检索结果-南通市图书馆

EFFECT OF ADENOVIRUS—MEDIATED p53 GENE TRANSFER ON APOPTOSIS AND RADIOSENSITIVITY OF HUMAN

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2003年第1期15卷 14-18页

作者： ZHANGShan－wen XIAOShao－wen 等 departmentofradiationoncology BeijingUniversitySchoolofOncologyBeijing100036

To evaluate the effect of adenovirus-mediated p53 gene(Adp53) on apoptosis and radiosensitivity of human gastric carcinoma cell lines.Methods:Recombinant adenovirus expressing wild-type p53 lines with different p53 genetic status.p53 protein expression was detected by immunohistochemistry assay and western blot assay.Cell survival was assessed using a clonogenic assay.TUNEL assay was used in determination of apoptosis.Four human gastric carcinoma cells infected with Adp53 were irradiated with 4Gy and cell cycle distribution and Sub-G1 peak were assayed by flow cytometry.Results:G2/M arrest,apoptosis and inhibition of tumor cell proliferation were induced by infection at Adp53 at 100 MOI which caused high transfer rate of wild-type p53 and strong expression of p53 protein in four human gastric carcinoma cells.The radio-enhancement ratio of Adp53 at 4Gy were3.0 for W cell,3.6 for M cell,2.2 for neo cell and 2.5 for 823 cell in vitro.Conclusion :This study demonstrated that Adp53 transfer increased cellular apoptosis and radiosensitivity of human gastric carcinoma cell lines in vitro independently on cellular intrinsic p53 status thus supporting the combination of p53 gene therapy with radiotherapy in clinical trials.

关键词： Gastric carcinoma Radiosensitivity Apoptosis Adenovirus-mediated p53 gene R735.2

Docetaxel inhibits SMMC-7721 human hepatocellular carcinoma cells growth and induces apoptosis

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2003年第4期9卷 696-700页

作者： Chang-XinGeng Zhao-ChongZeng Ji-YaoWang DepartmentofGastroenterology ZhongshanHospitalFudanUniversityShanghai200032China departmentofradiationoncology ZhongshanHospitalFudanUniversityShanghai200032China

AIM: To investigate the in vitro anti-hepatocellular carcinoma (HCC) activity of docetaxel against SMMC-7721 HCC cells and its possible mechanism.METHODS: The HCC cells were given different concentrations of docetaxel and their growth was measured by colony forming assay. Cell cycle and apoptosis were analyzed by flow cytometry and fluorescence microscopy (acridine orange/ethidium bromide double staining, AO/EB), as well as electronic microscopy. The SMMC-7721 HCC cell reactive oxygen species (ROS) and glutathione (GSH) were measured after given docetaxel.RESULTS: Docetaxel inhibited the hepatocellular carcinoma cells growth in a concentration dependent manner with IC505×10-10 M. Marked cell apoptosis and G2/M phase arrest were observed after treatment with docetaxel ≥10-8M.Docetaxel promoted SMMC-7721 HCC cells ROS generation and GSH deletion.CONCLUSION: Docetaxel suppressed the growth of SMMC7721 HCC cells in vitro by causing apoptosis and G2/M phase arrest of the human hepatoma cells, and ROS and GSH may play a key role in the inhibition of growth and induction of apoptosis.

关键词：多舍他西肝细胞癌化疗细胞凋亡 SMMC-7721细胞系

Patients with brain metastases from gastrointestinal tract cancer treated with whole brain radiation therapy:Prognostic factors and survival

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2004年第22期10卷 3345-3348页

作者： SusanneBartelt FelixMomm ChristianWeissenberger JohannesLutterbach departmentofradiationoncology UniversityofFreiburgFreiburgi.Br.Germany

AIM: To identify the prognostic factors with regard to survival for patients with brain metastasis from primary tumors of the gastrointestinal tract. METHODS: Nine hundred and sixteen patients with brain metastases, treated with whole brain radiation therapy (WBRT) between January 1985 and December 2000 at the Department of Radiation Oncology, University Hospital Freiburg, were analyzed retrospectively. RESULTS: Fifty-seven patients presented with a primary tumor of the gastrointestinal tract (esophagus: n=0, stomach: n=10, colorectal: n=47). Twenty-six patients had a solitary brain metastasis, 31 patients presented with multiple brain metastases. Surgical resection was performed in 25 patients. WBRT was applied with daily fractions of 2 Gray (Gy) or 3Gy to a total dose of 50Gy or 30Gy, respectively. The interval between diagnoses of the primary tumors and brain metastases was 22.6mo vs 8.0mo for patients with primary tumors of the colon/rectum vs other primary tumors, respectively (P<0.01, log-rank). Median overall survival for all patients with brain metastases (n=916) was 3.4mo and 3.2mo for patients with gastrointestinal neoplasms. Patients with gastrointestinal primary tumors presented significantly more often with a solitary brain metastasis than patients with other primary tumors (P<0.05, log-rank). In patients with gastrointestinal neoplasms (n=57), the median overall survival was 5.8 mo for patients with solitary brain metastasis vs 2.7mo for patients with multiple brain metastases (P<0.01, log-rank). The median overall survival for patients with a Karnofsky performance status (KPS) ≥70 was 5.5mo vs 2.1mo for patients with KPS <70 (P<0.01, log-rank). At multivariate analysis (Cox Model) the performance status and the number of brain metastases were identified as independent prognostic factors for overall survival. CONCLUSION: Brain metastases occur late in the course of gastrointestinal tumors. Pretherapeutic variables like KPS and the number of brain metastases have a profound influence on treatment out

