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Comparison of probiotics and lactulose in the treatment of minimal hepatic encephalopathy in rats

World Journal of Gastroenterology 2005年第6期11卷 908-911页

作者： LinJia Mei-HuaZhang departmentofdigestivediseases AffiliatedFirstPeople'sMunicipalHospitalGuangzhouMedicalCollegeGuangzhou510180GuangdongProvinceChina departmentofdigestivediseases AffiliatedFirstPeople'sMunicipalHospitalGuangzhouMedicalCollegeGuangzhou510180GuangdongProvinceChina

AIM: To compare the efficacy of probiotic preparation Golden Bifid and lactulose on rat experimental model of minimal hepatic encephalopathy (MHE) induced by thioactamide (TAA).METHODS: MHE was induced by intraperitoneal injection of TAA (200 mg/kg) every 24 h for two consecutive ***-six male MHE models were then randomly divided into 3 groups: TAA group (n = 12) received tap water ad libitum only; lactulose group (n = 12) and probiotics group (n = 12) were gavaged, respectively with 8 mL/kg of lactulose and 1.5 g/kg of probiotic preparation Golden Bifid (highly concentrated combination of probiotic)dissolved in 2 mL of normal saline, once a day for 8 d (from the 5th d before the experiment to the 3rd d of the experiment). The latency of brainstem auditory evoked potentials (BAEP) I was used as an objective index of MHE. The incidence of MHE, the level of serum endotoxin,ammonia, liver function and histological grade of hepatic injury of rats were examined ***: There were no overt HE and rat deaths in 3groups. The incidence of MHE, the levels of blood ammonia and endotoxin in TAA group, which were 83.3% (10/12),168.33±15.44 mg/dL and 0.36±0.04 EU/mL, respectively,were significantly higher than those in lactulose group,which were 33.3% (4/12), 110.25±7.39 mg/dL and 0.19±0.02 EU/mL, and probiotics group, which were 33.3% (4/12), 108.58±10.24 mg/dL and 0.13±0.03 EU/mL respectively (P ＜0.001). It showed that either probiotics or lactulose could significantly lower the level of hyperammonemia and hyper-endotoxemia, lighten centrolobular necrotic areas as well as inflammatory reaction in the liver of rats, normalize the latency of BAEP,and decrease the incidence of MHE. However, no significant differences were observed between these two groups (P ＞0.05).CONCLUSION: Probiotic compound Golden Bifid is at least as useful as lactulose for the prevention and treatment of MHE. Probiotic therapy may be a safe, natural, well-tolerated therapy appropriate

关键词： Minimal hepatic encephalopathy Probiotics Lactulose

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Antiangiogenic therapy for human pancreatic carcinoma xenografts in nude mice

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World Journal of Gastroenterology 2005年第3期11卷 447-450页

作者： LinJia Mei-HuaZhang Shi-ZhenYuan Wen-GeHuang departmentofdigestivediseases FirstAffiliatedPeople'sHospitalGuangzhouMedicalCollegeGuangzhou510180GuangdongProvinceChina departmentofdigestivediseases SecondAffiliatedHospitalSunYat-SenUniversityGuangzhou510120GuangdongProvinceChina Animalcenter SunYat-SenUniversityGuangzhou510089GuangdongProvinceChina departmentofdigestivediseases FirstAffiliatedPeople'sHospitalGuangzhouMedicalCollegeGuangzhou510180GuangdongProvinceChina

