检索结果-南通市图书馆

Isolation, Phenotypic Identification and Fingerprint by RAPD-PCR of Oxalotrophoic Burkholderia cepacia from Egyptian Fertile Soil

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Life Sciences 2012年第7期6卷 732-739页

作者： Hala Abou Shady Mona Kilany Abd EI Gawad Qadria Genedi Wafaa Farouk microbiology dept. Faculty of Science Ain Shams University Cairo 11566 Egypt microbiology dept. National Organization for Drug Control and Research (NODCAR) Cairo 12553 Egypt

Soil oxalate degrading bacteria is an important source for removing of toxic oxalate compounds. Isolation, screening, characterization, identification and fingerprint of the soil oxalate degrading bacteria from Egyptian fertile soil was aimed in this work. Isolated bacteria were first characterized using API 20 NE system. Nine RAPD primers were used in genetic fingerprinting of the characterized bacteria using RAPD-PCR. The preliminary characterization and polyphasic taxonomy for mesophilic, aerobic oxalate-degrading bacteria isolated from Egyptian fertile soil including auxanography using API galleries confirmed that the isolate belonged to the genus Burkholderia cepacia, the percentage of identity was 99.8% for 5167777 profiles. Fingerprinting by RAPD-PCR showed a DNA fingerprint ranging from 0 to 9 bands, over a size range of 344 bp to 3,280 bp.

关键词： Burkholderia cepacia oxalotrophic RAPD-PCR fingerprint API 20 NE isolation identification.

The Poly(vinyl alcohol)-Immobilized Photobacteria for Toxicology Monitoring

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering（科研） 2012年第10期4卷 118-120页

作者： Kristina A. Alenina Leyla E. Aleskerova Polina B. Kascheyeva Anvar D. Ismailov dept. of microbiology Biological Faculty Moscow State University Moscow Russia

The present work is undertaken to studies the important factors affecting the stability of a light emission at immobilized photobacteria, and application PVAGs luminous biosensors for biomonitoring of toxicants. The intensity and stability of a light emission is competently controlled by: 1) intensity and persistens of a luminescent cycle using bacterial strain;2) type of the carrier and the composition of the gel-formation medium;3) freeze-thawing procedures;4) physical and chemical conditions of storage and application.

关键词： Component Bioluminescence Photobacteria Immobilization Poly (Vinyl Alcohol)

Antimicrobial Activity of Five Yemeni Medicinal Plants against Selected Human Pathogenic Bacteria and Fungi

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2019年第10期10卷 1699-1707页

作者： Nagi Al-Haj Alariqi Reem Hassan Al-Shamahy Khaled Al-Moyed Saleh S. Bahaj Ammar Jaber dept. of Medical microbiology Faculty of Medicine and Health Sciences Sana’a University Sana’a Yemen

Background: The emergence and spread of antibiotic resistance, as well as the evolution of new strains of disease causing agents, are of great concern to the global health community. Nowadays, reports have indicated that many of healthcare-associated, antimicrobial-resistant bacteria are not only multidrug resistant pathogens but also broadly drug-resistant and pandrug-resistant bacteria. Objective: This study is focused on exploring the antimicrobial properties of five different plants that are commonly being used as traditional medicines in Yemen against Gram positive, Gram negative bacteria and fungi. Methods: The antimicrobial potential of five different plant extracts was screened against selected human pathogenic bacteria and fungi. Methanolic extracts of Dodonaea viscose, Plantago lanceolata, Withania samnifera, Pulicaria crispa, and Rumex nervosus were subjected to a test of their antimicrobial properties by Modified Agar Diffusion and Minimum Inhibitory Concentration. Results: The results indicated that all the five plants extract showed antibacterial activity against a selected microorganism at a concentration of 60 mg/ml. The highest potential was observed in the extract of W. somnifera against S. taphyloccocus aureus, Pseudomonas aeruginosa, Proteus miarbilis and Candida albicans with zone of inhibition of 23 mm, 20.7 mm, 20 mm and 25.3 mm, respectively. R. nervosus also showed the highest MIC against test organisms. Only two medicinal plant Pulicaria crispa, and Rumex nervosus extracts showed non antifungal activity. Conclusion: The experiment confirmed the efficacy of some selected plant extracts as natural antimicrobial and can be potential sources for the synthesis of antibacterial drugs.

