检索结果-南通市图书馆

Quantitative and qualitative subgenomic RNA profiles of SARS-CoV-2 in respiratory samples: A comparison between Omicron BA.2 and non-VOC-D614G

引用

Virologica Sinica 2024年第2期39卷 218-227页

作者： Zigui Chen Rita Way Yin Ng Grace Lui Lowell Ling Agnes S.Y.Leung Chit Chow Siaw Shi Boon Wendy C.S.Ho Maggie Haitian Wang Renee Wan Yi Chan Albert Martin Li David Shu Cheong Hui Paul Kay Sheung Chan Department of Microbiology Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of medicine and Therapeutics Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Anaesthesia and Intensive Care Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Paediatrics Faculty of MedicineThe Chinese University of Hong KongHong KongChina Department of Anatomical and Cellular pathology Faculty of MedicineThe Chinese University of Hong KongHong KongChina Jockey Club School of Public Health and Primary Care Faculty of MedicineThe Chinese University of Hong KongHong KongChina Stanley Ho Centre for Emerging Infectious Diseases Faculty of MedicineThe Chinese University of Hong KongHong KongChina

The SARS-CoV-2 Omicron variants are notorious for their transmissibility,but little is known about their subgenomic RNA(sgRNA)*** study applied RNA-seq to delineate the quantitative and qualitative profiles of canonical sgRNA of 118 respiratory samples collected from patients infected with Omicron BA.2 and compared with 338 patients infected with non-variant of concern(non-VOC)-D614G.A unique characteristic profile depicted by the relative abundance of 9 canonical sgRNAs was reproduced by both BA.2 and non-VOCD614G regardless of host gender,age and presence of ***,such profile was lost in samples with low viral load,suggesting a potential application of sgRNA pattern to indicate viral activity of individual patient at a specific time point.A characteristic qualitative profile of canonical sgRNAs was also reproduced by both BA.2 and *** presence of a full set of canonical sgRNAs carried a coherent correlation with crude viral load(AUC¼0.91,95%CI 0.88–0.94),and sgRNA ORF7b was identified to be the best surrogate marker allowing feasible routine application in characterizing the infection status of individual *** potentials in using sgRNA as a target for vaccine and antiviral development are worth pursuing.

关键词： Coronavirus SARS-CoV-2 Omicron BA.2 Non-VOC-D614G RNA-seq Subgenomic RNA(sgRNA)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuroprotective effect of liraglutide and memantine in a rat model of Alzheimer’s disease

引用

Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 207-214页

作者： Duaa M.Bakhshwin Foziya W.Alhalabi Mohamad Omar Barasheed Ahmed M.Bakhshwin Maha H.Jamal Mohammed A.Bazuhair Fatemah O.Kamel Rania M.Magadmi Zoheir A Damanhouri Samar M.Alsaggaf Department of Clinical Pharmacology Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Hera General Hospital MakkahSaudi Arabia Department of pathology Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Clinical Anatomy Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia

Objective:To assess the effect of memantine combined with liraglutide on aluminum chloride(AlCl_(3))and D-galactose(D-GAL)-induced neurotoxicity in ***:Male Wistar rats were divided into 5 groups of 5 animals each:the positive control,the negative control,the memantine-treated group,the liraglutide-treated group,and the combination group treated with memantine and ***_(3)and D-GAL were used to induce *** tests,brain beta-amyloid protein,and oxidative stress biomarkers were ***:The Morris water maze test indicated an enhanced memory in the combination ***,the combination treatment of liraglutide and memantine resulted in a remarkable reduction in the beta-amyloid protein level in the brain *** inflammation and oxidative stress biomarkers were significantly reduced,and the levels of antioxidant parameters were ***:The combination of liraglutide and memantine exerts neuroprotective effects and enhances memory and cognitive functions in rats with Alzheimer’s disease.

