检索结果-南通市图书馆

Effect of high hydrostatic pressure on prebiotic peptide synthesis

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2019年第2期30卷 367-370页

作者： Jianxi Ying Peng Chen Yile Wu Xu Yang Kaili Yan Pengxiang Xu Yufen Zhao department of Chemistry and Key Laboratory for Chemical Biology of Fujian Province College of Chemistry and Chemical EngineeringXiamen University Institute of drug discovery Technology Ningbo University Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education) Department of ChemistryTsinghua University

Prebiotic peptide synthesis is a central issue concerning life's origins. Many studies considered that life might come from Hadean deep-sea environment, that is, under high hydrostatic pressure ***, the properties of prebiotic peptide formation under high hydrostatic pressure conditions have seldom been mentioned. Here we report that the yields of dipeptides increase with raised ***, effect of pressure on the formation of dipeptide was obvious at relatively low *** that the deep sea is of high hydrostatic pressure, the pressure may serve as one of the key factors in prebiotic peptide synthesis in the Hadean deep-sea environment. The high hydrostatic pressure should be considered as one of the significant factors in studying the origin of life.

关键词： High hydrostatic pressure Sodium trimetaphosphate Prebiotic peptide synthesis Prebiotic chemistry Origin of life

Bulbine frutescens phytochemicals as novel ABC-transporter inhibitor:a molecular docking and molecular dynamics simulation study

在线全文

学校读者我要写书评

暂无评论

Journal of Cancer Metastasis and Treatment 2021年第1期7卷 20-32页

作者： Prem Prakash Kushwaha Santosh Kumar Maurya Amit Singh Kumari Sunita Prajapati Atul Kumar Singh Mohd Shuaib Shashank Kumar Molecular Signaling&drug discovery Laboratory Department of BiochemistryCentral University of PunjabBathinda 151401India department of Computational Sciences Central University of PunjabBathinda 151401India.

Aim:The present in silico study aimed to evaluate the ATP-binding cassette(ABC)transporter inhibition potential of Bulbine frutescens(***)***:Several previous studies and databases were used to retrieve the ligands and target protein *** molecular docking study was performed using the Auto Dock Tools,and the GROMACS package was applied to accomplish molecular dynamics ***:Utilizing the molecular docking and simulation approach,~25 phytochemicals were screened against the ABC transporter *** score analysis revealed that *** phytochemical 4’-Demethylknipholone 2’-β-D-glucopyranoside exhibited strong binding on the ABC transporter protein with a minimum binding score-9.8 kcal/mol in comparison to the standard ABC transporter inhibitor diltiazem(-6.86 kcal/mol).Furthermore,molecular dynamics simulation for 4’-Demethylknipholone 2’-β-D-glucopyranoside showed an acceptable root mean square deviation,radius of gyration,root mean square fluctuation,and hydrogen bond,in addition to other lead ***:The in-silico study demonstrated that *** phytochemical 4’-Demethylknipholone 2’-β-D-glucopyranoside possesses anti-drug resistance properties and requires further testing in preclinical settings.

关键词： Cancer drug resistance ABC-transporter natural inhibitors Bulbine frutescens MM-GBSA

Using DelPhi Capabilities to Mimic Protein’s Conformational Reorganization with Amino Acid Specific Dielectric Constants

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Communications in Computational Physics 2013年第1期13卷 13-30页

作者： Lin Wang Zhe Zhang Walter Rocchia Emil Alexov Computational Biophysics and Bioinformatics Department of PhysicsClemson UniversityClemsonSC 29634USA drug discovery and Development Italian Institute of Technologyvia Morego 3016163 GenovaItaly

Many molecular events are associated with small or large conformational changes occurring in the corresponding *** such changes is a challenge and requires significant amount of computing *** point of view of electrostatics,these changes can be viewed as a reorganization of local charges and dipoles in response to the changes of the electrostatic field,if the cause is insertion or deletion of a charged amino *** we report a large scale investigation of modeling the changes of the folding energy due to single mutations involving charged *** allows the changes of the folding energy to be considered mostly electrostatics in origin and to be calculated with DelPhi assigning residue-specific value of the internal dielectric constant of *** predicted energy changes are benchmarked against experimentally measured changes of the folding energy on a set of 257 single *** best fit between experimental values and predicted changes is used to find out the effective value of the internal dielectric constant for each type of amino *** predicted folding free energy changes with the optimal,amino acid specific,dielectric constants are within RMSD=0.86 kcal/mol from experimentally measured changes.