AIM: To investigate the anti-tumor effects of antiangiogenic therapy (a combination of TNP-470, an antiangiogenic compound, with gemcitabine, an antimetabolite) on human pancreatic carcinoma xenografts and its mechanism. METHODS: A surgical orthotopic implantation (SOI) model was established by suturing small pieces of SW1990 pancreatic carcinoma into the tail of pancreas in nude male mice. Mice then received either single therapy (n = 24) or combined therapy (n = 32). Mice receiving single therapy were randomly divided into control group, G100 group receiving 100 mg/kg gemcitabine IP on d O, 3, 6 and 9 after transplantation, and T30 group receiving 30 mg/kg TNP-470 s.c on alternate days for 8 wk. Mice receiving combined therapy were randomly divided into control group, T15 group, G50 group and combination group (TNP-470 30 mg/kg and gemcitabine 50 mg/kg). Animals were killed 8 wk after transplantation. Transplanted tumors, liver, lymph node and peritoneum were removed. Weight of transplanted tumors, the T/C rate (the rate of mean treated tumor weight to mean control tumor weight), change of body weight, metastasis rate, and 9-wk survival rate were investigated. Tumor samples were taken from the control group, T30 group, G100 group and combination group. PCNA index (PI) and microvessel density (MVD) were investigated by immunohistochemical staining for PCNA and factor VIII, respectively. RESULTS: There was a significant inhibitory effect on primary tumor growth of pancreatic carcinoma in G100 group, compared to T30 group, whereas tumor metastasis was significantly inhibited in T30 group compared to G100 group. There was no significant improvement in survival rate in these two groups. No significant inhibitory effect on tumor growth and metastasis in T15 group and G50 group. However, significant anti-tumor and anti-metastatic effects were observed in the combination group with a significant improvement in survival rate. The inhibitory effect on tumor growth in combin

关键词： Pancreatic carcinoma TNP-470 Angiogenesis Inhibitors Xenografts

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Treatment of severe Clonorchiasis sinensis by endoscopic nasobiliary drainage and oral praziquantel

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World Journal of Gastroenterology 2004年第14期10卷 2150-2152页

作者： Fa-ChaoZhi Xu-MingLiu Ze-QuanLiu YanLin Shu-JianChen PLAInstituteforDigestiveMedicine NanfangHospitalFirstMilitaryMedicalUniversityGuangzhou510515Guanl~donl~ProvinceChina departmentofdigestivediseases HexianHospitalPanyuGuangzhou511400GuangdongProvinceChina

AIM: To assess the therapeutic value of endoscopic nasobiliary drainage (ENBD) and oral praziquantel for severe Clonorchiasis sinensis ***: Of the 84 Clonorchiasis sinensis-infected patients enrolled, 58 were treated with ENBD (as observing group, ENBD group), 26 received operations (control group,operation group). Both of the two groups were comparable in terms of patient's age, body mass index. Before and one week after treatment, the average diameters of common bile ducts were measured by ultrasound, andserum bilirubin, ALP,γ-GT and ALT were detected by biochemical methods, After ENBD or operation, the patients took praziquantel for two days. RESULTS: Compared with the patients in operation group,ENBD patients in ENBD group had higher recovery rates of abdominal pain and fever as well as jaundice, quicker remission,smaller trauma, fewer complications and lower ***: ENBD combined with oral praziquantel is an effective and safe method for the treatment of severe Clonorchiasis sinensis.

关键词：治疗华支睾吸虫病内窥镜排脓法吡喹酮口服药

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Dynamic changes in the expression of matrix metalloproteinases and their inhibitors,TIMPs,during hepatic fibrosis induced by alcohol in rats

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World Journal of Gastroenterology 2004年第24期10卷 3621-3627页

作者： Guang-FuXu Peng-TaoLi Xin-YueWang XuJia De-LuTian Liang-DuoJiang Jin-XiangYang DepartmentofinfectiousDiseases 1^stAffiliatedDongzhimenHospitalBeijingUniversityofTraditionalChineseMedicineBeijing100700China departmentofdigestivediseases 1^stAffiliatedDongzhimenHospitalBeijingUniversityofTraditionalChineseMedicineBeijing100700China BasicMedicineCollegeofBeijingUniversityofTraditionalChineseMedicine Beijing100029China

