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Journal of Ocean University of China 2024年第2期23卷 499-508页

作者： QIU Jiayin SUN Lianlian LI Shuang ZHOU Xu CHI Changfeng ZHENG Libing National and Provincial Joint Engineering Research center for Marine germplasm resources Exploration and Utilization School of Marine Science and TechnologyZhejiang Ocean UniversityZhoushan 316022China

Neuropeptides are widely distributed in vertebrates and invertebrates,regulating a variety of physiological activities in the organisms,such as metabolism,feeding and reproduction.In this study,to explore the function of neuropeptide LFRFamide in Sepia pharaonis,the full-length cDNA of LFRFamide-like gene(named SpLFRFL,MG869822.1)was identified with rapid amplification of cDNA ends(RACE)method.The sequence of SpLFRFL was 860 bp in length and encoded 188 amino acids containing 4 different mature peptides:1 copy of PHTPFRFamide,NSLFRFamide,TIFRFamide,and 3 copies of GNLFRFamide.Multiple alignment and phylogenetic analysis results showed that SpLFRFL shared high identity with LFRFamides of Sepia officinalis and Sepiella japonica and had the closest relationship with them.Through quantitative Real-time PCR(qRT-PCR),it was found that the SpLFRFL gene was highly expressed in the optic lobe and brain at three different stages during gonad development in both genders.Moreover,the four mature peptides at a concentration of 0.01μmol L^(−1) could inhibit the protein synthesis in the Chinese hamster ovary cell strain-K1(CHOK1)induced by SpGnRH.These data suggest that SpLFRFL might be involved in the development and reproduction of S.pharaonis.The results can contribute to future studies on neuropeptide evolution and function and benefit the cuttlefish farming.

关键词： neuropeptide LFRFamide-like cephalopod cuttlefish Sepia pharaonis development reproduction

Styrax japonicus functional genomics:an efficient virus induced gene silencing(VIGS)system

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Horticultural plant Journal 2024年第1期10卷 252-258页

作者： Gangyu Sun Yiqian Ju Cuiping Zhang Lulu Li Xinqiang Jiang Xiaoman Xie Yizeng Lu Kuiling Wang Wei Li College of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdaoShandong 266109China Shandong Provincial center of Forest and Grass germplasm resources JinanShandong 250102China

VIGS(Virus-induced gene silencing),a method for posttranscriptional gene silencing,is an effective technique for investigating the activities of genes in plants.Since there is no report for available VIGS system in Styrax japonicus,the application of a VIGS approach that results in a gene knockdown to study gene function is limited.In this study,we compared the characteristics that could affect the viability of VIGS in S.japonicus,including the acetosyringone(AS)concentration,the Agrobacterium’s optical density and the inoculation method.The stable reference genes of S.japonicus were selected to validate the gene’s knockdown by quantitative PCR.As a result,we successfully constructed 2 VIGS systems based on TRV virus:vacuum with AS concentration of 200μmol·L^(-1)and OD600of 0.5,and friction-osmosis with AS concentration of 200μmol·L^(-1)and OD600of 1.0,which silencing efficiency was 83.33%and 74.19%,respectively.The successfully applied VIGS method provides a rapid and effective reverse gene functional analysis approach in S.japonicus to identify unknown gene functions.

关键词： Styrax japonicus VIGS Reference gene

Pleistocene glaciation advances the cryptic speciation of Stellera chamaejasme L. in a major biodiversity hotspot

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Journal of Integrative plant Biology 2024年第6期66卷 1192-1205页

作者： Santosh Kumar Rana Hum Kala Rana Jacob B.Landis Tianhui Kuang Juntong Chen Hengchang Wang Tao Deng Charles C.Davis Hang Sun CAS Key Laboratory for plant Diversity and Biogeography of East Asia Kunming Institute of Botany Chinese Academy of Sciences Arkansas Biosciences Institute Arkansas State University School of Integrative plant Science Section of Plant Biology and the L.H.Bailey Hortorium Cornell University BTI Computational Biology center Boyce Thompson Institute CAS Key Laboratory of plant germplasm Enhancement and Specialty Agriculture Chinese Academy of Sciences Department of Organismic and Evolutionary Biology Herbaria Harvard University

