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Precise determination of the pole position of the exotic Z_(c)(3900)

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Science China(Physics,Mechanics & Astronomy) 2024年第9期67卷 84-90页

作者： Yun-Hua Chen Meng-Lin Du Feng-Kun Guo School of Mathematics and Physics University of Science and Technology BeijingBeijing 100083China School of Physics University of Electronic Science and Technology of ChinaChengdu 611731China CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Peng Huanwu Collaborative center for research and Education Beihang UniversityBeijing 100191China

We perform a unified description of the experimental data of the π^(+)π^(-)and J/ψπ^(±)invariant mass spectra for e^(+)e^(-)→J/ψπ^(+)π^(-)and the D^(0)D^(*-)mass spectrum for e^(+)e^(-)→D^(0)D^(*-)π^(+)at e^(+)e^(-)center-of-mass energies 4.23 and 4.26 *** analysis takes into account open-charm meson loops that contain triangle singularities,the J/ψπ-DD^(*)coupled-channel interaction respecting unitarity,and the strong ππ-KK final state interaction using dispersion *** analysis leads to a precise determination of the Z_(c)(3900)pole with the pole mass and width(3880.7±1.7_(stat)±22.4_(syst))MeV and(35.9±1.4_(stat)±15.3_(syst))MeV,respectively,and hints at that the DD^(*)molecular and non-molecular components are of similar importance in the Z_(c)(3900)formation.

关键词： exotic hadrons dispersion relations final state interaction triangle singularity

Impact of the antiproliferative agent ciclopirox olamine treatment on stem cells proteome

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World Journal of Stem Cells 2013年第1期5卷 9-25页

作者： Gry H Dihazi Asima Bibi Olaf Jahn Jessica Nolte Gerhard A Mueller Wolfgang Engel Hassan Dihazi Department of Nephrology and Rheumatology Georg-August University GoettingenD-37075 GoettingenGermany Proteomics Group Max-Planck-Institute of Experimental MedicineD-37075 GoettingenGermany deutsche forschungsgemeinschaft research center for molecular physiology of the brain D-37073 Institute of Human Genetics Georg-August University GoettingenD-37073 GoettingenGermany

AIM:To investigate the proteome changes of stem cells due to ciclopirox olamine (CPX) treatment compared to control and retinoic acid treated cells. METHODS:Stem cells (SCs) are cells, which have the ability to continuously divide and differentiate into various other kinds of cells. Murine embryonic stem cells (ESCs) and multipotent adult germline stem cells (maG-SCs) were treated with CPX, which has been shown to have an antiproliferative effect on stem cells, and compared to stem cells treated with retinoic acid (RA),which is known to have a differentiating effect on stem cells. Classical proteomic techniques like 2-D gel electrophoresis and differential in-gel electrophoresis (DIGE) were used to generate 2D protein maps from stem cells treated with RA or CPX as well as from non-treated stem cells. The resulting 2D gels were scanned and the digitalized images were collated with the help of Delta 2D software. The differentially expressed proteins were analyzed by a MALDI-TOF-TOF mass spectrometer, and the identified proteins were investigated and categorized using bioinformatics. RESULTS:Treatment of stem cells with CPX, a synthetic antifungal clinically used to treat superficial mycoses, resulted in an antiproliferative effect in vitro, without impairment of pluripotency. To understand the mechanisms induced by CPX treatments which results in arrest of cell cycle without any marked effect on pluripotency, a comparative proteomics study was conducted. The obtained data revealed that the CPX impact on cell proliferation was accompanied with a significant alteration in stem cell proteome. By peptide mass fingerprinting and tandem mass spectrometry combined with searches of protein sequence databases, a set of 316 proteins was identified, corresponding to a library of 125 non-redundant proteins. With proteomic analysis of ESCs and maGSCs treated with CPX and RA, we could identify more than 90 single proteins, which were differently expressed in both cell lines. We cou

关键词： Stem cells Differentiation Hypusination Ciclopirox olamine Proteomics Retinoic acid

Engineering a customized nanodrug delivery system at the cellular level for targeted cancer therapy

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Science China Chemistry 2018年第4期61卷 497-504页

作者： Jin Li Liping Qiu Sitao Xie Jing Zhang Liqin Zhang Honglin Liu Juan Li Xiaobing Zhang Weihong Tan molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of Life SciencesAptamer Engineering Center of Hunan ProvinceHunan University center for research at Bio/Nano Interface Department of Chemistry and Department of Physiology and Functional GenomicsHealth Cancer CenterUF Genetics Institute and McKnight Brain InstituteUniversity of Florida key Laboratory for Analytical Science of Food Safety and Biology (Ministry of Education) College of ChemistryFuzhou University

