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Journal of Integrative Agriculture 2018年第9期17卷 2042-2053页

作者： ZHENG Na ZHANG Liu-ping GE Feng-yong HUANG Wen-kun KONG Ling-an PENG De-liang LIU Shi-ming Institute of plant protection Chinese Academy of Agricultural Sciences College of plant protection Hunan Agricultural University

Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far. Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone. We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis. Of them, except for one isolate that showed diversity of virulence to different soybeans （virulent to one soybean whereas avirulent to another soybean）, the others were all virulent to the two tested soybeans： both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×10^6 conidia mL-1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted. Finally, we developed the specific cleaved amplified polymorphic sequences （CAPS） markers to easily differentiate Fusarium isolates. The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean.

关键词： Fusarium soybean root rot conidia secretions virulence cleaved amplified polymorphic sequences (CAPS) marker

Assessment of Rice Cultivars in China for Field Resistance to Aphelenchoides besseyi

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Journal of Integrative Agriculture 2014年第10期13卷 2221-2228页

作者： FENG Hui WEI Li-hui LIN Mao-song ZHOU Yi-jun College of plant protection Nanjing Agricultural University Institute of plant protection Jiangsu Academy of Agricultural Sciences

The effect ofAphelenchoides besseyi on 27 cultivars of rice （23japonica and 4 indica） was assessed in the field for two seasons during 2010 and 2011. The vigorous pathogenic nematodes culturing on Botrytis cinerea were used for this experiment. Inoculation was carried out at the tilling stage; the growth parameters and nematode population were recorded at the end of growth of rice plants. The results showed that the cultivars differed in their response to infection. Most of cultivars were lack of the characteristic symptom of white tip, which was seen less frequently than the other two symptoms, namely small grains and erect panicles; moreover, the expression of symptoms was probably hereditary. The infection lowered the values of all the measured biological parameters, namely length of the stem and of the panicle, the number of filled grains per panicle, and 100-grain weight, in all the cultivars. The final nematode population indicated that the threshold of economic damage had also been exceeded in 10 cultivars, and none of them was immune. Three japonica cultivars proved most vulnerable whereas Tetep, an indica type, showed a level of resistance potentially useful in controlling A. besseyi.

关键词： Aphelenchoides besseyi rice symptom expression yield loss resistance assessment

Chemical mutagenesis and soybean mutants potential for identification of novel genes conferring resistance to soybean cyst nematode

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Journal of Integrative Agriculture 2018年第12期17卷 2734-2744页

作者： GE Feng-yong ZHENG Na ZHANG Liu-ping HUANG Wen-kun PENG De-liang LIU Shi-ming Institute of plant protection Chinese Academy of Agricultural Sciences College of plant protection Hunan Agricultural University

The resistance of soybean(Glycine max(L.) Merr.) to soybean cyst nematode(SCN, Heterodera glycines Ichinohe), which is a devastating pathogen in soybean production and causes a large quantity of annual yield loss worldwide, can shift during the long-term interaction and domestication. It is vital to identify more new resistance genetic sources for identification of novel genes underlying resistance to SCN for management of this pathogen. In the present study, first, two ethane methylsulfonate-mutagenesis soybean M2 populations of PI 437654, which shows a broad resistance to almost all of SCN races, and Zhonghuang 13, which is a soybean cultivar in China conferring strong resistance to lodging, were developed. Many types of morphological phenotypes such as four-and five-leaflet leaves were observed from these two soybean M2 populations. Second, 13 mutants were identified and confirmed to exhibit alteration of resistance to SCN race 4 through the forward genetic screening of 400 mutants of the PI 437654 M2 population, the rate of mutants with alteration of SCNinfection phenotype is 3.25%. Third, these identified mutants were further verified not to show any changes in the genomic sequences of the three known SCN-resistant genes, GmSHMT08, GmSNAP18 and GmSANP11, compared to the wildtype soybean; and all of them were still resistant to SCN race 3 similar to the wild-type soybean. Taken together, we can conclude that the 13 mutants identified in the present study carry the mutations of the new gene(s) which contribute(s) to the resistance to SCN race 4 in PI 437654 and can be potentially used as the genetic soybean sources to further identify the novel SCN-resistant gene(s).

