs at Early Stage of Skin Pigmentation in Goats(Capra hircus)

Genome-wide Analysis of Long Non-coding RNAs at Early Stage ...

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第七届中国畜牧科技论坛

作者： Ren hangxing Wang gaofu Chen lei Jiang jing Liu liangjia Li nianfu Zhao jinhong Sun xiaoyan Zhou peng Chongqing Academy of animal Sciences Chongqing Engineering Research Center for Goats Youyang animal Husbandry Bureau

(Background) Long noncoding RNAs(IncRNAs) play roles in almost all biological processes;however,their function and profile in skin development and pigmentation is less *** addition,because IncRNAs are species-specific,their function in goats has not been established.(Result) We systematically identified IncRNAs in 100-day-old fetal skin by deep RNA-sequencing using the Youzhou dark goat(dark skin) and Yudong white goat(white skin) as a model of skin pigmentation.A total of 841,895,634 clean reads were obtained from six libraries(samples).We identified 1,336 specific IncRNAs in fetal skin that belonged to three subtypes,including 999 intergenic IncRNAs(lincRNAs),218 anti-sense IncRNAs,and 119 intronic *** results demonstrated significant differences in gene architecture and expression among the three IncRNA subtypes,particularly in terms of density and position bias of transpose elements near the transcription start *** also investigated the impact of IncRNAs on its target genes in cis and trans,indicating that these IncRNAs have a strict tissue specificity and functional conservation during skin development and pigmentation.(Conclusion) The present study provides a resource for IncRNA studies in diseases involved in pigmentation and skin *** expands our knowledge about IncRNA biology as well as contributes to the annotation of the goat genome.

关键词： skin IncRNA goat pigmentation transcriptome

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