Drug administration customized to individual cells could intrinsically address cancer heterogeneity and provide a safe and effective method for delivering personalized treatment. To accomplish this, we developed a smart nanodrug delivery system characterized by cancer cell-targeted drug delivery and intracellular biomarker-responsive drug activation. This system was composed of a long-nicked DNA duplex formed by tandem hybridization of two extended antisense oligonucleotides whose ends were separately blocked with a cancer cell-specific aptamer, AS1411,and a replaceable anti-biomarker probe(ABP). We demonstrated that this DNA nanodrug was directed to cancer cells with the guidance power of AS 1411 and then activated by the presence of a given intracellular biomarker. By using such a belt-and-braces strategy, this DNA nanodrug system could safely and efficiently accelerate apoptosis of target cancer cells. Moreover, since the expression level of biomarkers tends to indicate the specific physiological state of individual cells, biomarker-responsive activation of the nanodrug is expected to enable customized drug administration at the cellular level.

关键词： aptamer drug delivery personalized medicine antisense oligonucleotides

Self-assembled multifunctional DNA nanoflowers for the circumvention of multidrug resistance in targeted anticancer drug delivery

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Nano research 2015年第11期8卷 3447-3460页

作者： Lei Mei Guizhi Zhu Liping Qiu Cuichen Wu Huapei Chen Hao Liang Sena Cansiz Yifan Lv Xiaobing Zhang Weihong Tan molecular Sciences and Biomedicine Laboratory State Key Laboratory for Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering College of Biology Collaborative Innovation Center for Chemistry and Molecular Medicine Hunan University Changsha 410082 China Departments of Chemistry Physiology and Functional Genomics Center for Research at the Bio/Nano Interface Shands Cancer Center UF Genetics Institute and McKnight Brain Institute University of Florida Gainesville FL 32611-7200 USA Laboratory of molecular Imaging and Nanomedicine National Institute of Biomedical Imaging and Bioengineering National Institutes of Health Bethesda MD 20892 USA

Cancer chemotherapy has been limited by its side effects and multidrug resistance （MDR）, the latter of which is partially caused by drug efflux from cancer cells. Thus, targeted drug delivery systems that can circumvent MDR are needed. Here, we report multifunctional DNA nanoflowers （NFs） for targeted drug delivery to both chemosensitive and MDR cancer cells that circumvented MDR in both leukemia and breast cancer cell models. NFs are self-assembled via potential co-precipitation of DNA and magnesium pyrophosphate generated by rolling circle replication, during which NFs are incorporated using aptamers for specific cancer cell recognition, fluorophores for bioimaging, and doxorubicin （Dox）- binding DNA for drug delivery. NF sizes are tunable （down to N200 nm in diameter）, and the densely packed drug-binding motifs and porous intrastructures endow NFs with a high drug-loading capacity （71.4%, wt/wt）. Although the Dox- loaded NFs （NF-Dox） are stable at physiological pH, drug release is facilitated under acidic or basic conditions. NFs deliver Dox into target chemosensitive and MDR cancer cells, preventing drug efflux and enhancing drug retention in MDR cells. NF-Dox induces potent cytotoxicity in both target chemosensitive cells and MDR cells, but not in nontarget cells, thus concurrently circumventing MDR and reducing side effects. Overall, these NFs are promising tools for circumventing MDR in targeted cancer therapy.

关键词： aptamer rolling circle replication self-assembly DNA nanotechnology multidrug resistance targeted cancer therapy

Transcriptional regulation of wheat VER2 promoter in rice in response to abscisic acid,jasmonate,and light

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Journal of Genetics and Genomics 2009年第6期36卷 371-377页

作者： Hong Feng Wen-Zhong Xu Hong-Hui Lin Kang Chong College of Life Science Sichuan University Chendu 610064 China research center of molecular and Developmental Biology Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Sichuan College of Education Chengdu 610041 China

VER2 is a key gene associated with vernalization process in winter wheat. The expression of VER2 can be induced by low temperature treatment. To further understand how the expression of this gene is mediated by various external and internal factors, different lengths of the VER2 promoter region have been transcriptionally fused with a reporter gene, green fluorescence protein （GFP）, and transformed into the model plant, rice （Oryza sativa L.）. Using confocal and Western blot analyses, we determined several possible response elements in the promoter region, which could sense ABA, JA, and other environmental cues.

