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development and Application of Detection Methods for Capture and Transcription Elongation Rate of Bacterial Nascent RNA

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生物化学与生物物理进展 2024年第9期51卷 2249-2260页

作者： LI Yuan-Yuan WANG Yu-Ting WU Zi-Chun LI Hao-Xuan FEI Ming-Yue SUN Dong-Chang GUALERZI O.Claudio FABBRETTI Attilio GIULIODORI Anna Maria MA Hong-Xia HE Cheng-Guang Engineering research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical development College of Life SciencesJilin Agricultural UniversityChangchun 130118China School of Biosciences and veterinary Medicine University of CamerinoCamerino(MC)62032Italy College of Biotechnology and Bioengineering Zhejiang University of TechnologyHangzhou 310014China

Objective Detection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability,health,and metabolic *** exposure to environmental stimuli,both the internal reference gene and target gene would be *** a result,it is imperative to consider the accurate capture of nascent RNA and the detection of transcriptional levels of RNA following environmental *** study aims to create a Click Chemistry method that utilizes its property to capture nascent RNA from total RNA that was stimulated by the *** The new RNA was labeled with 5-ethyluridine(5-EU)instead of uracil,and the azido-biotin medium ligand was connected to the magnetic sphere using a combination of“Click Chemistry”and magnetic bead *** the new RNA was captured and the transcription rate of 16S rRNA was detected by fluorescence molecular beacon(M.B.)and quantitative reverse transcription PCR(qRT-PCR).Results The bacterial nascent RNA captured by“Click Chemistry”screening can be used as a reverse transcription template to form *** with the fluorescent molecular beacon M.B.1,the synthesis rate of rRNA at 37℃is 1.2 times higher than that at 15℃.The 16S rRNA gene and cspI gene can be detected by fluorescent quantitative PCR,it was found that the measured relative gene expression changes were significantly enhanced at 25℃and 16℃when analyzed with nascent RNA rather than total RNA,enabling accurate detection of RNA transcription *** Compared to other article reported experimental methods that utilize screening magnetic columns,the technical scheme employed in this study is more suitable for bacteria,and the operation steps are simple and easy to implement,making it an effective RNA capture method for researchers.

关键词： nascent RNA selection Click Chemistry fluorescence molecular beacon

A dual-beam piezo-magneto-elastic wake-induced vibration energy harvesting system for high-performance wind energy harvesting

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Science China(Technological Sciences) 2024年第1期67卷 221-239页

作者： MA XiaoQing ZHANG Hang MARGIELEWICZ Jerzy GĄSKA Damian WOLSZCZAK Piotr LITAK Grzegorz ZHOU ShengXi School of Aeronautics Northwestern Polytechnical UniversityXi’an 710072China research&development Institute in Shenzhen Northwestern Polytechnical UniversityShenzhen 518057China Faculty of Transport and Aviation Engineering Silesian University of TechnologyKrasińskiego 8Katowice 40-019Poland department of Automation Lublin University of TechnologyNadbystrzycka 36Lublin 20-618Poland

Wind-induced vibration energy harvesting has a great potential for utilizing wind energy to supply power for low-powered *** improve the working performance of energy harvesters effectively,a suitable structural design is *** paper proposes a dual-beam piezo-magneto-elastic wake-induced vibration energy harvesting system to enhance the functional performance of aeroelastic energy harvesters in environments with variable wind *** system contains two piezoelectric beams coupled by magnets(forming upstream and downstream energy harvesters),and each beam is attached with a foam cylinder.A corresponding dynamic model is provided,and output characteristics are obtained at different wind *** and experimental verification indicate that both upstream and downstream energy harvesters can realize efficient energy *** the wind speed exceeds a certain critical value,the amplitudes of the system’s displacement and voltage are *** wind speed threshold value is approximately 1.25 m/*** the wind speed and magnet spacing are 10.2 m/s and 20 mm,respectively,the output power of the system reaches 4.9×10^(−4)***,the wind speed threshold value of the proposed system can be adjusted by an equivalent nonlinear restoring force.

