检索结果-南通市图书馆

LOWER DOSE OF AMINOGLYCOSIDE OTOTOXIC EXPOSURE CAUSES PRESYNAPTIC ALTERATIONS ASSOICATED WITH HEARING LOSS

Journal of Otology 2014年第1期9卷 36-42页

作者： LIU Ke WANG Xiaoyu LI Sijun TANG Siquan XU Yice WANG Xuefeng SUN Jianhe YANG Weiyan YANG Shiming department of otolaryngology-Head and Neck Surgery PLA General Hospital PLA Institute of Otolaryngology department of otolaryngology-Head and Neck Surgery the Affiliated Hospital North SiChuan Medical College department of otolaryngology-Head and Neck Surgery the First Affiliated Hospital Liaoning Medical University

Objective To study presynaptic alternations of cochlear ribbons arising from aminoglycoside ototoxic stimuli in C57BL/6J mice. Methods Animals were injected with low dose gentamicin (100 mg/kg/day) for 14 days, From the 14th to 28th days, the mice were maintained free of gentamicin treatment. Immunohisto-chemistry labeling was employed to trace RIBEYE, a major presynaptic componment of ribbon synapses. RIBEYE/CtBP2 expression levels were assessed and compared with hearing threshold shifts. Auditory func-tion was assessed by auditory brainstem responses. The stereocilia of outer hair cells (OHCs) and IHCs was examined by scanning electron microscopy (SEM). Results Hearing thresholds were elevated with peak hearing loss observed on the 7th day after gentamicin exposure, followed by improvement after the 7th day. RIBEYE/CtBP2 expression directly correlated with observed hearing threshold shifts. Strikingly, we did not see any obvious changes in stereocilia in both OHCs and IHCs until the 28th day. Mild changes in stereocil-ia were only observed in OHCs on the 28th day. Conclusions These findings indicate that presynapse co-chlear ribbons, rather than stereocilia, may be sensitive to aminoglycoside ototoxic exposure in mice cochle-ae. A pattern of RIBEYE/CtBP2 expression changes seems to parallel hearing threshold shifts and suggests presynaptic response properties to lower dosage of aminoglycoside ototoxic stimuli.

关键词： Inner Hair Cells Ribbon Synapse Aminoglycoside Ototoxicity RIBEYE/CtBP2 Stereocilia

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Translocation of telomerase reverse transcriptase coincided with ATP release in postnatal cochlear supporting cells

引用

Neural Regeneration Research 2024年第5期19卷 1119-1125页

作者： Yukai Zhang Keyong Tian Wei Wei Wenjuan Mi Fei Lu Zhenzhen Liu Qingwen Zhu Xinyu Zhang Panling Geng Jianhua Qiu Yongli Song Dingjun Zha department of otolaryngology Xijing HospitalFourth Military Medical UniversityXi’anShaanxi ProvinceChina

The spontaneous bursts of electrical activity in the developing auditory system are derived from the periodic release of adenosine triphosphate(ATP)by supporting cells in the Kölliker’s ***,the mechanisms responsible for initiating spontaneous ATP release have not been *** previous study revealed that telomerase reverse transcriptase(TERT)is expressed in the basilar membrane during the first postnatal *** role in cochlear development remains *** this study,we investigated the expression and role of TERT in postnatal cochlea supporting *** results revealed that in postnatal cochlear Kölliker’s organ supporting cells,TERT shifts from the nucleus into the cytoplasm over *** found that the TERT translocation tendency in postnatal cochlear supporting cells in vitro coincided with that observed in *** analysis showed that TERT in the cytoplasm was mainly located in mitochondria in the absence of oxidative stress or apoptosis,suggesting that TERT in mitochondria plays roles other than antioxidant or anti-apoptotic *** observed increased ATP synthesis,release and activation of purine signaling systems in supporting cells during the first 10 postnatal *** phenomenon that TERT translocation coincided with changes in ATP synthesis,release and activation of the purine signaling system in postnatal cochlear supporting cells suggested that TERT may be involved in regulating ATP release and activation of the purine signaling *** study provides a new research direction for exploring the spontaneous electrical activity of the cochlea during the early postnatal period.

