检索结果-南通市图书馆

Cellular & Molecular Immunology 2023年第8期20卷 972-975页

作者： Peter Dimitrion Iltefat Hamzavi Congcong Yin Jugmohit Toor Kalpana Subedi Namir Khalasawi Angela Miller Richard Huggins Indra Adrianto Jesse Veenstra Gautham Vellaichamy Aakash Hans Steven Daveluy Mohammad Athar Wilson Liao Henry Lim David Ozog Li Zhou Qing-Sheng Mi Center for Cutaneous Biology and Immunology Research Department of DermatologyHenry Ford Health SystemDetroitMI48202USA Immunology Research Program Henry Ford Cancer InstituteHenry Ford Health SystemDetroitMI48202USA Cancer Biology Graduate Program School of MedicineWayne State UniversityDetroitMI48202USA Center for Bioinformatics Department of Public Health SciencesHenry Ford Health SystemDetroitMI48202USA department of Dermatology Wayne State University School of MedicineDetroitMI48202USA University of Alabama at Birmingham(UAB)Research Center of Excellence in Arsenicals Department of DermatologyUniversity of Alabama at BirminghamBirminghamALUSA department of Dermatology University of California San FranciscoSan FranciscoCAUSA department of Biochemistry Microbiologyand ImmunologySchool of MedicineWayne State UniversityDetroitMI48202USA

Hidradenitis suppurativa(HS)is a chronic inflammatory skin disease with a worldwide prevalence of 0.3–4.0%,wherein abscesses and dermal sinus tracts form in intertriginous skin[1].HS remains poorly treated due to a l... 详细信息

关键词： CD38 patients pathogenesis

The Challenges of Diagnosis and Control of Enterotoxaemia Caused by <i>Clostridium perfringens</i>in Small Ruminants

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in microbiology 2020年第5期10卷 238-273页

作者： Rajveer Singh Pawaiya Kumaresan Gururaj Neeraj Kumar Gangwar Desh Deepak Singh Rahul Kumar Ashok Kumar Division of Animal Health ICAR-Central Institute for Research on Goats Mathura India department of veterinary Pathology College of Veterinary Science & Animal Husbandry DUVASU Mathura India department of veterinary Pathology College of Veterinary Science & Animal Husbandry NDAUT Kumarganj Faizabad India

Enterotoxaemia is one of the important pathologies caused by Clostridium perfringens, which produces intestinal and systemic disease in goats, sheep and other animals. These Gram-positive anaerobic bacteria are normally resident in the intestinal tract of ruminants but during favourable conditions, proliferate uncontrollably and release toxins which produce disease in the host. Different strains of C. perfringens are responsible for several clinical syndromes, including lamb dysentery, pulpy kidney disease and struck. However, the pathology and pathogenesis of caprine enterotoxaemia is not well understood, with limited studies available in goats. Caprine enterotoxaemia can be controlled with the better understanding of its risk factors and pathogenesis. The diagnosis of enterotoxaemia in animals is complex and often requires group of tests than one single test for better specificity and sensitivity. Tentative diagnosis of enterotoxaemia in sheep and goats is based on the history, clinical signs and gross lesions during post-mortem examination of animals;however, confirmatory diagnosis of enterotoxaemia requires different laboratory diagnostic tools. Toxin detection of C. perfringens in case of enterotoxaemia is furthermost accepted benchmark in establishing a definitive diagnosis of enterotoxaemia in intestinal contents. Measuring urine glucose or observing Gram-stained smears of intestinal mucosa can be used as supplementary tests. However, it is also imperative that enterotoxaemia cannot be ruled out in the event of negativity of aforementioned diagnostic tests. Hence, definitive diagnosis of enterotoxaemia in goats can be achieved with the use of molecular techniques (PCR, ELISA and immune-fluorescence) coupled with toxin detection in intestine or biological assays including mouse inoculation test (MIT). In case of goats, vaccine efficacy is poor which may be due to need of high to moderate level of serum antibodies to protect against both systemic and enteric ef

关键词： Clostridium perfringens Control Diagnosis Enterotoxaemia Goat Prevention Sheep Small Ruminants

Identification of two migratory colon ILC2 populations differentially expressing IL-17A and IL-5/IL-13

