检索结果-南通市图书馆

Advances in Infectious Diseases 2024年第1期14卷 213-219页

作者： Janette Alejandra Gamiño Gutierrez Gustavo Adolfo Hernández Valdez Arleth Anahí Montaño Gómez Jorge Morales Rojas Graciela Gaddy Robles Martinez Anahi Herrera Ruelas Alin Kaori Ferreyra Gudiño Jocelin Celeste Jurado Cardona Jimena Jexabeth Ibáñez Núñez Anahí Fierros Ramos departmento de Neurocirugia Hospital Civil de Guadalajara Fray Antonio Alcalde Guadalajara México departamento de medicina Interna ISSSTE Tepic General Hospital APP Tepic México

Meningoencephalitis secondary to Listeria monocytogenes (L. monocytogenes) mainly affects newborns, the elderly and immunocompromised people;there are extremely rare cases in which said infection occurs in immunocompetent individuals. We present the case of a young adult patient without immunocompromise, who developed meningoencephalitis due to L. monocytogenes;This case is exceptional, since it occurred in an individual outside the classic age group, in addition to not having risk factors, which is why it should be considered an atypical causal agent.

关键词： Meningitis Encephalitis Rhombencephalitis Listeria monocytogenes Immunocompetent

mRNA levels of TLR4 and TLR5 are independent of H pylori

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第34期14卷 5306-5310页

作者： Elvira Garza-González Virgilio Bocanegra-García Francisco Javier Bosques-Padilla Juan Pablo Flores-Gutiérrez Francisco Moreno Guillermo Ignacio Perez-Perez departmento de Microbiología Facultad de MedicinaUniversidad Autónoma de Nuevo León departmento de Biología Molecular y Bioingeniería UAM Reynosa Aztlán Universidad Autónoma de Tamaulipas Hospital Universitario "Dr. José Eleuterio González" Universidad Autónoma de Nuevo León department of Medicine and Microbiology New York University School of Medicine

AIM:To determine if the presence H pylori or its viru- lence affect toll-like receptor 4 (TLR4) and TLR5 mRNA expression levels. METHODS:For the in vivo assays, gastric biopsies were obtained from 40 patients and H pylori status was determined. For the in vitro assays, human gastric adenocarcinoma mucosal cells (AGS) were cultured in the presence or absence of twelve selected H pylori strains. H pylori strains isolated from culture-positive patients and selected strains were genotyped for cagA and vacA. The cDNA was obtained from mRNA extracted from biopsies and from infected AGS cells. TLR4 and TLR5 mRNA levels were examined by real-time PCR. RESULTS: The presence of H pylori did not affect the mRNA levels of TLR4 or TLR5 in gastric biopsies. The mRNA levels of both receptors were not influenced by the vacA status (P > 0.05 for both receptors) andthere were no differences in TLR4 or TLR5 mRNA levels among the different clinical presentations/histological fi ndings (P > 0.05). In the in vitro assay, the mRNA levels of TLR4 or TLR5 in AGS cells were not influenced by the vacAs1 status or the clinical condition as-sociated with the strains (P > 0.05 for both TLR4 and TLR5). CONCLUSION: The results of this study show that the mRNA levels of TLR4 and TLR5 in gastric cells, both in vivo and in vitro, are independent of H pylori colonization and suggest that vacA may not be a significant player in the first step of innate immune recognition mediated by TLR4 or TLR5.

关键词： H pylori Toll-like receptor 4 Toll-likereceptor 5 AGS cells mRNA

Entamoeba histolytica acetyl-CoA synthetase:biomarker of acute amoebic liver abscess

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2014年第6期4卷 446-450页

作者： Lim Boon Huat Alfonso Olivos Garcia Tan Zi Ning Wong Weng Kin Rahmah Noordin Siti shafiqah Anaqi Azham Lee zhi Jie Guee Cher Ching Foo Phiaw Chong Pim Chau Dam Biomedicine Programme.School of Health Sciences Universiti Sains Malaysia departmento de medicina Experimental Facultad de MedicinaUniversidad Nacional Autonoma de Mexico department of Medicine Allianze University College of Medical Sciences Institute for Research in Molecular Medicine 11800 University Sains Malaysia