The mountains of Southwest China comprise a significant large mountain range and biodiversity hotspot imperiled by global climate change. The high species diversity in this mountain system has long been attributed to a complex set of factors, and recent large-scale macroevolutionary investigations have placed a broad timeline on plant diversification that stretches from 10 million years ago(Mya) to the present. Despite our increasing understanding of the temporal mode of speciation, finer-scale populationlevel investigations are lacking to better refine these temporal trends and illuminate the abiotic and biotic influences of cryptic speciation. This is largely due to the dearth of organismal sampling among closely related species and populations, spanning the incredible size and topological heterogeneity of this region. Our study dives into these evolutionary dynamics of speciation using genomic and ecomorphological data of Stellera chamaejasme L. We identified four previously unrecognized cryptic species having indistinct morphological traits and large metapopulation of evolving lineages, suggesting a more recent diversification(～2.67–0.90 Mya), largely influenced by Pleistocene glaciation and biotic factors. These factors likely influenced allopatric speciation and advocated cyclical warming–cooling episodes along elevational gradients during the Pleistocene. The study refines the evolutionary timeline to be much younger than previously implicated and raises the concern that projected future warming may influence the alpine species diversity,necessitating increased conservation efforts.

关键词： cryptic speciation glacial-interglacial Hengduan Mountains north-south migration Stellera chamaejasme

Spatial-temporal shifting patterns and in situ conservation of spotted seal(Phoca largha)populations in the Yellow Sea ecoregion

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Integrative Zoology 2024年第2期19卷 307-318页

作者： Hongfei ZHUANG Fei SHAO Chao ZHANG Wancai XIA Shouqiang WANG Fangyuan QU Zongling WANG Zhichuang LU Linlin ZHAO Zhaohui ZHANG First Institute of Oceanography Ministry of Natural ResourcesQingdaoChina Department of Natural resources of Shandong Province Shandong Forestry Protection and Development Service CenterJinanChina National Park(Protected Areas)development center National Forestry and Grassland AdministrationBeijingChina College of Life Science China West Normal UniversityNanchongChina Liaoning Ocean and Fisheries Science Research Institute DalianChina

Understanding the habitat shifting pattern is a prerequisite for implementing in situ conservation of migratory species.Spotted seals(Phoca largha)inhabiting the Yellow Sea ecoregion(YSE)comprise a small population with independent genes and represent a charismatic flagship species in this region.However,this population has declined by 80%since the 1940s,and increased support from the countries around the YSE is urgently needed to address the potential local extinction risk.A time-series niche model and life-history weighted systematic conservation planning were designed on the basis of a satellite beacon tracking survey(2010–2020)of the YSE population.The results showed clustering and spreading shifting patterns during the breeding and migratory seasons,respectively.The closed-loop migration route formed in the YSE indicated that this population might be geographically isolated from populations in other breeding areas around the world.The conservation priority area(CPA),with an area of 19632 km^(2)(3.58%of the total YSE area),was the most effective response to the potential in situ risk.However,nearly 80%of the CPA was exposed outside the existing marine protected areas(MPAs).Future establishment of MPAs in China should strategically consider the conservation gap identified herein,and it is recommended for Korea’s closed fishing season to be spatially set in the western Korean Peninsula from May to August.This study also exemplified that the lack of temporal information would lead to the dislocation of niche modeling for migratory species represented by spotted seals.Attention should be paid to protecting small and migratory populations in marine biodiversity conservation planning.

关键词： conservation priority area marine protected area migration seasonal management spotted seal

Arbuscular mycorrhizal fungi inoculation and exogenous indole-3-acetic acid application induce antioxidant defense response to alleviate cadmium toxicity in Broussonetia papyrifera

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Pedosphere 2024年第2期34卷 447-459页

作者： Xue LI Jingwei LIANG Hongjian WEI Yuxuan KUANG Hui CHEN Ming TANG Wentao HU State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern AgricultureGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant GermplasmCollege of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642China

Cadmium(Cd)contamination in soil poses a huge threat to plants even at low concentrations;Broussonetia papyrifera has great potential in remediation of soil heavy metal contamination.However,whether exogenous indole-3-acetic acid(IAA)application and arbuscular mycorrhizal fungi(AMF)have synergistic effects on Cd tolerance of B.papyrifera remains unclear.To investigate the effects of AMF inoculation and IAA application on the tolerance of B.papyrifera to Cd stress,two experiments were conducted:the first to investigate the effect of AMF(Rhizophagus irregularis)inoculation on the tolerance of B.papyrifera to Cd stress and the second to investigate the combined effects of AMF inoculation and IAA application on the tolerance of B.papyrifera to Cd stress.Parameters including endogenous hormone concentration,antioxidant defense response,malondialdehyde(MDA)content,and gene expression related to antioxidant enzyme system and hormone were measured.The results indicated that AMF alleviated Cd toxicity of B.papyrifera by reducing MDA content and improving antioxidant enzyme activities and Cd absorption capacity.Furthermore,the combination of AMF inoculation and IAA application had a synergetic effect on the tolerance of B.papyrifera to Cd stress through upregulating BpAUX1 and BpAUX2,which might contribute to root growth and root xylem synthesis,and by upregulating BpSOD2 and BpPOD34 to enhance the antioxidant enzyme system.This work provides a new insight into the application of IAA in the remediation of soil Cd pollution by mycorrhizal plants.