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2023年第1期66卷 67-80页

作者： Hongzhi Liu Liang Li Yanyun Hao Jialu Li Zhaoyuan Liu Jingjing Qi Jingjing Zhang Ningbo Wu Dandan Wu Caixia Gao Lei Chen Lei Shen Jinke Cheng Bing Su Shanghai Institute of Immunology Department of Immunology and Microbiologythe Ministry of Education Key Laboratory of Cell Death and DifferentiationShanghai Jiao Tong University School of MedicineShanghai 200025China department of Biochemistry and Molecular Cell Biology Shanghai Key Laboratory for Tumor Microenvironment and InflammationShanghai Jiao Tong University School of MedicineShanghai 200025China State Key Laboratory for Oncogenes and Related Genes Key Laboratory of Gastroenterology and HepatologyMinistry of HealthDivision of Gastroenterology and HepatologyShanghai Institute of Digestive DiseaseRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai Cancer InstituteShanghai Institute of Digestive DiseaseShanghai 200001China department of Biliary and Pancreatic Surgery Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

Group 2 innate lymphoid cells(ILC2s)play important tissue resident roles in anti-parasite immunity,allergic immune response,tissue homeostasis,and tumor ***2s are considered tissue resident cells with little proliferation at steady *** studies have shown that a subset of small intestinal ILC2s could leave their residing tissues,circulate and migrate to different organs,including lung,liver,mesenteric LN and spleen,upon ***,it remains unknown whether other ILC populations with migratory behavior *** this study,we find two major colon ILC2 populations with potential to migrate to the lung in response to IL-25 *** subset expresses IL-17A and resembles inflammatory ILC2s(iILC2s)but lacks CD27 expression,whereas the other expresses CD27 but not *** addition,the IL-17A^(+)ILC2s express lower levels of CD127,CD25,and ST2 than CD27^(+)ILC2s,which express higher levels of IL-5 and ***,we found that both colon ILC2 populations still maintained their colonic features of preferential expression of IL-17A and CD27,IL-5/IL-13,***,our study identifies two migratory colon ILC2 subsets with unique surface markers and cytokine profiles which are critical in regulating lung and colon immunity and homeostasis.

关键词： colon ILC2s IL-17A CD27 iILC2 lung inflammation

Mesenchymal stem cells transplantation attenuates experimentally induced brain injury after neonatal hypoxia by different two routes of administrations

在线全文

学校读者我要写书评

暂无评论

BIOCELL 2019年第1期43卷 21-28页

作者： Nesrine EBRAHIM Eman EHSAN Eman Abd EL GHANY Dina SABRY Ashraf SHAMAA Histology and Cell Biology department Faculty of MedicineBenha UniversityBenhaEgypt Pediatrics department Faculty of MedicineCairo UniversityGizaEgypt Medical Biochemistry and molecular biology department Faculty of MedicineCairo UniversityGizaEgypt Surgical and Radiology department Faculty of Veterinary MedicineCairo UniversityGizaEgypt

The neonatal hypoxic-ischemic encephalopathy(HIE)is an important cause of neurological morbidity and mortality in *** therapy is considered a promising method for treating severe neurological disorders such as this *** cells have the capacity for self-renewal and differentiation into certain cell *** present study was aimed to find out the most beneficial route of bone marrow-derived mesenchymal stem cells(BMSCs)administration for the attenuation of experimentally induced HIE in neonatal *** neonatal rats were divided randomly into four *** 1:control *** 2:rats were exposed to bilateral ligation of cephalic *** 3:rats were exposed to bilateral ligation of cephalic arteries and then underwent intravenous(IV)BMSC *** 4:rats were exposed to bilateral ligation of cephalic arteries and then underwent intracerebroventricular(ICV)BMSC *** animals were evaluated by(a)neurobehavioral tests;(b)histopathology,i.e.,histological and immuno-histochemical studies;and(3)gene expression *** BMSC treated groups(3 and 4)showed improvement in neurobehavioral tests,histopathological studies,and gene expression,as compared to non-injected lesioned rats(Group 2)with better improvement in Group 4(ICV injections)than in Group 3(IV injections).