Objective:To characterize the Entamoeba histolytica(***)antigen(s)recognized by moribound amoebic liver abscess ***:Crude soluble antigen of *** was probed with sera of moribund hamsters in 1D-and 2D-Westem blot *** antigenic protein was then sent for tandem mass spectrometry *** corresponding gene was cloned and expressed in Escherichia coli BL21-AI to produce the recombinant *** ADP-forming acetyl-CoA synthetase(EhACS)protein.A customised ELISA was developed to evaluate the sensitivity and specificity of the recombinant ***:A^75 kDa protein band with a pl value of 5.91-6.5 was found to be antigenic;and not detected by sera of hamsters in the control *** mass spectrometry analysis revealed the protein to be the 77 kDa *** ADP-forming acetyl-CoA synthetase(EhACS).The customised ELISA results revealed 100%sensitivity and 100%specificity when tested against infected(n=31)and control group hamsters(n=5)serum samples,***:This rinding suggested the significant role of EhACS as a biomarker for moribund hamsters with acute amoebic liver abscess(ALA)*** is deemed pertinent that future studies explore the potential roles of EhACS in better understanding the pathogenesis of ALA;and in the development of vaccine and diagnostic tests to control ALA in human populations.

关键词： Entamoeba histolytica Amoebic liver abscess Biomarker Acetyl-CoA synthetase Recombinant EhACS Western blot

Circulating leptin and its muscle gene expression in Nellore cattle with divergent feed efficiency

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2018年第1期9卷 228-232页

作者： Lúcio Flávio Macedo Mota Cristina Moreira Bonafé Pamela Almeida Alexandre Miguel Henrique Santana Francisco JoséNovais Erika Toriyama Aldrin Vieira Pires Saulo da Luz Silva Paulo Roberto Leme JoséBento Sterman Ferraz Heidge Fukumasu departmento de Zootecnia Universidade Federal dos Vales do Jequitinhonha e MucuriDiamantinaMG 39100 000Brazil Faculdade de Ciencias Agrarias e Veterinarias Universidade Estadual Paulista JaboticabalSP 14884 900Brazil departamento de medicina Veterinaria Faculdade de Zootecnia e Encjenharia de AlimentosUniversidade de Sao PauloAM.Duque de Caxias Norte n225Pirassununcja 13635 900SPBrazil departmento de Zootecnia e desenvolvimento Acjrossocioambiental Sustentavel Faculdade de VeterinariaUniversidade Federal FluminenseNiteroiRJ 24230 340Brazil departamento de Zootecnia Faculdade de Zootecnia e Engenharia de AlimentosUniversidade de Sao PauloPirassununga 13635-900SPBrazil

Background： Leptin has a strong relation to important traits in animal production, such as carcass composition,feed intake, and reproduction. It is mainly produced by adipose cells and acts predominantly in the *** this study, circulating leptin and its gene expression in muscle were evaluated in two groups of young Nellore bulls with divergent feed efficiency. Individual dry matter intake（DMI） and average daily gain（ADG） of 98 Nellore bulls were evaluated in feedlot for 70 d to determinate the residual feed intake（RFI） and select 20 animals for the high feed efficient（LRFI） and 20 for the low feed efficient（HRFI） groups. Blood samples were collected on d 56 and at slaughter（80 d） to determine circulating plasma leptin. Samples of Longissimus dorsi were taken at slaughter for leptin gene expression ***： DMI and RFI were different between groups and LRFI animals showed less back fat and rump fat thickness,as well as less pelvic and kidney fat weight. Circulating leptin increased over time in all animals. Plasma leptin was greater in LRFI on 56 d and at slaughter（P = 0.0049）. Gene expression of leptin were greater in LRFI animals（P = 0.0022） in accordance with the plasma levels. The animals of the LRFI group were leaner, ate less, and had more circulating leptin and its gene ***： These findings demonstrated that leptin plays its physiological role in young Nellore bulls, probably controlling food intake because feed efficient animals have more leptin and lower residual feed intake.

