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Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

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Journal of Innovative Optical Health Sciences 2020年第6期3卷 36-46页

作者： Xiaoyue Liang Zhaohui Zou Zheng Zou Changyi Li Xiaoxi Dong Huijuan Yin Guohui Yan department of Endodontics tianjin medical university stomatology hospital tianjin 300070 P.R.China department of prosthodontics tianjin medical university stomatology hospital tianjin 300070 P.R.China Institute of Biomedical Engineering Chinese Academy of medical Sciences&Peking Union medical College tianjin 300192 P.R.China

The main objective of this study is to evaluate the antibacterial effect of antibacterial pho-todynamic therapy(aPDT)on Streptococcus mutans(***)biofilm model in *** selection of photosensitizers is the key step for the efficacy of photodynamic therapy(PDT).However,no studies have been conducted in the oral field to compare the functional char-acteristics and application effects of PDT mediated by various *** this re-search,the antibacterial effect of Methylene blue(MB)/650 nm laser and Hematoporphyrin monomethyl ether(HMME)/532 nm laser on *** biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two *** yield of lactic acid was analyzed by Colony forming unit(CFU)and spectrophotometry,and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy(CLSM)to evaluate the bactericidal effect on each *** on the results of CFU,the bacterial colonies formed by 30.4J/cm^(2)532nm MB-aPDT group and 30.4J/cm^(2)532nm HMME-aPDT group were significantly less than those in other groups,and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT *** acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control *** activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4 J/cm^(2) ***,aPDT suitable for energy measurement can kill *** plaque biofilm,and MB-aPDT is better than HMME-aPDT.

关键词： Caries Methylene blue(MB) Hematoporyrin monomethyl Ether(HMME) laser antimicrobial photodynamic therapy(aPDT)

Tetrahedral framework nucleic acids promote the proliferation and differentiation potential of diabetic bone marrow mesenchymal stem cell

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Chinese Chemical Letters 2024年第9期35卷 284-288页

作者： Yanjing Li Jiayin Li Yuqi Chang Yunfeng Lin Lei Sui department of prosthodontics Tianjin Medical University School and Hospital of StomatologyTianjin 300070China State Key Laboratory of Oral Diseases West China Hospital of StomatologySichuan UniversityChengdu 610041China

Diabetes mellitus considerably affects bone marrow mesenchymal stem cells(BMSCs),for example,by inhibiting their proliferation and differentiation potential,which enhances the difficulty in endogenous bone ***,effective strategies for enhancing the functions of BMSCs in diabetes have farreaching consequences for bone healing and regeneration in diabetes *** framework nucleic acids(tFNAs)are nucleic acid nanomaterials that can autonomously enter cells and regulate their *** this study,we evaluated the effects of tFNAs on BMSCs from diabetic *** found that tFNAs could promote the proliferation,migration,and osteogenic differentiation of BMSCs from rats with type 2 diabetes mellitus,and inhibited cell senescence and ***,tFNAs effectively scavenged the accumulated reactive oxygen species and activated the suppressed protein kinase B(Akt)signaling ***,we show that tFNAs can recover the proliferation and osteogenic potential of diabetic BMSCs by alleviating oxidative stress and activating Akt *** study provides a strategy for endogenous bone regeneration in diabetes and also paves the way for exploiting DNA-based nanomaterials in regenerative medicine.

关键词： Bone marrow mesenchymal stem cell Diabetes mellitus DNA-based nanomaterial Tetrahedral framework nucleic acids Endogenous bone regeneration

Doping bioactive elements into a collagen scaffold based on synchronous self-assembly/mineralization for bone tissue engineering

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Bioactive Materials 2020年第4期5卷 844-858页