关键词： soybean ethane methylsulfonate-mutagenesis populations mutants soybean cyst nematode race 4 resistance

Rice Grain Damage by Combination and Sequence Infestations by the Rice Leaffolder, Cnaphalocrocis medinalis Guenee(Lepidoptera: Pyralidae), and the White-Backed Rice planthopper, Sogatella furcifera Horváth(Hemiptera: Delphacidae)

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Journal of Integrative Agriculture 2014年第11期13卷 2460-2470页

作者： JIANG Li-ben ZHU Zhan-fei GE Lin-quan YANG Guo-qing WU Jin-cai School of plant protection Yangzhou University

The rice leaffolder （RLF）, Cnaphalocrocis medinalis Guenée （Lepidoptera：Pyralidae）, and the white-backed rice planthopper （WBPH）, Sogatella furcifera Horváth （Hemiptera： Delphacidae）, are major insect pests in China and several other Asian countries. These two pests commonly occur simultaneously or in a temporal sequence. Thus, the investigation of the effect of complex infestations or temporal sequence infestations by these pests on rice yield has a practical signiifcance for the control of these pests. The present study comprised experiments with the following four different variables in potted rice at the tillering stage：single pest species infestation, complex infestation, complete combination infestation and temporal sequence infestation （C. medinalis infestation prior to S. furcifera and S. furcifera infestation prior to C. medinalis）. The results showed that the four infestations resulted in a signiifcant decrease in 1 000-grain weight （1 000GW） and rate of yield loss （RYL） but an increase in blighted grain rate （BGR）, with a signiifcant positive correlation with the infestation density. However, the inlfuences of the complex infestation, complete combination infestation or sequence infestation on the 1 000GW, BGR and RYL were greater than those of the single pest species infestations but did not have addition effects, i.e., the effects of the complex infestation and combination infestation or sequence infestation on the 1 000GW, BGR and RYL were less than the additive effects of the two single pest species infestations at the same densities. In the condition of the same total infestation pressure, no signiifcant differences in the 1 000GW, BGR and RYL were found between C. medinalis infestation prior to S. furcifera and S. furcifera infestation prior to C. medinalis as well as between the sequence infestation and the complex infestation.

关键词： Cnaphalocrocis medinalis Sogatella furcifera complex infestation temporal sequence infestation rice yield loss

Use of chlorophyll fluorescence and P700 absorbance to rapidly detect glyphosate resistance in goosegrass(Eleusine indica)

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Journal of Integrative Agriculture 2015年第4期14卷 714-723页

作者： ZHANG Tai-jie FENG Li TIAN Xing-shan YANG Cai-hong GAO Jia-dong Institute of plant protection Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection

The rapid detection of glyphosate resistance in goosegrass（Eleusine indica） will enhance our ability to respond to new resistant populations of this major weed. Chlorophyll fluorescence（Fluo） and P700（reaction center chlorophyll of photosystem I） absorbance were analyzed in one biotype of goosegrass that is resistant to glyphosate and in another that remains sensitive to the herbicide. Both biotypes were treated with a foliar spray of glyphosate. Differences in photosystem II maximum quantum yield（Fv/Fm）, effective photochemical quantum yield（Y（II））, and non-photochemical quenching（NPQ） between the biotypes increased over time. Values for Fv/Fm and Y（II） differed between the two biotypes 24 h after treatment（HAT）. Differentiated activities and energy dissipation processes of photosystem II（PSII） and energy dissipation processes of photosystem I（PSI） were manifested in the two biotypes 24 HAT with 20 mmol L–1 glyphosate. Differentiated energy dissipation processes of PSI were still apparent 24 HAT with 200 mmol L–1 glyphosate. These results indicate that the Fluo parameters related to PSII activity and energy dissipation and the P700 parameters related to energy dissipation are suitable indicators that enable rapid detection of glyphosate resistance in goosegrass.

关键词： Eleusine indica glyphosate resistance chlorophyll fluorescence P700

Population Genetic Analysis of Blumeria graminis f. sp. tritici in Qinghai Province, China

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Journal of Integrative Agriculture 2014年第9期13卷 1952-1961页

作者： XU Zhi DUAN Xia-yu ZHOU Yi-lin GUO Qing-yun YAO Qiang CAO Shi-qin State Key Laboratory for the Biology of plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Institute of plant protection Qinghai Academy of Agricultural and Forestry Institute of plant protection Gansu Academy of Agricultural Sciences