关键词： Beef cattle Energy homeostasis Fat depositon Residual feed intake

Permanence of Lung Disorders after Hospital Discharge of Patients Who Underwent Cardiac Surgery: An Integrative Review

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Health 2020年第2期12卷 194-203页

作者： Daniela Di Bernardi Marcos Bagrichevsky Christiani decker Batista Bonin Instituto de Ensino Superior (IES) Florianópolis Brasil departmento de medicina Universidade Regional de Blumenau (FURB) Blumenau Brasil

Patients are more likely to develop lung disorders after cardiac surgery. Pulmonary dysfunction resulting from cardiac surgery in the hospital phase is well documented. However, there is a scarcity of studies related to the permanence of pulmonary disorders in the period following hospital discharge. The objective of this study was to verify in a database investigation the permanence of pulmonary dysfunctions after hospital discharge of patients who underwent cardiac surgery. A literature review with an integrative approach was carried out in the National Library of Medicine (Pubmed) and Scientific Electronic Library Online (Scielo) databases from 1978 to 2019. 4523 articles were found and after applying the inclusion and exclusion criteria, the sample was composed of 14 articles relevant to the subject and that were available in full. The results demonstrated the presence of atelectasis, pleural effusion, diaphragmatic dysfunction, respiratory symptoms such as cough, phlegm, wheezing, and dyspnea, reduced static and dynamic lung volumes, reduced diffusion capacity and respiratory muscle strength, in the period that ranged from three weeks to one year after cardiac surgery. Further studies are needed to elucidate the mechanisms that lead to the permanence of pulmonary dysfunctions after hospital discharge in patients who underwent cardiac surgery.

关键词： Thoracic Surgery Cardiac Surgery Pulmonary Function Test Respiratory Function Tests

Viscoelastic Models for Passive Arterial Wall Dynamics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Applied Mathematics and Mechanics 2009年第2期1卷 151-165页

作者： D.Valdez-Jasso H.T.Banks M.A.Haider D.Bia Y.Zocalo R.L.Armentano M.S.Olufsen department of Mathematics and Center for Research in Scientific Computation NC State UniversityCampus Box 8205RaleighNC 27695 departmento de Fisiologia Facultad de MedicinaUniversidad de La RepublicaGeneral Flores 2125PC:11800MontevideoUruguay Facultad de Ingenieria y Ciencias Exactas y Naturales Universidad FavaloroAvda.Belgrano 1723Capital FederalBuenos AiresArgentina

This paper compares two models predicting elastic and viscoelastic properties of large *** compared include a Kelvin(standard linear)model and an extended 2-term exponential linear viscoelastic *** were validated against in-vitro data from the ovine thoracic descending aorta and the carotid *** of blood pressure data were used as an input to predict vessel cross-sectional *** properties were predicted by estimating a set of model parameters that minimize the difference between computed and measured values of the cross-sectional *** model comparison was carried out using generalized analysis of variance type statistical *** the thoracic descending aorta,results suggest that the extended 2-term exponential model does not improve the ability to predict the observed cross-sectional area data,while for the carotid artery the extended model does statistically provide an improved fit to the *** is in agreement with the fact that the aorta displays more complex nonlinear viscoelastic dynamics,while the stiffer carotid artery mainly displays simpler linear viscoelastic dynamics.

关键词： Arterial wall modeling dynamic viscoelastic models inverse problems statistical analysis for model comparison

Leptin Causes the Early Inhibition of Glycolysis-and TCA Cycle-Related Genes in the Brain of Ob/Ob Mice to Restore Fertility

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

American Journal of Molecular Biology 2014年第3期4卷 105-113页

作者： Carlos Fernandes Baptista Samuel Marcos Ribeiro de Noronha Maria de Nazareth Gamboa Ritto Eduardo Henrique da Silva Freitas Melquíades Pereira Júnior Mauro Abi Haidar Ismael Dale Cotrim Guerreiro da Silva Silvana Aparecida Alves Corrêa de Noronha Marisa Teresinha Patriarca Gynecology department Escola Paulista de Medicina/Universidade Federal de Sao Paulo(EPM-UNIFESP)São PauloBrazil departmento de Cirurgia Geral e Especializada Universidade Federal do Rio de Janeiro(UNIRIO)Rio de JaneiroBrazil Surgery department Escola Paulista de Medicina/Universidade Federal de Sao Paulo(EPM-UNIFESP)Sao PauloBrazil departmento de medicina Geral Universidade Federal do Rio de Janeiro(UNIRIO)Rio de JaneiroBrazil

