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Discrimination between Mature Vinegars of Different Geographical Origins by NIRS

河南省化学会2018年学术年会

作者：冯丹丹邓欣韬秦小转谢光辉李靖靖 School of Chemical engineering and food Science Zhengzhou Institute of Technology College of Chemistry and Chemical engineering Southwest University

Constructing all-carbon quaternary stereocenters promoted by Maruoka phase-transfer catalyst is one of the research topics in the field of asymmetric catalysis[1-3]. In this work,hollow organosilica/phase-transfer catalysis nano-microreactor was synthesized to circumvent the difficultytorecover expensive chiral catalysts and the limitation of mass transfer in heterogeneous ***, using polystyrene as hard template, core-shell microsphere PS/PVP@B&M was synthesized by co-conden-sation polymerization. The hollow organosilica microsphere B&M was obtained by etching template by chemical etching. Thenhollow organosilica/phase-transfer catalysisnano-microreactor, denoted as B&M@ PTC, wasachieved through click reaction of Maruokacatalyst with hollow organosilica microsphere B&M. The nano-microreactor B&M@PTChas nanospherical particles with ethyl-bridged organosilica in the shell which benefit for the mass transfer effect in heterogeneous catalysis. In themodel asymmetric α-alkylation reaction, the α-alkylation products with good enantioselectivities(86-91% ee) were obtained in high 85-96% yields. This work provided an strategy tosolvingthe recovery problem of expensive chiral Maruoka catalyst andconstructing high-performanceheterogeneous catalytic system.

关键词： hollow organosilica recycle catalysis Maruokaphase-transfer catalysis nano-microreactor

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Discrimination between Mature Vinegars of Different Geograph...

Structural Characteristics and Mechanical Properties of Rock Mass in the Field of Tianwan Nuclear Power Plant, China

第四届国际计算机与计算技术在农业中的应用研讨会

作者： Huishan Lu Zhengguang An Huanyu Jiang Yibin Ying College of Mechanical engineering & Automatization North University of China College of Biosystems engineering and food Science Zhejiang University

The feasibility of near infrared spectroscopy（NIRS） for discrimination between mature vinegar of different geographical origins（Taiyuan and Qingxu,China） is presented in this *** spectra were collected in transmission mode in the wavelength range of 800-2500 *** analysis models were developed based on Principal Component Analysis（PCA） and Discriminant Analysis（DA）.The prediction performance of calibration models in different wavelength range was also *** best models gave a96.3%classification of mature vinegars of the two geographical origins in the range of 800-2500 *** results demonstrated that NIRS techmque could be used as a rapid method for classification of geographical origin of mature vinegars.

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Journal of Shanghai Jiaotong university(Science) 2006年第4期11卷 512-517,524页

作者：周念清唐益群邓永华赵在立 dept. of Geotechnical Eng. Tongji Univ. The General Company of East China Survey Foundation Eng. of Metallurgical Industry Ministry Hebei Zhonghe Geotechnical Eng. Co. Ltd.

The structural characteristics and mechanical properties of the rock mass are important parts of the feasibility study on the nuclear power engineering field. In this study, by means of in situ investigation and statistics, the structural plane and joint fissure features of the rock mass were analyzed and discussed at different plots and different dept. scopes in the Tianwan Nuclear Power engineering field, the rock mass integrality and its weathered degree were evaluated respectively, and especially, the unfavorable geological phenomena of strongly-weathered cystid existing in the field were studied. According to the results of indoor rock mechanical tests, in combination with drilling, the shallow seismic prospecting, sonic logging and point load tests, the statistical results of physical and mechanical indices of rocks at key plots of the field were analyzed, and the design parameters of the field were calculated. It provided scientific basis for the foundation design of the nuclear power plant.

