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food Science and Human Wellness 2024年第2期13卷 765-777页

作者： Qian Chen Chunxia Mei Min Guo Botao Wang Haiqin Chen Jianxin Zhao Gang Wang Wei Chen State Key Laboratory of food Science and Resources Jiangnan UniversityWuxi 214122China School of food Science and Technology Jiangnan UniversityWuxi 214122China National engineering Research Center for Functional food Jiangnan UniversityWuxi 214122China (Yangzhou)Institute of food Biotechnology Jiangnan UniversityYangzhou 225004China Bloomage Biotechnology Co. Ltd.Jinan 250000China

Lipid metabolism disorders commonly occur during *** deficiency has been shown to lead to excessive energy intake and abnormal lipid metabolism in ovariectomized rats,resulting in *** exhibit anti-obesity properties,and their underlying mechanism has been widely *** this study,we demonstrated the metabolic benefits of Lactobacillus plantarum CCFM1180 in suppressing appetite,controlling body weight,correcting obesity-induced abnormalities,enhancing liver lipid metabolism,and protecting liver function in estrogen-deficient *** mechanisms associated with the anti-obesity and anti-dyslipidemia effects of CCFM1180 on estrogen-deficient rats were *** results showed that CCFM1180 dramatically reduced food intake by activating the expression of estrogen receptor alpha(ERα)and increasing the level of leptin in abdominal adipose *** changes,combined with the increased butyrate concentration and recovered bile acid structure,helped enhance lipid ***,CCFM1180 treatment was found to be safer than exogenous estrogen ***,*** CCFM1180 could be considered a new therapeutic strategy for preventing and alleviating menopausal lipid abnormalities.

关键词： Bile acid Gut microbiota Menopause Ovariectomy Short chain fatty acid

Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum

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Synthetic and Systems Biotechnology 2024年第1期9卷 99-107页

作者： Chunjian Li Pengdong Sun Guoqing Wei Yuqi Zhu Jingyuan Li Yanfeng Liu Jian Chen Yang Deng College of food Science and engineering Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Special food Processing(Co-construction by Ministry and Province) Ministry of Agriculture Rural AffairsQingdao Agricultural UniversityQingdao 266109China Shandong Technology Innovation Center of Special food Qingdao 266109China Qingdao Special food Research Institute Qingdao 266109China Qingdao Nuoan Baite Biotechnology Co. Ltd.Qingdao 266109China Science Center for Future foods Engineering Research Center of Ministry of Education on Food Synthetic Biotechnologyand Jiangsu Province Engineering Research Center of Food Synthetic BiotechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China

Creatine is a naturally occurring derivative of an amino acid commonly utilized in functional foods and ***,the current industrial synthesis of creatine relies on chemical processes,which may hinder its utilization in certain ***,a biological approach was devised that employs whole-cell biocatalysis in the bacterium Corynebacterium glutamicum,which is considered safe for use in food production,to produce safe-for-consumption *** objective of this study was to identify a guanidinoacetate N-methyltransferase(GAMT)with superior catalytic activity for creatine *** employing whole-cell biocatalysis,a gamt gene from Mus caroli(Mcgamt)was cloned and expressed in *** ATCC 13032,resulting in a creatine titer of 3.37 g/***,the study employed a promoter screening strategy that utilized nine native strong promoters in *** to enhance the expression level of *** highest titer was achieved using the P1676 promoter,reaching 4.14 g/*** conditions of whole-cell biocatalysis were further optimized,resulting in a creatine titer of 5.42 g/*** is the first report of successful secretory creatine expression in ***,which provides a safer and eco-friendly approach for the industrial production of creatine.

关键词： Creatine Corynebacterium glutamicum Whole-cell biocatalysis Guanidinoacetate N-Methyltransferase food additive

Changes in physicochemical characteristics of wheat flour and quality of fresh wet noodles induced by microwave treatment

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Grain & Oil Science and Technology 2024年第3期7卷 177-185页

作者： Jian Zhang Xuejie Li Xiujuan Ren Yanxia An Xiaoyan Song Yang Zhao Yaqing Wen Weifeng Zhang College of food Science and Technology Henan Agricultural UniversityZhengzhou 450000Henan ProvinceChina School of food Science and engineering Zhengzhou University of Science and TechnologyZhengzhou 450064Henan ProvinceChina

