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Cerebrovascularβ-dystroglycan immunoreactivity in vertebrates:not detected in anurans and in the teleosts Ostariophysi and Euteleostei

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Integrative Zoology 2020年第1期15卷 16-31页

作者： Mihály KÁLMÁN David L.LŐRINCZ Olivér M.SEBŐK Csilla ARI Erzsébet OSZWALD Hiroaki SOMIYA Veronika JANCSIK Department of Anatomy Histology and EmbryologySemmelweis UniversityBudapestHungary University of Leicester Dept.of NeurosciencePsychology and BehaviourLeicesterUnited Kingdom Hyperbaric neuroscience Research Lab. Dept of PsychologyUniversity of South FloridaTampaFloridaUSA Department of Environmental Biology Chubu UniversityChubuJapan Department of Anatomy Veterinary UniversityBudapestHungary

The aim of the present paper was to check for the presence of cerebrovascular dystroglycan in vertebrates,because dystroglycan,which is localized in the vascular astroglial end-feet,has a pivotal function in glio-vascular *** mammalian brains,the immunoreactivity ofβ-dystroglycan subunit delineates the *** results of the present study demonstrate similar patterns in other vertebrates,except for anurans and the teleost groups Ostariophysi and *** this study,we investigated 1 or 2 representative species of the main groups of Chondrichthyes,teleost and non-teleost ray-finned fishes,urodeles,anurans,and *** also investigated 5 mammalian and 3 bird *** were obtained from breeders or *** presence ofβ-dystroglycan was investigated immunohistochemically in free-floating ***-embedding electron microscopical immunohistochemistry on Heterodontus japonicus shark brains demonstrated that in Elasmobranchii,β-dystroglycan is also localized in the perivascular glial end-feet despite the different construction of their blood-brain *** results indicated that the cerebrovascularβ-dystroglycan immunoreactivity disappeared separately in anurans,and in teleosts,in the latter group before its division to Ostariophysi and ***munohistochemistry in muscles and western blots from brain homogenates,however,detected the presence ofβ-dystroglycan,even in anurans and all teleosts.A possible explanation is that in the glial end-feet,β-dystroglycan is masked in these animals,or disappeared during adaptation to the freshwater habitat.

关键词： anurans cerebrovascular dystroglycan elasmobranchs teleosts

Inappropriate Gold Standard Bias In Cervical Cancer Screening Studies

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Inappropriate Gold Standard Bias In Cervical Cancer Screenin...

尼莫地平对局灶性脑缺血诱导的成年小鼠齿状回神经元再生的影响

24th International Papillomavirus Conference and Clinical Workshop

作者： Robert Pretorius Jerome Belinson Raoul Burchette Jennifer Smith dept Ob/Gyn Southern California Permanente Medical Group-FontanaFontanaCA92335USA dept Ob/GynThe Cleveland Clinic FoundationClevelandOH44195USA dept.Research and Evaluation Southern California Permanente Medical GroupLosRoblesCAUSA dept.Epidemiology Univ.North CarolinaChapelHillNC27599USA

＜正＞*** acid aided visual inspection（VIA）and colposcopic-directed biopsy both miss small ≥ cervical intraepithelial neoplasia（CIN） *** object of this review is to determine whether inflation of sensitivity of VIA occurs when colposcopic- directed biopsy is the gold standard for ≥ CIN 2. *** reviewed 375 women with ≥ CIN 2 from the Shanxi Province Cervical Cancer Screening Study Ⅱ.These women

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中国药理学通报 2005年第4期21卷 407-412页

作者：罗春霞张爱霞王玮 LIU Shuhong 孙婧朱新建张书刚 LIU Youming 朱东亚南京医科大学药学院药理学教研室江苏南京210029 dept.of Physiology and Biophysics Neuroscience Research Group Faculty of Medicine University of Calgary Calgary T2N4N1Canada

目的　研究L 型电压门控式Ca2+通道(L VGCC)阻断对局灶性脑缺血诱导成年动物海马齿状回神经元再生的影响。方法　成年♂C57 /BL/6小鼠行90min大脑中动脉阻塞(MCAO)手术或者假手术,进行以下三个实验:①手术后48h处死动物,经TTC染色,观察... 详细信息

目的　研究L 型电压门控式Ca2+通道(L VGCC)阻断对局灶性脑缺血诱导成年动物海马齿状回神经元再生的影响。方法　成年♂C57 /BL/6小鼠行90min大脑中动脉阻塞(MCAO)手术或者假手术,进行以下三个实验:①手术后48h处死动物,经TTC染色,观察尼莫地平对梗死面积的影响;②手术后5 ~7d,动物经腹腔注射5 溴脱氧尿苷(Br dU),末次注射后24h处死动物,通过免疫组化、Fluoro Jade染色检测尼莫地平对脑缺血诱导的齿状回细胞增殖的作用,并确定细胞增殖是否依赖于海马齿状回细胞的死亡;③动物接受如前所述的BrdU注射,并于末次注射后4wk处死动物,采用免疫荧光双标记观察尼莫地平对脑缺血后新生细胞表型分化的影响。结果　小鼠经过90minMCAO后,齿状回新生神经元数目明显增多,这种缺血诱导的神经元再生并不依赖于齿状回神经细胞的死亡,采用尼莫地平阻断L VGCC则抑制了缺血后的神经元再生。结论　脑缺血后Ca2+经L VGCC内流直接促进缺血诱导的神经元再生。

关键词：局灶性脑缺血尼莫地平神经元再生 L-型电压门控式Ca^2+通道

c-fos,bax和p53的反义寡聚核苷酸(ASOs)对溶血磷脂酸所致的培养小鼠大脑皮层神经元凋亡的影响(英文)