关键词： enzyme activity geneexpression heavy metal phytoremediation remediation Rhizophagus irregularis soil cadmium pollution stress tolerance

Impact of mechanical compaction on crop growth and sustainable agriculture

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Frontiers of Agricultural Science and Engineering 2024年第2期11卷 243-252页

作者： Zijian LONG Yifei WANG Baoru SUN Xiaoyan TANG Kemo JIN College of resources and Environmental Sciences National Academy of Agriculture Green Development Key Laboratory of Plant–Soil Interactions (Ministry of Education) China Agricultural University College of resources Sichuan Agricultural University

With the development of agricultural technology to meet the growing demands of a rapidly increasing population and economic development,intensive agriculture practices have been widely adopted globally. However,this intensification has resulted in adverse consequences for soil structure due to intensified farming activities and increased usage of heavy farm machinery.Of particular concern is soil compaction, which leads to the degradation of physical, chemical and biological properties of the soil. Soil compaction negatively impacts crop growth, reduces yields and poses a significant threat to food security and the overall sustainability of agricultural systems.Recognizing these challenges, this review aims to deepen understanding of the factors contributing to soil compaction and to develop effective mitigation strategies. By doing so, it is intended to attenuate the adverse impacts of soil compaction, improve soil structure, increase crop yield and ultimately enhance the sustainability of agricultural practices.

关键词： Soil compaction mechanical compaction bio-tillage agricultural production

The Ti-TAN plasmid toolbox for TurboID-based proximity labeling assays in Nicotiana benthamiana

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Journal of Integrative plant Biology 2024年第2期66卷 166-168页

作者： Huang Tan Yu Zhou Erik Dinius Rosa Lozano-Durán Department of plant Biochemistry Center for Plant MolecularBiology(ZMBP)Eberhard Karls UniversityTubingenD‐72076Germany

Protein-protein interactions and the formation of protein complexes play a central role in the regulation of virtually all aspects of life.The identification and characterization of interactions between proteins is th... 详细信息

关键词： proximity complexes enable

Woody plant cell walls:Fundamentals and utilization

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Molecular plant 2024年第1期17卷 112-140页

作者： Wei Li Ying-Chung Jimmy Lin Ying-Lan Chen Chenguang Zhou Shuang Li Nette De Ridder Dyoni M.Oliveira Lanjun Zhange Baocai Zhang Jack P.Wang Changzheng Xu Xiaokang Fu Keming Luo Ai-Min Wu Taku Demura Meng-Zhu Lu Yihua Zhou Laigeng Li Toshiaki Umezawa Wout Boerjan Vincent L.Chiang State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040China Department of Life Science National Taiwan UniversityTaipeiTaiwanChina Department of Biotechnology and Bioindustry Sciences College of Bioscience and BiotechnologyNational Cheng Kung UniversityTainanTaiwanChina Department of plant Biotechnology and Bioinformatics Ghent UniversityTechnologiepark 719052 GhentBelgium VIB center for plant Systems Biology Technologiepark 719052 GhentBelgium State Key Laboratory of plant Genomics Insttute of Genetics and developmental Biology Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Forest Biotechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA Chongqing Key Laboratory of plant Resource Conservation and germplasm Innovation Integrative Science Center of Germplasm CreationSchool of Life SciencesSouthwest UniversityChongqing 400715China Guangdong Key Laboratory for Innovative developmentand Utilization of Forest plant germplasm College of Forestry and Landscape ArchitecturesSouth China Agricultural UniversityGuangzhou510642China center for Digital Green-innovation Nara Institute of Science and TechnologykomaNara 630-0192Japan State Key Laboratory of Subtropical Siviculture College of Forestry and BiotechnologyZhejang A&F UniversityHangzhou 311300China CAS center for Excellence in Molecular plant Sciences Chinese Academy of Sciences300 Fenglin RoadShanghai 200032China Laboratoryof MetabolicScienceofForest plants and Microorganisms Rsearch Institute for Sustainable HumanosphereKyoto UniversityUjiKyoto 611-0011 Japan

Cell walls in plants,particularly forest trees,are the major carbon sink of the terrestrial ecosystem.Chemical and biosynthetic features of plant cell walls were revealed early on,focusing mostly on herbaceous model species.Recent developments in genomics,transcriptomics,epigenomics,transgenesis,and associated analytical techniques are enabling novel insights into formation of woody cell walls.Here,we review multilevel regulation of cell wall biosynthesis in forest tree species.We highlight current approaches to engineering cell walls as potential feedstock for materials and energy and survey reported field tests of such engineered transgenic trees.We outline opportunities and challenges in future research to better understand cell type biogenesis for more efficient wood cell wall modification and utilization for biomaterials or for enhanced carbon capture and storage.

