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Relationship between bicarbonate and cyclic nucleotide in the promoting effects on head—to—head agglutination in boar spermatozoa

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Asian Journal of Andrology 2002年第2期4卷 87-96页

作者： HiroshiHarayama SeishiroKato DepartmentofLifeScience GraduateSchoolofScienceandTechnology departmentofanimalscience FacultyofAgricultureKobeUniversityKobe657－8501japan

Aim: To clarify the relationship between bicarbonate and cAMP in the promoting effects on the sperm agglutination. Methods: Spermatozoa were collected from mature boars, washed and resuspended in a modified Krebs-Ringer HEPES lacking calcium chloride (mKRH). The sperm suspensions were incubated in a water bath (38.5 ) for 60 min and then the percentage of head-to-head agglutinated spermatozoa was determined. Results: Supplementation of the mKRH with sodium bicarbonate (5-10 mM) significantly raised the percentage of head-to-head agglutinated spermatozoa in the samples. The addition of selective inhibitors for calcium/calmodulin-dependent phosphodiesterases (type 1: 8-methoxymethyl-IBMX and vinpocetine, 25-50 mM) or for cAMP-specific phosphodiesterases (type 4: Ro20-1724 and rolipram, 25-50 mM) enhanced the effect of bicarbonate on sperm agglutination as highly as did the addition of non-selective inhibitors for phosphodiesterases (IBMX and papaverine, 25-50 mM). A calmodulin antagonist (W-7, 2 mM), that potentially blocks the stimulator of the calcium/calmodulin-dependent phosphodiesterases, significantly enhanced the effect of bicarbonate on sperm agglutination. Moreover, a phosphodiesterase-resistant cAMP analogue (cBiMPS, 0.1 mM) markedly induced agglutination in more spermatozoa (76%) after the incubation without bicarbonate and phosphodiesterase inhibitors than did a less potent cAMP analogue (dibutyryl cAMP, 1 mM) (21%), while three kinds of cGMP analogues (0.1-1 mM) had no effect on sperm agglutination. In addition, a cAMP antagonist (Rp-cAMPS, 1 mM) significantly reduced the sperm agglutination resulting from the actions of bicarbonate and IBMX. On the other hand, the effect of bicarbonate was abolished by a change of incubation temperature from 38.5 to 25. Conclusion: These findings demonstrate that the bicarbonate-induced agglutination of boar spermatozoa is controlled via the cAMP-mediated, temperature-dependent signaling cascade. This cascade is suppress

关键词： cAMP capacitation phosphodiesterase calmodulin

Study on pig growth hormone gene polymorphisms in western meat-type breeds and Chinese local breeds

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Journal of Zhejiang University Science 2003年第6期4卷 734-739页

作者：宋成义高波经荣斌陶勇毛九德 CollegeofAnimalScienceandVeterinaryMedicine YangzhouUniversityYangzhou225009China departmentofanimalscience UniversityofMissouri-ColumbiaColumbiaMO65211USA

Chinese Meishan and Jiangquhai pigs are two of the most prolific pigs in the world, but their growth rate is lower than that of Duroc, Landrace and Pietrain pigs. It is suggested that growth rate is regulated by growth hormone. The objective of the current study was to analyze the porcine growth hormone (p GH ) gene polymorphisms based on the polymerase chain reaction restriction fragment length polymorphism method (PCR RFLP) for three western meat type breeds (Duroc, Landrace and Pietrain) and two local Chinese pigs (Meishan and Jiangquhai). Five polymorphic restriction sites were detected with the Apa I, Msp I, Bsp I and Hha I restriction enzymes in two amplified fragments (605 bp, -119 to +486; 506 bp, +206 to +711). Breed difference was found only in the 506 bp fragment. There was no difference in allelic frequencies of Bsp I and Hha I restriction sites among the five breeds ( P >0.05). Landrace and Meishan pigs lacked allele G 3 of Msp I site. The allele G 3 frequency of restriction Msp I site of the 506 bp fragment in Pietrain pigs was higher than that in Duroc and Jianquhai pigs ( P 0.05 ). This new and rapid PCR RFLP typing method is an attractive tool for analysis of porcine growth hormone gene restriction sites. The differences in Msp I and Apa I restriction sites may explain the growth difference between the foreign meat type breeds above mentioned and local Chinese pigs.