关键词： apoptosis ATP release Ca2+transients cochlea mitochondrial function reactive oxygen species spontaneous electrical activity supporting cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

From nuclear submarines to graduate medical education: applying David Marquet's intent-based leadership model

引用

Military Medical Research 2018年第1期5卷 90-91页

作者： Camilo Fernandez-Salvador Rebecca Oney Sungjin A.Song Macario Camacho department of otolaryngology Tripler Army Medical Center otolaryngology-Head and Neck Surgery Tripler Army Medical Center

*** Marquet,a decorated Navy Captain,transformed an under performing submarine crew by empowering his subordinates to be leaders and reach their full *** called this intent-based leadership(IBL).What would happen if Marquet’s model were implemented in Graduate Medical Education(GME)?In this letter to the editor,we summarize the potential of the IBL model in graduate medical education as opposed to the traditional leaderfollower *** harnesses human productivity toward the shared goals of GME,which are patient care and trainee *** shift in mindset could lead both teachers and trainees to focus more on the real reason that we undertake GME and change behaviors for the *** suggest that IBL can and should be adopted in GME and propose that both patients and providers will benefit from this action.

关键词： David Marquet Leadership Graduate medical education Intent-based leadership

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

HSP90AA1 promotes lymphatic metastasis of hypopharyngeal squamous cell carcinoma by regulating epithelial-mesenchymal transition

引用

Oncology Research 2023年第5期31卷 787-803页

作者： FENGXIANG TANG YANSHI LI MIN PAN ZHIHAI WANG TAO LU CHUAN LIU XIN ZHOU GUOHUA HU department of otolaryngology The First Affiliated Hospital of Chongqing Medical UniversityChongqingChina

Lymphatic metastasis(LM)emerges as an independent prognostic marker for hypopharyngeal squamous cell carcinoma(HSPSCC),chiefly contributing to treatment *** study aimed to scrutinize the prognostic relevance of HSP90AA1 and its potential regulatory mechanism of concerning LM in ***:In a preceding investigation,HSP90AA1,a differential gene,was discovered through transcriptome sequencing of HPSCC tissues,considering both the presence and absence of *** of HSP90AA1 expression was accomplished via qRT-PCR,western-blotting(WB),and immunohistochemistry(IHC),while its prognostic significance was assessed employing Kaplan–Meier survival analysis(KMSA),log-rank test(LR),and Cox’s regression analysis(CRA).Bioinformatics techniques facilitated the prediction and analysis of its plausible mechanisms in LM,further substantiated by in vitro and in vivo experiments utilizing FaDu cell ***:HSP90AA1 is substantially upregulated in HPSCC with LM and is identified as an independent prognostic risk *** down-regulation of HSP90AA1 can achieve inhibition of tumor cell proliferation,migration and *** in vivo experiments and Bioinformatics exploration hint at promoting LM by Epithelial-mesenchymal transition(EMT),regulated by ***:HSP90AA1,by controlling EMT,can foster LM in *** finding sets the foundation for delving into new therapeutic targets for HPSCC.

关键词： Hypopharyngeal squamous cell carcinoma Lymphatic metastasis HSP90AA1 EMT HNSCC

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects and mechanism of Chinese medicine Jiawei Yupingfeng in a mouse model of allergic rhinitis

引用

Journal of Integrative Medicine 2021年第4期19卷 354-361页

作者： Hui Chen Wei Feng Ye Lu Yi Yang Zhong-hai Xin Man Li Li Xin Ying-di Gong department of otolaryngology Wangjing HospitalChina Academy of Chinese Medical SciencesBeijing 100102China