关键词： DelPhi protein electrostatics dielectric constant Poisson-Boltzmann equation protein flexibility energy calculations single point mutations

Use of polymer combinations in the preparation of solid dispersions of a thermally unstable drug by hot-melt extrusion

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2013年第4期3卷 263-272页

作者： Jia Liu Feng Cao Can Zhang Qineng Ping department of Pharmaceutics School of PharmacyChina Pharmaceutical UniversityNanjing 210009China Center of drug discovery State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjing 210009China

The objective of the study was to prepare solid dispersions containing a thermally unstable drug by hot-melt extrusion(HME).Carbamazepine(CBZ)was selected as model drug and combinations of Kollidon VA64(VA64),Soluplus(SOL)and Eudragit EPO(EPO)were utilized as *** was conducted to identify the suitability of polymer combinations based on solubility parameters,differential scanning calorimetry(DSC),hot stage microscopy and thermogravimetric *** properties of solid dispersions were determined by DSC,X-ray diffraction,fourier transform infrared spectroscopy,dissolution and accelerated stability *** results show that drug-polymer miscibility at temperatures below the melting point(Tm)of CBZ was improved by combining EPO with VA64 or *** 30%drug loading in a solid dispersion in SOL:EPO(1:1,w/w),CBZ was mainly present in an amorphous form accompanied by a small amount of a microcrystalline *** dissolution rate of the solid dispersion was significantly increased(approximately 90%within 5 min)compared to either the pure drug(approximately 85%within 60 min)or the corresponding physical mixture(approximately 80%within 60 min)before and after *** solid dispersion in SOL:EPO(1:1,w/w)was relatively stable at 401C/75%RH under CBZ tablet packaging conditions for at least 3 *** conclusion,polymer combinations that improve drug-polymer miscibility at an HME processing temperature below the Tm of a drug appear to be beneficial in the preparation of solid dispersions containing thermally unstable drugs.

关键词： Carbamazepine Hot-melt extrusion Thermal unstable drug Solid dispersion Polymer combination Stability Dissolution

Mechanism underlying Fanmugua(Fructus Caricae)leaf multicomponent synergistic therapy for anemia:data mining based on hematopoietic network

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medicine 2023年第3期43卷 542-551页

作者： JIA Lihong TIE Defu FAN Zhaohui CHEN Dan CHEN Qizhu CHEN Jun BO Huaben School of bioscience and Biopharmaceutics Guangdong Province Key Laboratory for Biotechnology Drug CandidatesGuangdong Pharmaceutical UniversityGuangdong 510006China department of Pharmacy Zhanjiang Health School of GuangdongGuangdong 524037China College of Pharmacy Guangdong Pharmaceutical UniversityGuangdong 510006China

OBJECTIVE:To investigate the underlying mechanism of Fanmugua(Fructus Caricae) Leaf(CPL) multicomponent synergistic therapy for ***:The components were identified in the *** databases were searched for targets of *** analysis was used to determine the targets associated with anemia and in bone *** on the Kyoto Encyclopedia of Genes and Genomes database,pathways and targets related to hematopoiesis were *** key targets were obtained by protein-protein interaction *** docking was used to analyze the binding ability of key targets and active *** marrow cells were used as an experimental model to verify the drug ***:A total of 139 components and 1868 targets of CPL were retrieved from the *** disease enrichment analysis,543 targets for hemorrhagic anemia,223 targets for aplastic anemia,and 126 targets for sickle cell anemia were *** organ enrichment yielded 27,29,and 20 targets of bone *** on KEGG pathway enrichment,a total of 47 shared hematopoietic pathways and 42 related targets were *** key targets were vascular endothelial growth factor A(VEGFA),interleukin 10(IL-10),plateletendothelial cell adhesion molecule-1(PECAM1),C-C motif chemokine 2(CCL2),and vascular cell adhesion molecule 1(VCAM1).The CPL active components included ursolic acid,quercetin,and *** expression of VEGFA was significantly increased after CPL *** and ursolic acid acted on *** and Hesperidin acted on *** acted on IL-10,CCL2,VCAM1,and *** experiments revealed that CPL could promote the proliferation and migration of bone marrow ***:CPL has the synergistic efficacy of treating anemia through multiple components,targets,and pathways.