关键词： Pigs Growth hormone gene Polymorphisms

First isolation and characterization of a new species of Vibrio from diseased flounder(Paralichthys olivaceus Temminck et Schlegel)

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Acta Oceanologica Sinica 2005年第3期24卷 131-140页

作者： ZHANGXiaojun FANGHai CHENCuizhen ZHANWenbin JINXiaomin WANGXiuyun departmentofanimalscience HebeiNormalUniversityofScienceandTechnologyQinhuangdao066600China LaboratoryofPathologyandImmunologyofAquaticAnimals KeyLaboratoryofMaricultureofMinistryofEducationOceanUniversityofChinaQingdao266003China

Appropriate pathogenic bacteria were isolated from diseased (or dead) flounder (Paralichthys olivaceus L) occurring in a mariculture farms of Qinhuangdao, which all diseased fishes expressed bacterial septicaemia. The phenotypic information of the 12 pure cultures was studied extensively, including morphological characteristics, colony characteristics in different media, physiological and biochemi- cal characteristics, and the mole fraction G+C ratio of the DNA for representative strain. The results show that the isolates belong to a new species of Vibrio, and are designated as Vibrio qinhuangdaora ***. by the area from which the diseased flounders are collected (Qinhuangdao) based on its biological properties following Rules of International Code of Nomenclature of Bacteria. At the same time, studies on the serum homology of isolates, and pathogenicity of isolates were conducted by experimental infection. The results show that all the isolates are of serologic similarity, and the isolates have strong pathogenicity to flounder. The studies can extensively reflect the main biological properties of this new species of Vibrio.

关键词： flounder (Paralichthys olivaceus L) Vibrio qinhuangdaora sp. nov. biological properties

The influence of cryoprotective media and processing procedures on motility and migration of frozen—thawed human sperm

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Asian Journal of Andrology 2002年第2期4卷 137-141页

作者： LindaS.McGonagle JosephFeldschuh 等 departmentofanimalscience CornellUniversityIthacaNY14853－4801USA IdantLaboratories 3505thAveNYNY10118USA

Aim: The study was designed to examine the effects of cryoprotective media, and glycerolating and thawing procedures on human sperm motility and gel penetrating ability. Methods: Fifteen unselected donors provided semen varying in quality that was distributed in a factorial design across three cryoprotectants (glycerol, egg yolk-citrate-glucose-glycerol and egg yolk-tris-glucose-glycerol). Also, glycerol was added at room temperature versus at 4. Two thaw temperatures were tested (laboratory air temperature for 10 min versus a 65 waterbath for 4 seconds). The proportion of total and progressively motile sperm was estimated immediately after thawing and following incubation at 35 for 2 h. Migration of sperm for 30 min at 37 through polyacrylamide gel was tested. Results: Donors differed greatly, with post-thaw total motility of sperm ranging from 9 to 44 % (P<0.05). Egg yolk-citrate-glucose-glycerol and egg yolk-tris-glucose-glycerol were superior to glycerol alone (post-thaw values of 35, 37 and 21 %, respectively, P<0.05). This was due primarily to poor sperm survival when semen was cooled to 4 without glycerol or egg yolk. The two thaw temperatures gave similar results. Sperm migration tests paralleled the motility results, but were more sensitive in detecting differences. Conclusion: Egg yolk, particularly in a tris-based medium that is widely used in domestic animals, improved the cryopreservation of both good and poor quality human semen.

关键词： Crypreservation Sperm migration Sperm motility Thaw temperature

Stable Skin-specific Overexpression of Human CTLA4-Ig in Transgenic Mice through Seven Generations

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Acta Biochimica et Biophysica Sinica 2006年第3期38卷 171-178页

作者： Yong WANG~1 Yong NI~(1,3) Hong WEI~1* Feng-Chao WANG~2 Liang-Peng GE~1 Xiang GAO~41 Department of Animal Science,College of Basic Medicine,Third Military Medical University,Chongqing 400038,China 2 Institute of Combined Injury,College of Military Preventive Medicine,Third Military Medical University,Chongqing 400038,China 3 School of Life Sciences,Southwest University,Chongqing 400715,China 4 Animal Model Research Institute,Nanjing University,Nanjing 210093,China departmentofanimalscience CollegeofBasicMedicineThirdMilitaryMedicalUniversityChongqing400038China InstituteofCombinedInjury CollegeofMilitaryPreventiveMedicineThirdMilitaryMedicalUniversityChongqing400038China

