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Corrigendum to “Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes (imPODs)” [Genes & Diseases 5 (2018) 137–149]

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Genes & Diseases 2023年第2期10卷 630-631页

作者： Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C. Haydon Hue H. Luu Tong-Chuan He Hua Gan departments of Nephrology Orthopaedic SurgeryCardiologyGeneral SurgeryPlastic Surgeryand Clinical Laboratory MedicineThe First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory Medicine and the Affiliated Hospitals of Chongqing Medical UniversityChongqing 400016China Department of Biochemistry and Molecular Biology China Three Gorges University School of MedicineYichangHubei 443002China Department of Clinical Laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330031China Key Laboratory of orthopaedic surgery of Gansu Province and the Department of orthopaedic surgery The Second Hospital of Lanzhou UniversityLanzhouGansu 730030China

The authors regret having an image assembly error in Figure 3A,in which the image for "imPOD Synaptopodin DAPl stain"groupwas erroneouslyduplicatedwiththe imagefrom the"tsPOD-33C SynaptopodinDAPIstain"*** confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and *** authors would like to apologize for any inconvenience caused.

关键词： Cor cytes glomerular

Development of a simplified and inexpensive RNA depletion method for plasmid DNA purification using size selection magnetic beads(SSMBs)

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Genes & Diseases 2021年第3期8卷 298-306页

作者： Xi Wang Ling Zhao Xiaoxing Wu Huaxiu Luo Di Wu Meng Zhan Jing Zhang Mikhail Pakvasa William Wagstaff Fang He Yukun Mao Yongtao Zhang Changchun Niu Meng Wu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Kelly Hynes Jason Strelzow Mostafa El Dafrawy Tong-Chuan He Hongbo Qi Zongyue Zeng Ministry of Education Key Laboratory of Diagnostic Medicine School of Laboratory and Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL60637USA departments of Gastrointestinal surgery Obstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Department of Burn and Plastic surgery West China Hospital of Sichuan UniversityChengduSichuan Province610041PR China Department of orthopaedic surgery The First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouGuangdong Province510405PR China departments of orthopaedic surgery and Neurosurgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei Province430072PR China Department of orthopaedic surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong Province266061PR China Department of Laboratory Diagnostic Medicine Chongqing General HospitalChongqing400021PR China Institute of Boneand Joint Research The departments of Orthopaedic Surgery and Obstetrics and GynecologyThe First and Second Hospitals of Lanzhou UniversityLanzhouGansuProvince730030PR China Department of orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei Province430022PR China Department of Spine surgery Second Xiangya HospitalCentral South UniversityChangshaHunan Province410011PR China

Plasmid DNA(pDNA)isolation from bacterial cells is one of the most common and critical steps in molecular cloning and biomedical *** all pDNA purification in-volves disruption of bacteria,removal of membrane lipids,proteins and genomic DNA,purifi-cation of pDNA from bulk lysate,and concentration of pDNA for downstream *** many liquid-phase and solid-phase pDNA purification methods are used,the final pDNA preparations are usually contaminated with varied degrees of host RNA,which cannot be completely digested by RNase *** develop a simple,cost-effective,and yet effective method for RNA depletion,we investigated whether commercially available size selection magnetic beads(SSMBs),such as Mag-Bind®TotalPure NGS Kit(or Mag-Bind),can completely deplete bacterial RNA in pDNA *** this proof-of-principle study,we demonstrated that,compared with RNase A digestion and two commercial plasmid affinity purification kits,the SSMB method was highly efficient in depleting contaminating RNA from pDNA *** transfection and bacterial colony formation assays revealed that pDNA purified from SSMB method had superior quality and integrity to pDNA samples cleaned up by RNase A digestion and/or commercial plasmid purification *** further demonstrated that the SSMB method completely depleted contaminating RNA in large-scale pDNA ***,the Mag-bind-based SSMB method costs only 5-10%of most commercial plasmid purification kits on a per sample ***,the reported SSMB method can be a valuable and inexpensive tool for the removal of bacterial RNA for routine pDNA preparations.