关键词： Tianwan Nuclear Power Plant structural plane joint fissure weathered degree rock mass integrality mechanical property

Identification of two novel nodule-specific genes from Astragalus sinicus L. by suppressive subtractive hybridization

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Chinese Science Bulletin 2007年第20期52卷 2797-2804页

作者： CHOU MinXia WEI XinYuan CHEN DaSong ZHOU JunChu College of Life Sciences Northwest Sci-Tech University of Agriculture and Forestry Yangling 712100 China College of food Science and engineering Northwest Sci-Tech University of Agricuiture and ForestryYangling 712100 China State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan 430070 China

To identify the genes involved in nodule formation and to increase usable molecular probes, a cDNA library of Astragalus sinicus genes specifically expressed in infected roots by Mesorhizobium huakuii 7653R is generated using a PCR-based suppressive subtractive hybridization (SSH) technique with two mRNA populations of infected and uninfected control roots. Two nodule-specific genes, AsIIC259 and AsG2511, are identified from infected roots of A. sinicus. The amino acid sequences deduced from the open reading frames (ORFs) reveal that AsIIC259 and AsG2511 encodes a polypeptide with 134 and 58 amino acids, respectively. A signal peptide sequence is predicted with high probability at the N-termini of the AsIIC259 and AsG2511. The motif searches show that the deduced polypeptide of AsIIC259 con- tains two N-glycosylation sites, a cAMP- and cGMP-dependent protein kinase phosphorylation site and a casein kinase II phosphorylation site. BLASTP searches reveal that AsIIC259 putative protein displays a low degree of similarity to a unique nodulin from Lupinus luteus nodules. No significant identity is displayed over the predicted polypeptides of AsG2511 with any published sequences. Virtual Northern blot and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses indicate that the two genes are expressed exclusively in inoculated roots and that their expression is 2―4 d later than that of the leghaemoglobin (Lb) gene during nodule development.

关键词：根瘤素共生关系杂交紫云英遗传细胞

Modeling Monthly Spatial Distribution of Ommastrephes bartramii CPUE in the Northwest Pacific and Its Spatially Nonstationary Relationships with the Marine Environment

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Journal of Ocean University of China 2018年第3期17卷 647-658页

作者： FENG Yongjiu LIU Yang CHEN Xinjun College of Marine Sciences Shanghai Ocean University Function Laboratory for Marine Fisheries Science and food Production Processes Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources Shanghai Ocean University National Distant-Water Fisheries engineering Research Center Shanghai Ocean University

There are substantial spatial variations in the relationships between catch-per-unit-effort(CPUE) and oceanographic conditions with respect to pelagic species. This study examines the monthly spatiotemporal distribution of CPUE of the neon flying squid, Ommastrephes bartramii, in the Northwest Pacific from July to November during 2004–2013, and analyzes the relationships with oceanographic conditions using a generalized additive model(GAM) and geographically weighted regression(GWR) model. The results show that most of the squids were harvested in waters with sea surface temperature(SST) between 7.6 and 24.6℃, chlorophyll-a(Chl-a) concentration below 1.0 mgm^(-3), sea surface salinity(SSS) between 32.7 and 34.6, and sea surface height(SSH) between-12.8 and 28.4 cm. The monthly spatial distribution patterns of O. bartramii predicted using GAM and GWR models are similar to observed patterns for all months. There are notable variations in the local coefficients of GWR, indicating the presence of spatial non-stationarity in the relationship between O. bartramii CPUE and oceanographic conditions. The statistical results show that there were nearly equal positive and negative coefficients for Chl-a, more positive than negative coefficients for SST, and more negative than positive coefficients for SSS and SSH. The overall accuracies of the hot spots predicted by GWR exceed 60%(except for October), indicating a good performance of this model and its improvement over GAM. Our study provides a better understanding of the ecological dynamics of O. bartramii CPUE and makes it possible to use GWR to study the spatially nonstationary characteristics of other pelagic species.