AIM: To determine the dynamic changes in the expression of matrix metalloproteinases (MMPs) and the endogenous tissue inhibitors of MMPs inhibitors (TIMPs) during hepatic fibrosis induced by ***: Male Sprague-Dawley rats were randomly divided into normal, 4 d, 2 wk, 4 wk, 9 wk and 11 wk groups, and the model rats were fed with a mixture of alcohol by gastric infusion at the designed time, respectively, then decollated and their livers were harvested for the examination of MMP-2, MMP-3, MMP-9, MMP-13, TIMP-1 and TIMP-2 by immunohistochemistry, zymograghy and Western blotting, ***: Normal rats had moderate expression of MMP-2,which was decreased in the model rats except in the 11 wk group, where MMP-2 expression slightly increased. MMP-3 had the similar changing pattern to MMP-2 despite weaker expression. MMP-9 expression decreased in the 4 d and 2 wk groups, rose in the 4 wk group, decreased again in the 9 wk group and returned to normal levels in the 11 wk ***-13 expression decreased in the 4 d and 2 wk groups,and returned to normal levels in the 4 wk, 9 wk and 11 wk groups. TIMP-1 expression decreased in the 4 d and 2 wk groups, but sharply increased in the 4 wk group and sustained at a high level even after modeling was stopped for 2 wk. In normal rats TIMP-2 expression was strong. However, it decreased as soon as modeling began, and then gradually rose, but remained to a level lower than that in normal rats even after modeling was stopped for 2 ***: MMP-2 may not always expresses at a high level during hepatic fibrosis. MMP-13 and MMP-3 are acutely affected by TIMP-1. In this model TIMP-1 is the most powerful factor imposed on capillarization and peri-sinusoidal fibrosis. TIMP-2 is the most effective regulator on the metabolism of type Ⅳ collagen located in the basement of sinus.

关键词：动力变化基因表达矩阵金属蛋白抑制剂 TIMPs 肝纤维化诱导作用酒精老鼠消化系统

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Growth,invasion,metastasis,differentiation,angiogenesis and apoptosis of gastric cancer regulated by expression of PTEN encoding products

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World Journal of Gastroenterology 2003年第8期9卷 1662-1666页

作者： Wei-GuoJiang Yin-ChangZhang YanXin Hua-ChuanZheng Yi-LingLi Jin-MinSun Xue-FeiYang Xiao-HanLi DeparmaentofPathology TheSecondAffiliatedHospitalofChinaMedicalUniversityShenyang110004LiaoningProvinceChina CancerInstitute.TheFirstAffiliatedHospitalofChinaMedicalUniversity Shenyang110001LiaoningProvinceChina departmentofdigestivediseases TheFirstAffiliatedHospit~ofChinaMedicalUniversityShenyang110001LiaoningProvinceChina

AIM: To investigate expression of PTEN in gastric cancer and to explore its roles in tumorigenesis and progression of gastric ***: Formalin-fixed and paraffin-embedded tissues of adjacent non-tumor mucosa and primary foci from 113cases of gastric cancers were studied for the expression of PTEN and Caspase-3 andmicrovessel density (MVD)by streptavidin-peroxidase (S-P) immunohistochemistry with antibodies against PTEN, Caspase-3, and CD34. The relationship between PTEN and Caspase 3 expression and clinicopathological parameters of tumors was ***: Primary gastric cancer cells expressed PTEN less frequently than adjacent epithelial cells of primary foci (54.9％ vs89.4％; P=0.000, χ2=33.474). PTEN expression was significantly associated with invasive depth (P=0.003,rs=0.274), metastasis (P=0.036, rs=0.197), growth pattern (P=0.008, rs=0.282), Lauren′s classification (P=0.000,rs=0.345), and histological classification (P=0.005, rs=0.262)of tumors, but not with tumor size (P=0.639, rs=0.045),Borrmann′s classification (P=0.544, rs=0.070) or TNM staging (P=0.172, rs=0.129). PTEN expression was negatively correlated with MDV in primary gastric cancer (P=0.020,F=5.558). Primary gastric cancer cells showed less frequent immunoreactivity to Caspase-3 than adjacent epithelial cells of primary foci (32.7 ％ vs 50.4 ％; P=0.007,χ2=7.286).Caspase-3 expression was dependent of PTEN expression in primary gastric cancer cells (P=0.000, χ2=15.266).CONCLUSION: Down-regulated expression of PTEN plays an important role in tumorigenesis, progression, growth,differentiation and angiogenesis of gastric cancer. Low expression of PTEN can decrease expression of Caspase-3to disorder apoptosis of tumor cells, which might explain the molecular mechanisms of PTEN contributions to tumorigenesis and progression of gastric cancer.

