检索结果-南通市图书馆

Evaluation of Fluorescent Stains for Viability Assessment of the Potato Cyst Nematodes Globodera pallidaand G. ellingtonae

引用

Advances in Bioscience and Biotechnology 2019年第8期10卷 244-258页

作者： Syamkumar Sivasankara Pillai Louise-Marie Dandurand department of Entomology Plant Pathology and Nematology University of Idaho Moscow USA

Potato cyst nematodes (PCNs), Globodera pallida and Globodera rostochiensis, are quarantine pests of potato which cause significant damage to production and farm gate revenue worldwide. Accurately assessing viability of PCN eggs is important for eradication and management programs. The goal of this study was to develop a quick and reliable fluorescent staining method to evaluate viability of G. pallida and Globodera ellingtonae eggs. The staining efficiency of eight fluorescent stains was evaluated using G. pallida eggs compared with the conventional Meldola’s Blue (MB) staining method. The staining efficiency of the fluorescent stains ranged from 80.33 ± 2.99 (Sytox Green) to 100% (Acridine Orange) for non-viable eggs. Two stains were further evaluated for their efficiency in assessing viability of encysted eggs from five different greenhouse-reared G. pallida cyst sources which contained both viable and non-viable eggs. For the G. pallida cyst sources, viability ofencysted eggs were estimated to be 41.02 ± 3.81 to 62.66% ± 3.12% when stained with Acridine Orange (AO) and 79.52% ± 1.54% viability for G. ellingtonae. Both staining time and stain concentration were significant for staining efficiency of released and encysted eggs. Staining time and concentration were optimized for released eggs at 4 h at 10 μg/ml and for encysted eggs at 16 h at 25 μg/ml respectively for AO. Fluorescent stains accurately and rapidly assessed percent egg viability and were determined to be as sensitive as a seven-day incubation with the Meldola’s Blue staining method.

关键词： Globodera pallida Globodera ellingtonae Nematode Viability Assessment Fluorescent Stains Meldola Blue

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Morphology and Taxonomy of Xiphinema(Nematoda：Longidoridae) Occurring in Arkansas,USA

引用

江西农业大学学报 2010年第5期32卷 928-945页

作者：叶为民 ROBBINS R.T. department of Plant Pathology Nematology Laboratory2601 N.Young Ave.University of Arkansas Present address:Nematode Assay Laboratory North Carolina Department of Agriculture and Consumer Services

In a survey,primarily from the rhizosphere of hardwood trees growing on sandy stream banks,for longidorids,828 soil samples were collected from 37 Arkansas counties in 1999—*** hundred twenty-seven populations of Xiphinema were recovered from 452 of the 828 soil samples(54.6%),including 71 populations of *** sensu lato,33 populations of ***,23 populations of *** and one population of *** morphological and morphometric characteristics of these Arkansas species are *** and morphometric characteristics are also given for two populations of *** from Hawaii and North Carolina.

关键词： Arkansas morphology SEM survey taxonomy Xiphinema americanum X.bakeri X.chambersi X.krugi.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Biological Control of Root-Knot Nematode Meloidogyne incognita in Psoralea corylifolia Plant by Enhancing the Biocontrol Efficacy of Trichoderma harzianum Using Press Mud

引用

Phyton-International Journal of Experimental Botany 2022年第8期91卷 1757-1777页

作者： Yasar Nishat Mohammad Danish Heba I.Mohamed Hisamuddin Shaikh Abeer Elhakem Section of Plant Pathology and nematology Department of BotanyAligarh Muslim UniversityAligarh202002India department of Biological and Geological Sciences Faculty of EducationAin Shams UniversityCairo11341Egypt department of Biology College of Science and Humanities in Al-KharjPrince Sattam Bin Abdulaziz UniversityAl-Kharj11942Saudi Arabia

