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Modified Method for Showing Gc Subtypes and the Discovery of Two New Variants of Gc Subtypes from Chinese

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中国医科大学学报 1991年第S2期20卷 29-32页

作者：王魏刘玉华贾静涛 department of Forensic serology Faculty of Forensic Medicine China Medical University Shenyang China

An improved method for showing Gc band by sulfosalicylic acidafter IEF is reported. This method provides us an effective mean to identify theGc subtypes and its variants within 4 h without using anti-serum for immunofix-ation. And new variants Gc1c3 and Gc2c7 are discovered in Chinese people, andtheir frequencies are 0. 0008 and 0. 0004 respectively.

关键词： Gc subtypes Gc variants immunofixation SSA TCA.

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Enhancing chloramphenicol and trimethoprim in vitro activity by Ocimum sanctum Linn.(Lamiaceae) leaf extract against Salmonella enterica serovar Typhi

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Asian Pacific Journal of Tropical Medicine 2012年第3期5卷 220-224页

作者： Shyamapada Mandal Manisha Deb Mandal Nishith Kumar Pal department of Microbiology Bacteriology and Serology UnitCalcutta School of Tropical MedicineC.R.AvenueKolkata-700073India department of Physiology and Biophysics KPC Medical College and HospitalJadavpurKolkata-700032India

Objective:To evaluate the antibacterial activity of Ocimum sanctum(***) leaf extract, alone,and in combination with chloramphenicol(C) and trimethoprim(Tm) against Salmonella enterica serovar Typhi(***).Methods:The antibacterial activity of ethanolic extract of tulsi, ***,leaf(TLE:500μg) for 23 *** isolates was determined following agar diffusion. The C(30μg) and Tm(5μg) activity alone and in combination with TLE(250μg) was determined by disk *** zone diameter of inhibition(ZDI) for the agents was recorded, and growth inhibitory indices(Glls) were ***:The *** isolates(n=23),which were resistant to both C(ZDI 6 mm) and Tm(ZDI 6 mm),had TLE(500μg) ZDIs 16-24 *** ZDIs of C and Tm were increased up to 15-21 mm and 17-23 mm,respectively,when TLE(250μg) was added to the C and Tm *** Glls ranged 0.789-1.235 and 0.894-1.352,due to combined activity against *** isolates,of C and TLE and Tm and ***:The data suggest that TLE,in combination with C and Tm,had synergistic activity for *** isolates, and hence *** is potential in combating *** drug resistance,as well promising in the development of non-antibiotic drug for *** infection.

关键词： Antibacterial activity Ocimum sanctum Chloramphenicol Trimethoprim Growth inhibitory index Synergy Salmonella enterica serovar Typhi

THE STUDY OF INTIMATE DEGREE OF THE FINGERPRINT HEREDITY IN THE CHINESE HAN NATIONALITY

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中国医科大学学报 1988年第S2期 15-22页

作者：林子清宋宏伟贾静涛 Shenyang China Medical University China department of Forensic serology department of Forensic Anthropology

The author studied the hereditary relationship of fingerprint types between parents and childran on the 405 families (total 1612 persons, perents is 810 persons, children is 802 prsons) in Jiaohe county of Jilin province by the method of computer technique and quantitative analysis. According to the frequencies of the fingerprint of parent the fingerprint type is derided into different subtypes, and the relationship between the parents and their children belonging to the certain subtype are analysed in parallel the corresponding subtypes of a common population,With the evaluation of the subtypes of the children, calculated the intimate degree (I.D.)of the children’s fingerprint types, then comparing the results of the supposed families with the one of the realistic families shown evidently the hereditary nature of the fingerprint types. The application cf the I. D. of fingerprint heredity in Forensic paternity testing is a new index in the study of the fingerprint heredity.

关键词： THE STUDY OF INTIMATE DEGREE OF THE FINGERPRINT HEREDITY IN THE CHINESE HAN NATIONALITY than

Serologic evidence of human leptospirosis in and around Kolkata,India:A clinico-epidemiological study

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Asian Pacific Journal of Tropical Medicine 2011年第12期4卷 1001-1006页

作者： Manisha DebMandal Shyamapada Mandal Nishith Kumar Pal department of Bacteriology and serology Calcutta School of Tropical MedicineC.R.AvenueKolkata-700073India department of Physiology and Biophysics KPC Medical College and HospitalKolkata-700032India department of Zoology Gurudas CollegeNarkeldangaKolkata-700054India department of Microbiology Institute of Postgraduate Medical Education and ResearchKolkata-700020India