Objective: Chinese medicine has the potential to modulate allergic rhinitis(AR). There have been studies investigating the treatment efficacy of Yupingfeng San, alone or in combination with other ingredients, in AR, though few have studied the potential mechanisms of these drugs. In the present study, we measured the effects of Jiawei Yupingfeng(JWYPF), a traditional Chinese medicine formula, on mice with ovalbumin-induced AR and explored its underlying mechanism of ***: Forty BALB/c mice were randomly divided into normal control, allergy control and two treatment groups of ten mice each. In the normal control group, mice were sensitized and challenged with saline. The mice in the allergy control and treatment groups were sensitized and challenged with ovalbumin and aluminum hydroxide gel. The treatments of JWYPF and Nasonex were administered intranasally in the AR mice for one week. Several signs of allergic inflammation, such as nasal eosinophils and inflammatory cytokines, were measured to determine the underlying ***: Mice in the JWYPF and Nasonex groups had significantly lower AR symptom scores than those in the allergy control group(the mean differences between JWYPF and the allergy control, and Nasonex and the allergy control were -2.00 ± 0.35 and -2.40 ± 0.32). After treatment with JWYPF and Nasonex, the levels of ovalbumin-specific Ig E and histamine were significantly reduced, as were the levels of interlukin-4 and transforming growth factor-b, while interferon-c levels were increased(all P < 0.0001,vs. allergy control). These two treatments also significantly inhibited eosinophil and mast cell infiltration into the nasal cavity but were not statistically different from ***: JWYPF has a potential therapeutic effect on AR via adjusting the rebalance of T helper 1 and T helper 2.

关键词： Chinese medicine Jiawei Yupingfeng Allergic rhinitis Mouse Inflammatory cytokines

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Quantitative analysis of the ribbon synapse number of cochlear inner hair cells in C57BL/6J mice using the three-dimensional modeling method

引用

Science China(Life Sciences) 2009年第9期52卷 807-812页

作者： LIU Ke,LI ShuNa & JIANG XueJun department of otolaryngology,the First Affiliated Hospital,China Medical University,Shenyang 110001,China department of otolaryngology the First Affiliated Hospital China Medical University Shenyang China

In mammals,the ribbon synapses of cochlear inner hair cells are a synaptic structure of the first sensory nerve in the pathway of acoustical signal transmission to the acoustic center,and it is directly involved in voice coding and neurotransmitter release. It is difficult to quantitatively analyze the ribbon synaptic number only using an electron microscope,because the ribbon synaptic number is relatively limited and their location is deep. In this study,the specific presynaptic structure-RIBEYE,and non-specific postsynaptic structure-GluR 2 & 3 in C57BL/6J mouse basilar membrane samples were treated by immunofluorescent labeling. Serial section was performed on the samples using a laser scanning confocal microscope,and then the serial sections were used to build three-dimensional models using 3DS MAX software. Each fluorescein color pair indicates one synapse,so the number of ribbon synapses of inner hair cells is obtained. The spatial distribution and the number of ribbon synapses of cochlear inner hair cells were clearly shown in this experiment,and the mean number of ribbon synapses per inner hair cell was 16.10±1.03. Our results have demonstrated the number of ribbon synapses is accurately calculated by double immunofluorescent labeling to presynaptic and postsynaptic structures,serial sections obtained using a laser scanning confocal microscope,and three-dimensional modeling obtained using 3DS MAX software. The method above is feasible and has important significance for further exploring the mechanism of sensorineural deafness.

关键词： inner hair cells Ribbon synapses laser scanning confocal microscope three-dimensional modeling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Diagnosis and Treatment of Auditory Neuropathy and Related Research

引用

Journal of Otology 2012年第2期7卷 86-91页

作者： JI Fei YANG Shiming department of otolaryngology Head and Neck Surgery Chinese PLA General Hospital Chinese PLA Institute of otolaryngology

Introduction Deafness is one of the most common otologic diseases and a major disease that greatly impacts the Chinese population. From the Second National Sample Survey of Disabled Persons, it is estimated that there... 详细信息

关键词： Diagnosis and Treatment of Auditory Neuropathy and Related Research ABR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

EFFECTS OF MODERATE NOISE EXPOSURE ON HEARING FUNCTION IN C57BL/6J MICE

引用

Journal of Otology 2014年第2期9卷 81-85页

作者： SHI Chuang SHI Lei JIANG Xuejun YANG Shiming LIU Ke department of otolaryngology Head and Neck Surgery The First Affiliated Hospital of China Medical University department of otolaryngology Head and Neck surgery Shiyan Taihe Hospital department of otolaryngology Head and Neck surgery Institute of OtolaryngologyChinese PLA General Hospital

