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Modeling method for SSC prediction in pomelo using Vis-NIRS with wavelength selection and latent variable updating

International Journal of Agricultural and Biological engineering 2024年第1期17卷 251-260页

作者： Hao Tian Shuai Wang Huirong Xu Yibin Ying College of Biosystems engineering and food Science Zhejiang UniversityHangzhou 310058China Key Laboratory of On-Site Processing Equipment for Agricultural Products Ministry of Agriculture and Rural AffairsHangzhou 310058China College of food Science Shihezi UniversityShihezi 832003XinjiangChina

The aim of this study was in-line,rapid,and non-destructive detection for soluble solid content(SSC)in pomelos using visible and near-infrared spectroscopy(Vis-NIRS).However,the large size and thick rind of pomelo affect the stability of spectral acquisition and the biological variabilities affect the robustness of *** these issues,in this study,an efficient prototype in-line detection system in transmittance mode was designed and evaluated in comparison with an off-line detection *** from the years 2019 and 2020 were used for modeling and the external validation data were obtained by the inline detection system in *** wavelength selection methods of changeable size moving window(CSMW),random frog(RF),and competitive adaptive reweighted sampling(CARS)were used to improve the prediction accuracy of partial least squares regression(PLSR)*** best performance of internal prediction was obtained by CARS-PLSR and the determination coefficient of prediction(),root mean square error of prediction(RMSEP),and residual predictive deviation(RPD)were 0.958,0.204%,and 4.821,***,all models obtained large prediction biases in external *** latent variable updating(LVU)method was proposed to update models and improve the performance in external *** samples from the external validation set were randomly selected to update the *** with the recalibration method,LVU could effectively modify the original models which matched the SSC range of the external validation *** CSMW-PLSR models were more robust in external *** off-line model with LVU performed best with a root mean square error of validation(RMSEV)of 0.599%and the in-line model with recalibration obtained RMSEV of 0.864%.These results demonstrated the application potential of the transmittance Vis-NIRS for in-line rapid prediction of SSC in pomelos and the modeling and updating methods could be applied to samples with biological variabi

关键词： Vis-NIRS in-line detection external validation wavelength selection model updating pomelo SSC

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Chlorantraniliprole exposure aggravates high-fat diet-induced metabolic disorders in mice by regulating gut microbiota and its metabolites

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food Science and Human Wellness 2024年

作者： Ting Gu Jie Lin Jiani Yang William Mumby Quancai Sun Ye Peng Faculty of Medicine Macau University of Science and Technology School of food and Biological engineering Jiangsu University Department of Health Nutrition and food Sciences Florida State University

food contaminants， particularly insecticides， are important factors contributing to obesity and other adverse effects. As the most widely used diamide insecticide worldwide， chlorantraniliprole (CP) is ubiquitous in food and the environment. However， the influence of CP on obesity and the gut microbiota remains unknown. In this study， we administered CP/carboxymethyl cellulose sodium to C57BL/6J mice with a high-fat diet (HFD) via gavage for 13 weeks. The CP exposure induced significant increases in body weight gain， fat mass， serum total cholesterol (TC)， and low-density lipoprotein cholesterol (LDL-C). Moreover， CP aggravated the imbalance in the gut microbiota by increasing the relative abundance of obesity-associated bacteria and reducing the relative abundance of beneficial bacteria. Based on untargeted metabolomics analysis， CP was found to be involved in the regulation of pathways including ‘alanine， aspartate and glutamate metabolism’ and ‘arginine and proline metabolism’. Notably， CP exposure primarily induced alterations in microbial metabolites within these pathways， such as L-(+)-aspartic acid and L-glutamic acid. Additionally， individual metabolites， such as the lipid mediator (oleoyl ethanolamide)， also demonstrated alterations upon CP exposure. Furthermore， Spearman correlation analysis revealed several noteworthy associations between microbial alterations， metabolite changes， and phenotypes. The results of the study demonstrate a connection between microbiota， metabolites， and the effects of CP exposure on HFD-induced obesity， elucidating the critical role of the gut microbiota and its metabolites in the toxic effects of CP.