关键词： Antimicrobial Activity Plant Extract Multidrug Resistant Yemen

Dissipation of chlorpyrifos, pendimethalin, and thiophanate-methyl pesticides in compost based-versus peat-moss based-biomixture of biobeds

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Waste Disposal and Sustainable Energy 2023年第4期5卷 461-470页

作者： Talaat N.M.El-Sebai Wafaa M.H.Zidan Alaa Marzouk Marion Divers Fabrice Martin-Laurent Agricultural microbiology dept.Agricultural and Biology Research Institute National Research CentreCairoEgypt Laboratory of Pesticide Analysis Agriculture Research CentreCairoEgypt Laboratory of Soil microbiology National Institute of Agricultural ResearchDijonFrance

Biobed is a smart bioremediation system used to treat point-source pesticide *** is the main component of biobeds,and pesticide dissipation is affected by its *** study aimed to compare the effectiveness of compost-based(C)versus peat-moss-based(P)biomixtures of biobeds on tested pesticide *** concentrations(25,50,and 75 mg/kg)of chlorpyrifos,pendimethalin,and thiophanate methyl were added separately and as a mix to both biomixtures C and *** data showed the effect of biomixture type on the dissipation of the tested *** chlorpyrifos,its dissipation rate in biomixture P was more rapid than that in biomixture *** result was confirmed by the mineralization kinetic experiment,since 25%of the initial ^(14)C-chlorpyrifos concentration accumulated in the form ^(14)CO_(2) in biomixture P compared to only 14%in biomixture *** addition,the chlorpyrifos dissipation rate was influenced by the initial concentration when applied individually in biomixture *** contrast,biomixture C was more effective at pendimethalin dissipation than biomixture P,since>76%of pendimethalin was dissipated in biomixture C versus 67%in biomixture P at the same incubation *** thiophanate-methyl,the abilities of both biomixtures C and P were similar and less efficient than those of the other tested *** addition of the three tested pesticides to biomixture C only had a positive effect on both chlorpyrifos and thiophanate-methyl dissipation,while pendimethalin dissipation was similar when applied separately or as a *** activity was stimulated by the addition of separately or mixed pesticides to biomixtures C and P as measured by dehydrogenase activity.

关键词： Biopurification system Pesticides Contamination Point-source Bioremediation Biomixture

STRUCTURAL STUDIES ON AGAR FRACTIONS EXTRACTED SEQUENTIALLY FROM CHINESE RED SEAWEEDS:GRACILARIA SJEOSTEDTII, G. TEXTORII AND G. SALICORNIA USING13C-NMR AND IR SPECTROSCOPY

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 1988年第2期6卷 87-103页

作者：纪明侯 M.Lahaye W.Yaphe Institute of Occanology Academia Sinica Qingdao dept.of microbiology & Immunology McGill University Montreal Canada CanadaM. Lahaye and W. Yaphe(dept.of microbiology & Immunology McGill University Montreal Canada)

The polysaccharides of three species of Chinese Gracilaria (Rhodophyta) were extracted by sequential solvent extraction and the chemical structures of the fractions obtained were investigated by using 13C-NMR and IR *** 13C-NMR spectra showed that the main extract, cold water fraction, from G. sjeostedtii consisted of repeating disaccharides of agarobiose and (1→3)-β-D-galactopyranosy 1-(1→4)-α-L-galactopyranose 6-sulfate, the latter being a precursor to agarobiose and converted to agarobiose after alkali modification. The component of the 60% ethanol fraction from G. textorii was composed mainly of agarose 6-sulfate, 6-O-methyl-agarose and some amount of agarose, whose structures remained unchanged after alkali treatment. The autoclave extract from G. salicornia revealed a complex structure composed of 6-0-methyl-agarose, 2-0-methyl-agarose, branched 4-0-methyl-L-galactose and agarose 4-sulfate, All the substiluents in these agaroses, were stable against alkali treatment. The alkali ex

关键词： NMR TEXTORII AND G C-NMR AND IR SPECTROSCOPY SALICORNIA USING STRUCTURAL STUDIES ON AGAR FRACTIONS EXTRACTED SEQUENTIALLY FROM CHINESE RED SEAWEEDS IR