To gain more precise information about molecular genetic variation for wild populations of Blumeria graminis f. sp. tritici from Qinghai Province, China, 38 single-colony isolates were purified from samples collected from Haidong District, Xining City and Hainan Tibetan Autonomous Prefecture in 2010. The virulence of 21 isolates among them was tested at seedling stage on 34 wheat cultivars（lines） carrying known powdery mildew（Pm） resistant genes. The results showed that V1 a, V3 a, V3 c, V3 e, V5 a, V6, V7, V8 and V19 had high virulence frequencies（〉75%）, indicating a wide distribution; and V1 c, V5 b, V12, V13, V16, V21, VXBD, V2＋6, V2＋Mld and V4＋8, with less distribution, appeared to be lower in frequencies（0-20%）. The Nei＇s gene diversity（H）, Shannon＇s information index（I） and the percentage of polymorphic loci（P） were 0.23, 0.35 and 67.65%, respectively, which revealed a virulent diversity. The results from single nucleotide polymorphisms（SNPs） of 38 isolates showed that three housekeeping genes were found to contain a total of 9 SNP sites. 10 haplotypes（H1-H10） were inferred from the concatenated sequences, with 1 haplotype（H1） comprising of over 55% of Qinghai population. Phylogenic analysis did not show obvious geographical subdivision between the isolates. A multilocus haplotype network presented a radial structure, with H1 in the central as an inferred ancestor. Using analysis of molecular variance（AMOVA）, we found 1.63% of the total variation was among populations and 98.37% within populations, with a low fixations index（FST=0.01634, P〈0.05）. This revealed a relatively high genetic diversity but a low genetic divergence in Qinghai population. Moreover, the molecular data on gene flow（Nm=6.32） confirmed the migration of pathogen populations among areas in Qinghai Province.

关键词： Blumeria graminis f.sp.tritici virulence single nucleotide polymorphisms haplotype

Investigating the mechanisms of glyphosate resistance in goosegrass(Eleusine indica) population from South China

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Journal of Integrative Agriculture 2015年第5期14卷 909-918页

作者： ZHANG Chun FENG Li HE Ting-ting YANG Cai-hong CHEN Guo-qi TIAN Xing-shan Guangdong Provincial Key Laboratory of High Technology for plant protection Institute of Plant ProtectionGuangdong Academy of Agricultural Sciences

Glyphosate has been used worldwide for nearly 40 years,and 30 types of resistant weeds have been *** is mass-produced and widely used in China,but few studies and reports on glyphosate-resistant weeds and resistance mechanisms *** studies found a goosegrass species with high glyphosate resistance from orchards in South China and its glyphosate resistant mechanism was described in this *** cDNAof 5-enolpyruvylshikimate-3-phosphate synthase（EPSPS,EC 2.***.1.19）,the target enzyme of glyphosate,was cloned from the glyphosate-resistant and-susceptible goosegrass,respectively,and referred as EPSPS-R and *** Pro106 residue was known to be involved in the glyphosate resistance in most goosegrass ***,sequence analysis did not find the mutation at the Pro106 residue in the R biotype EPSPS amino acid *** residue 133 and 382 was mutated in the R biotype EPSPS amino acid sequence instead,but it did not affect the EPSPS-S and EPSPS-R genes sensitivities to ***-PCR and Western blot analyses suggested that EPSPS mRNA and protein are mainly present in the shoot tissues both in the R and S goosegrass *** EPSPS-R rapidly responds to the glyphosate in R-biotype goosegrass and the induced expression was detected at 12 h post glyphosate *** mRNA and protein expression of EPSPS-R increased constantly as the increasing concentration of ***,the expression of the EPSPS-S was not induced significantly by glyphosate in the S goosegrass *** of real-time PCR results showed that the copy number of the EPSPS in R-biotype goosegrass was 4.7 times higher than that in the S goosegrass *** the results implied that EPSPS gene amplification might mainly caused the glyphosate resistance of a goosegrass population collected from orchards in South China.

关键词： populations shoot residue susceptible constantly translocation instead implied confer chlorophyll

Assessment of potential dermal and inhalation exposure of workers to the insecticide imidacloprid using whole-body dosimetry in China

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Journal of Environmental Sciences 2015年第1期27卷 139-146页

作者： Lidong Cao Bo Chen Li Zheng Dongwei Wang Feng Liu Qiliang Huang Institute of plant protection Chinese Academy of Agricultural Sciences College of plant protection Shandong Agricultural University School of Chemistry and Chemical Engineering Nanjing University