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Trimethylamine N-oxide generation process was influenced by the proportion and source of macronutrients in the diet

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food Science and Human Wellness 2024年第2期13卷 649-658页

作者： Chengcheng Wang Xuefeng Duan Xiaoyue Li Jinyue Yang Changhu Xue Teruyoshi Yanagita Tiantian Zhang Yuming Wang College of food Science and engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Laboratory of Nutrition Biochemistry Department of Applied Biochemistry and Food ScienceSaga UniversitySaga 840-8502Japan

Trimethylamine N-oxide(TMAO)is a risk factor of various chronic diseases,which was produced by metabolism from precursors to trimethylamine(TMA)in gut and the oxidation from TMA in *** TMA generation was influenced by diet,mainly due to the rich TMAO precursors in ***,it was still unclear that the effects of different proportion and source of macronutrients in different dietary pattern on the production process of ***,the generation of TMA from precursors and TMAO from TMA was determined after single oral choline chloride and intraperitoneal injection TMA,respectively,in mice fed with carbohydrates,proteins and fats in different proportion and *** results suggested that the generation of TMAO was increased by low non-meat protein and high fat via enhancing the production of TMAO from TMA,and decreased by plant protein and refined sugar via reducing TMA production from precursors in gut and TMAO transformation from TMA in *** high fat and high sugar diets accelerating the development of atherosclerosis did not increase the production of TMAO,the risk factor for atherosclerosis,which indicated that the dietary compositions rather than the elevated TMAO level might be a more key risk factor for atherosclerosis.

关键词： Trimethylamine N-oxide(TMAO) Trimethylamine(TMA) Dietary composition Macronutrients Gut microbiota

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Biochemistry and transcriptome analysis reveal condensed tannins alleviate liver injury induced by regulating cholesterol metabolism pathway

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food Science and Human Wellness 2024年第2期13卷 909-919页

作者： Xiangxin Li Yijing Pu Bangdi Liu Xiaoming Fang Wenjun Peng Weibo Jiang Wenli Tian Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China College of food Science and Nutritional engineering China Agricultural UniversityBeijing 100083China Academy of Agricultural Planning and engineering Ministry of Agriculture and Rural AffairsBeijing 100125China

Free cholesterol has been considered to be a critical risk factor of nonalcoholic fatty liver disease(NAFLD).It remains unknown whether dietary intake of condensed tannins(CTs)have distinguishable effects to alleviate liver damage caused by a high cholesterol *** C57BL/6 mice were fed a high cholesterol diet for 6 weeks,and given CTs treatment at a dosage of 200 mg/(kg·day)at the same *** results indicated that compared with mice fed a normal diet,a high cholesterol diet group resulted in significant weight loss,dysregulation of lipid metabolism in blood and liver,and oxidative stress in the liver,but CTs treatment dramatically reversed these negative *** and eosin(H&E)staining and frozen section observation manifested that CTs treatment could effectively reduce the deposition of liver cholesterol and tissue necrosis caused by high cholesterol *** alleviated liver injury mainly by regulating the expression of related genes in cholesterol metabolism pathway and AMPK *** results confirmed that CTs have remarkable cholesterol lowering and anti-liver injury effects in vivo.

关键词： Condensed tannins High cholesterol Liver injury Antioxidants Transcriptomic analysis

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Effect of wall-disruption on nutrient composition and in vitro digestion of camellia and lotus bee pollens

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food Science and Human Wellness 2024年第3期13卷 1567-1577页

作者： Yuan Yuan Shun Zhong Zeyuan Deng Guangyan Li Jinwu Zhang Hongyan Li State Key Laboratory of food Science and Resources Nanchang UniversityNanchang 330047China Nanchang Tongxin Zichao Biological engineering Co. Ltd.Nanchang 330052China

The nutrient digestion,absorption and biological activity of bee pollen may be limited due to the complex pollen ***,the effect of superfine grinding technology on the release of nutrients from bee pollen were investigated,and their antioxidant activities and in vitro digestion were explored in this *** showed that the content of nutrients in bee pollen increased after wall *** them,fat content increased by 22.55%-8.31%,protein content increased by 0.54%-4.91%,starch content increased by 36.31%-48.64%,soluble sugar content increased by 20.57%-29.67%,total phenolic acid content increased by 11.73%-86.98%and total flavonoids content increased by 14.29%-24.79%.At the same time,the antioxidant activity increased by 14.84%-46.00%.Furthermore,the active components such as phenolic compounds in the wall-disruption bee pollen were more readily to be released during the in vitro digestion,and easier to be absorbed because of their higher *** activities during in vitro digestion were also improved in walldisruption bee *** findings provide evidence that bee pollen wall disruption was suggested,thus,it is more conducive to exerting the value of bee pollen in functional foods.