Meloidogyne incognita is a plant pathogen causing root-knot disease and loss of crop *** present study aimed to use Trichoderma harzianum as a biocontrol agent against plant-parasitic nematodes and used press mud,which is a solid waste by-product of sugarcane,as a biocontrol agent and ***,the combined application of *** and press mud may enhance nematode control and plant *** analysis of press mud using scanning electron microscopy(SEM)integrated with an Energy Dispersive X-ray(EDX)analyzer revealed the presence of different elements such as C,O,Mg,Si,P,K,Ca,Cu and *** addition,a greenhouse study was conducted to investigate the combined effects of press mud and *** on *** reproduction and growth and the biochemical features of Psoralea *** results showed that plant length,dry biomass,leaf area,the number of seeds per plant,chlorophyll a,chl b,carotenoid content,nitrate reductase,carbonic anhydrase,and nitrogen content were significantly increased(P≤0.05)in the T2 plants(plants were treated with 100 g of press mud+50 mL *** before one week of *** inoculation),over inoculated plants(IC).Antioxidant enzyme activity of ascorbate peroxidase(APX),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)in the foliage of *** was significantly increased when plants were treated with press mud+***.A significant reduction in the number of egg masses,nematode population,and root-knot index(RKI)was found in plants with T2 *** results suggest that the combined application of *** and press mud has the potential to control the *** infection and can be used as an environmentally safe alternative to chemical nematicides and also help in the removal of sugarcane waste that causes environmental pollution.

关键词： Growth improvement antioxidant enzymes nitrate reductase carbonic anhydrase root-knot index

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparative morphometric and chemical analyses of phenotypes of two invasive ambrosia beetles （Euwallacea spp.） in the United States

引用

Insect Science 2017年第4期24卷 647-662页

作者： Yigen Chen Paul L. Dallara Lori J. Nelson Tom W. Coleman Stacy M. Hishinuma Daniel Carrillo Steven J. Seybold department of Entomology and nematology University of California Davis USA USDA Forest Service Pacific Southwest Research Station Davis USDA Forest Service Forest Health Protection San Bemardino California USA Tropical Research and Education Center University of Florida Homestead Florida USA

The polyphagous shot hole borer （PSHB）, Euwallacea sp., was first detected in 2003 in Los Angeles County, California, USA. Recently, this invasive species has become a major pest of many hardwood trees in urban and wildland forests throughout southern California. PSHB is nearly identical in morphology and life history to the tea shot hole borer （TSHB）, Euwallaceafornicatus, an invasive pest of hardwoods in Florida, USA and many other parts of the world. However, molecular studies have suggested that the taxa are different species. We conducted morphometric and chemical analyses of the pheno- types of Euwallacea sp. collected in southern California （Los Angeles County） and E. fornicatus collected in Florida （Miami-Dade County）. Our analyses indicated that PSHB has 3 larval instars. The third larval instar was separated from the first 2 instars by head capsule width with 0 probability of misclassification. The body length, head width, and pronotal width of PSHB adult males were significantly less than those of females. Head width and pronotal width of female PSHB were significantly less than those of female TSHB. In contrast, body length, and ratio of body length to pronotal width of female PSHB were significantly greater than those of female TSHB. However, females of these 2 species could not be separated completely by these 4 measurements because of the overlapping ranges. Cuticular hydrocarbons detected in both species were exclusively alkanes （i.e., n-alkanes, monomethylalkanes, dimethylalkanes, and trimethylalkanes）. Cuticular hydro- carbon profiles of PSHB males and females were similar, but they both differed from that of TSHB females. Cuticular hydrocarbons of PSHB were predominantly internally branched dimethylalkanes with backbones of 31 and 33 carbons, whereas cuticular hydro- carbons of TSHB females were dominated by internally branched monomethylalkanes and dimethylalkanes with backbones of 28 and 29 carbons. Multiple compounds within these classes appear

关键词： cuticular hydrocarbons Euwallaceafornicatus larval instars polyphagousshot hole borer Scolytidae tea shot hole borer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genetic variation and potential coinfection of Wolbachia among widespread Asian citrus psyllid (Diaphorina citri Kuwayama) populations

引用

Insect Science 2019年第4期26卷 671-682页

作者： Chia-Ching Chu Mark Hoffmann W. Evan Braswell Kirsten S. Pelz-Stelinski department of Entomology and nematology University of Florida Gainesville Florida USA Citrus Research and Education Center University of Florida Lake Alfred Florida USA 3department of Horticultural Sciences North Carolina State University Raleigh North Carolina USA Center for Plant Health Science and Technology USDA APHIS Edinburg Texas USA