关键词： dual-beam structure piezoelectric energy harvester theoretical model nonlinear dynamics

Study on the tunnel shape and soil-lining interaction influencing the lining behavior under seismic loading

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Earthquake Engineering and Engineering Vibration 2024年第4期23卷 845-862页

作者： Van Vi Pham Ngoc Anh Do Piotr Osinski Ngoc Thai Do Daniel Dias Sustainable development in Underground Engineering research Team Hanoi University of Mining and GeologyHanoiVietnam department of Underground and Mining Construction Faculty of Civil EngineeringHanoi University of Mining and GeologyHanoiVietnam department of Geotechnical Engineering Institute of Civil EngineeringWarsaw University of Life Sciences-SGGWWarszawaPoland Laboratory 3SR Grenoble Alpes UniversityGrenoble 38400France

The response of tunnels subjected to seismic loading is a complex mechanism and depends not only on the seismic nature but also on tunnel structure and surrounding soil *** individual behavior of circular,rectangular,and sub-rectangular tunnels subjected to seismic loadings has already been studied in the *** the present research,two case scenarios of circular,rectangular tunnels and four sub-rectangular shaped tunnels,with similar cross-section areas,were adopted to perform a comprehensive numerical *** purpose of the study was to determine the mechanical behavior of tunnels of different shapes,depending upon seismic *** were performed by considering the influence of soil-lining interaction,soil parameters,and lining thickness,as well as lining *** were performed for no-slip and full-slip *** results indicate that the tunnel shape design is of great importance when regarding the mechanical behavior of the surrounding *** concerns no-slip as well as full-slip soil-lining interaction,especially when the lining is subjected to seismic ***,it is shown that changes in incremental bending moments for circular,rectangular and sub-rectangular tunnels that depend upon the soil-lining interaction conditions differ significantly.

关键词： tunnel shape soil-lining interaction seismic loading internal forces numerical analysis

Current understanding of ELF4 deficiency:a novel inborn error of immunity

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World Journal of Pediatrics 2024年第5期20卷 444-450页

作者： Hong-Qiang Du Xiao-Dong Zhao department of Rheumatology&Immunology National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChongqing Key Laboratory of Child Rare Diseases in Infection and ImmunityChildren’s Hospital of Chongqing Medical UniversityZhongshan Er Road 136Yuzhong DistrictChongqingChina

Background ELF4 deficiency has been recently recognized as a novel disorder within the spectrum of inborn errors of immunity(IEIs),specifically categorized as a“disease of immune dysregulation.”Cases of this condition,reported by our team and others,are very limited *** such,our current knowledge of this new disease remains *** review aims to provide a brief overview of the clinical manifestations,pathogenesis,and treatment strategies for this novel *** sources A comprehensive review was conducted after an extensive literature search in the PubMed/Medline database and websites concerning transcriptional factor ELF4 and reports concerning patients with ELF4 *** search strategy was“ELF4 OR ETS-related transcription factor Elf-4 OR EL4-like factor 4 OR myeloid Elf-1-like factor”as of the time of manuscript *** The current signature manifestations of ELF4 deficiency disorder are recurrent and prolonged oral ulcer,abdominal pain,and diarrhea in pediatric *** some cases,immunodeficiency and autoimmunity can also be *** Sanger sequencing or whole exome sequencing can be used to detect variation in ELF4 *** blotting for ELF4 expression of the patient’s cells can confirm the pathogenic effect of the *** fully confirm the pathogenicity of the variant,further functional test is strongly *** and biologics are the mainstream management of ELF4 deficiency *** Pediatric males presenting with recurring ulcerations in digestive tract epithelium with or without recurrent fever should be suspected of *** atypical presentations are prominent,variations in ELF4 gene should be carefully evaluated functionally due to the complex nature of ELF4 *** of treating DEX includes use of glucocorticoid and biologics and more precise treatment needs more patients to identify and further mechanistic study.

关键词： ELF4 transcription factor Immune dysregulation Inborn errors of immunity Mechanism Recurrent infections

Single transmembrane GPCR modulating proteins:neither single nor simple

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Protein & Cell 2024年第6期15卷 395-402页

作者： Meng Wang Jianjun Lyu Chao Zhang department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Hubei Topgene research Institute of Hubei Topgene Biotechnology Co. LtdEast Lake High-Tech Development ZoneWuhan 430205China Fundamental research Center Shanghai Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center)Tongji UniversityShanghai 200092China

The discovery of G-protein coupled receptor(GPCR)accessory proteins has fundamentally redefined the pharmacological concept of GPCR signaling,demonstrating a more complex molecular basis for receptor specificity on the plasma membrane and impressionable downstream intracellular *** accessory proteins not only contribute to the proper folding and trafficking of receptors but also exhibit selectable receptor preferences.