关键词： Alzheimer’s disease Cognitive function Glucagonlike peptide-1 Liraglutide Memantine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Childhood asthma biomarkers including zinc: An exploratory crosssectional study

引用

World Journal of Clinical Pediatrics 2024年第1期13卷 31-40页

作者： Hoda Atef Abdelsattar Ibrahim Mona Mohsen Boles Salep Aziz Hanna Dina Mahmoud Khaled Mohamed Abdelhamid El-Khashab Pediatric Clinical Nutrition Unit Faculty of MedicineCairo UniversityCairo 12613Egypt Department of Pediatrics Faculty of MedicineCairo UniversityCairo 12613Egypt Department of Clinical pathology Faculty of MedicineCairo UniversityCairo 12613Egypt

BACKGROUND Childhood bronchial asthma(BA)is a chronic inflammatory respiratory *** conditions,including zinc deficiency,can affect such allergic *** To outline the difference in serum zinc levels between asthmatic children and healthy *** A cross-sectional study was carried out at Children’s Hospital,Cairo university,investigating serum zinc levels in children with BA(n=40)and healthy children(n=21).Other markers included serum ferritin,iron,hemoglobin(Hb),and immunoglobulin E(IgE)*** t-tests and Mann-Whinny tests were used for *** Kruskal-Wallis test was applied to compare serum ferritin and IgE levels with regard to asthma ***'s rank correlation was performed to explore the relationship between serum ferritin levels and both iron and Hb levels in asthmatic *** Children with BA had higher levels of zinc,yet the difference was not significant(P=0.115).Serum ferritin and IgE levels were significantly higher in asthmatic children(P=0.006 and 0.001,respectively),yet their levels did not differ significantly by severity(P=0.623 and 0.126,respectively).There was a nonsignificant weak correlation between serum ferritin levels and both serum iron and Hb *** Serum zinc levels do not seem to differ between asthmatic children and healthy *** ferritin levels may be a marker of asthma *** IgE levels are not markers of asthma severity.

关键词： Children Asthma Zinc Ferritin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The interplay mechanism between IDH mutation, MGMT-promoter methylation, and PRMT5 activity in the progression of grade 4 astrocytoma: unraveling the complex triad theory

引用

Oncology Research 2024年第6期32卷 1037-1045页

作者： MAHER KURDI ALAA ALKHOTANI ABDULRAHMAN SABBAGH EYAD FAIZO AHMED I.LARY AHMED K.BAMAGA MAJID ALMANSOURI BADR HAFIZ THAMER ALSHARIF SALEH BAEESA Department of pathology Faculty of MedicineKing Abdulaziz UniversityRabighSaudi Arabia Department of pathology College of MedicineUmm Al-Qura UniversityMakkahSaudi Arabia Department of Surgery Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Surgery Faculty of MedicineUniversity of TabukTabukSaudi Arabia Section of Neurosurgery Department of SurgeryKing Abdulaziz Medical CityJeddahSaudi Arabia Department of Pediatrics Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Clinical Biochemistry Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Neurosciences King Faisal Specialist Hospital and Research CenterJeddahSaudi Arabia Department of Surgery King Abdulaziz Specialist HospitalTaifSaudi Arabia

Background:The dysregulation of Isocitrate dehydrogenase(IDH)and the subsequent production of 2-Hydroxyglutrate(2HG)may alter the expression of epigenetic proteins in Grade 4 *** interplay mechanism between IDH,O-6-methylguanine-DNA methyltransferase(MGMT)-promoter methylation,and protein methyltransferase proteins-5(PRMT5)activity,with tumor progression has never been ***:A retrospective cohort of 34 patients with G4 astrocytoma is classified into IDH-mutant and IDH-wildtype *** groups were tested for MGMT-promoter methylation and PRMT5 through methylation-specific and gene expression PCR ***-cohort statistical significance was ***:Both IDH-mutant WHO grade 4 astrocytomas(n=22,64.7%)and IDH-wildtype glioblastomas(n=12,35.3%)had upregulated PRMT5 gene expression except in one *** of the 22 IDH-mutant tumors,10(45.5%)tumors showed MGMT-promoter methylation and 12(54.5%)tumors had unmethylated *** IDH-wildtype tumors had unmethylated *** was a statistically significant relationship between MGMT-promoter methylation and IDH in G4 astrocytoma(p-value=0.006).Statistically significant differences in progression-free survival(PFS)were also observed among all G4 astrocytomas that expressed PRMT5 and received either temozolomide(TMZ)or TMZ plus other chemotherapies,regardless of their IDH or MGMT-methylation status(p-value=0.0014).Specifically,IDH-mutant tumors that had upregulated PRMT5 activity and MGMT-promoter methylation,who received only TMZ,have exhibited longer ***:The relationship between PRMT5,MGMT-promoter,and IDH is not ***,accumulation of D2-hydroxyglutarate(2-HG),which partially activates 2-OG-dependent deoxygenase,may not affect their *** IDH-wildtype glioblastomas,the 2HG-2OG pathway is typically inactive,leading to PRMT5 *** alone,compared to TMZ-plus,can increase PFS in upregulated PRMT5 ***,using a PRMT5 inhibitor in G4