关键词： VER2 promoter ABA JA

TIR enzymatic functions:signaling molecules and receptor mechanisms

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aBIOTECH 2023年第2期4卷 172-175页

作者： Li Wan National Key Laboratory of Plant molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai200032China

The evolutionarily conserved Toll/Interleukin-1 Receptor(TIR)domains across kingdoms of prokaryotes,plants,and animals play critical roles in innate *** studies have revealed the enzymatic functions of TIRs,the structural bases of TIRs as holoenzymes,and the identity of TIR-generated small signaling molecules and their receptors,which significantly advanced our understanding on TIR-mediated immune signaling *** reviewed the most up-to-date findings in TIR enzymatic functions from the perspectives of signaling molecules and receptor mechanisms.

关键词： NLR immune receptors TIR domains Signaling molecules Cell death

WUSCHEL: the versatile protein in the shoot apical meristem

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Science China(Life Sciences) 2021年第1期64卷 177-178页

作者： Lin Xu National Key Laboratory of Plant molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai200032China

In vascular plants, almost all above-ground organs are derived from the shoot apical meristem (SAM). The developmental role of the SAM has been extensively studied,especially in the model dicot plant Arabidopsis thali... 详细信息

关键词： versatile organs

Differentiation renders susceptibility to excitotoxicity in HT22 neurons

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Neural Regeneration research 2013年第14期8卷 1297-1306页

作者： Minchao He Jun Liu haowu Cheng Yigang Xing William Z Suo Department of Neurology Sun Yat-sen Memorial HospitalSun Yat-sen University Laboratory for Alzheimer's Disease & Aging research Veterans Affairs Medical Center Department of Neurology University of Kansas Medical Center molecular & Integrative physiology University of Kansas Medical Center

HT22 is an immortalized mouse hippocampal neuronal cell line that does not express cholinergic and glutamate receptors like mature hippocampal neurons in vivo. This in part prevents its use as a model for mature hippocampal neurons in memory-related studies. We now report that HT22 cells were appropriately induced to differentiate and possess properties similar to those of mature hippocampal neurons in vivo, such as becoming more glutamate-receptive and excitatory. Results showed that sensitivity of HT22 cells to glutamate-induced toxicity changed dramatically when comparing undifferentiated with differentiated cells, with the half-effective concentration for differentiated cells reducing approximately two orders of magnitude. Moreover, glutamate-induced toxicity in differentiated cells, but not undifferentiated cells, was inhibited by the N-methyi-D- aspartate receptor antagonists MK-801 and memantine. Evidently, differentiated HT22 cells expressed N-methyI-D-aspartate receptors, while undifferentiated cells did not. Our experimental findings indicated that differentiation is important for immortalized cell lines to render post-mitotic neuronal properties, and that differentiated HT22 neurons represent a better model of hippocampal neurons than undifferentiated cells.

关键词： neural regeneration brain injury HT22 differentiation N-methyI-D-aspartate receptor glutamateexcitatory neurotoxicity mitosis hippocampus neurons MK-801" memantine grants-supportedpaper neuroregeneration

Patterning inconsistencies restrict the true potential of dopaminergic neurons derived from human induced pluripotent stem cells

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Neural Regeneration research 2021年第4期16卷 692-693页

作者： Sameehan Mahajani Mathias Bähr Sebastian Kügler Department of Neurology University Medical Center GöttingenGöttingenGermany center for Nanoscale Microscopy and molecular physiology of the brain at Department of Neurology University Medical Center GöttingenGöttingenGermany Department of Pathology Stanford University School of MedicineStanfordCAUSA

Human induced pluripotent stem cells(hiPSCs)are multipotent stem cells genetically reprogrammed using transcription factors,such as Sox2,c-Myc,Oct3/4 and Klf4(Takahashi and Yamanaka,2006)from fibroblasts,derived from either patient or control *** factors are highly expressed in embryonic stem cells,and their overexpression can induce pluripotency in human somatic cells such as *** the generation of hiPSCs after reprogramming,these cells can be further differentiated into multiple neuronal cell types by using a strictly designed *** process is known as *** use of these hiPSCs derived neurons holds immense potential for researchers to uncover the underpinnings of disease pathophysiology and therefore is considered as a powerful tool.

关键词： potential restrict holds