关键词： Bee pollen Nutrients Wall disruption Phenolic compounds In vitro digestion

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Gel-Based Triboelectric Nanogenerators for Flexible Sensing:Principles,Properties,and Applications

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Nano-Micro Letters 2024年第10期16卷 257-303页

作者： Peng Lu Xiaofang Liao Xiaoyao Guo Chenchen Cai Yanhua Liu Mingchao Chi Guoli Du Zhiting Wei Xiangjiang Meng Shuangxi Nie School of Light Industry and food engineering Guangxi UniversityNanning 530004People's Republic of China Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 101400People's Republic of China

The rapid development of the Internet of Things and artificial intelligence technologies has increased the need for wearable,portable,and self-powered flexible sensing *** nanogenerators(TENGs)based on gel materials(with excellent conductivity,mechanical tunability,environmental adaptability,and biocompatibility)are considered an advanced approach for developing a new generation of flexible *** review comprehensively summarizes the recent advances in gel-based TENGs for flexible sensors,covering their principles,properties,and *** on the development requirements for flexible sensors,the working mechanism of gel-based TENGs and the characteristic advantages of gels are *** strategies for the performance optimization of hydrogel-,organogel-,and aerogel-based TENGs are systematically *** addition,the applications of gel-based TENGs in human motion sensing,tactile sensing,health monitoring,environmental monitoring,human-machine interaction,and other related fields are ***,the challenges of gel-based TENGs for flexible sensing are discussed,and feasible strategies are proposed to guide future research.

关键词： Triboelectric nanogenerators Gel materials Triboelectric materials Flexible sensing

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CRISPR/Cas9-mediated NlInR2 mutants:Analyses of residual mRNA and truncated proteins

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Journal of Integrative Agriculture 2024年第6期23卷 2006-2017页

作者： Jun Lü Jingxiang Chen Yutao Hu Lin Chen Shihui Li Yibing Zhang Wenqing Zhang State Key Laboratory of Biocontrol/School of Life Sciences Sun Yat-sen UniversityGuangzhou 510275China Development of food Science and engineering Moutai InstituteRenhuai 564507China

CRISPR/Cas9 technology is a powerful genome manipulation tool in ***,little is known about whether mRNA and protein of a target gene are completely cleared in homozygous *** study generated homozygous mutants of the insulin receptor gene 2(NlInR2)in the brown planthopper(Nilaparvata lugens)using CRISPR/Cas9 genome *** frameshift mutants,E5_D17 and E6_I7,differentiated towards long wings,but there were differences in wing morphology,with E5_D17 showing wing *** investigations revealed the presence of residual expression of NlInR2 mRNA in both mutants,as well as the occurrence of spliceosomes featuring exon skipping splicing in E5_***,the E5_D17 exhibited the detection of N-terminally truncated NlInR2 *** interference experiments indicated that the knockdown of NlInR2 expression in the E5_D17 mutant line increased the proportion of wing deformities from 11.1 to 65.6%,suggesting that the residual NlInR2 mRNA of the E5_D17 mutant might have retained some genetic *** results imply that systematic characterization of residual protein expression or function in CRISPR/Cas9-generated mutant lines is necessary for phenotypic interpretation.