关键词： Grade 4 astrocytoma Glioblastoma Isocitrate dehydrogenase(IDH) O-6-methylguanine-DNA methyltransferase(MGMT) Protein methyltransferase proteins-5(PRMT5) Progression-free survival(PFS)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Levisticum officinale extract protects against CCl4-induced hepatotoxicity through anti-inflammatory,anti-fibrotic,and antioxidant properties in rats

引用

Asian Pacific Journal of Tropical Biomedicine 2024年第8期14卷 331-340页

作者： Nahid Ghaedi Iran Pouraboli Mansour Mirtadzadini Mohammad-mehdi Moeini-aghtaie Department of Biology Faculty of SciencesShahid Bahonar University of KermanKermanIran Department of pathology Pathology and Stem Cell Research CenterAfzalipour Medical FacultyKerman University of Medical SciencesKermanIran

Objective:To investigate the hepatoprotective effects of Levisticum officinale extract on CCl4-induced ***:Different doses of Levisticum officinale extract were given orally to rats for 10 days,then rats received a single dose of CCl4(2.5 mL/kg,50%v/v in liquid paraffin).Biochemical and histopathological assays were performed to assess the effects of the extract on liver function and ***,antioxidant and oxidative markers as well as inflammatory and fibrotic indicators were ***:Pretreatment with Levisticum officinale extract significantly mitigated CCl4-induced damage to liver structure,improved serum levels of alanine aminotransferase,aspartate aminotransferase,alkaline phosphatase,urea,total bilirubin,and total protein,enhanced glutathione content and superoxide dismutase and catalase activities in the liver,as well as decreased plasma and hepatic malondialdehyde *** results demonstrated that the extract reduced Ki-67 andα-SMA expression and Masson’s trichrome staining revealed decreased liver collagen in rats treated with Levisticum officinale ***,Levisticum officinale extract markedly decreased the gene expressions of TNF-α,IL-6,TGF-β1,MCP-1,and ***:Levisticum officinale extract exerts hepatoprotective effects on CCl4-induced hepatotoxicity through antioxidant,anti-inflammatory,and anti-fibrotic activities.

关键词： Levisticum officinale CCl4 Inflammation Liver toxicity Antioxidant

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neutrophils are shaped by the tumor microenvironment:novel possibilities for targeting neutrophils in cancer

引用

Signal Transduction and Targeted Therapy 2024年第5期9卷 1788-1790页

作者： Carlos Silvestre-Roig Lydia Kalafati Triantafyllos Chavakis Institute of Experimental pathology(ExPat) Center for Molecular Biology of Inflammation(ZMBE)University of MünsterMünsterGermany Institute for Clinical Chemistry and Laboratory medicine University Hospital and Faculty of MedicineTechnische Universität DresdenDresdenGermany

In a recent paper published in Science,Ng et *** seminal insights into the process through which neutrophils are reprogrammed by the tumor environment,leading to a convergent transition from different neutrophil states towards a unique end-stage differentiated pro-tumor phenotype.1 These findings have major translational implications as they provide potential neutrophil-related targets for tumor immunotherapy.