Fresh wet noodles(FWN) are popular staple foods due to its unique chewy texture and favorable taste. However,the development of FWN is limited by its short shelf life and high browning rate. It has been found that the quantity of original microorganisms in wheat flour produced by traditional method is relatively high, which is detrimental to the processing quality and storage stability of FWN. Consequently, it becomes imperative to decrease microorganisms in wheat flour. Microwave treatment has been regarded as a promising method in the food industry due to its potential in inhibiting microbial growth and inactivating enzymes without causing adverse effect on the food quality. This study aims to investigate the effects of microwave treatment of wheat kernels under different powers(1, 2, 3, 4, 5 kW) on the physicochemical properties of wheat flour and the quality of FWN. The results revealed that microwave treatment had a significant effect on microbial inhibition and enzyme inactivation, wherein the total plate count(TPC) and yeast and mold counts(YMC) decreased by 0.87 lg(CFU/g) and 1.13 lg(CFU/g) respectively, and PPO activity decreased from 11.40 U to 6.31 U. The dough quality properties, such as stability, extensibility, and starch viscosity, improved significantly under different microwave conditions. Confocal laser scanning microscopy(CLSM) images indicated that starch and proteins aggregated gradually in treated flour, altering rheological properties of dough. From the results of scanning electron microscopy(SEM), microwave treatment led to the appearance of disrupted structure in the gluten proteins, but the secondary structure of proteins altered slightly. Rheological properties of dough confirmed that the microwave treatment greatly affected processing characteristics of wheat flour products, with significant advantageous consequences on product quality, especially for textural properties of FWN. Furthermore, FWN darkening could be inhibited noticeably aft

关键词： Microwave treatment Sterilization Confocal laser scanning microscopy Rheological properties Protein structures

Hydrogel-supported poly(L-lactic acid) and polystyrene microsphere-based three-dimensional culture systems for in vitro cell expansion

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Frontiers of Materials Science 2024年第2期18卷 129-137页

作者： Huaying Hao Lihong Sun Jiaxuan Chen Jun Liang State Key Laboratory of food Nutrition and Safety Tianjin University of Science&TechnologyTianjin 300457China College of Light Industry Science and engineering Tianjin University of Science&TechnologyTianjin 300457China College of food Science and engineering Tianjin University of Science&TechnologyTianjin 300457China

The in vitro expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ *** objective of this paper was to achieve static cell culture in vitro through peptide hydrogel-supported microspheres(MSs).The peptides,with their gel-forming properties,microstructures,and mechanical strengths characterized,were found to have good support for the MSs and to be *** internal structures of poly(L-lactic acid)microspheres(PLLA-MSs)and polystyrene microspheres(PS-MSs)made in thelaboratory were observed and statistically analyzed in terms of particle size and pore size,following which the co-cultured MSs with cells were found to have good cell *** addition,three-dimensional(3D)culturing of cells was performed on the peptide and microcarrier composite scaffolds to measure cell viability and cell *** results showed that the peptides could be stimulated by the culture medium to self-assembly form a 3D fiber network *** the peptide-Ms composite scaffold-based cell culture system,further enhancement of the cell culture effect was *** peptide-Ms composite scaffolds have great potential for the application in 3D cell culture and in vitro cellexpansion.

关键词： microcarrier microsphere peptide hydrogel cell scaffold three-dimensional culture cell expansion

food-derived protein hydrolysates and peptides:anxiolytic and antidepressant activities,characteristics,and mechanisms

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food Science and Human Wellness 2024年第3期13卷 1168-1185页

作者： Wenhui Li Yu Xi Junru Wang Yinxiao Zhang He Li Xinqi Liu Beijing Advanced Innovation Center for food Nutrition and Human Health Beijing Engineering and Technology Research Center of Food AdditivesNational Soybean Processing Industry Technology Innovation CenterBeijing Technology and Business UniversityBeijing 100048China

Globally,the prevalence of anxiety and depression has reached epidemic ***-derived protein hydrolysates and peptides delivered through dietary supplementation can avoid the negative risks associated with traditional pharmaceuticals while delivering superior anxiolytic and antidepressant *** review summarizes current research on food-derived anxiolytic and antidepressant protein hydrolysates and peptides,and subsequently analyses their physicochemical characteristics and elaborates on their *** aim of this work is to contribute to the in-dept. study and provide a theoretical foundation for the development of related products to better serve patients with anxiety and depression.