关键词：移位变化大脑胃肠道癌肿瘤放射治疗残留物

Cytokine and Immuno-Gene Therapy for Solid Tumors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2005年第2期2卷 81-91页

作者： Chuan-YuanLi QianHuang Hsiang-FuKung departmentofradiationoncology DukeUniversityMedicalCenterDurhamNC27710USA CentralLaboratories No.1People＇sHospitalShanghaiJiaotongUniversityShanghaiChina CenterforEmergingInfectiousDiseases FacultyofMedicineChineseUniversityofHongKongShatinNewTerritoryHongKongChina

Despite recent progress in our understanding of cancer biology and in many areas of cancer treatment,the success rate for cancer therapy remains dismal.Immunotherapy for cancer has long been an exciting field for many cancer researchers due to the possibility to mobilize the body's own immune system to eradicate cancer not only locally but also systemically.Since its initial discovery,cytokine-based immunotherapy has been vigorously and extensively investigated for cancer treatment due to the perception of it as a relatively easily purifiable,injectable form of cancer treatment agent.However,so far most cytokine-based therapy trials have fallen short of expectations.One of main obstacles is the difficulty to achieve therapeutically relevant dosage in patients without generating excessive normal tissue toxicity.The emergence of novel gene therapy approach to deliver therapeutic cytokine to tumors locally generated great excitement since it has the potential of generating sustained high local concentration of immunostimulatory cytokine without raising the systemic levels of the cytokines,which is responsible for most of the observed toxicity.In this review,we will attempt to provide an overview of the field and discuss some of the problems associated with cytokine-based immuno-gene therapy and potential solutions. Cellular & Molecular Immunology.2005;2(2):81-91.

关键词： cytokine gene therapy cancer immunotherapy

Characterization of Ku-deficient cells derived from Ku70-/-andKu80-/-mice

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中国病理生理杂志 2000年第10期 167-168页

作者： Li Xiao-ling,Burgman P,Li L,Ouyang H,Ling C.C,and Li G.C DepartmentofMedicalPhysics!departmentofradiationoncology MemorialSloan-KetteringCancerCenter NewYork NY10O21 DepartmentofMedicalPhysics!departmentofradiationoncology MemorialSloan-KetteringCancerCenter NewYork NY10O21 DepartmentofMedicalPhysics!Department

Impact of simultaneous assay, the PCNA, cyclinDl, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer-possible incorporation into clinical TNM staging system

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第25期11卷 3823-3829页

作者： Shu-ChaiZhu RenLi Yu-XiangWang WeiFeng JuanLi RongQiu departmentofradiationoncology FourthHospitalHebeiMedicalUniversityShijiazhuang050011HebeiProvinceChina

AIM: The aim of the present study is to use immunohistochemical methods to investigate the clinical implications of tumor markers in esophageal squamous cell carcinoma and evaluate their impact on prognosis.METHODS: From November 1990 to December 1996, 47patients were treated with preoperative radiation followed by radical esophagectomy. All patients were confirmed pathologically as suffering from squamous cell carcinoma.Immunohistochemical stain was done for PCNA, cyclinD1 protein expression and DNA content analyzed by image cytometry. Kaplan-Meier method for single prognostic factor and log-rank test was used to test the significant difference. Cox stepwise regression model and prognosis index model were used for survival analysis with multiple prognostic factors.RESULTS: Radio-pathological change, T stage and N stage, as the traditional prognostic factors had statistical difference in 3-, 5- and 10-year survival rates. While, tumor cell proliferating marked PCNA, cyclinD1 and DNA content served as independent prognostic factors of esophageal carcinoma. There was definitely an identity between the single and multiple factor analyses. PI was more accurate to evaluate the prognosis of esophageal carcinoma.CONCLUSION: It is possible that tumor cell proliferating marked PCNA, cyclinD1 and DNA content would become the endpoints for evaluating the prognosis of esophageal carcinoma.