关键词： regulation of cell wall formation biosynthesis of cell wall components lignin cellulose hemicellulose cell wall utilization

How plants sense and respond to osmotic stress

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Journal of Integrative plant Biology 2024年第3期66卷 394-423页

作者： Bo Yu Dai-Yin Chao Yang Zhao Shanghai center for plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai 200032China Key Laboratory of plant Carbon Capture The Chinese Academy of SciencesShanghai 200032China National Key Laboratory of plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyThe Chinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China

Drought is one of the most serious abiotic stresses to land plants.plants sense and respond to drought stress to survive under water deficiency.Scientists have studied how plants sense drought stress,or osmotic stress caused by drought,ever since Charles Darwin,and gradually obtained clues about osmotic stress sensing and signaling in plants.Osmotic stress is a physical stimulus that triggers many physiological changes at the cellular level,including changes in turgor,cell wall stiffness and integrity,membrane tension,and cell fluid volume,and plants may sense some of these stimuli and trigger downstream responses.In this review,we emphasized water potential and movements in organisms,compared putative signal inputs in cell wall-containing and cell wall-free organisms,prospected how plants sense changes in turgor,membrane tension,and cell fluid volume under osmotic stress according to advances in plants,animals,yeasts,and bacteria,summarized multilevel biochemical and physiological signal outputs,such as plasma membrane nanodomain formation,membrane water permeability,root hydrotropism,root halotropism,Casparian strip and suberin lamellae,and finally proposed a hypothesis that osmotic stress responses are likely to be a cocktail of signaling mediated by multiple osmosensors.We also discussed the core scientific questions,provided perspective about the future directions in this field,and highlighted the importance of robust and smart root systems and efficient source-sink allocations for generating future high-yield stress-resistant crops and plants.

关键词： cell volume drought membrane tension osmotic stress turgor

High-quality assembled and annotated genomes of Nicotiana tabacum and Nicotiana benthamiana reveal chromosome evolution and changes in defense arsenals

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Molecular plant 2024年第3期17卷 423-437页

作者： Jubin Wang Qingling Zhang Jeffrey Tung Xi Zhang Dan Liu Yingtian Deng Zhendong Tian Huilan Chen Taotao Wang Weixiao Yin Bo Lij Zhibing Lai Savithramma P.Dinesh-Kumar Barbara Baker Feng Li National Key Laboratory for germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China plant Gene Expression center Department of Plant and Microbial BiologyUniversity of CaliforniaBerkeleyBerkeleyCA 94706USA College of plant Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China College of Life Science and Technology Huazhong Agricultural UniversityWuhanHubei 430070China Department of plant Biology and The Genome center College of Biological SciencesUniversity of CalforniaDavisDavisCA 95616USA The Key Laboratory of Horticultural plant Genetic and Improvement of Jiangxi Province Institute of Biological ResourcesJiangxi Academy of SciencesNanchang 330299China institute of Vegetables and Flowers Jiangxi Academy of Agricultural SciencesNanchang 330200Chin Hubei Hongshan Laboratory WuhanHubei 430070China

Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology research.However,genomic studies of these species have lagged.Here we report the chromosome-level reference genome assemblies for N.benthamiana and N.tabacum with an estimated 99.5%and 99.8%completeness,respec-tively.Sensitive transcription start and termination site sequencing methods were developed and used for accurate gene annotation in N.tabacum.Comparative analyses revealed evidence for the parental origins and chromosome structural changes,leading to hybrid genome formation of each species.Interestingly,theantiviral silencinggenesRDR1,RDR6,DCL2,DCL3,andAGO2were lost from one or both subgenomes in N.benthamiana,while both homeologs were kept in N.tabacum.Furthermore,the N.benthamiana genome encodes fewer immune receptors and signaling components than that of N.tabacum.These find-ings uncover possible reasons underlying the hypersusceptible nature of N.benthamiana.We developed the user-friendly Nicomics(http:/***/Nicomics/)web server to facilitate better use of Nicotiana genomic resources as well as gene structure and expression analyses.

关键词： Nicotiana tabacum Nicotiana benthamiana genomics evolution disease resistance NLR