Wolbachia can profoundly influence the survival, reproduction, and defenses of insect hosts. These interactions could potentially be harnessed for managing pests or insecttransmitted diseases. Diaphorina citri Kuwayama is a phloem-feeding pest capable of transmitting the putative causal agent of citrus greening, Candidatus Liberibacter asiaticus (CLas). Like many insects, D. citri is also infected with Wolbachia (wDi). Recent studies indicate that the relative abundance of wDi could be associated with the abundance of CLas, and that wDi may contribute to regulating expression of phage lytic cycle genes in CLas, suggesting the need for better understanding of wDi biology in general. This study investigated the genetic diversity of wDi among D. citri in populations spanning eleven countries and two U.S. territories. Six Wolbachia genes, wsp, ***, gatB, and hep A, were sequenced and compared across samples. Two prevalent wDi strains were identified across the samples, and screening of clone libraries revealed possible coinfection of wDi strains in specific populations. D. citri mitochondrial cytochrome oxidase subunit I gene (mtCOI) were more divergent between D. citri populations that were infected with different wDi strains or had different infection statuses (single infection vs. coinfection). While we could not eliminate the possibility that maternal transmission may contribute to such patterns, it is also possible that wDi may induce cytoplasmic incompatibility in their host. These fin dings should contribute to the understanding of wDi population ecology, which may facilitate manipulation of this endosymbiont for management of citrus greening disease worldwide.

关键词： bacteria genetic diversity Huanglongbing plant disease superinfection vertically transmitted endosymbionts

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exploring and integrating cellulolytic systems of insects to advance biofuel technology

引用

Insect Science 2010年第3期17卷 163-165页

作者： Jian-Zhong Sun Michael E. Scharf Biofuels Institute School of the Environment Jiangsu University Zhenjiang 212013Jiangsu China Entomology and nematology department PO Box 110620 Bldg 970 Natural Area Dr. University of FloridaGainesville FL 32611-0620 USA

In line with the requirements for sustainable economies and clean environments, cellulose-based biofuels have recently received tremendous attention both in industry and academic communities worldwide. Alternative and renewable fuels derived from lignocellulosic biomass offer the potential to reduce our dependence on fossil fuels and mitigate global climate change.

关键词： Exploring integrating cellulolytic systems advance biofuel

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Factors shaping life history traits of two proovigenic parasitoids

引用

Integrative zoology 2018年第3期13卷 297-306页

作者： Michal SEGOLI Shucun SUN Dori ENAVA Jay A.ROSENHEIM Mitrani department of Desert Ecology The Jacob Blaustein Institutes for Desert ResearchBen-Gurion University of the NegevMidreshet Ben-GurionIsrael department of Ecology School of Life ScienceNanjing UniversityNanjingChina Embrapa Clima Temperado(Embrapa Temperate Agriculture) PelotasRio Grande do SulBrazil department of Entomology and nematology University of California DavisDavisCaliforniaUSA

What shapes the relative investment in reproduction versus survival of organisms is among the key questions in life *** insects mature all their eggs prior to emergence and are short lived,providing a unique opportunity to quantify their lifetime investments in the different *** investigated the initial eggloads and longevity of 2 proovigenic parasitoid wasps:Anagrus erythroneurae and Anagrus daanei,(Hymenoptera:Mymaridae)that develop within leafhopper eggs in both agricultural vineyards and natural riparian habitats in Northern *** collected Vitis *** containing developing parasitoids from 3 natural sites(Knight Landing,American River and Putah Creek)and 3 agricultural vineyards(Solano Farm,Davis Campus and Village Homes).We recorded eggloads at parasitoid emergence and female parasitoid longevity with or without *** predicts that parasitoids from vineyards(where hosts are abundant)would have higher initial eggloads and lower longevity compared with parasitoids from riparian habitats(where hosts are scarce).Although host density and parasitoid eggloads were,indeed,higher in vineyards than in riparian habitats,parasitoid longevity did not follow the predicted *** without feeding differed among field sites,but it was not affected by habitat type(natural vs agricultural),whereas longevity with feeding was not significantly affected by any of the examined ***,longevity was positively,rather than negatively,correlated with eggloads at the individual level,even after correcting for parasitoid body *** combined results suggest a more complex allocation mechanism than initially predicted,and the possibility of variation in host quality that is independent of size.

关键词： Anagrus parasitoids eggload leafhoppers life-history tradeoffs longevity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fungi associated with wheat seed discolouration and abnormalities in in-vitrostudy

引用

agricultural Sciences 2013年第9期4卷 516-520页

作者： Neha Pathak Razia. K. Zaidi Section of Plant Pathology and nematology Department of Botany Aligarh Muslim University Aligarh India