In China, although improvements to the pesticide registration process have been made in la thirty years, no occupational exposure data are required to obtain a commercial license for pesticide product. Consequently, notably little research has been conducted to establish a exposure assessment procedure in China. The present study monitored the potential derm operator exposure from knapsack electric sprayer wheat field application of imidacloprid Liaocheng City, Shandong Province and in Xinxiang City, Henan Province, China, usin whole-body dosimetry. The potential inhalation exposure was determined using a person air pump and XAD-2 sample tubes. The analytical method was developed and validate including such performance parameters as limits of detection and quantification, linear rang recovery and precision. The total potential dermal and inhalation exposures were 14.20, 16.815.39 and 20.78 mL/hr, respectively, for the four operators in Liaocheng and Xinxian corresponding to 0.02% to 0.03% of the applied volume of spray solution. In all trials, the low part（thigh, lower leg） of the body was the most contaminated, accounting for approximate76% to 88% of the total exposure. The inhalation exposure was less than 1% of the tot exposure. Such factors as the application pattern, crop type, spray equipment, operat experience and climatic conditions have been used to explain the exposure distribution ov the different parts of the body. As indicated by the calculated Margin of Exposure, the typic wheat treatment scenarios when a backpack sprayer was used are considered to be safe terms of imidacloprid exposure.

关键词： Potential dermal and inhalation exposure Insecticide imidacloprid Whole body dosimetry Margin of exposure China

Influence of Host Shift on Genetic Differentiation of the Oriental Fruit Fly,Bactrocera dorsalis

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Journal of Integrative Agriculture 2014年第12期13卷 2701-2708页

作者： WAN Xuan-wu LIU Ying-hong LUO Lin-ming FENG Chuan-hong WANG Sheng MA Li College of plant protection Southwest University/Chongqing Key Laboratory of Entomology and Pest Control Engineering Sichuan plant protection Station

Invasion of the oriental fruit lfy, Bactrocera dorsalis, into new niches containing different food sources （a process referred to as host shift）, may cause population genetic differentiation and sympatric speciation. To attempt to infer that experimentally, test populations were established by transferring a subset of the original populations, which had been grown on banana for many generations, onto navel orange, and then subculturing the navel orange population and banana population for at least 20 generations. Four pairs of SSR primers with high polymorphism on laboratory strains were used to detect population genetic differentiation. All six tested populations （the 5th, 10th and 15th generations of B. dorsalis fed on banana and navel orange, respectively） were found to have low genetic diversity. Furthermore, the genetic diversity of the navel orange populations was found to decline after being crossed for several generations. Populations initially were deviated from Hardy-Weinberg equilibrium, however, equilibrium was achieved with increasing numbers of generations in both of the host populations. Limited gene lfows were found among the six populations. The Nei’s standard genetic distances between the two host populations of the same generation were initially low, but increased with generation number. Genetic distances between banana and navel orange populations of the same generation were lower than genetic distances between different generations grown on the same host plant. Analysis of molecular distance （AMOVA） results based on generation groups and host groups demonstrated that genetic variation among generations was greater than that between the two host populations. The results indicated that population genetic differentiation occurred after the host shift, albeit at low level. Biogeography and taxonomy of the B. dorsalis complex revealed that speciation of B. dorsalis might be tightly associated with host shift or host specialization of B. dorsalis foll

关键词： Bactrocera dorsalis oriental fruit lfy host shift population genetic differentiation sympatric speciation

Selectivity and sublethal effects of some frequently-used biopesticides on the predator Cyrtorhinus lividipennis Reuter(Hemiptera: Miridae)

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Journal of Integrative Agriculture 2019年第1期18卷 124-133页

作者： DAI Wei LI Yao ZHU Jun GE Lin-quan YANG Guo-qing LIU Fang College of Horticulture and plant protection Yangzhou University

The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata *** this study,the selective toxicity of seven commercial biopesticides for *** was examined under laboratory conditions; abamectin was the most selective to ***,followed by matrine and ***,rotenone,Bacillus thuringiensis and Beauveria bassiana showed less selectivity for ***,matrine,abamectin and azadirachtin were selected for sublethal assessments with respect to *** due to their high toxicities to *** treated with sublethal concentrations(LC10 and LC20) of the three biopesticides could distinguish volatiles released from healthy and ***-infested plants indicating that the biopesticides tested did not affect the foraging ability of surviving *** decreased the consumption capability of *** when the densities of *** were 20,30,40 and 50 insects per *** concentration treatment did not impact the pre-oviposition period or egg hatchability of ***,the fecundity of *** exposed to azadirachtin and abamectin increased by 27–41% compared to the untreated *** summary,abamectin or matrine together with *** could be considered an effective,sustainable pest management strategy for rice.

关键词： biopesticides Cyrtorhinus lividipennis selective toxicity sublethal effects