Skin graft rejection is a typical cellular immune response,mainly mediated by T *** lymphocyte associated antigen 4-immunoglobin (CTLA4-Ig) extends graft survival by blocking the T cellco-stimulation pathway and inhibiting T cell *** investigate the efficacy of CTLA4-Ig in prolong-ing skin graft survival,human CTLA4-Ig (hCTLA4-Ig) was engineered to overexpress in mouse skin bytransgenesis using the K14 *** transcription-polymerase chain reaction (RT-PCR) and West-ern blot assay indicated that the expression of CTLA4-Ig remained skin-specific and relatively constantcompared to the internal control protein,AKT,through seven *** presence and concentrationof the hCTLA4-Ig protein in transgenic mouse sera was determined by enzyme-linked immunosorbent assay(ELISA),and the results indicated that the serum CTLA4-Ig concentration also remained constant *** of transgenic mouse skins grafted onto rat wounds was remarkably prolonged com-pared to that of wild-type skins from the same mouse strain,and remained comparable among all *** suggested that the bioactive hCTLA4-Ig protein was stably expressed in transgenical micethrough at least seven generations,which was consistent with the stable skin-specific CTLA4-Ig *** results demonstrated that the transgenic expression of hCTLA4-Ig in skin driven by the K14 promoterremained constant through generations,and a transgenic line can be established to provide transgenic skinwith extended survival reproducibly.

关键词： cytotoxic T lymphocyte antigen 4-Ig transgenic mice skin K14 promoter

Growth Response and Fatty Acid Composition of Juvenile Procambarus clarkii Fed Different Sources of Dietary Lipid

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Agricultural Sciences in China 2003年第5期2卷 583-590页

作者： WENXiao-bo KUYao-mei ZHOUKai-ya departmentofanimalscience HubeiAgriculturalCollegeJingzhou434025P.R.China DepartmentofBiology NanjingNormalUniversityNanjing210097P.R.China

An experiment was conducted to evaluate the effects of feeding various sources of dietary lipid on weight gain, feed conversion, survival and fatty acid composition of juvenile red swamp crawfish, Procam-barus clarkii. Six semi-purified diets containing vitamin-free casein, defatted soybean meal, 0. 5% choline chloride, 0. 5% glycine and 0. 5% cholesterol were supplemented with 6. 0% of either anchovy oil, linseed oil, soybean oil, rapeseed oil, safflower oil or pork lard. Each diet was fed to crawfish (3. 07±0. 21 g average weight) in three replicate aquaria for 60 days. Survival rate, weight gain and feed conversion were best for crawfish fed the diet containing anchovy oil. Crawfish fed the linseed oil diet had the second highest weight gain, followed by crawfish on soybean oil, repaseed oil, safflower oil and pork lard diets, respectively. Feed conversion values were a reflection of weight gain. Results showed that both n-6 and n-3 fatty acids are dietary essential for juvenile Procambarus clarkii, although n-3 fatty acids promoted faster growth than n-6. However, highly unsaturated fatty acids (HUFA) (20 : 5n-3 and 22 :6n-3) had better growth-promoting effect than 18 : 3n-3, due probably to the limited ability of crawfish to bioconvert fatty acids to polyenoic forms of longer chain length. The fatty acid composition of the crawfish generally reflected that of the dietary lipids, especially for the diets containing unsaturated fatty acids.

关键词： Procambarus clarkii Fatty acid composition Feed conversion Highly unsaturated fatty acids Lipid sources Weight gain

Isolation and characterization of a new species of Vibrio-Vibrio olivaceus ***. from diseased flounder （Paralichthys olivaceus temminck et schlegel）

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High Technology Letters 2005年第1期11卷 107-112页

作者： FangHai ZhangXiao-jun ChenCuizhen ZhanWenbin JinXiaomin departmentofanimalscience HebeiNormalUniversityofScienceandTechnologyQinhuangdao066600P.R.China departmentofanimalscience HebeiNormalUniversityofScienceandTechnologyQinhuangdao066600P.R.China／LaboratoryofPathologyandImmunologyofAquaticAnimalsTheKeyLaboratoryofMaricultureMinistryofEducationOceanUniv LaboratoryofPathologyandImmunologyofAquaticAnimals TheKeyLaboratoryofMaricultureMinistryofEducationOceanUniversityofChinaQingdao266003P.R.China

One kind of pathogenic bacteria was isolated from diseased (or dead) flounder (Paralichthys olivaceus L.) which expressed bacterial septicaemia. The phenotyic information of the 10 pure cultures was studied extensively, including morphological characteristics, colony characteristics in different media, physiological and biochemical characteristics, and the mol%G+C ratio of the DNA for representative strain. The results showed that the isolates belonged to a new species of Vibrio. In addition, the representative strains have been re-checked by China Center for Type Culture Collection (CCTCC), the results were the same as ours, the examined strains were also regarded as a new species of Vibrio, and designated as Vibrio olivaceus ***. by its isolated fish (Paralichthys olivaceus) based on its biological properties following Rules of International Code of Nomenclature of Bacteria. In the same time, we conducted studies on the serum homology of isolates, pathogenicity of isolates by experimental infection. The results showed that all the isolates were of serologic similarity and the isolates have strong pathogenicity to flounder. Inspection to pure cultures and isolates from liver of moribund fish from infection experiment by fluorescent antibody technique showed the feasibility of fluorescent antibody technique as an auxiliary method of examination.