关键词： Anxiolytic Antidepressant Peptides Protein hydrolysates Neurotransmitter

Simulation of rice grain breakage process based on Tavares UFRJ model

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Particuology 2024年第10期93卷 65-74页

作者： Shaohang Shen Shouyu Ji Dan Zhao Yanlong Han Hao Li Ze Sun Zhuozhuang Li Anqi Li Wenyu Feng Jiaming Fei Fuguo Jia Yang Li College of engineering Northeast Agricultural UniversityHarbin150030China School of Water Conservancy and Civil engineering Northeast Agricultural UniversityHarbin150030China College of food Science Northeast Agricultural UniversityHarbin150030China

Understanding the breakage characteristics of rice grains is an important means to reduce rice breakage rate. However, the dynamic breakage mechanism of rice grain is unclear due to the lack of a reasonable breakage model. In this study, the uniaxial compression test and drop weight test of single rice were carried out, the breakage model of rice grain was constructed, the reliability of rice model was verified by the experiment and simulation results. The results showed that the fracture energy distribution of rice can be obtained by uniaxial compression test, the specific fracture energy of rice accords with a lognormal distribution, and the median specific fracture energy of rice is 479.75 J/kg. The damage accumulation coefficient and fragment size distribution of rice can be acquired by drop test, the result of damage accumulation coefficient of rice was 4.3. Rice grain breakage mainly occurs in the milling section of the vertical circulation rice mill.

关键词： Rice breakage mechanism Discrete element method Parameter calibration

Lactobacillus plantarum 104 Cell-freesupernatant improved bile acid metabolism by regulating gut microbiota of high-fat diet mice

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food Science and Human Wellness 2024年

作者： Xia Li Shanshan Wei Ruixue Zhao Yue Zhang Zixin Gao Zhanpeng Wang Yu Wang Bo Nan Yuhua Wang College of food Science and engineering and Jilin Province Innovation Center for food Biological Manufacture， Jilin Agricultural University

Lactiplantibacillusplantarum 104， which was previously screened in the laboratory， has been confirmed to have the effect of lowering lipids and regulating intestinal microbial homeostasis. However， the mechanism of action of its fermentation supernatant in alleviating metabolic disorders is ***104 cell-freesupernatant (LP104s) was fed to high-fat C57BL/6N mice for 8 weeks. The intervention of LP104s increased the concentration of conjugated bile acids in the ileum， especially taur-α/β-muricholic acid sodium salt (T-α-MCA or T-β-MCA)， inhibited intestinal farnesoid X receptor (FXR)-related signaling pathways. LP104s reduced cholesterol levels by increasing the synthesis of hepatic cholic acid (CA)， chenodeoxycholic acid (CDCA)， and the excretion of bile acids in feces. Moreover， western blotting results showed that TLR2/NF-?B signaling pathway expression was inhibited. In addition， 16S rDNA sequencing results showed that LP104s regulated the relative abundance of bacteria associated with inflammatory response and obesity (Prevotella， Ruminococcus， and Clostridium). Therefore， this paper further elaborates the role of gut microbiology and bile acids in improving metabolism from the perspective of secondary bile acids and intestinal chronic inflammation and also lays a theoretical foundation for the next step in the development of fermentation products of this strain.

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Biocatalytic enhancement of laccase immobilized on ZnFe_(2)O_(4) nanoparticles and its application for degradation of textile dyes

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Chinese Journal of Chemical engineering 2024年第4期68卷 216-223页

作者： Yuhang Wei Qingpeng Zhu Weiwei Xie Xinyue Wang Song Li Zhiming Chen School of Chemical and Environmental engineering Anhui Laboratory of Functional Coordinated Complexes for Materials Chemistry and ApplicationAnhui Polytechnic UniversityWuhu 241000China School of Biology and food engineering Anhui Polytechnic UniversityWuhu 241000China

Efficient and convenient treatment of industrial dyeing wastewater is of great significance to guarantee human and animal *** work presented the enhanced catalytic activity at pH 3.0 of laccase immobilized on amino-functionalized ZnFe_(2)O_(4) nanoparticles(ZnFe_(2)O_(4)-laccase)and its application for the degradation of textile *** to the existence of a large number of oxygen vacancies on the surface of the ZnFe_(2)O_(4) nanoparticles,negative ions accumulated on the magnetic carriers,which resulted in a harsh optimal pH value of the ZnFe_(2)O_(4)-*** activity assays revealed that the ZnFe_(2)O_(4)-laccase possessed superior pH and thermal stabilities,excellent reusability,and noticeable organic solvent ***,the ZnFe_(2)O_(4) laccase presented efficient and sustainable degradation of high concentrations of textile *** initial decoloration efficiencies of malachite green(MG),brilliant green(BG),azophloxine,crystal violet(CV),reactive blue 19(RB19),and procion red MX-5B were approximately 99.1%,95.0%,93.3%,87.4%,86.1%,and 85.3%,*** 10 consecutive reuses,the degradation rates of the textile dyes still maintained about 98.2%,92.5%,83.2%,81.5%,79.8%and 65.9%,*** excellent dye degradation properties indicate that the ZnFe_(2)O_(4)-laccase has a technical application in high concentrations of dyestuff treatment.