关键词： PCNA 免疫组织化学术前手术治疗食管癌放射疗法

Expression of a novel immunoglobulin gene SNC73 in human cancer and non-cancerous tissues

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2003年第5期9卷 1054-1057页

作者： Jian-BinHu ShuZheng Yong-ChuanDeng departmentofradiationoncology SirRunRunShawHospitalZhejiangUniversityMedicalCollegeHangzhou310016ZhejiangProvinceChina CancerInstitute ZhejiangUniversityHangzhou310009ZhejiangProvinceChina DepartmentofSurgicalOncology TheSecondAffiliatedHospitalofZhejiangUniversityMedicalCollegeHangzhou310009ZhejiangProvinceChina

AIM: To investigate the expression of immunoglobulin gene SNC73 in malignant tumors and non-cancerous normal tissues.METHODS: Expression level of SNC73 in tumors and non-cancerous tissues from the same patient was determined by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay (RT-PCR-ELISA) in 90 cases of malignant tumors, including colorectal cancer, gastric cancer, breast cancer, lung cancer and liver cancer, Analysis on the correlation of SNC73 expression with sex, age, site,grade of differentiation, depth of invasion, and metastases in colorectal cancer patients was made.RESULTS: Expression level of SNC73 in non-cancerous colorectal mucosa and colorectal cancerous tissues was 1.234±0.842 and 0.737±0.731, respectively (P0.05). No correlation was found between SNC73 expression level and various clinicopathological factors, including sex, age, site,grade of differentiation, depth of invasion and metastases of CRC patients.CONCLUSION: Down-regulation of SNC73 expression may be a relatively specific phenomenon in colorectal cancer.SNC73 is a potential genetic marker for the carcinongenesis of colorectal cancer. The relationship of SNC73 expression and carcinogenesis of colorectal cancer merits further study.

关键词：免疫球蛋白基因表达肿瘤 SNC73基因聚合酶链反应 ELISA

Comparison of conformal and intensity-modulated techniques for simultaneous integrated boost radiotherapy of upper esophageal carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2004年第8期10卷 1098-1102页

作者： Wei-HuaFu Lu-HuaWang Zong-MeiZhou Jian-RongDai Yi-MinHu Lu-JunZhao departmentofradiationoncology CancerInstitute(Hospital)PekingUnionMedicalCollegeChineseAcademyofMedicalSciencesBeijing10021China

To compare intensity-modulated radiotherapy (IMRT)with conformal radiotherapy (CRT) by investigating the doseprofiles of primary tumors, electively treated regions, andthe doses to organs at risk.

关键词：上食管癌放射疗法等角增强调节技术综合治疗影像学检查

Post-radiation survival time in hepatocellular carcinoma based on predictors for CT-determined, transarterial embolization and various other parameters

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第11期11卷 1697-1699页

作者： Ting-KaiLeung Chi-MingLee Li-KuoShen Hsi-ChiChen Yu-ChengKuo Jeng-FongChiou DepartmentofDiagnosticRadiology TaipeiMedicalUniversityHospitalTaipeiTaiwanChina departmentofradiationoncology TaipeiMedicalUniversityHospitalandWanFangHospitalTaipeiTaiwanChina

AIM: In this retrospective study of unresectable helatocellular carcinoma (HCC), we have investigated the efficacy of CTderived parameters, laboratory measurements, clinical assessment and associated transarterial embolization (TAE) as predictors of post-radiotherapy survival time.METHODS: Sixty-six patients diagnosed with unresectable HCC that had undergone radiotherapy at two medical university hospitals in Taipei were enrolled in the study.Using multivariant analysis, pre-treatment parameters including tumor number and CT confirmation of PVT and ascites were compared. Multivariant analysis was also used for comparison of the mean pretreatment values for laboratory measurements, including alpha-fetoprotein,direct/total bilirubin and GOT/GPT levels, and clinical history of chronic hepatitis across the three survival-time categories. The χ2 was used to test the significance of the relationship between survival time and TAE procedure.The Pvalues for the above tests were deemed statisticallysignificant where P＜0.05.RESULTS: Portal vein thrombosis (P = 0.032) and ascites (P＜0.05) were negative predictors of post-radiation survival time. Low-grade liver cirrhosis (A or B), lower tumor volume and low levels of AFT, GOT/GPT, and total bilirubin were predictors of longer post-radiation survival time (P＜0.05).CONCLUSION: The CT and clinical and laboratory assessment provide a reference for, and enable estimation of, probable survival times in HCC patients after radiotherapy. Tumor volume, severity of liver cirrhosis,status with respect to portal vein thrombosis and ascites and AFT, GOT/GPT and total bilirubin values were significant predictors of survival in this study.

关键词：肝细胞癌 CT检查血管栓塞反射线疗法