检索结果-南通市图书馆

Most common SLC25A13 mutation in 400 Chinese infants with intrahepatic cholestasis

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第18期16卷 2278-2282页

作者： Fu, Hai-Yan Zhang, Shao-Ren Yu, Hui Wang, Xiao-Hong Zhu, Qi-Rong Wang, Jian-She Fudan Univ Childrens Hosp Ctr Pediat Liver Dis Shanghai 201102 Peoples R China Fudan Univ Shanghai Med Coll Dept Pediat Shanghai 201102 Peoples R China

AIM:To establish the real time fluorescence polymerase chain reaction(RT-PCR) with dual labeled probes for fast detection of SLC25A13 gene mutation ***:Four hundred infants(< 1 year of age) with unexplained intrahepatic cholestasis from 18 provinces or municipalities in China were enrolled in this study for detecting their SLC25A13 gene mutation *** primers and fluorescence-labeled probes for detecting SLC25A13 gene mutation 841del4 were *** and mutant sequences were detected by PCR with two fluorescence-labeled *** a single RT-PCR,results were obtained by analyzing the take-off ***-four positive and 14 negative samples were retested by direct ***:Eight homozygous and 30 heterozygous mutations were detected in 46 mutant alleles with a 851del4 mutation rate of 5.8%(46/800).Twenty-six and 20 mutant alleles were observed respectively,in 474 and 242 alleles from the intermediate and southern areas of *** mutant allele was detected in 84 alleles from northern ***-four positive samples including 4 homozygous and 20 heterozygous mutations,and 14 negative samples were retested by direct sequencing,which confirmed that the accuracy of RTPCR was 100%.CONCLUSION:RT-PCR can detect the mutation 851del4 in infants with intrahepatic cholestasis with an accuracy of 100%.

关键词： 851del4 mutation Neonatal intrahepatic cholestasis Real-time fluorescent polymerase chain reaction SLC25A13 gene

Seroepidemiology of Helicobacter pylori infection among asymptomatic Chinese children

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2000年第5期6卷 759-761页

作者： Xu CD Chen SN Jiang SH Xu JY Shanghai Second Mil Med Univ Ruijin Hosp Dept Pediat Shanghai 200025 Peoples R China

INTRODUCTIONIncreasing data has demonstrated that Helicobacterpylori(***),a spiral gram negativebacterium,colonized in human stomach,can causetype B gastritis,is strongly associated withgastric and duodenal ulceration,and has beenimplicated in the causation of gastric carcinomaand mucosa-associated lymphoid tissue(MALT)*** has been reported that there

关键词： Helicobacter infection/infancy children Helicobacter pylori seroepidemiology gastritis stomach neoplasms gastric mucosa family

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

儿童外伤性脑损伤后血浆中总镁和镁离子浓度

世界核心医学期刊文摘（儿科学分册） 2005年第7期1卷 52-53页

作者： Mendez D.R. Corbett R. Macias C. Laptook A. 虎小毅 Baylor College of medicine Dept.of Pediat.Emergency Medicine 6211 Fannin Street Houston TX 77030 United States Dr.

This study examined 1) whether plasma total Mg (TMg) and ionized Mg (IMg) concentrations in children are reduced by traumatic brain injury (TBI) and 2) whether the extent of reduction correlates with severity of trauma assessed by the Glasgow Coma Scale (GSC) *** was a prospective cohort study of 98 pediatric patients who had TBI and were admitted through the emergency department.A GCS score was assigned and blood was obtained upon presentation and 24 h *** was analyzed for TMg and *** were grouped into three categories -GCS scores 13-15, 8-12, and<8 -to designate mild (M = 21), moderate (M = 37), and severe (M = 40) TBI, *** was obtained from 50 healthy children before elective surgery as *** subjects had a TMg and an IMg of 0.94 ±0.08 and 0.550 ±0.06 *** patients had an initial TMg and IMg of 0.83 ±0.09 and 0.520 ±0.05 mM, *** TMg for mild, moderate, and severe TBI subgroups (0.87 ±0.16, 0.81 ±0.15, and 0.83 ±0.14 mM, respectively) was reduced from control subjects (p < 0.01).IMg was reduced only in the severe TBI subgroup (0.516 ±0.07 mM; p = 0.016).Twenty-four hours later, TMg remained lower than in control subjects for all subgroups of TBI; however, IMg *** in children is associated with a reduction in TMg, whereas IMg decreased only with severe *** returned to control values by 24 h despite a continued lower TMg, suggesting mechanisms to maintain *** in plasma IMg may serve as a marker for TBI but only over a limited time interval.