关键词： anemia data mining network pharmacology molecular docking simulation Carica papaya(Fructus Caricae)Leaf synergistic effect

Inhibition of cerebral ischemia/reperfusion injuryinduced apoptosis:nicotiflorin and JAK2/STAT3 pathway

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2017年第1期12卷 96-102页

作者： Guang-qiang Hu Xi Du Yong-jie Li Xiao-qing Gao Bi-qiong Chen Lu Yu department of Anatomy Southwest Medical UniversityLuzhouSichuan ProvinceChina department of Chemistry Southwest Medical UniversityLuzhouSichuan ProvinceChina drug discovery Research Center Southwest Medical UniversityLuzhouSichuan ProvinceChina department of Anatomy and Neurobiology Southwest Medical UniversityLuzhouSichuan ProvinceChina

Nicotiflorin is a flavonoid extracted from Carthamus *** studies have shown its cerebral protective effect,but the mechanism is *** this study,we aimed to determine whether nicotiflorin protects against cerebral ischemia/reperfusion injury-induced apoptosis through the JAK2/STAT3 *** cerebral ischemia/reperfusion injury model was established by middle cerebral artery occlusion/***（10 mg/kg） was administered by tail vein *** apoptosis in the ischemic cerebral cortex was examined by hematoxylin-eosin staining and terminal deoxynucleotidyl transferase d UTP nick end labeling ***-2 and Bax expression levels in ischemic cerebral cortex were examined by immunohistochemial ***,p-JAK2,p-STAT3,Bcl-2,Bax,and caspase-3 levels in ischemic cerebral cortex were examined by western blot *** altered the shape and structure of injured neurons,decreased the number of apoptotic cells,down-regulates expression of p-JAK2,p-STAT3,caspase-3,and Bax,decreased Bax immunoredactivity,and increased Bcl-2 protein expression and *** results suggest that nicotiflorin protects against cerebral ischemia/reperfusion injury-induced apoptosis via the JAK2/STAT3 pathway.

关键词： nerve regeneration brain injury nicotiflorin ischemic stroke cerebral ischemia/reperfusion injury treatment cell apoptosis terminal deoxynucleotidyl transferase dUTP nick end labeling JAK2/STAT3 pathway Bcl-2 Bax caspase-3 neural regeneration

Synthesis and evaluation of 2-cyano-3, 12-dioxooleana-1, 9(11)-en-28-oate-13β, 28-olide as a potent anti-inflammatory agent for intervention of LPS-induced acute lung injury

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2017年第5期15卷 347-354页

作者： MOU Yi JIAN Yan-Lin CHEN Tong HUANG Zhang-Jian QIAO Yi-Xue PENG Si-Xun ZHANG Da-Yong JI Hui ZHANG Yi-Hua State Key Laboratory of Natural Medicines Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases China Pharmaceutical University Nanjing 210009 China State Key Laboratory of Natural Medicines Department of Pharmacology China Pharmaceutical University Nanjing 210009 China

The present study was designed to synthesize 2-Cyano-3, 12-dioxooleana-1, 9(11)-en-28-oate-13β, 28-olide(1), a lactone derivative of oleanolic acid(OA) and evaluate its anti-inflammatory activity. Compound 1 significantly diminished nitric oxide(NO) production and down-regulated the m RNA expression of iNOS, COX-2, IL-6, IL-1β, and TNF-α in lipopolysaccharide(LPS)-stimulated RAW264.7 cells. Further in vivo studies in murine model of LPS-induced acute lung injury(ALI) showed that 1 possessed more potent protective effects than the well-known anti-inflammatory drug dexamethasone by inhibiting myeloperoxidase(MPO) activity, reducing total cells and neutrophils, and suppressing inflammatory cytokines expression, and thus ameliorating the histopathological conditions of the injured lung tissue. In conclusion, compound 1 could be developed as a promising anti-inflammatory agent for intervention of LPS-induced ALI.