关键词： al. neutrophils tumor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Diverse genotypes of norovirus genogroup I and II contamination in environmental water in Thailand during the COVID-19 outbreak from 2020 to 2022

引用

Virologica Sinica 2024年第4期39卷 556-564页

作者： Kattareeya Kumthip Pattara Khamrin Aksara Thongprachum Rungnapa Malasao Arpaporn Yodmeeklin Hiroshi Ushijima Niwat Maneekarn Department of Microbiology Faculty of MedicineChiang Mai UniversityChiang MaiThailand Center of Excellence(Emerging and Re-emerging Diarrheal Viruses) Chiang Mai UniversityChiang MaiThailand faculty of Public Health Chiang Mai UniversityChiang MaiThailand Department of Community medicine Faculty of MedicineChiang Mai UniversityChiang MaiThailand Department of pathology and Microbiology Nihon University School of MedicineTokyoJapan

Noroviruses(NoVs)are the most significant viral pathogens associated with waterborne and foodborne outbreaks of nonbacterial acute gastroenteritis in humans *** study aimed to investigate the prevalence and diversity of NoVs contaminated in the environmental water in Chiang Mai,Thailand.A total of 600 environmental water samples were collected from ten sampling sites in Chiang Mai from July 2020 to December *** presence of NoV genogroups I(GI),GII,and GIV were examined using real-time RT-PCR *** genotype of the virus was determined by nucleotide sequencing and phylogenetic *** results showed that NoV GI and GII were detected at 8.5%(51/600)and 11.7%(70/600)of the samples tested,***,NoV GIV was not detected in this *** circulated throughout the year,with a higher detection rate during the winter *** NoV GI genotypes(GI.1-GI.6)and eight NoV GII genotypes(GII.2,GII.3,GII.7,GII.8,GII.10,GII.13,GII.17,and GII.21)were *** 121 NoV strains detected,GII.17 was the most predominant genotype(24.8%,30 strains),followed by GII.2(21.5%,26 strains),GI.3(17.4%,21 strains),and GI.4(16.5%,20 strains).Notably,NoV GII.3,GII.7,GII.8,and GII.10 were detected for the first time in water samples in this *** study provides insight into the occurrence and seasonal pattern of NoV along with novel findings of NoV strains in environmental water in Thailand during the COVID-19 *** findings emphasize the importance of further surveillance studies to monitor viral contamination in environmental water.

关键词： Detection Environmental water Norovirus(NoVs) Wastewater Thailand

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Study of the DNA Extraction from the Nail by Spin Column-based Nucleic Acid Purification

引用

Journal of Forensic Science and medicine 2024年第3期10卷 191-195页

作者： Kanchana Sujirachato Piya Wongyanin Pilaiwan Ramadjai Alisa Ladsuk Kingkan Pokkasap Wisarn Worasuwannarak Department of Medical Technology Faculty of Science and TechnologyBansomdejchaopraya Rajabhat UniversityBangkokThailand Department of pathology Faculty of Medicine Ramathibodi HospitalMahidol UniversityBangkokThailand

Background:Nails are one of the objects that are more durable than other witness *** study was interested in whether the Spin column-based nucleic acid purification technique could extract DNA from nails to give satisfactory *** and Objectives:This study aimed to evaluate the yield and quality of DNA extracted from the *** and Methods:Nail samples from 15 males and 15 females over 18 were extracted using GeneAll®Exgene™Cell SV Mini *** DNA concentration and purity were measured using a NanoDrop Spectrophotometer,and the quality of DNAwas assessed by polymerase chain reaction(PCR)using primers specific for human growth hormone(HGH).Results:The average yield of DNA was 0.816µg(0.141–2.706µg)obtained from the average nail weight of 30.2 mg(22.2–40.0 mg).The average DNA concentration was 27.2 ng/µL(4.7–90.2 ng/µL),corresponding to 30µL in *** all DNA samples(96.7%)had high purity(A260/A280≥1.80)and gave a band of PCR product of the HGH gene on agarose gel ***:Spin column-based nucleic acid purification is recommended for nail DNA extraction due to its simplicity and high quality.

关键词： DNA extraction nail DNA NanoDrop polymerase chain reaction spin column

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

HDPairFinder:A data processing platform for hydrogen/deuterium isotopic labeling-based nontargeted analysis of trace-level amino-containing chemicals in environmental water

引用

Journal of Environmental Sciences 2024年第2期136卷 583-593页

作者： Tingting Zhao Kristin Carroll Caley B.Craven Nicholas J.P.Wawryk Shipei Xing Jian Guo Xing-Fang Li Tao Huan Department of Chemistry Faculty of ScienceUniversity of British ColumbiaVancouver Campus2036 Main MallVancouverBritish ColumbiaV6T 1Z1Canada Division of Analytical and Environmental Toxicology Department of Laboratory Medicine and PathologyFaculty of Medicine and DentistryUniversity of AlbertaEdmontonAlberta T6G 2G3Canada