关键词：脑损伤镁离子浓度创伤严重程度急诊科前瞻性队列研究外科手术

Treatment of hepatitis B virus-associated glomerulonephritis:A meta-analysis

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第6期16卷 770-777页

作者： Zhang, Yu Zhou, Jian-Hua Yin, Xiao-Ling Wang, Feng-Yu Huazhong Univ Sci & Technol Dept Pediat Tongji Hosp Tongji Med Coll Wuhan 430030 Hubei Province Peoples R China

AIM:To evaluate the efficacy of antiviral or corticosteroid treatment on hepatitis B virus-associated glomerulonephritis(HBV-GN) . METHODS:Six and five trials were used respectively to evaluate the efficacy of either antiviral or corticosteroid treatment on *** patients were pooled separately to assess their response to the above treatment *** primary and secondary outcomes were remission of proteinuria and clearance of Hepatitis B e-antigen(HBeAg) ,respectively.A fixed or random effect model was established to collect the data. RESULTS:The remission rate of proteinuria(RR=1.69,95%CI:1.08-2.65) and the clearance rate of HBeAg(RR =6.44,95%CI:3.11-13.35) were significantly higher in antiviral treatment group than in control *** proteinuria remission was significantly associated with HBeAg clearance(P=0.002) .However,the difference in proteinuria remission rate was not statistically significant between corticosteroid treatment group and controlgroup(RR=1.45,95%CI:0.68-3.11) .Antiviral therapy could significantly promote the HBeAg clearance in pediatric patients,but neither antiviral nor corticosteroid therapy could significantly decrease proteinuria in pediatric patients compared to controls. CONCLUSION:Antiviral but not corticosteroid treatment can decrease proteinuria and promote HBeAg clearance in HBV-GN patients.

关键词： Hepatitis B virus-associated glomerulonephritis Drug therapy Meta-analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

到急诊科就诊的儿科晕厥患者

世界核心医学期刊文摘（儿科学分册） 2005年第1期1卷 42-42页

作者： Massin M.M. Bourguignont A. Coremans C. 郭战宏 dept.of pediat.and-Math.InstituteUniversity of Li' ege Li' ege Belgium

目的:为了评价到西欧某一急诊科就诊的非选择性儿童晕厥的流行病学特点。研究设计:分析了在笔者急诊科见到的因晕厥事件就诊的连续的226例儿科患者的晕厥原因和检查。结果:神经心脏性晕厥和神经紊乱是最常见的诊断(分别为80%和9%),其他... 详细信息

目的:为了评价到西欧某一急诊科就诊的非选择性儿童晕厥的流行病学特点。研究设计:分析了在笔者急诊科见到的因晕厥事件就诊的连续的226例儿科患者的晕厥原因和检查。结果:神经心脏性晕厥和神经紊乱是最常见的诊断(分别为80%和9%),其他原因包括心理、心脏、呼吸、中毒和代谢异常。神经心脏性晕厥和与疾病有关的晕厥依靠病史和体检就很容易确诊或排除。有132例(58%)未进行心电图检查。大多数怀疑有神经心脏性晕厥的患者都进行了脑电图检查,其中29%的患者住院治疗。心脏异常有5例(2%),其中2例在早期被误诊。结论:在儿童期,多种原因均可引起晕厥,结果在目标导向的习惯下一个不能解决此问题的评估将是昂贵、耗时及对有关各方都是一个打击。通常情况下详细的病史和彻底的体检对指导全科医师为特定患者选择诊断性检查项目很有必要。

关键词：急诊科诊断性检查儿科患者神经紊乱全科医师住院治疗研究设计 admitted fashion referred

Expression of NF-kappaB in rotavirus-induced damage to the liver and biliary tract in neonatal mice