Objective:To investigate the prevalence of leptospirosis among patients from within and outside ***,attending the Calcutta School of Tropical Medicine,for treatment during August 2002 to August ***:The leptospirosis cases were determined on the basis of clinical, epidemiological,and biochemical factors,and were tested for leptospiral antibodies using IgM *** samples with absorbance ratio≥1.21 were interpreted as ***:The commonest presentation involved fever,headache and *** male-female ratio was 61:46.A total of 65(64.20%) cases had abnormal liver and renal functions respectively,and 57.1% had both the *** highest incidence(75.35.04%) was recorded in SeptemberOctober followed by July-August(53.24.77%).The reactive cases had absorbance ratios between 1.21 and *** 53 showed equivocal result,while IgM non reactivity were seen in 90 patients (absorbance ratios 0.10-0.90).The patients responded to treatment with parenteral antibiotics, penicillin,ceftriaxone and cefotaxime;follow up did not reveal case ***:The cardinal signs of leptospirosis help in making clinical diagnosis,but in any hyper-endemic situation any patient reporting with acute lever and signs of pulmonary,hepatic or renal involvement should be suspected to have leptospirosis and investigated *** awareness,and early diagnosis and treatment,can reduce mortality due to leptospirosis.

关键词： Human leptospirosis IgM ELISA Clinico-epidemiological study Kolkata

THE APPLICATION OF THE THEORY OF QUANTIFICATION(TYPE Ⅰ)IN THE AGE ESTIMATION BY THE TEETH

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中国医科大学学报 1987年第S2期 1-5+14页

作者：宋宏伟贾静涛 China department of Forensic serology department of Forensic Anthropology Shenyang Faculty of Forensic Medicine China Medical University

The data was gathered from 878 cases in Liaoning Province inChina. By applying the theory of quantification type Ⅰand themultiple stepwise regression the mathematic models were estab-lished to estimate age on the basis of teeth attrition degrees, and 4equations for the estimation of age have been obtained by the aidof computer technique as well. These 4 equations,for the conven-ience of usage, have been turned into the tables for estimation ofage. The correlation coefficients are from 0.93 to 0. 96. The test-significance of variance analysis is of great significance(F>F0. 01,P<0. 01). The equations were tested with 25 skulls. The rate ofcoincidence of the results with the real age is as follows: 60～68 % in ±3 years error, 76～88% in ±5 years error, 88～92%in ±7 years error.

关键词： THE APPLICATION OF THE THEORY OF QUANTIFICATION TYPE IN THE AGE ESTIMATION BY THE TEETH Mean

Plasmid mediated antibiotic resistance of Vibrio cholerae Ol biotype E1 Tor serotype Ogawa associated with an outbreak in Kolkata,India

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Asian Pacific Journal of Tropical Medicine 2010年第8期3卷 637-641页

作者： Shyamapada Mandal Manisha DebMandal Nishith Kumar Pal department of Microbiology Bacteriology and Serology UnitCalcutta School of Tropical MedicineC.R.AvenueKolkata-700 073India department of Physiology and Biophysics KPC Medical College and Hospital1F Raja S C Mallick RoadJadavpurKolkata-700 032India

Objective:To determine the antibiotic resistance of Vibrio choleras(***) O1 biotype El Tor serotype Ogawa isolates involved in an outbreak of watery diarrhea in Kolkata,and to explore the role of plasmid in mediating antibiotic ***:Antibiotic susceptibility and minimum inhibitory concentration(MIC) values of antibiotics for the isolated *** 01 Ogawa(n=12) were determined by disk diffusion and agar dilution methods,respectively,using ampicillin(Am),chloramphenicol(C),trimethoprim(Tm),tetracycline(T).erythromycine(Er), nalidixic acid(Nx).ciprofloxacin(Cp),amikacin(Ak) and cefotaxime(Cf).Plasmid curing of multidrug resistant(MDR) *** 01 Ogawa strains was done following ethidium bromide *** electrophoresis,the plasmid DNAs,extracted from the isolated MDR V. cholerae O1 Ogawa strains and their cured derivatives,were visualized and documented in ’gel doc’ ***:The outbreak causing *** O1 Ogawa isolates were MDR as determined by disk diffusion susceptibility test,and MIC *** isolates showed three different drug resistance patterns:AmTmTErNx(for 6 isolates).TmTErCp(for 5 isolates), and AmTniNx(for one isolate),and showed uniform sensitivity to C,Ak and *** loss of plasmids with the concomitant loss of resistance to Am,Tm,T and Er of the isolates occurred following ethidium bromide ***:The current findings suggest that the V. cholerae O1 Ogawa associated with the cholera outbreak were MDR,and resistance to Am,Tm,T and Er among the isolates were plasmid mediated.