The combination of hydrogen/deuterium(H/D)formaldehyde-based isotopic methyl labeling with solid-phase extraction and high-performance liquid chromatography–high resolution mass spectrometry(HPLC-HRMS)is a powerful analytical solution for nontargeted analysis of trace-level amino-containing chemicals in water *** the huge amount of chemical information generated in HPLC-HRMS analysis,identifying all possible H/Dlabeled amino chemicals presents a significant challenge in data *** address this,we designed a streamlined data processing pipeline that can automatically extract H/D-labeled amino chemicals from the raw HPLC-HRMS data with high accuracy and ***,we developed a cross-correlation algorithm to correct the retention time shift resulting from deuterium isotopic effects,which enables reliable pairing of H-and D-labeled ***,we implemented several bioinformatic solutions to remove false chemical features generated by in-source fragmentation,salt adduction,and natural13C ***,we used a data mining strategy to construct the AMINES library that consists of over 38,000 structure-disjointed primary and secondary amines to facilitate putative compound ***,we integrated these modules into a freely available R program,*** rationale of each module was justified and its performance tested using experimental H/D-labeled chemical standards and authentic water *** further demonstrated the application of HDPairFinder to effectively extract N-containing contaminants,thus enabling the monitoring of changes of primary and secondary N-compounds in authentic water *** is a reliable bioinformatic tool for rapid processing of H/D isotopic methyl labeling-based nontargeted analysis of water samples,and will facilitate a better understanding of N-containing chemical compounds in water.

关键词： Isotope labeling Nontargeted analysis Reactive N-compounds HPLC-HRMS Source water

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Moringa oleifera aqueous extract on the growth performance,blood characteristics,and histological features of gills and livers in Nile tilapia

引用

Aquaculture and Fisheries 2024年第1期9卷 85-92页

作者： Mahamoud A.Emam Ramy M.Shourbela Waleed N.El-Hawarry Seham Y.Abo-Kora Fatma Abdel-Monem Gad Ashraf M.Abd El-latif Mahmoud A.O.Dawood Histology Department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Department of Animal Husbandry and Animal Wealth Development Faculty of Veterinary MedicineAlexandria UniversityAlexandria22758Egypt Pharmacology Department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Clinical pathology Department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Department of Aquatic Animal Diseases and Management Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Animal Production Department Faculty of AgricultureKafrelsheikh UniversityKafr El-Sheikh33511Egypt The Center for Applied Research on the Environment and Sustainability The American University in CairoCairo11835Egypt

Moringa oleifera is well known as a highly nutritious plant and a water purifier in fish *** fish has many impressive qualities making them very suitable for ***,this study aimed to evaluate the effect of supplementation of Moringa oleifera aqueous extract(MOAE)at varying concentrations(0,100,200,and 400 mg/kg diet for 90 days)on growth performance,hematological,and some biochemical parameters of Nile ***,histological changes and immunohistochemical expression of caspase 3 in gills and livers were *** enhancements(P<0.05)in the final weight,weight gain,specific growth rate,red blood cells,hemoglobin,packed cell volume,white blood cells,and total serum protein were observed in fish fed 200 mg MOAE/kg diet compared with other ***,a significant reduction(P<0.05)of liver and kidney function tests and non-significant changes in lipid profile were *** the other hand,fish fed 400 mg MOAE/kg diet revealed disturbance in the corresponding *** histological degenerative changes were detected in the gills and liver of fish fed 400 mg MOAE/kg *** comparison,fish fed 200 mg MOAE/kg diet showed the best histo-architectures of these organs compared with other *** immune expressions of caspase 3 were determined where gills and liver of fish fed 400 mg MOAE/kg diet revealed extensive number of positive caspase 3 cells indicating severe apoptotic ***,a dietary MOAE at 200 mg/kg diet is recommended to improve the growth performance,hematological parameters,liver and kidney functions in Nile ***,caution must be considered when using MOAE at a concentration over 200 mg/kg diet.

关键词： Growth performance Liver Gills Moringa oleifera Nile tilapia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：