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2007年第2期6卷 188-193页

作者： Huang, Lei Gu, Wei-Zhong Si, Xin-Min Wei, Ming-Fa Feng, Jie-Xiong Huazhong Univ Sci & Technol Dept Pediat Surg Tongji Hosp Wuhan 430030 Peoples R China Zhejiang Univ Sch Med Dept Pathol Childrens Hosp Hangzhou 310003 Peoples R China Wuhan Univ Renmin Hosp Dept Gastroenterol Wuhan 430060 Peoples R China

BACKGROUND: Biliary atresia, the etiology of which still remains unclear, occurs exclusively in newborns and most are infected with rotavirus. In this study, we aimed to investigate the histopathological patterns of different kinds of rotavirus in the liver and biliary tract of neonatal mice and the expression of NF-kappa B in the liver and biliary tract of infected mice. METHODS: Twenty-three adult mice (8 were male and 15 female) were divided into 8 breeding pairs, and each pair (I male and 2 females) was housed in a cage in a laminar flow hood. Newborn mice, 24-48 hours old were randomly divided into A, B and C groups. The A and B groups were respectively inoculated with MMU18006 and SA11 rotavirus through the intraperitoneal route, while group C as blank control was only inoculated with culture medium. The liver was dissected after 5, 10, 15, 21 and 28 days; the weight of each mouse and the histopathological patterns in the liver were recorded. The expression of NF-kappa B in the liver and intrahepatic bile ducts was detected by immunohistochemical staining and the expression intensity was analyzed with a GT-2 imaging instrument. RESULTS: The average increase in weight of infected mice was significantly slower than that of the normal control, while the growth rate of group A (injected with MMU18006 rotavirus) was slower than that of group B (SA11 rotavirus). In infected mice, the acute and chronic inflammation of liver and intra- and extra-hepatic bile ducts was more significant in group A. Stenosis was found in most intrahepatic bile ducts, and sporadically in extrahepatic bile ducts. The expression of NF-kappa B in infected mice was dramatically higher than that of the normal control, while the expression in group A was higher than in group B. CONCLUSIONS: Significant damage to the liver and biliary tract of neonatal mice can be induced by inoculating MMU18006 rotavirus through the intraperitoneal route, which is very similar to the pathology of biliary atresi

关键词： rotavirus biliary atresia NF-kappa B

Study of clinical outcome and prognosis in pediatric core binding factor-acute myeloid leukemia

在线全文

学校读者我要写书评

暂无评论

China Medical Abstracts(Internal medicine) 2019年第3期36卷 181-182页

作者： WU Jun dept pediat People’s HospPeking Univ

Objective To analyze the clinical outcome and the prognostic factor in pediatric patients with core binding factor-acute myeloid leukemia(CBF-AML).Methods A total of 121 newly diagnosed pediatric CBF-AML patients enrolled from Aug.2005 to Sep.2017 were retrospectively *** incidence of relapse(CIR),event-free survival(EFS)and overall survival(OS)rates were estimated by Kaplan-Meier method and prognostic factors were evaluated by Cox regression with *** Of the 121 patients,120 patients were assessed for bone marrow remission after induction chemotherapy.100 cases(83.3%)achieved complete remission(CR)after the first course of chemotherapy.119 cases(99.2%)achieved CR after the second course of *** the 121 patients,13 patients(10.7%)had recurrence with the median interval of recurrence as 13.8 months(3.7 to 58.8 months).17 patients(14.0%)*** CIR,EFS and OS at 3 years were 12.7%,77.5%and 82.8%,*** factors including age at diagnosis,sex,initial WBC count,presence of extramedullary leukemia,C-KIT expression,additional chromosomal abnormalities,and CR after the first course of chemotherapy were analyzed by multivariate regression analysis of *** analysis identified that additional chromosomal abnormalities was the only independent risk factor affecting OS(HR=4.289,95%CI 1.070-17.183,P=0.040).Conclusion pediatric CBF-AML was a unique setting of prognostic *** produced good *** chromosomal abnormalities was the only independent risk factor for OS in pediatric CBF-AML.