作者： Huanhuan Liu Mingli Lin Xue Liu Ye Zhang Yuyu Luo Yanyun Pang Haitao Chen Dongwang Zhu Xue Zhong Shiqing Ma Yanhong Zhao Qiang Yang Xu Zhang School of stomatology Hospital of StomatologyTianjin Medical UniversityTianjin300070China department of stomatology Zhongshan Hospital of Xiamen UniversityXiamenFujian361004China The Third Central Clinical College of tianjin medical university Tianjin300170China department of Spine Surgery Tianjin HospitalTianjin300211China Institute of stomatology Tianjin Medical UniversityTianjin300070China

Pure collagen is biocompatible but lacks inherent osteoinductive,osteoimmunomodulatory and antibacterial *** obtain collagen with these characteristics,we developed a novel methodology of doping bioactive elements into collagen through the synchronous self-assembly/mineralization(SSM)of *** the SSM model,amorphous mineral nanoparticles(AMN)(amorphous SrCO3,amorphous Ag3PO4,etc.)stabilized by the polyampholyte,carboxymethyl chitosan(CMC),and collagen molecules were the primary components under acidic *** the pH gradually increased,intrafibrillar mineralization occurred via the self-adaptive interaction between the AMNs and the collagen microfibrils,which were self-assembling;the AMNs wrapped around the microfibrils became situated in the gap zones of collagen and finally transformed into *** collagen scaffolds(Sr-CS)promoted in vitro cell proliferation and osteogenic differentiation of rat bone marrow mesenchymal stromal cells(rBMSCs)and synergistically improved osteogenesis of rBMSCs by altering the macrophage ***-doped collagen scaffolds(Ag-CS)exhibited in vitro antibacterial effects on ***,as well as cell/tissue ***,Sr-CS implanted into the calvarial defect of a rat resulted in improved bone ***,the SSM model is a de novo synthetic strategy for doping bioactive elements into collagen,and can be used to fabricate multifunctional collagen scaffolds to meet the clinical challenges of encouraging osteogenesis,boosting the immune response and fighting severe infection in bone defects.

关键词： Collagen scaffold Bioactive elements Synchronous self-assembly/mineralization Bone tissue engineering

Signature Based on Six Autophagy-related Genes to Predict Prognosis of Head and Neck Squamous Cell Carcinoma

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Current medical Science 2022年第3期42卷 597-605页

作者： Hua ZHANG Liang ZHANG Ya-tong FAN Tian-ning LI Li-su PENG Kun-peng WANG Jun MA Clinical Laboratory Diagnostics Tianjin Medical University General Hospital Airport SiteTianjin 300308China department of Health Statistics Public Health School of Tianjin Medical UniversityTianjin 300070China School and hospital of stomatology Tianjin Medical UniversityTianjin 300070China department of Physiology and Pathophysiology Tianjin Medical UniversityTianjin 300070China

Objective The present study aimed to develop an autophagy-related gene prognostic prediction model to provide survival risk prediction for head and neck squamous cell carcinoma(HNSCC)*** The K-mean cluster analysis was performed on HNSCC samples based on the expression values of 210 autophagy-related genes for candidate signature gene *** Cox regression analysis was generated using the potential genes and the risk score was calculated from the prognosis *** risk score was processed as an independent prognostic indicator to construct the nomogram *** immune status including immune cell infiltration ratio and checkpoints of patients with HNSCC in high-and low-risk groups was also *** LASSO Cox regression analysis was performed on the selected autophagy-related *** to the lambda value corresponding to the number of different genes in the LASSO Cox analysis,six genes(GABARAPL2,SAR1A,ST13,GAPDH,FADD and LAMP1)were finally *** risk score based on the genes was generated,which was an independent prognostic marker for *** prognostic prediction model(nomogram)was further optimized by the independent prognostic factors(risk score),which can better predict the prognosis and survival of *** the risk score and prognosis model,eight types of immune cells and six key immune checkpoints(CTLA4,PD1,IDO1,TDO2,LAG3,TIGIT)displayed expression *** This study identified several potential prognostic biomarkers and established an autophagy-related prognostic prediction model for HNSCC,which provides a valuable reference for future clinical research.