关键词： Cerebral cortex Neonatal hypoxic ischemia Stem cells

Serum omentin and vaspin levels in cirrhotic patients with and without portal vein thrombosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第14期23卷 2613-2624页

作者： Michal Kukla Marek Waluga Michal Zorniak Agnieszka Berdowska Piotr Wosiewicz Tomasz Sawczyn Rafal J Buldak Marek Ochman Katarzyna Ziora Tadeusz Krzemiński Marek Hartleb department of Gastroenterology and Hepatology Medical University of Silesia in Katowice department of microbiology and Biotechnology Jan Dlugosz University department of Physiology Medical University of Silesia in Katowice department of Biochemistry in Zabrze Medical University of Silesia in Katowice department of Cardiac Surgery and Transplantation Silesian Center for Heart Diseases department of Pediatrics Medical University of Silesia in Katowice department of Pharmacology Medical University of Silesia in Katowice

AIM To investigate serum omentin and vaspin levels in cirrhotic patients; and to assess the relationship of these levels with hemostatic parameters, metabolic abnormalities, cirrhosis severity and *** Fifty-one cirrhotic patients(17 with portal vein thrombosis) were analyzed. Serum omentin and vaspin levels were measured with commercially available direct enzyme-linked immunosorbent assays(ELISAs). To assess platelet activity, the following tests were performed using a MULTIPLATE?PLATELET FUNCTION ANALYZER:(1) an ADP-induced platelet activation test;(2) a cyclooxygenase dependent aggregation test(ASPI test);(3) a von Willebrand factor and glycoprotein Ibdependent aggregation(using ristocetin) test(RISTO test); and(4) a test for thrombin receptor-activating peptide-6 induced activation of the thrombin receptor, which is sensitive to Ⅱb/Ⅲa receptor antagonists. RESULTS Omentin, but not vaspin, serum concentrations were significantly decreased in patients with portal vein thrombosis(PVT)(P = 0.01). Prothrombin levels were significantly increased in patients with PVT(P = 0.01). The thrombin receptor activating peptide(TRAP) test results were significantly lower in the PVT group(P = 0.03). No significant differences in adipokines serum levels were found regarding the etiology or severity of liver cirrhosis assessed according to the Child-Pugh or Model of End-Stage Liver Disease(MELD) scores. There was a significant increase in the TRAP(P = 0.03), ASPI(P = 0.001) and RISTO high-test(P = 0.02) results in patients with lower MELD scores. Serum omentin and vaspin levels were significantly down-regulated in patients without insulin resistance(P = 0.03, P = 0.02, respectively). A positive relationship between omentin and vaspin levels were found both when all of the patients were analyzed(r = 0.41, P = 0.01) and among those with PVT(r = 0.94, P < 0.001).CONCLUSION Serum omentin levels are increased in patients without PVT. Cirrhosis origin and grade do not affect

关键词： Omentin Vaspin 肝硬化 Adipokine 门静脉血栓门高血压

Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2006年第5期8卷 589-594页

作者： Liang Chen Zhong-Cheng Xin Xin Li Long Tian Yi-Ming Yuan Gang Liu Xue-Jun Jiang Ying-Lu Guo Andrology Center of Peking University First Hospital Beijing 100009 China department of Surgery People＇s Hospital of Zhangqiu Zhangqiu 250200 China Institute of microbiology Chinese Academy of Sciences Beijing 100080 China

Aim： To investigate the regulatory function of Cox7a2 on steroidogenesis and the mechanism involved in TM3 mouse Leydig cells. Methods： The cDNA of Cox7a2 was cloned from TM3 mouse Leydig cells. It was subcloned to pDsRed- Express-N 1 and transfected back into TM3 mouse Leydig cells for Cox7a2 overexpression by transient gene transfection. Steroidogenesis affected by overexpressed Cox7a2 was studied by ELISA. To elicit the mechanism of this effect, expression of steroidogenic acute regulatory （STAR） protein and reactive oxygen species （ROS） were examined by Western blot and fluorometer, respectively. Results： The cDNA of Cox7a2 （249 bp） was cloned from Leydig cells and confirmed by DNA sequencing. After constructed pDsRed-Express-N1-Cox7a2 was transfected back into TM3 mouse Leydig cells, Cox7a2 inhibited not only luteinizing hormone （LH）-induced secretion of testosterone but also the expression of StAR protein. At the same time, Cox7a2 increased the activity of ROS in TM3 mouse Leydig cells. Conclusion： Cox7a2 inhibited LH-induced StAR protein expression, and consequent testosterone production, at least in part, by increasing ROS activity in TM3 mouse Leydig cells.