关键词： Oleanolic acid Lactone Anti-inflammation Acute lung injury

Asponchimides A−E: new enantiomeric N-acetyldopamine trimers from Aspongopus chinensis

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2023年第11期21卷 859-867页

作者： NIE Jinchun MEI Fang ZHENG Yueyuan WEN Qiuyi LI Zhenwei ZHANG Daidi LI Wei GUO Dean School of Pharmaceutical Sciences Southern Medical UniversityGuangzhou 510515China Zhongshan Institute for drug discovery Shanghai Institute of Materia MedicaChinese Academy of SciencesZhongshan 528400China

Five new racemic N-acetyldopamine (NADA) trimers, asponchimides A−E ( 1 − 5 ), were isolated from Aspongopus chinensis, a prominent traditional Chinese medicinal insect employed for alleviating pain, treating indigestion, and addressing kidney ailments. Compounds 1 − 5 were successfully resolved by chiral high-performance liquid chromatography (HPLC), yielding five pairs of enantiomers: (+)- and (−)-asponchimides A−E ( 1a / 1b − 5a / 5b ). Their structural identities were discerned by extensive spectroscopic analyses, including high-resolution mass spectrometry (HRMS), ultraviolet-visible (UV-Vis) spectroscopy, infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR), and their absolute configurations were determined by electronic circular dichroism (ECD) calculations. Compounds 1 − 5 are pioneering instances of NADA trimers featuring a Δ7 double bond. When subjected to a series of bioassays, a majority of the compounds exhibited weak inhibitory activity against nitric oxide (NO) production in LPS-induced RAW 264.7 cells.

关键词： Aspongopus chinensis N-acetyldopamine trimer ECD calculations NO inhibitory activity

Tripterygium wilfordii multiglycoside-induced hepatotoxicity via inflammation and apoptosis in zebrafish

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2021年第10期19卷 750-757页

作者： DUAN Xiu-Ying MA Rui-Jiao HSIAO Chung-Der JIANG Zhen-Zhou ZHANG Lu-Yong ZHANG Yun LIU Ke-Chun Biology Institute Qilu University of Technology(Shandong Academy of Sciences)Jinan 250103China Engineering Research Center of Zebrafish Models for Human Diseases and drug Screening of Shandong Province Jinan 250103China department of bioscience Technology Chung Yuan Christian UniversityTaoyuan 32023China Jiangsu Key Laboratory of drug Screening China Pharmaceutical UniversityNanjing 210009China

Tripterygium wilfordii multiglycoside(GTW)is a commonly used compound for the treatment of rheumatoid arthritis(RA)and immune diseases in clinical ***,it can induce liver injury and the mechanism of hepatotoxicity is still not *** study was designed to investigate GTW-induced hepatotoxicity in zebrafish larvae and explore the mechanism *** 72 hpf(hours post fertilization)zebrafish larvae were administered with different concentrations of GTW for three days and their mortality,malformation rate,morphological changes in the liver,transaminase levels,and histopathological changes in the liver of zebrafish larvae were *** reverse transcription-polymerase chain reaction(RT-PCR)was used to examine the levels of microRNA-122(miR-122)and genes related to inflammation,apoptosis,cell proliferation and liver *** results showed that GTW increased the mortality of zebrafish larvae,while significant malformations and liver damage *** main manifestations were elevated levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),significant liver atrophy,vacuoles in liver tissue,sparse cytoplasm,and unclear hepatocyte ***-PCR results showed that the expression of miR-122 significantly decreased by GTW;the mRNA levels of inflammation-related genes il1β,il6,tnfα,il10,cox2 and ptges significantly increased;the mRNA level of tgfβsignificantly decreased;the mRNA levels of apoptosis-related genes,caspase-8 and caspase-9,significantly increased;the mRNA level of bcl2 significantly decreased;the mRNA levels of cell proliferation-related genes,top2αand uhrf1,significantly reduced;the mRNA levels of liver function-related genes,alr and cyp3c1,significantly increased;and the mRNA level of cyp3a65 significantly *** zebrafish,GTW can cause increased inflammation,enhanced apoptosis,decreased cell proliferation,and abnormal expression of liver function-related genes,leading to abnormal liver structure and funct

关键词： Tripterygium wilfordii multiglycoside Zebrafish Hepatotoxicity Inflammation Apoptosis