关键词： Cholera Outbreak V.cholerae O1 Ogawa Zone diameter of inhibition Minimum inhibitory concentration Multidrug resistant R-plasmid

Synergistic anti - Staphylococcus aureus activity of amoxicillin in combination with Emblica officinalis and Nymphae odorata extracts

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Asian Pacific Journal of Tropical Medicine 2010年第9期3卷 711-714页

作者： Shyamapada Mandal Manisha DebMandal Nishith Kumar Pal Krishnendu Saha department of Microbiology Bacteriology and Serology UnitCalcutta School of Tropical MedicineC.R.AvenueKolkata-700 073India department of Physiology and Biophysics KPC Medical College and Hospital1F Raja S C Mallick RoadJadavpurKolkata-700 032India

Objective:To evaluate the antibacterial activity of Emblica officinalis Gaertn(***: Family:Euphorbiaceae) seed and Nymphae odorala Aiton(***:Family:Nymphaeaceae) stamen extracts,alone and in combination,and in combination with amoxicillin(Ax) against Staphylococcus aureus(***).Methods:Antibacterial activity of ethanolic extracts of amla,***,seed(AMS:500μg) and sapla,***,stamen(SAP:500 p g) for 12 methicillin-resistant ***(MRSA) isolates was determined following agar diffusion:in order to assess the combined antibacterial activity,AMS(250μg) plus SAP(250μg) were *** Ax(10μg) activity alone and in combination with AMS(250μg),and SAP(250μg) was determined by disk *** zone diameters of inhibition(ZDIs) for the agents were recorded,and growth inhibitory indices(GIIs) were ***:The MRSA isolates (n=12) had AMS(500μg) and SAP(500μg) ZDIs of 12-19 mm and 21-24 mm,*** ZDIs(range 24-27 mm) increased by 3-4 mm due to combined action of AMS(250μg) and SAP(250μg) indicating synergy between extracts for MRSA(GII 0.634-0.742).The MRSA isolates were resistant to Ax(ZDI:8-11 mm),which in combination with AMS and SAP had synergistic effect,both due to increased ZDI[mean±SD=(3.5±0.577) mm]and GII(0.631-0.894).Conclusions:The data suggest that the plants,*** and *** alone or in combination,are promising in the development of phytomedicines,which may be used,alone or in combination with the antibiotic. Ax,against MRSA infection.

关键词： Antibacterial activity Plant extract Amoxicillin Emblica officinalis Nimphae odorata Synergy MRSA

Antibacterial activity of honey against clinical isolates of Escherichia coli,Pseudomonas aeruginosa and Salmonella enterica serovar Typhi

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Asian Pacific Journal of Tropical Medicine 2010年第12期3卷 961-964页

Objective:To ascertain the potential antibacterial activity of honey against clinical isolates of Escherichia coli(***),Pseudomonas aeruginosa(***) and Salmonella enterica serovar Typhi(*** serovar Typhi) by in vitro ***:The partial inhibitory concentration(PIC),minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) values of the autoclaved honey(extracted from Apis indica hive by indigenous method) were determined for *** serovar Typhi(n=8:from blood cultute), ***(n=5:from urine culture) and ***(n=5:from pus culture) isolates by in vitro ***:The PICs of the honey tested for the isolates ranged 0.50%-1.25%(v/v) for S. enterica serovar Typhi,0.75%-1.50%(v/v) for *** and 1.00%-1.25%(v/v) for ***, while the MICs ranged 1.75%-3.00%(v/v),3.00%-3.50%(v/v) and 3.50%(v/v),*** *** and *** isolates had MBC value of 4.00%(v/v):the *** serovar Typhi showed MBCs in between 3.00%and 3.50%(v/v).The bactericidal activity of honey was achieved at concentration 3.00%(v/v) for *** serovar Typhi and ***,and at 3.50%(v/v) for P. ***:The excellent antibacterial activity of honey against clinical bacterial isolates indicates the usefulness of honey in clinical practice against bacterial infection.

关键词： Partial inhibitory concentration Minimum inhibitory concentration Minimum bactericidal concentration Honey Clinical bacteria

Septic shock due to cytomegalovirus colitis associated with rituximab use:A case report

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World Journal of Virology 2025年第1期14卷 118-123页

作者： Siddharth Patel Jordan Jay Prutha Pathak Mc Anto Antony Mrudula Thiriveedi department of Internal Medicine Decatur Morgan HospitalDecaturAL 35601United States department of Medicine Alabama College of Osteopathic MedicineDothanAL 36303United States department of Internal Medicine North Alabama Medical CenterFlorenceAL 35630United States department of Endocrinology Diabetes and MetabolismMedical University of South Carolina/AnMed CampusAndersonSC 29621United States

BACKGROUND Cytomegalovirus(CMV)infections can cause significant morbidity and mortality in immunocompromised *** targets dysfunctional *** rituximab(RTX)therapy can cause B-lymphocyte dysfunction,increasing CMV ***,CMV infections present with critical illness such as septic *** SUMMARY A 64-year-old African American woman presented with generalized weakness and non-bloody watery diarrhea of 4-6 weeks *** did not have nausea,vomiting or,abdominal *** had been on monthly RTX infusions for neuromyelitis *** was admitted for septic shock due to *** investigations suggested pancytopenia and serology detected significantly elevated CMV *** treatment led to disease *** This case illustrates an extremely rare case of CMV colitis associated with RTX use presenting with septic *** suspicion for rare opportunistic infections is imperative in individuals with long-term RTX use.

关键词： Cytomegalovirus colitis Rituximab use Immunocompromised status Septic shock Pancytopenia Case report