关键词： head and neck squamous cell carcinoma autophagy risk score Cox regression nomogram immune cell infiltration ratio

Wet environment-induced adhesion and softening of coenzyme-based polymer elastic patch for treating periodontitis

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Bioactive Materials 2024年第5期35卷 259-273页

作者： Ying Qi Chenyu Xu Zhuodan Zhang Qian Zhang Ziyang Xu Xinrui Zhao Yanhong Zhao Chunyan Cui Wenguang Liu School of Materials Science and Engineering Tianjin Key Laboratory of Composite and Functional MaterialsTianjin UniversityTianjin300350China School and hospital of stomatology Department of OrthodonticsTianjin Medical UniversityTianjin300070China

Periodontitis,a common chronic inflammatory disease caused by pathogenic bacteria,can be treated with diverse biomaterials by loading drugs,cytokines or ***,these biomaterials often show unsatisfactory therapeutic efficiency due to their poor adhesion,short residence time in the wet and dynamic oral cavity and emerging drug *** we report a wet-responsive methacrylated gelatin(GelMA)-stabilized co-enzyme polymer poly(α-lipoic acid)(PolyLA)-based elastic patch with water-induced adhesion and softening *** PolyLA-GelMA,the multiple covalent and hydrogen-bonding crosslinking between PolyLA and GelMA prevent PolyLA depolymerization and slow down the dissociation of PolyLA in water,allowing durable adhesion to oral periodontal tissue and continuous release of LA-based bioactive small molecule in periodontitis wound without resorting external *** with the undifferentiated adhesion behavior of traditional adhesives,this wet-responsive patch demonstrates a favorable periodontal pocket insertion ability due to its non-adhesion and rigidity in dry *** vitro studies reveal that PolyLA-GelMA patch exhibits satisfactory wet tissue adhesion,antibacterial,blood compatibility and ROS scavenging *** the model of rat periodontitis,the PolyLA-GelMA patch inhibits alveolar bone resorption and accelerates the periodontitis healing by regulating the inflammatory *** biomacromolecule-stabilized coenzyme polymer patch provides a new option to promote periodontitis treatment.

关键词： Co-enzyme α-Lipoic acid Wet tissue adhesion Elastic patch Periodontitis

LAMC2 regulates proliferation, migration, and invasion mediated by the Pl3K/AKT/mTOR pathway in oral

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Oncology Research 2023年第4期31卷 481-493页

作者： FAYU SHAN LANLAN LIANG CHONG FENG HONGBAO XU ZIROU WANG WEILI LIU LINGLING PU ZHAOLI CHEN GANG CHEN XINXING WANG department of Environmental Medicine Tianjin Institute of Environmental and Operational MedicineTianjin300070China School and hospital of stomatology Tianjin Medical UniversityTianjin300050China

Background:Oral squamous cell carcinoma(OSCC)is a common malignant ***,Laminin Gamma 2(LAMC2)has been shown to be abnormally expressed in OSCC;however,how LAMC2 signaling contributes to the occurrence and development of OSCC and the role of autophagy in OSCC has not been fully *** study aimed to analyze the role and mechanism of LAMC2 signaling in OSCC and the involvement of autophagy in ***:To explore the mechanism by which LAMC2 is highly expressed in OSCC,we used small interfering RNA(siRNA)to knock down LAMC2 to further observe the changes in the signaling ***,we used cell proliferation assays,Transwell invasion assays,and wound-healing assays to observe the changes in OSCC proliferation,invasion,and ***-LC3 was used to detect the level of autophagy intensity.A cell line-derived xenograft(CDX)model was used to detect the effect of LAMC2 on tumor growth in ***:This study found that the level of autophagy was correlated with the biological behavior of *** downregulation of LAMC2 activated autophagy and inhibited OSCC proliferation,invasion,and metastasis via inhibiting the PI3K/AKT/mTOR ***,autophagy has a dual effect on OSCC,and the synergistic downregulation of LAMC2 and autophagy can inhibit OSCC metastasis,invasion,and proliferation via the PI3K/AKT/mTOR ***:LAMC2 interacts with autophagy to regulate OSCC metastasis,invasion,and proliferation via the PI3K/AKT/mTOR ***2 down-regulation can synergistically modulate autophagy to inhibit OSCC migration,invasion,and proliferation.