关键词：胃癌 PTEN 基因表达肿瘤发生肿瘤侵袭肿瘤转移

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Effect of Hewei-Decoction on chronic atrophic gastritis and eradication of Helicobacter pylori

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World Journal of Gastroenterology 2005年第7期11卷 986-989页

作者： Wan-ShengJi Zhi-XingGao Kai-ChunWu Jun-WenQiu Bing-LongShi Dai-MingFan DepartmentofInternalMedicine WeifangMedicalCollegeWeifang261042ShandongProvinceChina InstituteofDigestiveDiseases XijingHospitalFourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina departmentofdigestivediseases IntegralHospitalofGuangzhouMilitaryCommandGuangzhou510000GuangdongProvinceChina

AIM: To demonstrate the effect of Hewei-Decoction (Decoction for regulating the stomach) on chronic atrophic gastritis (CAG) and eradication of Helicobacter pylori. METHODS: Ninety patients with CAG entering the investigation were divided into six differentiation syndromes, based on their major symptoms and signs. Hewei-Decoction was taken by all the patients orally for 4 or 8 wk. The efficacy was assessed by both the composite accumulation of reduced scores of major symptoms and the eradication of H pylori.X2 test was used to compare the efficacy between H pylori-positive and negative cases, and to disclose the relationship between efficacy and eradication of H pylori. RESULTS: In patients with six different syndrome types, the efficacy of Hewei-Decoction was 91.67% (11/12), 92.86% (13/14), 97.22% (35/36), 87.50% (14/16), 75.00% (6/8), 75.00% (3/4) respectively. The rate of highly efficacious was 58.33% (7/12), 50.00% (7/14), 77.78% (28/36), 62.50% (10/16), 12.50% (1/8) and 25.00% (1/4), respectively. The total efficacy was 91.11% (82/90), and the rate of highly efficacious was 60.00% (54/90). The eradication rate of H pylori was 67.86% (38/56). The therapeutic effect of Hewei-Decoction was better in H pylori positive cases than that in H pylori-negative cases with the total effect of 96.43% vs 82.35% (P<0.05). In 56 H pylori positive cases, the therapeutic effect was better in H pylori eradicated cases than that in H pylori-existent cases with the total effect of 97.37% vs 72.22% (P<0.01). CONCLUSION: Hewei-Decoction is effective in most cases of all the syndrome types. The results indicate that eradication of H pylori is one of the important mechanisms for alleviation of symptoms and signs. Also, the decoction is efficacious in H pylori-negative cases.

关键词： Chronic atrophic gastritis Helicobacter pylori infection Hewei-Decoction

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Apoptosis-inducing effect of recombinant Caspase-3 expressed by constructed eukaryotic vector on gastric cancer cell line SGC7901

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World Journal of Gastroenterology 2003年第9期9卷 1935-1939页

作者： Yuan-GenFu Yao-JunQu Kai-ChunWu Hui-HongZhai Zhi-GuoLiu Dai-MingFan DepartmentofBiochemistryandMolecularBiology MedicalCollegeShantouUniversityShantou515031.GuangdongProvinceChina DepartmentofDigestiveDisease LonggangCenterHospitalShenzhen16GuangdongProvinceChina InstituteofDigestiveDiseases FourthMilitaryMedicalUniversityXi'an710032ShaanxiProvinceChina departmentofdigestivediseases TheFirstHospitalNingxiaMetdicalCollegeYmchuan759901NingxiaHuiAutonmousRegionChina