Anti-inflammatory effects of bifidobacteria by inhibition of LPS-induced NF-κB activation

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第23期12卷 3729-3735页

作者： Christian U Riedel Francis Foata David Philippe Oskar Adolfsson Bernhard J Eikmanns Stephanie Blum Department of microbiology and Biotechnology University of Ulm89068 UlmGermany Immunology GroupNutrition and Health Dept.Nestlé Research CenterPO Box 44CH-1026 LausanneSwitzerland Immunology Group Nutrition and Health Dept.Nestlé Research CenterPO Box 44CH-1026 LausanneSwitzerland Department of microbiology and Biotechnology University of Ulm89068 UlmGermany

AIM： Different strains of bifidobacteria were analysed for their effects on HT-29 intestinal epithelial cells （IECs） in in vitro models both of the non-inflamed and inflamed intestinal epithelium. METHODS： A reporter gene system in HT-29 cells was used to measure levels of NF-KB activation after challenge with bifidobacteria or after bacterial pre-treatment following LPS challenge. IL-8 protein and pro-inflammatory gene expression was investigated using normal HT-29 cells. RESULTS： None of the bifidobacteria tested induced activation of nuclear factor κB （NF-κB） indicating that bifidobacteria themselves do not induce inflammatory events in IECs. However, six out of eight bifidobacteria tested inhibited lipopolysaccharide- （LPS-） induced NF-κB activation in a dose- and strain-dependent manner. In contrast, NF-κB activation in response to challenge with tumor necrosis factor-α （TNF-α） was affected by none of the tested bifidobacteria, indicating that the inhibitory effect of bifidobacteria is specific for LPS-induced inflammation in IECs. As shown with two of the six inhibitionpositive bifidobacteria, LPS-induced inhibition of NF-κB activation was accompanied by a dose-dependent decrease of interleukin 8 （IL-8） secretion and by lower mRNA levels for IL-8, TNF-α, cyclooxygenase 2 （Cox-2）, and intercellular adhesion molecule 1（ICAM-1）. CONCLUSION： Some strains of bifidobacteria are effective in inhibiting LPS-induced inflammation and thus might be appropriate candidates for probiotic intervention in chronic intestinal inflammation.

关键词： NF-κB Bifidobacteria Anti-inflammatory LPS

A five years study of fascioliasis and dicrocoeliasis in Iran's slaughterhouses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2008年第4期1卷 9-13页

作者： H.R.Tavakoli A.Mahmoodzadeh M.Hajia Nutrition & Food Hygiene dept.and Health Research Center Baqyatallah Medical Sciences UniversityTehranIran microbiology dept.and Research Center of Molecular Biology Baqyatallah.Medical Sciences UniversityTehranIran microbiology Dept.Research Center of Health Reference Laboratories of Iran

Objective:Fascioliasis and dicrocoeliasis are the two endemic parasitic diseases of Iran that make lots of economical damages *** study has been performed to evaluate infection rate of domesticated animals to fascioliasis and dicrocoeliasis among of slaughtered animals in slaughterhouses of ***:In this crosssectional study infection rate of slaughtered animals has been analyzed in 28 provinces of Iran during 5 years period(2003-2007).Results:It is indicated that infection rate was 4.84%to fascioliasis and dicrocoeliasis in this five-year study *** removed livers were 815 807 and 771 026 numbers from consumption cycle for fascioliasis and dicrocoeliasis respectively from 32 714 926 slaughtered *** highest infection rate was Gilan(20.91%),Mazandaran(16.36%),and Esfahan(9.95%) respectively among studied provinces while the lowest infection rate was indicated in Ilam(0.76%),Boushehr(0.84%),and Yazd(1.51%) respectively. Our data proved the highest frequency rate were identified in summer and autumn for both fascioliasis and ***:Fascioliasis and dicrocoeliasis are continuously increasing in slaughter animals of *** the results of current study can improve and move up activities against these zoonosis diseases.