关键词： CRISPR/Cas9 Nilaparvata lugens residual mRNA skipping exon truncated protein

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Research progress on classification,source,application of phytosterol esters,and their thermal oxidation stability

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Grain & Oil Science and Technology 2024年第1期7卷 1-11页

作者： Dami Li Shangde Sun Jingnan Chen School of food Science and engineering Henan University of TechnologyZhengzhou 450001Henan ProvinceChina Henan engineering Research Center of Oil Seed Deep Processing Henan University of TechnologyZhengzhou 450001Henan ProvinceChina

Phytosterol esters can effectively decrease serum cholesterol concentration in the human body and prevent cardio-cerebrovascular *** was found that phytosterol esters exhibited better solubility and bioavailability than free *** recent years,phytosterol esters have attracted increasing ***,during food processing,phytosterol esters are susceptible to degradation at high temperatures,resulting in certain losses and formation of potentially harmful substances for *** paper reviews the relevant literatures and updates on the thermal oxidation stability of phytosterol esters in recent years from the following aspects:(i)Sources,physiological activities,and applications of phytosterol esters;(ii)Oxidation mechanism of phytosterol esters;(iii)Effects of phytosterols species,the volume of addition,food matrix,heating temperature and time,and antioxidants on the thermal loss and oxidation stability of phytosterol *** research progress on the safety of phytosterol esters is also discussed in ***,the prospects for future research are highlighted.

关键词： Thermal stability Loss rate Oxidation mechanism Phytosterol esters

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Microbial Growth and Decay: A Commented Review of the Model

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Advances in Microbiology 2024年第1期14卷 1-10页

作者： Alberto Schiraldi Formerly at dept. food Environ. Nutr. Sci. (DeFENS) University of Milan (Italy) Milan Italy

The paper reviews previous publications and reports some comments about a semi empirical model of the growth and decay process of a planktonic microbial culture. After summarizing and reshaping some fundamental mathematical expressions, the paper highlights the reasons for the choice of a suitable time origin that makes the parameters of the model self-consistent. Besides the potential applications to predictive microbiology studies and to effects of bactericidal drugs, the model allows a suitable proxy of the fitness of the microbial culture, which can be of interest for the studies on the evolution across some thousand generations of a Long Term Evolution Experiment.

关键词： Microbial Cultures Model Time Scale Growth and Decay Evolution

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Sea cucumber gut tetrapeptide ameliorates alcoholic gastric damage via restoring mitochondrial dynamics

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food Science and Human Wellness 2024年

作者： Zhihong Zheng Na Sun Jingqi Yang Zhijie Bao Songyi Lin State Key Laboratory of Marine food Processing and Safety Control School of food Science and Technology Dalian Polytechnic University National engineering Research Center of Seafood School of food Science and Technology Dalian Polytechnic University Collaborative Innovation Center of Seafood Deep Processing Dalian Polytechnic University Liaoning engineering Research Center of Special Dietary food Dalian Polytechnic University

This paper aimed to explore the mechanism of tetrapeptide VTPY in improving alcoholic gastric injury. VTPY has the potential to enhance the growth and movement of normal GES-1 cells. Following ethanol-induced impairment， VTPY effectively improved migration of GES-1 and HUVEC cells， enhanced angiogenesis， eliminated cellular and mitochondrial ROS， inhibited excessive mitochondrial division， enhanced F-actin polymerization and mitochondrial respiratory capacity. To counteract excessive mitochondrial fission， VTPY primarily restores the mitochondria dynamics by reducing the expression of Drp1 and Fis1， while increasing Mfn2. Further studies utilizing inhibitors clarifies that the inhibition of excessive mitochondrial fission can markedly reduce F-actin depolymerization， consequently enhancing cell migration. Additionally， VTPY can inhibit the apoptosis pathway by maintaining potential of mitochondrial membrane， preventing the release of mitochondrial cytochrome c， bolstering the levels of Bcl-XL， while reducing the levels of Bax and cleaved-caspase3. Further investigations using inhibitors demonstrates that excessive mitochondrial fission could activate apoptotic pathway. However， VTPY counteracts this effect and enhance cells viability. Further evidence suggests that VTPY effectively improves ulcer index and pathologic changes， relieves inflammation， enhances the balance of oxidation and anti-oxidation， promotes angiogenesis， improves the expression of mitochondrial dynamics factors， blocks apoptotic pathway， and subsequently ameliorates gastric damage in mice through Fis-1/Bcl-2 pathway.

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