关键词： DNA transfection DNA vaccination Gene delivery Plasmid DNA purification RNA depletion Size selection magnetic beads

Notch signaling:Its essential roles in bone and craniofacial development

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Genes & Diseases 2021年第1期8卷 8-24页

作者： Mikhail Pakvasa Pranav Haravu Michael Boachie-Mensah Alonzo Jones Elam Coalson Junyi Liao Zongyue Zeng Di Wu Kevin Qin Xiaoxing Wu Huaxiu Luo Jing Zhang Meng Zhang Fang He Yukun Mao Yongtao Zhang Changchun Niu Meng Wu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Michael J.Lee Jennifer Moriatis Wolf Aravind Athiviraham Sherwin S.Ho Tong-Chuan He Kelly Hynes Jason Strelzow Mostafa El Dafrawy Russell R.Reid Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Pritzker School of Medicine University of ChicagoChicagoIL 60637USA Section of Plastic and Reconstructive surgery Department of SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA departments of orthopaedic surgery Gastrointestinal SurgeryObstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory and Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Department of Burn and Plastic surgery West China Hospital of Sichuan UniversityChengduSichuan610041PR China Department of orthopaedic surgery The First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouGuangdong510405PR China departments of orthopaedic surgery and Neurosurgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei430072PR China Department of orthopaedic surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong266061PR China Department of Laboratory Diagnostic Medicine Chongqing General HospitalChongqing400021PR China Institute of Bone and Joint Research and the Department of Orthopaedic SurgeryThe Second Hospitals of Lanzhou UniversityGansuLanzhou730030PR China Department of orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei430072PR China Department of Spine surgery Second Xiangya HospitalCentral South UniversityChangshaHunan410011PR China

Notch is a cellecell signaling pathway that is involved in a host of activities including development,oncogenesis,skeletal homeostasis,and much *** specifically,recent research has demonstrated the importance of Notch signaling in osteogenic differentiation,bone healing,and in the development of the *** craniofacial skeleton is complex and understanding its development has remained an important focus in *** this review we briefly summarize what recent research has revealed about Notch signaling and the current understanding of how the skeleton,skull,and face *** then discuss the crucial role that Notch plays in both craniofacial development and the skeletal system,and what importance it may play in the future.

关键词： Alagille syndrome Bone Craniofacial development Craniosynostosis Notch Oncogenesis Osteogenesis Spondylocostal dysosotosis

In vivo and in silico biodynamic studies of cruciate ligament injuries

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医用生物力学 2010年第S1期25卷 41-43页

作者： Scott Tashman Christopher Harner departments of orthopaedic surgery Mechanical Engineering & Materials Science Bioengineering University of Pittsburgh

Introduction Studies of musculoskeletal structure-function relationships and how they are affected by injuries have primarily relied on in vitro and in vivo methods. The limitations of in vitro studies continue to pos... 详细信息

关键词： PCL OpenSim

韧带和肌腱的生物力学和力学生物学研究（英文）

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医用生物力学 2016年第4期31卷 301-307页

作者：李宗明王慧聪胡流源 Hand Research Laboratory Departments of Biomedical Engineering Orthopaedic Surgeryand Physical Medicine and RehabilitationCleveland Clinic MechanoBiology Laboratory Department of Orthopaedic Surgery University of Pittsburgh Musculoskeletal Research Center Department of BioengineeringUniversity of Pittsburgh

国际韧带和肌腱研讨会(The International Symposium on Ligaments and Tendons,ISI&T)于2000年在美国佛罗里达州奥兰多市首次召开。研讨会的宗旨是引起对韧带和肌腱研究的重视,并为生物工程师、生物学家、临床医师提供一个可以分享、... 详细信息

国际韧带和肌腱研讨会(The International Symposium on Ligaments and Tendons,ISI&T)于2000年在美国佛罗里达州奥兰多市首次召开。研讨会的宗旨是引起对韧带和肌腱研究的重视,并为生物工程师、生物学家、临床医师提供一个可以分享、评论、讨论韧带和肌腱最新研究成果的论坛。从2000年起,国际韧带和肌腱研讨会已经开展了15届;每届研讨会上涌现了大量令人振奋的关于当前韧带和肌腱研究热点和未来挑战的讨论。多年来,韧带和肌腱领域内的研究数量大幅增加,研究质量不断提升。为纪念《医用生物力学》杂志创刊30周年,本文总结过去30年里韧带和肌腱研究的主要进展,包括组织力学、力学生物学、损伤与治愈机制、组织修复和再生。