关键词： Ommastrephes bartramii catch-per-unit-effort (CPUE) oceanographic conditions geographically weighted regression (GWR) spatial nonstationary Northwest Pacific

Regulating Effect of Allicin(Diallyldisulfid-S-Oxide) on Respiration Function of Mitochondria from Beef Heart

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Chemical Research in Chinese Universities 2010年第5期26卷 785-791页

作者： SU Yan-bin TONG Li-fang LIU Pai LU Ming CHENG Ying-kun ZHANG Feng-rong SU Yan-wen DONG Jia-ning LI Zheng-qiang Key Laboratory for Molecular Enzymology ＆ engineering Ministry of Education Jilin University Changchun 130021 P R. China School of Chemical and Pharmaceutical engineering Jilin Institute of Chemical Technology Jilin 132022 P R. China Department of Infective Disease Therapeutics of China-Japan Union Hospital Jilin University Changchun 130033 P. R. China Jilin Provincial Institute for Drug and food Control Changchun 130033 P. R. China

To probe into regulating functions of allicin, diallyldisulfid-S-oxide, on electron transport in the inner membrane of mitochondria isolated from beef heart, under the anaerobic, hypoxia, and oxygenating conditions in the presence of rotenone（complex I inhibitor） at 37 ℃, we optimized the isolation method for isolating mitochondria from beef heart and used the technique for measuring dissolved oxygen content at the right moment so as to investigate the effects of allicin on the respiratory control of mitochondria in state 4 and state 3, P/O ratio, and respiratory control ratio（RCR）. The results show that the optimized isolated requirements were that all the operations were always kept at 0-4 ℃, the ratio between sample and isolation medium was 1：4, the filter was a four-layer gauze, two centrifugations in different sections. First section： 2500g, 10 min; second section 7000g, 20 min. The results indicate that the capacity of dissolved oxygen in state 3 was increased and that of dissolved oxygen in state 4 was almost stabilized in mitochondria from beef heart.

关键词： Allicin Respiration Mitochondrial Beef heart

Keemun black tea stems and leaves: Comparison of chemical characterization and anti-inflammatory activities

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food Science and Human Wellness 2024年

作者： Mengting Zhu Xiaoqing Rong Mingchun Wen Piaopiao Long Zongde Jiang Wen Xu Daniel Granato Liang Zhang State Key Laboratory of Tea Plant Biology and Utilization， Anhui Agricultural University Key Laboratory of Quality Evaluation of Chinese Medicine of the Guangdong Provincial Medical Products Administration， the Second Clinical College， Guangzhou University of Chinese Medicine International Joint Laboratory on Tea Chemistry and Health Effects of Ministry of Education， Anhui Agricultural University Bioactivity & Applications Lab， Department of Biological Sciences， Faculty of Science and engineering， University of Limerick

This work aims to compare the chemical composition and anti-inflammatory effects on RAW264.7 macrophages of Keemun black tea stems and leaves. Fifty volatile compounds were identified in tea stems and leaves， and aldehydes， alcohols， and esters were the main volatile compound categories. Eleven key volatile compounds， including geraniol， phenylacetaldehyde， methyl salicylate， linalool， etc. contributed to distinguishing the tea stems from the tea leaves. In the quantitative and LC-MS-based metabolomics analysis， higher contents of amino acids， monosaccharides， and quinic acid were found in stems than in leaves. Inversely， higher contents of tea pigments， flavan-3-ols， gallic acid， purine alkaloids， and flavonol glycosides were present in tea leaves than in stems. LC-MS-based metabolomics also revealed that organic acids were the most critical non-volatile compounds responsible for the differences between tea stems and leaves. Furthermore， tea stems had better inhibiting effects of pro-inflammatory cytokines (IL-1β and IL-6) in lipopolysaccharide-challenged RAW264.7 macrophages than tea leaves， while no significant differences exist between leaves and stems for inhibiting the secretion of TNF-α and NO. In conclusion， our results support using Keemun black tea stems as a novel source of anti-inflammatory compounds.