Introduction: Polycystic ovarian syndrome (PCOS) is undoubtedly the commonest androgen disorder in woman’s fertile period and certainly one of the most prevalent causes of anovulation. The syndrome has an estimated prevalence of 4% - 10% among women of childbearing age. Previously, our group demonstrated the effect of gonadal white adipose tissue transplantation from wild-type lean and fertile female mice to isogenic obese anovulatory ob/ob mice. These complex metabolic interrelationships between obesity and PCOS have yet to be fully understood. The aim of this study was to evaluate the effect of gonadal white adipose tissue (WAT) transplantation from the wild-type lean and fertile female mice to isogenic obese, anovulatory mice (Lep ob/Lep ob) on the expression of glycolysis- and TCA cycle-related genes and obtain a general view of the glucose metabolism in the brain of these animals. Methods: Fifteen ob/ob mice ranging from 2 to 3 months of age were divided into 3 experimental groups: control normal weight (n = 5), obese control (n = 5) and obese 7 days leptin treated (n = 5). The whole brains of the mice were processed for RNA extraction. The samples from each group were used to perform PCR assays using an array plate containing 84 primers to study the glucose metabolism-related genes. Results: The glycolysis- and TCA cycle-related genes were significantly downregulated. The most significantly affected genes were as follows: for glycolysis (fold regulation with p deno3 (119, 45, 18, and 28 times less, respectively);and for the TCA cycle (fold regulation with p decrease in the glucose metabolism. These results confirm the ability of the adipose tissue-derived hormone leptin to regulate early crucial genes that are related to glycolysis mechanisms and to the TCA cycle. This hormone seems to revert early the central phys

关键词： PCOS Obesity Leptin Glycolysis TCA Cycle Gene Expression

Non-Painful Peripheral Inflammation Blocks Conditioned Place-Preference to Morphine and Nicotine through an Ibuprofen-Sensitive and an Ibuprofen Insensitive Pathway

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Behavioral and Brain Science 2018年第2期8卷 57-76页

作者： Marco Antonio Sotomayor-Sobrino Abraham Ochoa-Aguilar Eda Patricia Tenorio Martha León-Olea Roberto Velasco-González Israel Luna-Mendoza Claudia Gómez-Acevedo departmento de Farmacología Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México México departmento de Bioquímica Facultad de Medicina Universidad Nacional Autónoma de México Ciudad de México México Instituto Nacional de Psiquiatría “Ramón de la Fuente Mu&ntilde iz” Ciudad de México México

The field of neuroimmunology has expanded in recent years providing new insights and therapies into pathologies like stroke, autism, and depression. However, few works explore the relationship between inflammatory stimuli and motivation. Thus, the aim of this study was to determine how non-painful inflammatory stimuli affect reward. To test reward-response, we used the morphine and the nicotine induced conditioned place-preference and place-aversion model in rats with non-painful inflammation. The following inflammatory models were used: non-painful infectious inflammation: 24 hrs prior to conditioning sessions, an injection with Calmette-Guerin bacillus (CGB) 1 × 107 cfu, ip, was administered. Non-painful non-infectious inflammation: 24 hrs prior to conditioning sessions, rats’ sciatic nerve was blocked and cut, followed by the injection of carrageenan (750 μl) in the paw. We then measured the cytokine concentration to determine the inflammatory profile of each of our models. Finally, we administered ibuprofen to determine if it could prevent the effect of inflammation over conditioned place-preference. We show that carrageenan significantly reduced the morphine-induced reward. Non-painful inflammatory stimulus, CGB and denervation + carrageenan, inhibit the conditioned place-preference to morphine and nicotine, CGB also block conditioned place-aversion to nicotine;carrageenan has no effect on CPA. The administration of ibuprofen reinstates conditioned place-preference to morphine and nicotine in the carrageenan model, but has no effect in the CGB model;finally ibuprofen has no effect on CPA. Our data suggest that non-painful-inflammatory stimuli inhibit the reward system, independent of cytokine concentration. Furthermore, the administration of a PGE 2 inhibitor can importantly modulate this phenomenon.