关键词： Cox7a2 fusion protein steroidogenesis steroidogenic acute regulatory protein reactive oxygen species Leydig cell

Oral polymorphonuclear neutrophil characteristics in relation to oral health： a cross-sectional, observational clinical study

在线全文

学校读者我要写书评

暂无评论

International Journal of Oral Science 2016年第3期8卷 191-198页

作者： Patrick Rijkschroeff Ineke DC Jansen Fridus A van der Weijden Bart IF Keijser Bruno G Loos Elena A Nicu department of Periodontology Academic Centre for Dentistry Amsterdam Amsterdam The Netherlands department of Preventive Dentistry Academic Centre for Dentistry Amsterdam Amsterdam The Netherlands microbiology and Systems Biology TNO Earth Environmental and Life Sciences Zeist The Netherlands Top Institute Food and Nutrition Wageningen The Netherlands

Polymorphonuclear neutmphils （PMNs） have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs （oPMNs） compared with circulatory PMNs （cPMNs）. Oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. PMN counts, cell cycle analysis and cellular activation state were investigated. Also, reactive oxygen species （ROS） production was analyzed, with and without bacterial stimulation （Fusobacterium nucleatum）. In males, 1.2 × 10^6- 1.0 ×10^6 oPMNs were collected, and showed a tendency to correlate with the levels of gingival bleeding （r= 0.215, P= 0.008）. Comparable oPMNs counts were found among females （1.0 ×10^6＋ 0.7 × 10^6）. More late-stage apoptotic/necrotic cells were found among the oPMNs （53.1%） compared with the cPMNs （8.5%; P〈 0.001）. Without additional stimulation, oPMNs were more activated than cPMNs, as indicated by higher expression of CD11b, CD63 and CD66b, and higher constitutive ROS levels （P〈 0.001）. Notably, in response to bacterial stimulation, oPMNs released comparable ROS levels as cPMNs （P= 0.042）. In conclusion, this study provides data on viable oPMNs showing high levels of activation in orally and systemically healthy individuals, free of apparent caries lesions and periodontal disease. These data suggests that although the oPMNs are in a more mature stage of their life cycle compared with the cPMNs, oPMNs are still responsive to stimulation, which indicates their functional potential and possible contribution to a healthy oral ecosystem.

关键词： apoptosis degranulation neutrophils oral health reactive oxygen species

Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2021年第4期10卷 452-459页

作者： M.Subbulakshmi Sugapriya Dhanasekaran S.Abirami M.Kannan R.Palaniappan Divya Venugopal department of microbiology K.R.College of Arts and ScienceKovilpattiTamil NaduIndia department of Medical Laboratory Sciences College of Applied Medical SciencesPrince Sattam Bin Abdulaziz UniversityWadi Al DawaserSaudi Arabia department of microbiology Kamaraj CollegeThoothukudiTamil NaduIndia Research department of Zoology V.H.N.Senthikumara Nadar College(Autonomus)VirudhunagarTamil NaduIndia Research department of Computer Science V.H.N.Senthikumara Nadar College(Autonomus)VirudhunagarTamil NaduIndia

Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative *** study identifi ed a unique isoform of 18S rRNA gene(864 bp)from a novel wild mushroom(SMK-1)(GenBank accession number:SUB3267363).Thin layer chromatographic(TLC)profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and fl avonoid fractions with retention factor(Rf)values of 0.955 and 0.927 *** GC/MS chromatograms of the SMK-1 methanolic extract identifi ed the main bioactive compound was phenol,5-methyl-2-(1-methylethyl)(74.00%)(thymol).The radical scavenging activity for the fl avonoid fraction was greater than the phenolic fractions(Rf–phenolics fractions>Rf–fl avonoid fractions)with the antioxidant activity more than that of standard ascorbic ***,the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5μg/mL to 80μg/mL in a dose-dependent *** present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefi *** application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug.