The main aim of present study is to ascertain the fungal species and their effect on germination associated with wheat seeds. Seeds of three varieties WH896, PBW-373 and HD264 of wheat (Triticum aestivum) were collected from Quarsi Agriculture Farm Aligarh. These three seed samples of wheat showing different forms of discouloration and abnormalities were screened for associated fungi. Microscopic examination of wheat seeds reveals that seeds of all the varieties of wheat possess injuries to varying extent. Detailed examination of the seeds has shown that the seeds can be classified on the basis of extent of injury in the three categories viz., seeds having minor cracks, cracks without exposed embryo and cracks with exposed embryo. Seed soaking and washing techniques were also employed. Fusarium moniliforme and Alternaria alternata were isolated from all the categories of seed tested. Floating mycelial bits and conidia of Alternaria, Fusarium, Drechslera, Curvularia lunata, Mucor were found in all the three varieties of wheat. The seeds were subjected to visual observation and examination under stereoscopic microscope. These findings are to study and detect the phytopathogenic mycoflora which causes damage and loss to our seeds and crops.

关键词： Wheat Seed-Borne Fungi Incidence Discolouration Wrinkled Seeds

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Occurrence of Root-Knot and Reniform Nematodes in Ornamental Plants Grown in Aligarh Muslim University Campus, India

引用

American Journal of Plant Sciences 2014年第13期5卷 1885-1888页

作者： Aasia Rashid Bhat Tabreiz Ahmed Khan Umar Farooq Section of Plant Pathology and nematology Department of Botany Aligarh Muslim University Aligarh India

An extensive survey of about 50 ornamental plants growing in A.M.U. campus were carried out to find out the infection of root-knot (Meloidogyne spp.) and reniform nematodes (Rotylenchulus reniformis). Out of 50 species of ornamental plants studied, 29 species of these plants were found to be infected with root-knot nematodes (Meloidogyne spp.) and 15 species were infected with reniform nematode (Rotylenchulus reniformis). The highest percentage of infection of root-knot nematodes was observed in Impatiens balsamina (100%) and reniform nematode was observed in Hibiscus rosa-sinensis (56%). Among the Meloidogyne spp. the highest percentage of infection of M. incognita was observed in Pseuderanthemum atropurpureum (80%) whereas, the highest percentage of infection of M. javanica and M. arenaria was observed in Mirabilis jalapa (72%) and Celosis cristata (28%).

关键词： Root-Knot Nematode Reniform Nematode Ornamental Plants

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Plasticity of mycangia in Xylosandrus ambrosia beetles

引用

Insect Science 2019年第4期26卷 732-742页

作者： You Li Yong-Ying Ruan Edward L. Stanley James Skelton Jiri Hulcr School of Forest Resources and Conservation University of Florida Gainesville Florida USA School of Applied Chemistry and Biological Technology Postdoctoral Innovation Practice Base Shenzhen Polytechnic Shenzhen. Guangdong. China Florida Museum of Natural History University of Florida Gainesville Florida USA Entomology and nematology department University of Florida Gainesville Florida USA

Insects that depend on microbial mutualists evolved a variety of organs to transport the microsymbionts while dispersing. The ontogeny and variability of such organs is rarely studied, and the microsymbiont*s effects on the animal tissue development remain unknown in most cases. Ambrosia beetles (Coleoptera: Curculionidae: Scolytinae or Platypodinae) and their mutualistic fungi are an ideal system to study the animalfungus interactions. While the interspecific diversity of their fungus transport organ一 mycangia—is well-known, their developmental plasticity has been poorly described. To determine the ontogeny of the mycangium and the influence of the symbiotic fungus on the tissue development, we dissected by hand or scanned with micro-CT the mycangia in various developmental stages in five Xylosandrus ambrosia beetle species that possess a large, mesonotal mycangium: Xylosandrus amputatus. Xylosandrus compactus, Xylosandrus crassiusculus, Xylosandrus discolor, and Xylosandrus germanus. We processed 181 beetle samples from the United States and China. All five species displayed three stages of the mycangium development:(1) young teneral adults had an empty, deflated and cryptic mycangium without fungal mass;(2) in fully mature adults during dispersal, the promesonotal membrane was inflated, and most individuals developed a mycangium mostly filled with the symbiont, though size and symmetry varied;and (3) after successful establishment of their new galleries, most females discharged the bulk of the fun gal inoculum and deflated the mycangium. Experimental aposymbiotic individuals demonstrated that the pronotal membrane invaginated independently of the presence of the fungus, but the fungus was required for inflation. Mycangia are more dynamic than previously thought, and their morphological changes correspond to the phases of the symbiosis. Importantly, studies of the fungal symbionts or plant pathogen transmission in ambrosia beetles need to consider which developmen

关键词： callow micro-CT mycangium symbiont-induced symbiosis teneral

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：