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A comparative study on the solid phase capture--platelet test, platelet adhesion immunofluorescence test and lymphocytotoxic test

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中华医学杂志(英文版) 1993年第7期106卷 537-537页

作者： Jin Jie Lin Xiu-ji R. Dorner V. Sachs department of Hematology First Affiliated Hospital Zhejiang Medical University Hangzhou 310003 department of tranfusion medicine and immunochematology University of Kiel Germany

The sensitivity, specificity and reproducibility of the solid phase capture-platelet test (CPT) were compared with those of the platelet adhesion immunofluorescence test (PAIFT) and the lymphocytotoxic test (LCT). This study shows that CPT has a higher sensitivity than PAIFT (P<0.001) and LCT (P<0.01). The specificity was 86% lower than LCT (100%) (P<0.01). Both the CPT test and PAIFT were comparable with regard to reproducibility (100%, 92%). The solid phase assay is easier to handle than PAIFT and LCT, allowing identification of HLA as well as of platelet specific antibodies. It is an efficient antibody screening test.

关键词： ALLOANTIBODIES ANTIBODIES ELISA

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Deciphering transcriptome alterations in bone marrow hematopoiesis at single-cell resolution in immune thrombocytopenia

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Signal Transduction and Targeted Therapy 2022年第11期7卷 4190-4207页

作者： Yan Liu Xinyi Zuo Peng Chen Xiang Hu Zi Sheng Anli Liu Qiang Liu Shaoqiu Leng Xiaoyu Zhang Xin Li Limei Wang Qi Feng Chaoyang Li Ming Hou Chong Chu Shihui Ma Shuwen Wang Jun Peng department of Hematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinan250012China State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesHaihe Laboratory of Cell EcosystemInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin300020China department of Hematology the Affiliated Hospital of Qingdao UniversityQingdao266000China Advanced Medical Research Institute Shandong UniversityJinan250012China Shangdong Key Laboratory of immunochematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinan250012China Key Laboratory of Cardiovascular Remodeling and Function Research Chinese Ministry of Education and Chinese Ministry of HealthQilu HospitalCheeloo College of MedicineShandong UniversityJinan250012China department of Biomedical Informatics Harvard Medical SchoolBoston02115MAUSA

Immune thrombocytopenia(ITP)is an autoimmune disorder,in which megakaryocyte dysfunction caused by an autoimmune reaction can lead to thrombocytopenia,although the underlying mechanisms remain ***,we performed single-cell transcriptome profiling of bone marrow CD34+hematopoietic stem and progenitor cells(HSPCs)to determine defects in megakaryopoiesis in *** expression,cell-cell interactions,and transcriptional regulatory networks varied in HSPCs of ITP,particularly in immune cell *** expressed gene(DEG)analysis indicated that there was an impaired megakaryopoiesis of *** cytometry confirmed that the number of CD9+and HES1+cells from Lin−CD34+CD45RA−HSPCs decreased in *** culture assays demonstrated that CD9+Lin−CD34+CD45RA−HSPCs tended to differentiate into megakaryocytes;however,this tendency was not observed in ITP patients and more erythrocytes were *** percentage of megakaryocytes differentiated from CD9+Lin−CD34+CD45RA−HSPCs was 3-fold higher than that of the CD9−counterparts from healthy controls(HCs),whereas,in ITP patients,the percentage decreased to only 1/4th of that in the HCs and was comparable to that from the CD9−***,when co-cultured with pre-B cells from ITP patients,the differentiation of CD9+Lin−CD34+CD45RA−HSPCs toward the megakaryopoietic lineage was *** analysis revealed that megakaryocytic progenitors(MkP)can be divided into seven subclusters with different gene expression patterns and *** ITP-associated DEGs were MkP subtype-specific,with most DEGs concentrated in the subcluster possessing dual functions of immunomodulation and platelet *** study comprehensively dissects defective hematopoiesis and provides novel insights regarding the pathogenesis of ITP.

关键词： impaired alterations progenitor

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