关键词： ZnFe_(2)O_(4)-laccase Catalytic activity Stability and reusability Degradation of textile dye

Smart Colorimetric Corn Starch Films Combined with Anthocyanin-Loaded Glutenin-Carboxymethyl Chitosan Nanocomplexes for Freshness Monitoring of Chilled Pork

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Journal of Renewable Materials 2024年第1期12卷 71-87页

作者： Juan Yan Wenchao Li Xianfang Zhang Shisheng Liu College of food Science and engineering Hainan UniversityHaikou570100China engineering Research Center for Utilization of Tropical Polysaccharide Resources Ministry of EducationHaikou570100China

In this study,intelligent,pH-responsive colorimetric films were prepared by encapsulating anthocyanins in nanocomplexes prepared from glutenin and carboxymethyl *** nanocomplexes were added to a corn starch matrix and used in the freshness monitoring of chilled *** effects of anthocyanin-loaded nanocomplexes on the physical,structural,and functional characteristics of the films were *** addition of anthocyanin-loaded nanocomplexes increased the tensile strength,elongation at break,hydrophobicity,and light transmittance of the films while decreasing their water vapor *** is because new hydrogen bonds are formed between the film components,resulting in a more homogeneous and dense *** colorimetric film has a significant color response to pH *** films were used in experiments on the freshness of chilled pork,in which the pH changes with changing freshness *** results show that the colorimetric film can monitor changes in the freshness of chilled pork in real time,where orange,pink,and green represent the fresh,secondary fresh,and putrefied states of pork,***,the intelligent colorimetric film developed in this study has good application potential in the food industry.

关键词： Carboxymethyl chitosan glutenin nanocomplexes anthocyanins pH indicator film chilled pork

Defect-induced synthesis of nanoscale hierarchically porous metal-organic frameworks with tunable porosity for enhanced volatile organic compound adsorption

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Nano Materials Science 2024年第4期6卷 467-474页

作者： Kuan Liang Weibiao Guo Linmei Li Huidong Cai Haiqi Zhang Jingjing Li Feng Xu Jian Yan Daofei Lv Hongxia Xi Chongxiong Duan Guangdong Provincial Key Laboratory of Intelligent food Manufacturing Foshan University School of Materials Science and Hydrogen engineering Foshan University School of Chemistry and Chemical engineering South China University of Technology School of Environmental and Chemical engineering Foshan University

Nanoscale hierarchically porous metal-organic frameworks （NH-MOFs） synergistically combine the advantages of nanoscale MOFs and hierarchically porous MOFs,resulting in remarkable characteristics such as increased specific surface area,greater porosity,and enhanced exposure of active ***,nanoscale hierarchically porous UIO-66 （UIO-66＿X） was synthesized using a defect-induced strategy that employed ethylene diamine tetraacetic acid （EDTA） as a *** introduced EDTA occupies the coordination sites of organic ligands,promoting the formation and growth of UIO-66 crystal nuclei and inducing defects during *** as-synthesized UIO-66＿X crystals exhibit a uniform distribution with an average size of approximately 100 *** addition,the total pore volume attains a remarkable value of 0.95 cm3g-1,with mesopores constituting 36.8%of the ***,the porosities of UIO-66＿X can be easily tuned by controlling the molar ratio of EDTA/Zr4+.In addition,the as-synthesized UIO-66＿X exhibits excellent adsorption capacities for n-hexane (344 mg g-1) and pxylene (218 mg g-1),which are 44.5%and 27.5%higher than those of conventional UIO-66,***,the adsorption behavior of n-hexane and p-xylene molecules in UIO-66＿X was investigated using density functional theory simulations.

关键词： Nanoscale hierarchically porous MOFs Tunable porosities Enhanced VOC adsorption