关键词： pediatric chemotherapy clinical

Abdominal pain among children re-evaluation of a diagnostic algorithm

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2002年第5期8卷 947-951页

作者： Zhou, H Chen, YC Zhang, JZ Capital Univ Med Sci Beijing Childrens Hosp Dept Pediat Surg Beijing 100045 Peoples R China

AIM: To re-evaluate the algorithm that has been used forover 40 years for diagnosis of acute abdominal pain ***: Among the 937 cases admitted to the surgicalemergency ward in 2000, 656 cases of acute appendicitiswere studied to evaluate the usefulness of the presentalgorithm for its colculated accuracy, false positive and falsenegative rates, the sensitivity and specificity in the instantdiagnosis of various types of acute appendicitis in differentage *** algorithm used was established in 1958and revisedror this study in 1999. It includes a 3-step analysisof clinical presentations, i.e.: firstly, a diagnosis of surgicalpain by definite organic abdominal signs; then a diagnosisof the subgroup of surgical condition by special signs; andfinally the diagnosis of the present disease by specific signs.A footnote describes a 'comparative technique″ of abdominalexamination in non-cooperative ***: The general accuracy of diagnosis was 92.8 %,overall mortality 0.1% among 973 cases of abdominal painin 2000. 373 attending surgeons and 241 residents includingtrainees joined the diagnosis and treatment with noremarkable difference in the results. The incidence of acuteappendicitis, 656 in 973 cases, was 67.4 % representingthe majority of abdominal pain. In the series of 656 cases,the accuracy of diagnosis of acute appendidtis was 93.6 %,false positive 6.4 %, false negative 0.9 %, sensitivity at firstvisit 82.7 %, specificity for appendicitis 98.0 %, no death ordocumentary ***: The present algorithm used for diagnosisof acute abdominal pain is effective and preferable inreducing misdiagnosis and maltreatment at emergency. Theuse of some modern technology should be further explored.

关键词：儿童腹痛鉴别诊断病因

Experimental study on the effect of Kang-Lai-Te induced apoptosis of human hepatoma carcinoma cell HepG2

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2009年第3期8卷 267-272页

作者： Lu, Yun Wu, Li-Qun Dong, Qian Li, Chang-Sheng Qingdao Univ Dept Hepatobiliary Surg Affiliated Hosp Coll Med Qingdao 266003 Peoples R China Qingdao Univ Dept Pediat Surg Affiliated Hosp Coll Med Qingdao 266003 Peoples R China Qingdao Univ Ctr Cell & Mol Pathol Affiliated Hosp Coll Med Qingdao 266003 Peoples R China

BACKGROUND: Kang-Lai-Te (KLT) is extracted from the traditional Chinese herbal medicine Semen Coicis, which has been used in China as an effective clinical drug for over a thousand years. It contains numerous ingredients with anti-tumor effects. In our previous studies on transplanted hepatomas in rats, KLT could stop the cells in the G2+M stage of cell cycle and then reduce the number of cells entering the stage G0 and G1, but the mechanism of the anti-proliferative effect was unknown. In this experiment, we examined whether KLT inhibits HepG2 cell growth, if so, tried to explore its mechanism. METHODS: KLT at different concentrations was used for the treatment of hepatocellular carcinoma cells in vitro, respectively. The proliferation inhibitory rate was evaluated by MTT assay, induction of cell apoptosis rate and the protein levels of Fas and Fas ligand (FasL) were determined by flow cytometry (FCM), and the expression of Fas and FasL mRNA was detected by real-time fluorescent quantitative RT-PCR. RESULTS: KLT produced an obvious time and dose-dependent inhibitory effect on HepG2 cells, and marked apoptosis was detected by FCM The protein of Fas increased by 11.01%, 18.71%, 28.71% and 37.15%; the protein of FasL increased by 1.49%, 1.91%, 3.27% and 3.38% in comparison with the control (P<0.05). Real-time fluorescent quantitative RT-PCR showed that treating HepG2 cells with KLT caused the upregulation of Fas and FasL mRNA. CONCLUSION: KLT inhibits HepG2 growth by inducing apoptosis, which may be mediated through activation of the Fas/FasL pathway. (Hepatobiliary Pancreat Dis Int 2009; 8: 267-272)

关键词： Kang-Lai-Te drugs, Chinese herbal hepatocellular carcinoma HepG2 Fas/FasL