AIM: To investigate the apoptosis-inducing effect of Caspases-3 expressed by constructed eukaryotic vector on gastric cancer cell line ***: PCR was employed to amplify the sequences of both small and large subunits of Caspases-3. Its products were separately cloned into the Sma I site of pBluescript KS+ to generate both plasmids pBS/SS and pBS/LS. The small subunit fragment was excised from plasmid pBS/SS with BarrH- I and then inserted into the BarnH I site of plasmid pBS/LS preceding that of the large subunit to yield plasmid pBS/Rev-Caspase-3. Rev-Caspase-3 cDNA was excised with Kpn Ⅰ+Xba I and then subcloned into plasmid pcDNA3.1(+) to construct Rev-Caspase-3 eukaryotic expression vector pcDNA/Rev-Caspase-3, which was used to transiently transfect SGC7901 cell line. Cell count, MTT assay and electron microscopy were used to confirm the antiproliferation and apoptosis-inducing effect of Rev-Caspase-3 expression on gastric cancer ***: Plasmid pBS/Rev-Caspase-3 and eukaryotic expression vector pcDNA/Rev-Caspase-3 were successfully constructed. SGC7901 cells were transiently transfected by either pcDNA/Rev-Caspase-3 or pcDNA3.1 (+) for 24, 48,72, and 96 h respectively. Cell growth was measured by cell count and MTT assay. In cell count assay, the cell numbers were 1.S×106, 1.55×106, 2.0×106, and 3.1×106 in the experimental group and 2.5×106, 3.1×106, 4.0×106, and 5.7×106 in the control group at 24, 48, 72 and 96 h *** growth of SGC7901 cells was suppressed by RevCaspase-3 in a time-dependent manner (P＜0.05). The results of MTT assay were similar to that of cell count (P＜0.05).The characteristics of apoptosis such as chromatin condensation, crescent formation and margination were seen and more obvious with time in the given-experimental period in the experimental group, but not easily observed in the control ***: The expression of Rev-Caspase-3 by the constructed eukaryotic vector can significantly induce apoptosis of g

关键词： Caspase-3 基因表达胃癌 SGC7901细胞细胞凋亡

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Spiral CT in gastric carcinoma:Comparison with barium study,fiberoptic gastroscopy and histopathology

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World Journal of Gastroenterology 2003年第7期9卷 1404-1408页

作者： FengChen Yi-ChengNi Kai-ErZheng Sheng-HongJu JunSun Xi-Long Man-HuaXu HaoZhang GuyMarchal DepartmentofRadiology ZhongdaHospital87DingjiaqiaoRoadNanjing210009JiangsuProvinceChina DepartmentofRadiology UniversityHospitalHerestraat49B-3000LeuvenBelgium departmentofdigestivediseases ZhongdaHospital87DingjiaqiaoRoadNanjing210009JiangsuProvinceChina

AIM: To evaluate spiral computed tomography (CT) including virtual gastroscopy for diagnosis of gastric carcinoma in comparison with upper gastrointestinal series (UGI),fiberoptic gastroscopy (FG) and ***: Sixty patients with histologically proven gastric carcinoma (54 advanced and 6 early) were included in this study. The results of spiral CT were compared with those of UGI and FG. Two observers blindly evaluated images of spiral CT and UGI and video recording of FG with consensus in terms of diagnostic confidence with a five-point *** of lesion detection, Borrmann′s classification of spiral CT, UGI and FG, as well as the accuracy of TNM staging of spiral CT were determined by comparing them to surgical and histological ***: The lesion detection rate was 98 % (59/60),95 % (57/60) and 98 % (59/60) for spiral CT, UGI and FG,respectively. There were no statistical differences in the detection sensitivity among the three techniques (P＞0.05).For the sensitivity in Borrmann′s classification, spiral CT was higher than that of UGI (P=0.025) and similar to that of FG (P＞0.05). The accuracy of spiral CT in staging the gastric carcinoma was 76.7 %. Six cases of early gastric carcinoma were all detected by spiral CT as well as ***: Spiral CT is equivalent to UGI and FG in the detection of gastric carcinoma, and superior to UGI but similar to FG in the Borrmann′s classification of advanced gastric carcinoma. Spiral CT is more valuable than FG in the staging of gastric carcinoma.

关键词：胃癌螺旋CT检查钡餐纤维胃镜检查组织病理学检查

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