关键词： Reward Addiction Pain Inflammation Prostaglandins Interferon

Characteristics of Contraceptive Use in So Paulo State,Brazil

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Reproduction and Contraception 2006年第2期17卷 89-96页

作者： Elisabeth Meloni Vieira Rita Badiani Amaury Léis Dal Fabbro Antonio Luiz Rodrigues Junior departamento de medicina Social da Faculdade de medicina de Ribeiro Preto da Universidade de So Paulo Brazil departmento de Programs Pathfinder International Abuja Nigeria Hospital das Clínicas da Faculdade de medicina da Universidade de So Paulo So Paulo SP Brazil

Objective To know the characteristics of contraceptive use in Sao Paulo State collected by the demographic and Health Survey （DHS） conducted in 1996. Methods Data of Sao Paulo in 1996 were analyzed compared with the previous DHS carried on in 1986focusing on Sao Paulo State. Contraceptive use among married or cohabiting women by age, number of children and schooling was closely examined. Age and timing of female sterilization were also analyzed. Results The results showed that unlike Brazil there was a stability of female sterilization in Sao Paulo State during 1986 and 1996. There was a pattern in Sao Paulo： up to 30 years old the pill was the most used contraceptive, after 30 there was a predominance of female sterilization, which increased with the number of children and decreased with schooling. The use of male methods had also increased in the 1986 and 1996 period, being greater in Sao Paulo than in Brazil. Sao Paulo also presented more diversity in the use of reversible contraceptives. Conclusions The predominant two contraceptive methods in Brazil and Sao Paulo changed from 1986 to 1996, i.e..from pill to female sterilization.

关键词： contraceptive family planning contraceptive use female sterilization pill

Ventral Tegmental Area Neuronal Activity Correlates to Animals’ Behavioral Response to Chronic Methylphenidate Recorded from Adolescent SD Male Rats

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Behavioral and Brain Science 2014年第4期4卷 168-189页

作者： Zachary Jones Cruz Reyes Vazquez Nachum Dafny department of Neurobiology and Anatomy University of Texas Medical School at Houston Houston USA departmento de Fisiologia Divivion de Investigacion Facultad de Medicina Universidad Nacional Autonoma de Mexico Mexico City Mexico

Methylphenidate (MPD) is considered as the first-line pharmacotherapy to treat ADHD. More recently, MPD has also been used as a cognitive enhancement recreationally. Its therapeutic effects are not fully understood, nor are the long term effects of the drug on brain development. The ventral tegmental area (VTA) neuronal activity was recorded from freely behaving adolescent rats using a wireless recording system. Five groups were used: saline, 0.6, 2.5, 5.0 and 10.0 mg/kg MPD. The experiment lasted for 10 days. This study demonstrated that VTA neurons respond to MPD in a dose response characteristic and the same dose of MPD can cause both behavioral sensitization and behavioral tolerance. The neuronal unit activity was evaluated based on the animals’ behavioral activity following chronic MPD administration. The study showed that the animals’ behavioral response to different acute MPD of 0.6, 2.5 and 10.0 mg/kg doses responded in a dose response characteristics. Moreover, the same chronic dose of 0.6, 2.5, and 10.0 mg/kg MPD elicits in some animals’ behavioral sensitization and in some others behavioral tolerance. Therefore, the neuronal activity recorded from animals expressing behavioral sensitization was analyzed separately from the neuronal activity recorded from of behaviorally tolerant animals and it was found that the VTA units of the behaviorally sensitization animals responded significantly different to the drug than those VTA units recorded from animals expressing behavioral tolerance.

关键词： Ritalin Electrophysiology Neuronal Activity Behavior Ventral Tegmental Area