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Blocking effects of genistein on cell proliferation and possible mechanism in human gastric carcinoma

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World Journal of Gastroenterology 2005年第1期11卷 69-72页

作者： Hong-BinCui Xiao-LinNa Dan-FengSong YingLiu DepartmentofFoodNutritionandHygiene PublicHealthCollegeHarbinMedicalUniversityHarbin150001HeilongjiangProvinceChina DepartmentofBiotechnology HarbinBusinessUniversityHarbin150076HeilongjiangProvinceChina

AIM: To study the blocking effects of genistein on cell proliferation cycle in human gastric carcinoma cells (SGC-7901) and the possible mechanism.METHODS: MTT assay was applied in the detection of the inhibitory effects of genistein on cell proliferation. Flow cytometry was used to analyze the cell cycle distribution.Immunocytochemical technique and Western blotting were performed to detect the protein expression of cyclin D1,cyclin B1 and p21waf1/cip1.RESULTS: Genistein significantly inhibited the growth and proliferation of human gastric carcinoma cells (SGC-7901).Seven days after treatment with different concentrations of genistein (2.5, 5.0, 10.0, 20.0 μg/mL), the growth inhibitory rates were 11.2%, 28.8%, 55.3%, 84.7%respectively and cell cycles were arrested at the G(2)/Mphase. Genistein decreased cyclin D1 protein expression and enhanced cyclin B1 and p21waf/cip1 protein expression in a concentration-dependent manner.CONCLUSION: The growth and proliferation of SGC-7901 cells can be inhibited by genistein via blocking the cell cycle,with reduced expression of cyclin D1 and enhanced expression of cyclin B1 and p21waf/cip1 protein in the concentration range of 0-20 μg/mL.

关键词：分芽繁殖胃癌肿瘤 SGC-7901 染料木素细胞系统

Isolation and characterization of heterotrophic nitrifying bacteria in MBR

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Journal of Environmental Sciences 2005年第4期17卷 589-592页

作者： LINYan HEYi-liang KONGHai-nan LIUBin-bin LIYan INAMONYuhei SchoolofEnvironmentalScienceandEngineering ShanghaiJiaotongUniversityShanghai200240China. DepartmentofBiotechnology ShanghaiJiaotongUniversityShanghai200240China NationalInstituteforEnvironmentalStudies16-20nogawa.Tsukuba IbarakiJapan）

The study presented the method for isolating the heterotrophic nitrifiers and the characterization of heterotrophic nitrification. Continuous tests via a membrane bioreactor (MBR) were operated under the controlled conditions to proliferate the nitrifiers. Heterotrophic nitrifying bacteria were isolated from the system in which the efficiency of total nitrogen(TN) removal was up to 80%. Since no autotrophic ammonium and nitrite oxidizers could be detected by fluorescence in situ hybridization(FISH), oxidized-N production was unlikely to be catalyzed by autotrophic nitrifiers during the heterotrophic nitrifiers' isolation in this study. The batch test results indicate that the isolated heterotrophic bacteria were able to nitrify. After 3 weeks incubation, the efficiencies of the COD removal by the three isolated bacterial strains B1, B2, and B3 were 52 6%, 71 7%, and 77 7%, respectively. The efficiencies of the TN removal by B1, B2, and B3 were 35 6%, 61 2% and 68 7%, respectively.

关键词： membrane bioreactor(MBR) simultaneous nitrification and denitrification(SND) heterotrophic nitrification aerobic denitrification

Effect of Long-term Intake of Rare Earth in Drinking Water on Trace Elements in Brains of Mice

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Journal of Rare Earths 2002年第5期20卷 562-564页

作者：刘洁生申治国杨维东车军谢利民雷衡毅 DepartmentofBiotechnology JinanUniversityGuangzhou510632China ShenzhenCenterforDiseaseControlandPrevention Shenzhen518020China SchoolofEarthandEnvironmentScience ZhongshanUniversityGuangzhou510275China

The mice were fed with water dissolved different level Y 3+ (0, 23.25, 2325.00 mg·L -1 ) for seven months, and the levels of trace elements such as Cu, Zn, Mn, Fe, Mg, Ni and Co in brains of F1 generation mice were then determined by POEMS. The results show that, compared with control, significant decreases in levels of Fe and Ni, increase in level of Co are observed, which suggested that Y 3+ may have an effect on the metabolism and distribution of some trace elements in brain, and then lead to changes of physiological function.