关键词： specificity GPCR neither

Natural medicine can substitute antibiotics in animal husbandry:protective effects and mechanisms of rosewood essential oil against Salmonella infection

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Chinese Journal of Natural Medicines 2024年第9期22卷 785-796页

作者： WANG Lanqiao FANG Juan WANG Heng ZHANG Baoyu WANG Nan YAO Xinyu LI He QIU Jiazhang DENG Xuming LENG Bingfeng WANG Jianfeng TAN Wenxi ZHANG Qiaoling State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases Key Laboratory for Zoonosis Research of the Ministry of EducationInstitute of Zoonosisand College of Veterinary MedicineJilin UniversityChangchun 130062China Shenzhen Beichen Biotech Co. Ltd.Shenzhen 518057China department of Obstetrics and Gynecology The Second Hospital of Jilin UniversityChangchun 130041China

Aniba rosaeodora essential oil(RO) has been traditionally used in natural medicine as a substitute for antibiotics due to its notable antidepressant and antibacterial properties. Salmonella, a prevalent pathogen in foodborne illnesses, presents a major challenge to current antibiotic treatments. However, the antibacterial efficacy and mechanisms of action of RO against Salmonella spp. remain underexplored. This study aims to elucidate the chemical composition of RO, evaluate its antibacterial activity and mechanisms against Salmonella in vitro, and further delineate its anti-inflammatory mechanisms in vivo during Salmonella infection. Gas chromatography-mass spectrometry(GC-MS) was utilized to characterize the chemical constituents of RO. The antibacterial activity of RO was assessed using minimal inhibitory concentration(MIC) and time-kill assays. Various biochemical assays were employed to uncover the potential bactericidal mechanisms. Additionally, mouse and chick models of Salmonella infection were established to investigate the prophylactic effects of RO treatment. RO exhibited significant antibacterial activity against both Gram-positive and Gram-negative bacteria, with an MIC of 4 mg·mL^(-1) for Salmonella spp. RO treatment resulted in bacterial damage through the disruption of lipid and purine metabolism. Moreover, RO reduced injury and microbial colonization in infected mice and chicks. RO treatment also modulated the host inflammatory response by inhibiting proinflammatory pathways. In conclusion, our findings demonstrate that RO is effective against Salmonella infection, highlighting its potential as an alternative to antibiotics for antibacterial therapy.

关键词： Rosewood essential oil Salmonella Aniba rosaeodora Antibacterial effect Anti-inflammation Anti-infection

Cell division cyclin 25C knockdown inhibits hepatocellular carcinoma development by inducing endoplasmic reticulum stress

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World Journal of Gastroenterology 2024年第19期30卷 2564-2574页

作者： Yan-Fei Li Fang-Yuan Zheng Xin-Yu Miao Hai-Long Liu Yao-Yao Zhang Nai-Xia Chao Fa-Rong Mo School of Basic Medical Sciences Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Key Laboratory of Biological Molecular Medicine research(Guangxi Medical University) Education Department of Guangxi Zhuang Autonomous RegionNanning 530021Guangxi Zhuang Autonomous RegionChina Key Laboratory of Human development and Disease research(Guangxi Medical University) Education Department of Guangxi Zhuang Autonomous RegionNanning 530021Guangxi Zhuang Autonomous RegionChina

BACKGROUND Cell division cyclin 25C(CDC25C)is a protein that plays a critical role in the cell cycle,specifically in the transition from the G2 phase to the M *** research has shown that CDC25C could be a potential therapeutic target for cancers,particularly for hepatocellular carcinoma(HCC).However,the specific regulatory mechanisms underlying the role of CDC25C in HCC tumorigenesis and development remain incompletely *** To explore the impact of CDC25C on cell proliferation and apoptosis,as well as its regulatory mechanisms in HCC *** Hepa1-6 and B16 cells were transduced with a lentiviral vector containing shRNA interference sequences(LV-CDC25C shRNA)to knock down ***,a xenograft mouse model was established by subcutaneously injecting transduced Hepa1-6 cells into C57BL/6 mice to assess the effects of CDC25C knockdown on HCC development in *** proliferation and migration were evaluated using a Cell Counting Kit-8 cell proliferation assays and wound healing assays,*** expression of endoplasmic reticulum(ER)stress-related molecules(glucose-regulated protein 78,X-box binding protein-1,and C/EBP homologous protein)was measured in both cells and subcutaneous xenografts using quantitative real-time PCR(qRT-PCR)and western ***,apoptosis was investigated using flow cytometry,qRT-PCR,and western *** CDC25C was stably suppressed in Hepa1-6 and B16 cells through LV-CDC25C shRNA transduction.A xenograft model with CDC25C knockdown was successfully established and that downregulation of CDC25C expression significantly inhibited HCC growth in ***25C knockdown not only inhibited cell proliferation and migration but also significantly increased the ER stress response,ultimately promoting ER stress-induced apoptosis in HCC *** The regulatory mechanism of CDC25C in HCC development may involve the activation of ER stress and the ER stress-induced apoptosis signali