关键词： Antioxidant Anti-proliferation Dietary mushrooms HeLa cells Novel Mushrooms Thymol

Programmed death 1 (PD-1) and ligand (PD-L1) inhibitors in head and neck squamous cell carcinoma: A meta-analysis

在线全文

学校读者我要写书评

暂无评论

World Journal of Otorhinolaryngology-Head and Neck Surgery 2022年第3期8卷 177-186页

作者： Dylan A.Levy Jaimin J.Patel Shaun A.Nguyen W.Nicholas Jungbauer David M.Neskey Ezra E.W.Cohen Chrystal M.Paulos John A.Kaczmar Hannah M.Knochelmann Terry A.Day department of Otolaryngology-Head and Neck Surgery Medical University of South CarolinaCharlestonSouth CarolinaUSA department of Cell and Molecular Pharmacology and Developmental Therapeutics Medical University of South CarolinaCharlestonSouth CarolinaUSA department of Medicine Division of Hematology-OncologyUniversity of CaliforniaSan DiegoLa JollaCaliforniaUSA department of microbiology and Immunology Medical University of South CarolinaCharlestonSouth CarolinaUSA Sdepartment of Dermatology and Dermatologic Surgery Medical University of South CarolinaCharlestonSouth CarolinaUSA Division of Hematology&Oncology Medical University of South CarolinaCharlestonSouth CarolinaUsA

Background: PD-1 and PD-L1 inhibitors have emerged as promising treatments for patients with head and neck squamous cell carcinoma (HNSCC).Methods: Systematic review and meta-analysis of PD-1 and PD-L1 inhibitors in HNSCC. Outcomes: median overall survival (mOS), median progression-free survival (mPFS), Response Evaluation Criteria in Solid Tumors (RECIST) and treatment-related adverse events (TRAEs).Results: Eleven trials reported data on 1088 patients (mean age: 59.9 years, range: 18-90). The total mOS was 7.97 months (range: 6.0-16.5). Mean mPFS for all studies was 2.84 months (range: 1.9-6.5). PD-1 inhibitors had a lower rate of RECIST Progressive Disease than PD-L1 inhibitors (42.61%, 95% confidence interval [CI]: 36.29-49.06 vs. 56.79%, 95% CI: 49.18-64.19,P < 0.001). The rate of TRAEs of any grade (62.7%, 95% CI: 59.8-65.6) did not ***: Meta-analysis shows the efficacy of PD-1 and PD-L1 inhibitors in HNSCC and suggests a possible difference in certain RECIST criterion between PD-1 and PD-L1 inhibitors. Future work to investigate the clinical significance of these findings is warranted.

关键词： antibodies disease progression head and neck neoplasms humanized meta-analysis monoclonal squamous cell carcinoma of the neck

Isolation and Growth Characteristics of SARS-CoV-2 in Vero Cell

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2020年第3期35卷 348-350页

作者： Pingping Yao Yachun Zhang Yisheng Sun Yulin Gu Fang Xu Bo Su Chen Chen Hangjing Lu Dehui Wang Zhangnv Yang Biao Niu Jiancai Chen Lixia Xie Lei Chen Yajing Zhang Hui Wang Yuying Zhao Yue Guo Juncheng Ruan Zhiyong Zhu Zhenfang Fu Dayong Tian Qi An Jianmin Jiang Hanping Zhu Key Lab of Vaccine Prevention and Control of Infectious Disease of Zhejiang ProvinceZhejiang Provincial Center for Disease Control and PreventionHangzhou 310051China R&D department Shanghai King-Cell Biotechnology Co.Ltd.Shanghai 201506China State Key Laboratory of Agricultural microbiology Huazhong Agricultural UniversityWuhan 430070China College of veterinary Medicine Huazhong Agricultural UniversityWuhan 430070China

Dear Editors,The coronavirus disease 2019(COVID-19),caused by SARS-CoV-2,broke out in early December 2019 has escalated into a global pandemic(Lai et al.2020).Till the May 20 th 2020,more than 4,700,000 people were in... 详细信息

关键词： al. infected Cell