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Gene expression of fibrinolytic factors urokinase plasminogen activator and plasminogen activator inhibitor-1 in rabbit temporo-mandibular joint cartilage with disc displacement

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Chinese Medical Journal 2005年第12期118卷 1000-1006页

作者： ZHANJing GUZhi-yuan WULi-qun ZHANGYin-kai HUJi-an DepartmentsofOralandMaxillofacialSurgery departmentofdentistry HospitalofSirRunRunShawZhejiangUniversityHangzhou310006China DepartmentsofOralPathology

Background The urokinase plasminogen activator system is believed to play an important role in degradation of the extracellular matrix associated with cartilage and bone destruction; however its precise roles in temporomandibular disorders have not yet been clarified. The aims of this study were to investigate the gene expression of fibrinolytic factors urokinase plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) in the articular cartilage of rabbit temporomandibular joint (TMJ) with disc displacement (DD) and to probe the relationship between fibrinolytic activity and cartilage remodeling. Methods Disc displacement of right joints was performed in 36 of 78 rabbits under investigation. The animals were sacrificed at 4 days and 1, 2, 4, 8 and 12 weeks after surgery, respectively. The right joints of these animals were harvested and processed for the examination of mRNA expression of uPA and PAI-1 in articular cartilage using in situ hybridization techniques. Results The expression of uPA and PAI-1 was co-expressed weakly in the chondrocytes from transitive zone to hypertrophic zone and mineralized zone, while no hybridizing signals were shown in proliferative zone and superficial zone in control rabbits. The most striking was the up-regulation of uPA and PAI-1 mRNA in 4-day rabbits postoperatively at the onset of cartilage degeneration. The strongest hybridizing signals for uPA and PAI-1 were seen in 2-week rabbits postoperatively. After 2 weeks, the expression of uPA and PAI-1 began to decrease and reached nearly normal level at 12 weeks. Conclusions The expression of the uPA/PAI-1 system coincides with the pathological changes in condylar cartilage after DD. The uPA/PAI-1 system may be one of the essential mediators in articular cartilage remodeling.

关键词： urokinase plasminogen activator · plasminogen activator inhibitor 1 · temporomandibular joint · disc displacement · in situ hybridization

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Neocartilage formation from predifferentiated human adipose derived stem cells in vivo

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Acta Pharmacologica Sinica 2007年第5期28卷 663-671页

作者： Xiao-bing JIN~2,Yong-sheng SUN~2,Ke ZHANG~2,Jing WANG~3,Xiao-dong JU~2,Si-quan LOU~(2,4)~2Department of Orthopedics,Peking University,Third Hospital,Beijing 100083,China ~3Department of Dentistry,College of Stomatology,Fourth Military Medical University,Xi'an 710032,China DepartmentofOrthopedics PekingUniversityThirdHospitalBeijing100083China departmentofdentistry CollegeofStomatologyFourthMilitaryMedicalUniversityXi'an710032China

Aim:To examine the chondrogenic potential of human adipose derived stem cells(hASC) induced by human transforming growth factor beta2 (hTGF beta2) invitro,and to investigate if predifferentiated hASC can produce neocartilage ***:hASC were isolated from subcutaneous adipose tissue and cul-tured in pellets with the addition of hTGF *** differentiationwas assayed by RT-PCR,Western blotting,toluidine blue staining,and immuno-histochemistry staining for collagen type Ⅱ.For the in vivo study,intact inducedcell pellets or the released cells embedded in alginate gel with different concentra-tions were implanted subcutaneously in nude *** were harvested atdifferent time points and carried with histological and immunohistochemistry ex-amination to evaluate the cartilage ***:RT-PCR analysis revealedthat hASC produced aggrecan and collagen type Ⅱ after 7 d of induction andcontinued throughout the culture *** was also demonstrated by theWestern blot analysis,positive staining of toluidine blue,and immunohistochem-istry for collagen type Ⅱ.After reseeding in the monolayer,the cells isolated fromthe pellets displayed a polygonal morphology compared with the primary spindleshape,hASC were released from the induced cell pellets when embedded inalginate gel (implanted cell concentration=5×10～6/mL or higher).They producedneocartilage after 12 weeks in vivo culture;however,intact induced cell pelletsimplanted subcutaneously rapidly lost their differentiated ***:Chondrogenesis of hASC in vitro can be induced by combining pellet culture andhTGF beta2 *** hASC embedded in alginate gel have theability of producing neocartilage in vivo.

关键词： human adipose derived stem cell (hASC) human transforming growth factor beta2 (hTGF beta2) cartilage: tissue engineering alginate

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Effect of the Thickess on Color of Porcelain Laminate Veneer

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Journal of Nanjing Medical University 2003年第2期17卷 74-77页

作者：陆支越章非敏殷新民 departmentofdentistry BeijingHospltalBeijing100730 DepartmentofProsthodontics theAffiliatedStomatologyofNanjingMedicalUniversityNanjing210029P.R.China

Objective : To evaluate the color change of the two-layer porcelain laminate veneer after adding veneering porcelain of different thickness to the 0. 2 mm thick aluminum oxide. Methods : Ten aluminum oxide disks were randomized into 2 groups. Each of five disks was veneered with Vita Lumin Shade 54 porcelain of thickness 0/0.4 mm and 0. 6 mm respectively. The L'a'b* color notations of the substrate covered with nan-veneered disks and 2 thickness porcelain veneered disks were measured separately and the color differences (AE) were calculated. Results ?Statistically significant differences in color coordinates of dark substrates were recorded after the substrate was covered by an aluminum oxide disk and also after the addition of veneering porcelain to the aluminum oxide disks in group 1 (P=0. 000 2) and group 2(P=0. 007 5) and between 2 groups (P=0. 000 1). Conclusion -The ability of masking underlying discoloration was increased and shifted to gray when the thickness of veneering porcelain increased.

关键词： porcelain laminate veneer thickness color

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暖牙胶垂直挤压术治疗根管源性牙髓根尖周病的临床应用

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临床口腔医学杂志 2001年第Z1期17卷 12-14页

作者：刘剑王贤福 Donald C.YU 暨南大学医学院口腔系广东广州51632 中国医科大学口腔医学院辽宁沈阳110002 departmentofdentistry FacultyofMediuine&DentistyUniversityofAlbertaEdnontonAlbertaCanada

目的　总结了 3年多暖牙胶垂直压术治疗根管源性牙髓根尖周病的临床疗效及成败因素。方法　追综观察 495例术后 1～ 3年的临床症状 ,X光片变化 ,用史氏标准评定疗效。结果　成功率为 99 2 %。结论　此术对主、侧支根管都能进行充填 ,且... 详细信息

目的　总结了 3年多暖牙胶垂直压术治疗根管源性牙髓根尖周病的临床疗效及成败因素。方法　追综观察 495例术后 1～ 3年的临床症状 ,X光片变化 ,用史氏标准评定疗效。结果　成功率为 99 2 %。结论　此术对主、侧支根管都能进行充填 ,且不受牙位置及根管弯曲的限制。

关键词：根管充填,垂直压力牙胶类根管充填材料,暖的治疗应用

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