Objective To study characteristics of hearing loss after exposure to moderate noise exposure in C57BL/6J mice. Methods Male C57BL/6J mice with normal hearing at age of 5-6 weeks were chosen for this study. The mice were randomly sclccted to be studied immediately after exposure （Group P0）, or 1 day （Group P1）, 3 days （Group P3）, 7 day （Group P7） or 14 days （P14） after exposure. Their before exposure condition served as the normal control. All mice were exposed to a broad-band white noise at 100 dB SPL for 2 hours, ABR thresholds were used to estimate hearing status at each time point. Results ABR threshold elevation was seen at every tested frequency at P0 （P〈0.01）. Elevation at high-frequencies （16 kHz and 32 kHz） was greater than at lower frequencies （4 kHz and 8 kHz, P〈0.05）. From P1 to P14, ABR thresholds continuously improved, and there was no significant difference between P14 and before exposure （P〉0.05）. Conclusion There is a frequency specific re- sponse to 100 dB SPL broad-band white noise in C57BL/6J mice, with the high-frequency being more susceptible. Hearing loss induced by moderate noise exposure appears reversible in C57BL/6J mice.

关键词： C57BL/6J mice Noise exposure Hearing loss Temporary noise-induced hearing loss ABR threshold shift

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hearing evaluation after successful myringoplasty

引用

Journal of Otology 2017年第4期12卷 192-197页

作者： Mohammed Radef Dawood otolaryngology department College of Medicine Mustansiriyah University Baghdad Iraq

Objectives: To assess postoperative hearing level, and factors that may have influence hearing improvement after ***: Twenty six cases of successful myringoplasty were included in this prospective study. Patient parameters including age, gender, size and site of the perforation, mastoid status, and etiology were evaluated. Hearing levels were assessed as the mean air conduction(AC), and airbone gap(ABG) at 500, 1000, and 2000 Hz, and their relation with aforementioned parameters were ***: The mean AC hearing gain was 22.373 dB and mean ABG reduction was 20.733 dB. The maximum AC hearing gain was 25.93 dB for subtotal perforation and 26.24 dB for big central perforation, and the maximum ABG reduction was 25.63 dB for subtotal perforation and 24.20 for big central perforation. Mean AC hearing gain was 23.01 dB, 22.72 dB, and 21.39 dB for 500, 1000, and 2000 Hz, respectively, and mean ABG reduction was 21.52 dB, 20.79 dB, and 19.86 dB for 500, 1000, and 2000 Hz, respectively. Patient age, gender, mastoid status and etiology did not seem to have any bearing on postoperative hearing ***: While patient parameters do not seem to correlate with hearing improvement following myringoplasty, the size and location of perforation appear to have an impact on postoperative hearing outcomes. Most hearing improvement appears to occur at 500 Hz.

关键词： Myringoplasty Hearing loss Tympanic membrane

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Model of Spontaneous Chronic Otitis Media with Effusion in Mice with ENU Induced Mutations

引用

Journal of Otology 2010年第2期5卷 76-89页

作者： Robert-connelly department of otolaryngology Case Western Reserve University

Background Chronic otitis media(COM) is a significant clinical *** the mechanisms of COM is critical for its control and ***,little is known of the processes leading to COM as a result of lack of animal models of N-ethyl-N-nitrosourea(ENU) induced mutations in otitis media with effusion(OME).Methods Otoscopy and auditory brain response(ABR) evaluation were carried out under sedation in Nmf391nmf/nmf mice of 2,4,6 and 8 months of *** mice were killed for study of middle and inner ear *** Tympanic membrane visualization and ABR thresholds in 1-to 8-month-old Nmf391nmf/nmf mice showed spontaneous OME and inner ear diseases in approximately 100% of the *** significant elevation of ABR thresholds suggested a sensorineural component in hearing loss in addition to the conductive *** and inner ear histology showed various degrees of outer hair cells loss and middle ear inflammation in all the mice,but no inflammation cells in the inner *** ABR threshold at 32 kHz was significantly *** This study shows histopathologic changes in the Nmf391nmf/nmf mouse model of COM with effusion that have not been reported in human *** ENU induced mutation model of COM will be valuable for the characterization of middle ear inflammation and inner ear disease processes that are induced by middle ear *** propose that COM with effusion in this ENU induced mutation model is the cause of the cochlea hair cells damage.

关键词： Otitis media mouse model genetic animal models inflammation auditory brainstem response.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：