关键词： Cell division cyclin 25C Hepatocellular carcinoma Endoplasmic reticulum stress Proliferation Apoptosis

Dysprosium oxide,alumina,and graphene oxide reinforced polycaprolactone thin films:Thermal stability,morphology,and cell viability

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Journal of Rare Earths 2024年第7期42卷 1337-1343,I0005页

作者： Mohamed Tharwat Elabbasy Mohamed R.Atteya Mai A.Samak Mousa Abdelrahman Tahani AlMahmoud M.A.El-Morsy A.A.Menazea M.F.H.Abd El-Kader department of Public Health College of Public Health and Health InformaticsUniversity of Ha'ilHa'il2440Saudi Arabia Food Control department Faculty of Veterinary MedicineZagazig UniversityZagazig44519Egypt department of Physical Therapy College of Applied Medical SciencesUniversity of Ha'ilHa'ilSaudi Arabia department of Histology and Cell Biology Faculty of MedicineZagazig UniversityZagazig44519Egypt College of Nursing University of Ha'ilHa'ilSaudi Arabia College of Science and Humanities in Al-Kharj Physics DepartmentPlasma Technology and Material Science UnitPrince Sattam Bin Abdulaziz UniversityAl-kharj11942Saudi Arabia Physics department Faculty of ScienceUniversity of DamiettaNew Damietta34517Egypt Spectroscopy department Physics Research InstituteNational Research CentreDokki12622GizaEgypt Biophysics department Faculty of ScienceCairo UniversityGizaEgypt

Developing a biomaterial for wound healing applications is still a ***,dysprosium oxide(Dy_(2)O_(3)),aluminum oxide(Al_(2)O_(3)),and graphene oxide(GO)were embedded in cast films based on polycaprolactone(PCL)to be examined for wound dressing *** techniques were used to characterize the fabricated films including X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),Raman,and scanning electron microscopy(SEM),besides their biological *** thermogravimetric analysis(TGA)exhibits high thermal stability as the scaffold weight decreases slightly to 98.6%after raising the temperature from room temperature to 280℃.The cell viability was investigated and it is shown that the viable cells grow up to approximately 93%at 75μg/***,the cell attachment shows excellent behavior as the cells attach on most of the surface of the modified PCL which shows high biocompatibility.

关键词： Wound healing Polycaprolactone Dy_(2)O_(3) Al_(2)O_(3) Biocompatible Rare earths

Feeding behavior and hormoligosis associated with imidacloprid resistance in Asian citrus psyllid,Diaphorina citri

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Insect Science 2024年第4期31卷 1211-1221页

作者： Xue Dong Chen Justin George Lauren M.Diepenbrock Hunter Gossett Guoping Liu Jawwad A.Qureshi Lukasz L.Stelinski Entomology and Nematology department University of FloridaCitrus Research and Education CenterLake AlfredFloridaUSA Southern Insect Management research Unit USDA-ARSStonevilleMississippiUSA Entomology and Nematology department University of FloridaSouthwest Florida Research and Education CenterImmokaleeFloridaUSA

Imidacloprid is a neonicotinoid insecticide used for managing the Asian citrus psyllid,Diaphorina citri Kuwayama,which serves as vector of phytopathogens causing citrus ***,development of resistance to neonicotinoids among populations of *** has coincided with occasional control failures in the *** objectives of this research were to(1)survey current levels of imidacloprid resistance in Florida citrus;(2)compare feeding behavior between imidacloprid-resistant and susceptible *** using electrical penetration graph recordings,and(3)investigate the possible amplification of insecticide hormoligosis associated with *** surveys confirmed that the susceptibility of *** populations to imidacloprid has decreased in commercial Florida citrus groves compared with a laboratory-susceptible *** 12 generations of selection,resistance to imidacloprid increased by 438 fold compared with the susceptible ***-susceptible *** feeding on citrus exhibited significantly more bouts associated with intercellular pathway(C),phloem penetration(D),phloem salivation(E1),and nonprobing(Np)activities than imidacloprid-resistant ***,there were no differences observed in the frequency or duration of phloem ingestion or xylem feeding between susceptible and resistant *** was no statistical difference in fecundity between resistant and susceptible ***,the fecundity of imidacloprid-susceptible female *** treated with a sublethal concentration of imidacloprid(LC_(25))increased significantly compared with controls,while such hormoligosis was less pronounced among imidacloprid-resistant *** results suggest that imidacloprid-resistant psyllids may cease feeding sooner than susceptible counterparts following sublethal exposure to this insecticide,indicative of a behavioral resistance mechanism.

关键词： artificial selection electrical penetration graph feeding behavior hormoligosis insecticide resistance sublethal exposure