关键词： rare earths trace element mouse

A case of a patient infected with a hepatitis C virus genotype 3a multidrug resistant variant in Pakistan

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贫困所致传染病（英文） 2018年第1期

Background: Approximately 10 million people in Pakistan are infected with the hepatitis C virus (HCV). Most patients develop chronic hepatitis, with rare cases of spontaneous clearance. However, little is known about multidrug resistant viral variants in Pakistan.Findings: This case study describes a 47-year-old male diagnosed with chronic HCV genotype 3a infection in 2003. After an initial diagnosis of viral infection, the patient remained treatment na?ve for 5 years. He received two therapy cycles of interferon (IFN) plus ribavirin (RBV) in 2007 and 2010, however, he was non-responsive to the therapy. The patient then received an additional two treatment cycles of pegylated IFN α-2b plus RBV (in 2011 and 2013); he was still non-responsive. In 2016, the patient underwent sofosbuvir plus RBV combination therapy, however, the sustained virological response was still not achieved. The host genetic factor was found to be heterozygous guanine and thymine (GT) and cytosine and thymine (CT) genotypes of rs8099917 and rs12979860 polymorphism of IL28B, respectively. Phylogenetic analysis suggests that the resistant variant belong to an out-group and may require triple therapy.Conclusions: This is the first case that reports on a HCV-infected individual who was a non-responder to multiple IFN therapies in Pakistan. Further studies are needed to understand multidrug-resistant HCV variants in the Pakistani population.

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酸变性木薯淀粉的制备及其在高水分含量低温热处理的结构研究

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木薯精细化工 2002年第1期9卷 22-25页

作者： NapapronAtichokudomchai PavineeChinachoti 等 DepartmentofBiotechnology FacultyofScienceMahidolUniversityThailand DepartmentofFoodScience UniversityofMassachusettsAmherstMA01003USA

在本实验中，我们以酸解法获得有较高结晶度和一定机械强度的木薯变性淀粉，还进一步研究了这些淀粉在高水分含量的情况下，经低温热处理形成的有序分子结构，木薯原淀粉酸解的第一阶段（水解12－96h)，直链淀粉水解速度快，相对结晶度... 详细信息

在本实验中，我们以酸解法获得有较高结晶度和一定机械强度的木薯变性淀粉，还进一步研究了这些淀粉在高水分含量的情况下，经低温热处理形成的有序分子结构，木薯原淀粉酸解的第一阶段（水解12－96h)，直链淀粉水解速度快，相对结晶度急剧增加，第二阶段（水解192－768h)，几乎所有的直链淀粉都不存在，结晶区部分在地开始降解，最不稳定的结晶区域不再存在。另外，非均性大的结晶区域彼此分开，最后导致吸热曲线变宽，糊化焓可视为双螺旋结构的熔化能力，焓的提高是因为直链淀粉－支链淀粉之间的相互作用，从而在退火过程中导致更多的双螺旋结构或共结晶。

关键词：酸变性木薯淀粉制备高水分含量低温热处理糊化焓分子结构

A new way for chemical degradation of plastic by natural volatile constituents of Ledum palustre

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Chinese Science Bulletin 2003年第16期48卷 1718-1721页

作者： XIUZhilong ZHUXiuling ZHANGDaijia YINJianzhong WANGDehe ANLijia DepartmentofBiotechnology Dalian116023China DepartmentofMacromolecularMaterial Dalian116023China DepartmentofChemicalIndustrialMechanics Dalian116023China DepartmentofMaterialEngineering DalianUniversityofTechnologyDalian116023China

A novel phenomenon of degradation ofpolypropylene plastic by volatile constituents of Ledum palustre is reported in this study. The characteristic ofdegradation is described by tensile strength, melting point,and scanning electron micrographs as well as opticalmicrographs. The chemical composition of volatile oil ofLedum palustre is analyzed by gas chrography and massspectrometry. The mechanism of degradation and problemsneeded to be solved are also discussed.

关键词：聚丙烯塑料降解作用挥发性要素电子显微图光谱测定法拉申力量熔点

The Role of Extracellular Ca^(2+) Influx,Intracellular Ca^(2+) Release and Calm odulin in MouseEgg Fertilization

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Journal of Northeast Agricultural University(English Edition) 1999年第2期6卷 152-157页

作者： SunQing－yuan TanJing－he 等 StateKeyLaboratoryofReproductiveBiology instituteofZoologyAcademiaSinicabeijing100080PRC DepartmentofBiotechnology NortheastAgriculturalUniversityHarbin150030