关键词：韧带肌腱生物力学力学生物学国际韧带和肌腱研讨会

Current evidence on potential of adipose derived stem cells to enhance bone regeneration and future projection

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World Journal of Stem Cells 2021年第9期13卷 1248-1277页

作者： Quang Le Vedavathi Madhu Joseph M Hart Charles R Farber Eli R Zunder Abhijit S Dighe Quanjun Cui Department of orthopaedic surgery University of Virginia School of MedicineCharlottesvilleVA 22908United States orthopaedic surgery Research Thomas Jefferson UniversityPhiladelphiaPA 19107United States Center for Public Health Genomics University of VirginiaCharlottesvilleVA 22908United States departments of Public Health Sciences and Biochemistry and Molecular Genetics University of VirginiaCharlottesvilleVA 22908United States Department of Biomedical Engineering University of VirginiaCharlottesvilleVA 22908United States

Injuries to the postnatal skeleton are naturally repaired through successive stepsinvolving specific cell types in a process collectively termed “bone regeneration”.Although complex, bone regeneration occurs through a series of well-orchestratedstages wherein endogenous bone stem cells play a central role. In most situations,bone regeneration is successful;however, there are instances when it fails andcreates non-healing injuries or fracture nonunion requiring surgical or therapeuticinterventions. Transplantation of adult or mesenchymal stem cells (MSCs) definedby the International Society for Cell and Gene Therapy (ISCT) as CD105+-CD90+CD73+CD45-CD34-CD14orCD11b-CD79αorCD19-HLA-DR- is beinginvestigated as an attractive therapy for bone regeneration throughout the *** isolated from adipose tissue, adipose-derived stem cells (ADSCs), aregaining increasing attention since this is the most abundant source of adult stemcells and the isolation process for ADSCs is straightforward. Currently, there isnot a single Food and Drug Administration (FDA) approved ADSCs product forbone regeneration. Although the safety of ADSCs is established from their usagein numerous clinical trials, the bone-forming potential of ADSCs and MSCs, ingeneral, is highly controversial. Growing evidence suggests that the ISCT definedphenotype may not represent bona fide osteoprogenitors. Transplantation of bothADSCs and the CD105- sub-population of ADSCs has been reported to induce bone regeneration. Most notably, cells expressing other markers such as CD146,AlphaV, CD200, PDPN, CD164, CXCR4, and PDGFRα have been shown torepresent osteogenic sub-population within ADSCs. Amongst other strategies toimprove the bone-forming ability of ADSCs, modulation of VEGF, TGF-β1 andBMP signaling pathways of ADSCs has shown promising results. The U.S. FDAreveals that 73% of Investigational New Drug applications for stem cell-basedproducts rely on CD105 expression as the “positive” marker for adult stem cell

关键词： Mesenchymal stem cells Adipose-derived stem cells Endogenous stem cells Skeletal stem cells Bone regeneration

Bone Morphogenetic Protein (BMP) signaling in development and human diseases

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Genes & Diseases 2014年第1期1卷 87-105页

作者： Richard N.Wang Jordan Green Zhongliang Wang Youlin Deng Min Qiao Michael Peabody Qian Zhang Jixing Ye Zhengjian Yan Sahitya Denduluri Olumuyiwa Idowu Melissa Li Christine Shen Alan Hu Rex C.Haydon Richard Kang James Mok Michael J.Lee Hue L.Luu Lewis L.Shi The University of Chicago Pritzker School of Medicine ChicagoIL 60637USA Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA departments of orthopaedic surgery Medicineand Gynecologythe Affiliated Hospitals of Chongqing Medical UniversityChongqing 400016China School of Bioengineering Chongqing UniversityChongqingChina

Bone Morphogenetic Proteins(BMPs)are a group of signaling molecules that belongs to the Transforming Growth Factor-b(TGF-b)superfamily of *** discovered for their ability to induce bone formation,BMPs are now known to play crucial roles in all organ *** are important in embryogenesis and development,and also in maintenance of adult tissue *** knockout models of various components of the BMP signaling pathway result in embryonic lethality or marked defects,highlighting the essential functions of *** this review,we first outline the basic aspects of BMP signaling and then focus on genetically manipulated mouse knockout models that have helped elucidate the role of BMPs in development.A significant portion of this review is devoted to the prominent human pathologies associated with dysregulated BMP signaling.