关键词： Fascioliasis Dicrocoeliasis Frequency rate Slaughter animals Iran

Determination of Organochlorine Pesticide Residues in Pumpkin, Spinach and Sorrel Leaves Grown in Akwanga, Nasarawa State, Nigeria

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2018年第5期9卷 508-515页

作者： E. G. Ibrahim N. Yakubu L. Nnamonu J. M. Yakubu dept. of Pure and Applied Chemistry Plateau State University Bokkos Plateau Stae Nigeria Demonstration Secondary School College of Education Akwanga Nasarawa State Nigeria dept. of Chemistry and Agrochemical Technology University of Agriculture Ma-kurdi Benue State Nigeria dept. of microbiology Federal University Wukari Wukari Nigeria

Three green leafy vegetable samples of pumpkin leaves, spinach leaves, and sorrel leaves were collected from a farm in Akwanga and were tested for the presence of residues of organochlorine pesticides. The concentrations of all the pesticide residues in the vegetable samples were determined using GC/MS. Among the organochlorine pesticide p,p’-DDT was detected in pumpkin (0.75 mg/kg), spinach (0.319 mg/kg) and sorrel (0.219 mg/kg). θ-BHC and γ-BHC were detected only in pumpkin leaves (0.359 mg/kg and 0.647 mg/kg respectively). Dieldrin was detected in spinach and sorrel (0.124 mg/kg and 0.053 mg/kg respectively). Endrin was detected in pumpkin (0.732 mg/kg) and Aldrin in sorrel (0.095 mg/kg). All these values were above the maximum residue limit (MRL) value of the pesticides. Endosulfan II was detected in sorrel (0.306 mg/kg) below the MRL. The levels of most of the pesticide residues found in vegetables were above the maximum residue limits (MRLs) that call for laws to regulate the use and circulation of such chemicals. Routine monitoring of pesticide residues in this study area is necessary for the prevention, control and reduction of environmental pollution, to minimize health risks.

关键词： Akwanga Organochlorine Pesticide Residues Maximum Residue Limit Vegetables

Prevalence of Organophosphorous Pesticide Residues in Pumpkin, Spinach and Sorrel Leaves Grown in Akwanga, Nasarawa State, Nigeria

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Protection 2018年第5期9卷 516-524页

作者： E. G. Ibrahim N. Yakubu L. Nnamonu J. M. Yakubu dept. of Pure and Applied Chemistry Plateau State University Bokkos Jos Plateau Stae Nigeria Demonstration Secondary School College of Education Akwanga Nasarawa State Nigeria dept. of Chemistry and Agrochemical Technology University of Agriculture Ma-kurdi Benue State Nigeria dept. of microbiology Federal University Wukari Wukari Nigeria

Three green leafy vegetable samples of pumpkin leaves, spinach leaves, and sorrel leaves were collected from three different locations in a farm in Akwanga and were tested for the presence of organophosphorus (OP) compounds. The concentrations of all the pesticide residues in the vegetable samples were determined using gas chromatography with mass spectrometry (GC-MS). The organophosphorus pesticides detected include carbaryl (0.052 mg/kg) in pumpkin, this is below European maximum residues limit (EU MRL) of 0.1 mg/kg, Dimethoate was found in pumpkin (0.165 mg/kg), spinach (0.103 mg/kg) and sorrel (0.250 mg/kg) all above the EU MRL of 0.05 mg/kg. Dichlofenthion was detected in pumpkin (0.308 mg/kg), pirimiphos methyl was detected in pumpkin and spinach (0.428 mg/kg and 0.149 mg/kg respectively), all these were below the EU MRL of 0.5 mg/kg. Chlorpyrifos was found in spinach (0.230 mg/kg) and sorrel (0.192 mg/kg) only spinach was above the EU MRL of 0.2 mg/kg. The pesticide residue Bromophosethyl was detected in all vegetables, pumpkin leaves (0.501 mg/kg), sorrel (1.571 mg/kg) and spinach (7.981 mg/kg) all above the EU MRL of 0.5 mg/kg. The remaining pesticides detected were all below their EU MRL value;these are Ethion found in spinach (0.167 mg/kg), Methyl Parathion in spinach (0.103 mg/kg) and sorrel (0.335 mg/kg). The levels of some of the organophosphorus pesticide residues found in vegetables were above the maximum residue limits (MRLs) set by the European Union. This calls for laws to regulate the use and circulation of such chemicals. Based on the observation made in these studies, it is proposed that more extensive monitoring investigation covering all vegetables part in Akwanga Nasarawa state be carried out to find the exact position of pesticide residues.

关键词： Akwanga Pesticide Residues Organophosphorous Maximum Residue Limit