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山西医科大学学报 2009年第9期40卷 778-785页

作者：郑肇青方羡君胥显民李建国乔健天山西医科大学神经生物学研究室太原030001 neuroscience group Division of Basic Biomedical SciencesSchool of MedicineUniversity of South Dakota dept.of Biochemistry Virginia Commonwealth University

溶血磷脂酸(lysophosphatidic acid,LPA)在高浓度时( >50μmol/L)显示出对培养大脑皮层神经元致凋亡特性。本研究旨在初步分析溶血磷脂酸致凋亡过程中对相关基因表达的依赖性,在研究中结合琼脂糖电泳,HO33342染色和TUNEL技术观察c-fos,... 详细信息

溶血磷脂酸(lysophosphatidic acid,LPA)在高浓度时( >50μmol/L)显示出对培养大脑皮层神经元致凋亡特性。本研究旨在初步分析溶血磷脂酸致凋亡过程中对相关基因表达的依赖性,在研究中结合琼脂糖电泳,HO33342染色和TUNEL技术观察c-fos,bax和p53三种反义寡聚核苷酸对溶血磷脂酸致培养大脑皮层神经元致凋亡的影响。结果显示结合c-fos, bax和p53三种反义寡聚核苷酸均表现出对溶血磷脂酸致凋亡的神经保护作用。溶血磷脂酸的致凋亡过程为c-fos,bax和p53基因依赖性,不同于b淀粉样蛋白片段31 -35的致凋亡特性。

关键词：溶血磷脂酸凋亡 c-fos bax p53 反义寡聚核苷酸细胞培养大脑皮层神经元

对3岁儿童进行视觉筛选可行吗?比较Lea Sym-bol表和HVOT(LM)表

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世界核心医学期刊文摘（眼科学分册） 2005年第8期1卷 15-15页

作者： (LM) chart Kvarnstrm G. Jakobsson P. 潘佳鸿 dept. of neuroscience and Locomotion Division of Ophthalmology oping University SE-581 85 LinkLinkping Sweden

Purpose: The aim of this prospective study was to compare visual screening at the age of 3 years with screening at 4 years using two different charts. Methods : A total of 478 3-year-old children were tested at four child health care cen tres (CHCCs). Of these children, 440 were tested again at the age of 4 years. A third group, a control group, consisting of 229 children, was examined only at t he age of 4 years. All children were tested with both the HVOT chart and the Lea Symbol chart. Results: Testability rates for 3-year-olds were almost the same with the Lea Symbol chart and the HVOT chart (82.8%and 84.8%, respectively). The corresponding rates for the same children tested at 4 years of age were 96.5 %and 97.0%, and for the 4-year-olds not previously tested 92.9%and 92.8%. The mean testing time was somewhat shorter for the Lea Symbol chart in all three groups, but the difference was not statistically significant. The difference in the assessment of visual acuity between the two charts was small and less than 1/10th of a line. The positive predictive value was lower at 3 years (58%) than has previously been found at 4 years (74.6%). Conclusion: Three-year-old chi ldren co-operate well in visual acuity testing. However, the examination time i s a little longer and the testability rate is about 10%lower than at 4 years. B oth 3-year-old and 4-year-old children can be tested equally well with the H VOT and the Lea Symbol charts.

关键词： HVOT LM Lea Sym-bol 易测测试时间保健中心阳性预测值

结合临床风险因素和血清胆红素水平来预测新生儿高胆红素血症

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世界核心医学期刊文摘（儿科学分册） 2005年第6期1卷 26-26页

作者： Newman T. B. Liljestrand P. Escobar G. J. 王一飞 dept. of Epidemiol. and Biostatist. Univ. of California San Francisco Box 0560 San Francisco CA 94143 United States Dr.

Objectives: (1) To validate a previously reported risk index for predicting total serum bilirubin (TSB) levels of 25 mg/dL (428 μmol/L) or higher; (2) to combine a subset of this index with TSB levels measured at less than 48 hours to predict subsequent TSB levels of 20 mg/dL (342 μmol/L) or higher. Design: Nested case-control study using electronic and paper records (study 1). Retrospective cohort study using electronic records only (study 2). Setting: Northern California Kaiser permanente hospitals. Patients: Subjects for both studies were newborns weighing 2000 g or more and of 36 weeks’or more gestation. The validation study included 67 cases born 1997-1998 who developed TSB levels of 25 mg/dL or higher at less than 30 days and 208 randomly selected control subjects. Subjects for study 2 were 5706 newborns who both were discharged from the hospital and had a TSB level measured at less than 48 hours. Results: The risk index performed similarly in the validation group, born in 1997-1998, and the derivation group, born in 1995-1996 (area under the receiver operating characteristic curve = 0.83 vs 0.84). Of the 5706 newborns with TSB levels measured before 48 hours, 270 (4.7%) developed a TSB level of 20 mg/dL or higher. Of these, 254 (94%) had a TSB level at the 75th percentile or higher at less than 48 hours. The risk index improved prediction over the TSB level alone, largely owing to the effect of gestational age. For example, for those with a TSB level at the 95th percentile or higher at less than 48 hours, the risk increased from 9%for newborns born at 40 weeks’or more gestation to 42%for those born at 36 weeks. Conclusion: Clinical risk factors significantly improve prediction of subsequent hyperbilirubinemia compared with early TSB levels alone, especially in those with early TSB levels above the 75th percentile.

关键词：临床风险出生后风险指数验证研究百分位数仪器记录回顾性队列研究妊娠时间病例对照研究电子仪器