关键词： BMP signaling Development Genetics Mouse knockout Pathogenesis Signal transduction

Corrigendum to ‘Modeling colorectal tumorigenesis using the organoids derived from conditionally immortalized mouse intestinal crypt cells (ciMICs)’ [Genes Dis 8 (2021) 814-826]

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Genes & Diseases 2023年第2期10卷 618-619页

作者： Xiaoxing Wu Zhaoxia Li Hongyu Zhang Fang He Min Qiao Huaxiu Luo Jing Zhang Meng Zhang Yukun Mao William Wagstaff Yongtao Zhang Changchun Niu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Rex C. Haydon Russell R. Reid Hue H. Luu Tong-Chuan He Ziwei Wang Houjie Liang Bing-Qiang Zhang Ning Wang departments of Gastrointestinal surgery Medicine/GastroenterologyAnd Obstetrics and GynecologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 606037USA Department of Oncology The PLA Rocket Force Characteristic Medical CenterBeijing 100088China departments of Burn & Plastic surgery West China Hospital of Sichuan UniversityChengduSichuan 610041China Department of orthopaedic surgery The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdong 510405China departments of orthopaedic surgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei 430071China Department of orthopaedic surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong 266000China Department of Clinical Laboratory Medicine Chongqing General Hospital Affiliated with the University of Chinese Academy of SciencesChongqing 400013China Ministry of Education Key Laboratory of Diagnostic Medicine And the School of Laboratory Diagnostic MedicineChongqing Medical UniversityChongqing 400016China Department of orthopaedics Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Spine surgery Second Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Oncology and Southwest Cancer Center Southwest HospitalArmy Medical UniversityChongqing 400038China

The authors regret that an image assembly(copy/paste)error in Figure 3D,in which the image for the organoid of"Primary MiCs"group was erroneously duplicated with an image of primary MICs that was previously *** corrected figure is shown *** shown in the corrected Figure 3D,this error does not adversely impact the conclusion of the original *** authors would like to apologise forany inconvenience caused.

关键词： Figure colorectal Cor

The Endplate Morphology Changes with Change in Biomechanical Environment Following Discectomy

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International Journal of Clinical Medicine 2013年第7期4卷 8-17页

作者： Aakash Agarwal Anand K. Agarwal Vijay K. Goel Engineering Center for orthopaedic Research Excellence (E-CORE) Departments of Bioengineering and Orthopaedic Surgery Colleges of Engineering and Medicine University of Toledo Toledo USA.

Bone is a dynamic structure and is known to respond to changes in the load over time, in accordance with Wolff’s law. It states that the bone changes its shape and internal architecture in response to stresses acting on it [1]. Therefore, any structural changes in the spine may lead to bone remodeling due to changes in the optimal stress pattern. The changes in apparent density and thickness of the endplates following discectomy of varying amounts were analyzed. The study design coupled a bone remodeling algorithm based on strain energy density theory of adaptive remodeling with an experimentally validated 3D ligamentous finite element model of the spine. The apparent density and thickness of the index level endplates decreased above and below the region of discectomy. On the other hand, these parameters showed increases at the remaining regions of the endplate. There were no correlations between the amount of nucleus removed and the average percentage changes in apparent density and thickness of endplate above and below the discectomy region. However, the average percentage changes in apparent density and thickness at endplate in the other region increased with increase in amount of nucleus removed. These predictions are in agreement with the clinical observations [2-6].

关键词： Bone Remodeling Spine Biomechanics Disc Herniation Discectomy Endplates Finite Element Study