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Comparison of Five Endogenous Reference Genes for Specific PCR Detection and Quantification of Rice

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Rice science 2019年第4期26卷 248-256,I0006,I0007页

作者： ZHANG Xiujie JIN Wujun XU Wentao LI Xiaying SHANG Ying LI Sha OUYANG Hongsheng College of Animal Sciences Jilin University Changchun 130062 China Development Center of Science and Technology Ministry of Agriculture and Rural Affairs Beijing 100176 China Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081China College of food Science and Nutritional engineering China Agricultural University Beijing 100083China

Endogenous reference genes (ERGs) provide vital information regarding genetically modified organisms (GMOs). The successful detection of ERGs can identity GMOs and the source of genes, verify stability and reliability of the detection system, and calculate the level of genetically modified (GM) ingredients in mixtures. The reported ERGs in rice include sucrose-phosphate synthase (SPS), phospholipase D (PLD), RBE4 and rice root-specific GOS9 genes. Based on the characteristics of ERGs, a new ERG gene, phosphoenolpyruvate carboxylase (PEPC), was selected, and further compared with the four existing genes. A total of 18 rice varieties and 29 non-rice crops were used to verify the interspecies specificity, intraspecies consistency, sensitivity, stability and reliability of these five ERGs using qualitative and quantitative PCR. Qualitative detection indicated that SPS and PEPC displayed sufficient specificity, and the detection sensitivity was 0.05% and 0.005%, respectively. Although the specificity of both RBE4 and GOS9 were adequate, the amplicons were small and easily confused with primer dimers. Non-specific amplification of the PLD gene was present in maize and potato. Real-time quantitative PCR detection indicated that PLD, SPS and PEPC displayed good specificity, with R2 of the standard curve greater than 0.98, while the amplification efficiency ranged between 90% and 110%. Both the detection sensitivities of PLD and PEPC were five copies and that of SPS was ten copies. RBE4 showed typical amplification in maize, beet and Arabidopsis, while GOS9 was found in maize, tobacco and oats. PEPC exhibited excellent detection sensitivity and species specificity, which made it a potentially useful application in GM-rice supervision and administration. Additionally, SPS and PLD are also suitable for GM-rice detection. This study effectively established a foundation for GMO detection, which not only provides vital technical support for GMO identification, but also is of grea

关键词： endogenous reference gene rice genetically modified crop phosphoenolpyruvate carboxylase gene sucrose-phosphate synthase gene phospholipase D gene starch branching enzyme 4 gene rice root-specific GOS9 gene

Expedited extraction of xylan from corncob by power ultrasound

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International Journal of Agricultural and Biological engineering 2009年第4期2卷 76-83页

作者： Wade Yang Vishnu K.Ajapur Kathiravan Krishnamurthy Hao Feng Ruijin Yang Taha M.Rababah Department of food Science and Human Nutrition University of FloridaGainesvilleFL 32611USA Department of food and Animal Sciences Alabama A&M UniversityNormalAL 35762USA Illinois Institute of Technology Summit-ArgoIL 60501USA Department of food Science and Human Nutrition University of Illinois at Urbana-ChampaignUrbanaIL 61801USA School of food Science and Technology Southern Yangtze UniversityWuxi 214036China Department of food Science and Human Nutrition Jordan University of Science and TechnologyIrbid22110Jordan

Some lignocellulosic biomass like corncob contains a large amount of xylan,a valuable material for applications in food,chemical and pharmaceutical *** extraction of xylan is a time consuming process,requiring 12 to 24 hours to *** this study,the efficacy of power ultrasound for reducing the extraction time and increasing the xylan yield was *** acoustic energy densities(0.18 W/mL and 0.45 W/mL)of the power ultrasound were applied to corncob for 10,20,30,and 60 *** treated samples were steamed at 110,118 and 121℃for 30 *** 0.45 W/mL acoustic energy density and 121℃steaming temperature,ultrasonic treatment was able to extract 39%of xylan in 43 *** contrast,the conventional treatment was able to extract only 34%of xylan in 24 *** ultrasound assisted extraction reduced the processing time by 97%in addition to yield increase by 14.7%.Power ultrasound can be a potential technology for enhanced xylan extraction from corncob to result in significant processing time savings.

关键词： Power ultrasound sonication lignocellulusic biomass xylan extraction corncob steaming