关键词：比目鱼鱼病生物特征弧菌微生物病原体

Effects of Source of Inorganic Phosphorus and Phytase Supplementation on Performance,Physiological Parameters and Bone Mineralization in Broilers

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Journal of Animal Science and Biotechnology 2010年第1期1卷 15-24页

作者： W.I.Lee P.A.Thacker Y.K.Han DepartmentofFoodScienceandBiotechnology SungkyunkwanUniversitySuwon440—746Korea departmentofanimalscience UniversityofSaskatchewan51CampusDriveSaskatoonSaskatchewanS7N5A8Canada

The objective of this study was to eval- uate the effects of inorganic phosphorus source and phytase addition on performance, nutrient digestibility and bone mineralization in broiler chickens. In Exp. 1,150 two-day old, male broiler chicks were fed a corn-soybean meal basal diet supplemented with phos- phorus provided by dicalcium phosphate, tricalcium phosphate or defluorinated rock phosphate. Five cages containing 10 birds were allotted to each of the three treatments. In Exp. 2,120 three-day old, male broiler chicks were fed the basal diet from Exp. 1 supplemen- ted with 0,250,500 ,or 1,000 P-＇rU phytase per kg of diet. Six cages containing five chicks were allotted to each of the four treatments. In Exp. 1, there was no difference in weight gain, feed intake or feed conver- sion as a result of feeding the different sources of in- organic phosphorus. The digestibility of phosphorus was significantly lower （P =0.01 ） for chicks fed di- ets supplemented with tricalcium phosphate than for chicks fed the other two diets. However, despite the lower digestibility, serum phosphorus levels did not differ among the three treatments. For Exp. 2, feedconversion showed a linear improvement （P = 0.03 ） with increasing levels of phytase inclusion （ days 0 to 33 ）. Phytase supplementation resulted in linear increa- ses in the digestibility of dry matter （P = 0.02 ）, crude protein （ P --- 0.04 ） and energy （ P 〈 0.01 ）. Chicks fed 1,000 FTU/kg phytase had significantly higher bone calcium （ P = 0.05 ） and bone breaking strength （P = 0.04 ） than chicks fed the basal diet on day 33. In conclusion, the results of the current study indicated that the performance of birds fed diets sup- plemented with dicalcium phosphate, tricalcium phos- phate or defluorinated phosphate was similar and therefore production costs could be lowered by choo- sing the cheapest inorganic phosphorus source when formulating diets for poultry. When diets were formu- lated to meet dietary phosphorus requirem

关键词： bone strength broilers digestibility inorganic phosphorus performance phytase

猪乳中一组高分子量蛋白的多态性及其与繁殖性能关系的研究

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畜牧兽医学报 2002年第5期33卷 429-432页

作者：秦宜德徐银学邹思湘 Walter L Hurley 南京农业大学农业部动物生理生化重点开放实验室南京210095 departmentofanimalscience

采用十二烷基硫酸钠聚丙烯酰胺凝胶不连续垂直板电泳 (SDS PAGE)对 16 2头二花脸母猪乳中一组高分子量蛋白质 (HMWP)进行了检测 ,计算了该位点的基因型频率、等位基因频率和遗传多样性指数等 ,并运用线性模型统计方法分析了该位点的... 详细信息

采用十二烷基硫酸钠聚丙烯酰胺凝胶不连续垂直板电泳 (SDS PAGE)对 16 2头二花脸母猪乳中一组高分子量蛋白质 (HMWP)进行了检测 ,计算了该位点的基因型频率、等位基因频率和遗传多样性指数等 ,并运用线性模型统计方法分析了该位点的不同基因型与母猪繁殖性能的关系。结果表明 ,在二花脸猪种群 ,乳中HMWP存在B(M :11480 0± 40 0 )和D(M :10 140 0± 6 0 0 )两种带 ,分别受二个等位基因HB 和HD 控制。BB、BD和DD三种基因型频率分别为 0 6 6 6 7、0 2 716和 0 0 6 17,两个等位基因HB 和HD 频率分别为 0 80 2 5和 0 1975 ,遗传多样性指数为0 3170。在三种HMWP基因型中 ,不同基因型母猪的繁殖性能在活仔数和窝重上存在着显著差异 (P 0 0 5 )。

关键词：猪乳高分子量蛋白质多态性繁殖性能遗传标记辅助选择