The effects of various Ca 2+ modifying drugs on moue egg fertilization were studied.Ca 2+ chelator,ethylen glycol bis (2 aminoethyl) tetracetic acid(EGTA),and calmodulin (CaM) antagonist,trifluoperzaine(TFP),inhibited fertilization in a dose dependent manner,whild Ca 2+ channel bolcker,verapamil,did not have any effect.When intracellular Ca 2+ release was blocked by 8 (N,N diethylamino) octy1 3,4, 5 trimethoxybenzonate(TMB 8) or the Ca 2+ oscillations were inhibited by an inhibitor of endoplasmic reticulum Ca 2+ ATPase,thapsigargin,the second polar body emission and pronuclear formation were significantly decreased.In contrast,inhibition of intracellular Ca 2+ release via bolckage of inositol 1,4,5 triphosphate (IP3) production by neomycin or lithium did not affect fertilization.The results sugest that both extracellular influx,intracellular Ca 2+ release and CaM activation are required for normal fertilization.However,extracellular influx through voltage gated Ca 2+ channel and intracellular release induced by IP3 are not the only pathways for producing Ca 2+ transients in moue eggs.

关键词： calcium calmodulin fertilization mouse

Molecular Cloning and Prokaryotic Expression of Non-Structural Protein NS1 Gene of Porcine Parvovirus

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Agricultural Sciences in China 2003年第10期2卷 1175-1178页

作者： WUDan TONGGuang-zhi QIUHua-ji XUEQiang ZHOUYan-jun LIJing-peng NationalKeyLaboratoryofVeterinaryBiotechnology HarbinVeterinaryResearchInstituteHarbin150001P.R.China DepartmentofBiotechnology NortheastAgriculturalUniversityHarbin150030P.R.China

Porcine parvovirus (PPV) is one of the major agents causing swine reproductive failure. NS1 protein is a non-structural protein of PPV and can be used as a reagent for differentiation of vaccinated animals and infected ones. In present study, a recombinant plasmid pET28a/NS1 was constructed by cloning the coding sequence for NS1 of PPV into pET28a, a bacterial expression vector. The NS1 protein was expressed in E. coli BL21(DE3) after induced by IPTG and the recombinant fusion protein was purified with affinity chromatography. Expression amount of NS1 protein was improved by optimizing the inducing parameters. The recombinant NS1 protein is reactive to PPV positive sera in Western blot and ELISA test and therefore can be applicable in differential diagnosis of PPV infections.

关键词： Porcine parvovirus NS1 Diagnosis

Effect of Microwave and He-Ne Laser on Enzyme Activity and Biophoton Emission of Isatis indigotica Fort

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Journal of Integrative Plant Biology 2005年第7期47卷 849-855页

作者： Yi-PingCHEN Yong-JunLIU Xun-LingWANG Zhao-YuREN MingYUE CollegeofEnvironmentSciences Xi＇anUniversityofArchitectureandTechnologyXi＇an710048China CollegeofLifeSciences NorthwestUniversityXi＇an710069China InstituteofPhotonicsandPhoto-Technology NorthwestUniversityXi＇an710069China DepartmentofBiotechnology Xi＇anUniversityofEngineeringScienceandTechnologyXi＇an710048China／／InstituteofEarthEnvironmenttheChineseAcademyofSciencesXi＇an710075China

Abstract: The seed embryos of Isatis indigotica Fort were exposed to He-Ne laser (5.23 mW/mm2, radiated for 5 min) and microwave (1.26 mW/mm2, radiated for 8 s) irradiation to determine the effects of microwave and He-Ne laser pretreatment on enzyme activities, and biophoton emission of cotyledon. Then: (i) changes in the activities of enzymes in I. indigotica cotyledon (such as amylase, transaminase, and proteinase) were measured to investigate the effects of He-Ne laser and microwave pretreatment; and (ii) biophoton emission was measured to determine the speed of cell division and metabolism. Results from these experiments indicated that: (i) the activities of amylase, transaminase, and proteinase of the cotyledon pretreated by He-Ne laser and microwave were significantly increased; and (ii) the intensity of biophoton emission was enhanced significantly by He-Ne laser and microwave irradiation. These changes suggest that He-Ne laser and microwave pretreatment can improve the inner energy of seeds, lead to an enhancement of cotyledon enzymes, and speed up the metabolism of the cell, resulting in significantly increased biophoton emission. Moreover, the mechanism of action of the effects of laser and microwave radiation on the microcalorimetric parameters, enzyme activities, and biophoton emission of seeds is discussed on the basis of the results obtained.

关键词： biophoton emission enzymes activities Isatis indigotica laser microcalorimetric effect microwave