关键词： LAMC2 OSCC Autophagy PI3K/AKT/mTOR pathway 3-Methyladenine Rapamycin

Continuously Quantifying Oral Chemicals Based on Flexible Hybrid Electronics for Clinical Diagnosis and Pathogenetic Study

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Research 2022年第4期2022卷 71-86页

作者： Wei Lingo Yinghui Wang ingyu Lu Xue Shang Ziyue Wu Zhaorun Chen Xueting Li Chenchen Zou Jinjie Yan Yunjie Zhou Jie Liu Hongjie Li Kehua Que Xian Huan department of Biomedical Engineering Tianjin University92 Weijin RoadTianjin 300072China Center of Flexible Wearable Technology Institute of Flexible Electronic Technology of TsinghuaJiaxing 314006China School of stomatology Hospital of StomatologyTianjin Medical University12 Observatory RoadTianjin 300070China

Simultaneous monitoring of diverse salivary parameters can reveal underlying mechanisms of intraoral biological processes and offer profound insights into the evolution of oral ***,conventional analytical devices with bulky volumes,rigid formats,and discrete sensing mechanisms deviate from the requirements of continuous biophysiological quantification,resulting in huge difficulty in precise clinical diagnosis and pathogenetic ***,we present a flexible hybrid electronic system integrated with functional nanomaterials to continuously sense Ca^(2+),pH,and temperature for wireless real-time oral health *** miniaturized system with an island-bridge structure that is designed specifically to fit the teeth is only 0.4g in weight and 31.5×8.5×1.35 mm^(3) in dimension,allowing effective integration with customized dental braces and comfort attachment on *** results indicate high sensitivities of 30.3 and 60.6 mV/decade for Ca^(2+)and pH with low potential *** system has been applied in clinical studies to conduct Ca^(2+)and pH mappings on carious teeth,biophysiological monitoring for up to 12 h,and outcome evaluation of dental restoration,providing quantitative data to assist in the diagnosis and understanding of oral ***,caries risk assessment of 1o human subjects using the flexible system validates the important role of saliva buffering capacity in caries *** proposed flexible system may offer an open platform to carry diverse components to support both clinical diagnosis and treatment as well as fundamental researchfororaldiseases and induced systemicdiseases.

关键词： diagnosis precise teeth

Deregulation of tumor suppressive ASXL1−PTEN/AKT axis in myeloid malignancies

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Journal of Molecular Cell Biology 2020年第9期12卷 688-699页

作者： Lei Cao Xianyou Xia Yu Kong Fengqin Jia Bo Yuan Rui Li Qian Li YuxinWang Mingrui Cui Zhongye Dai Huimin Zheng Jesper Christensen Yuan Zhou Xudong Wu Collaborative Innovation Center of tianjin for medical Epigenetics Tianjin Key Laboratory of Medical EpigeneticsKey Laboratory of Immune Microenvironment and Disease(Ministry of Education)Department of Cell BiologyTianjin Medical UniversityTianjin 300070China National Demonstration Center for Experimental Basic medical Science Education Tianjin Medical UniversityTianjin 300070China department of prosthodontics School and Hospital of StomatologyTianjin Medical UniversityTianjin 300070China Biotech Research and Innovation Centre and Centre for Epigenetics University of Copenhagen2200 CopenhagenDenmark State Key Laboratory of Experimental Hematology Institute of Hematology and Blood Diseases HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300020China

Mutations of epigenetic regulators are pervasive in human ***1 is frequently mutated in myeloid *** previously found that ASXL1 forms together with BAP1 a complex that can deubiquitinylate mono-ubiquitinylated lysine 119 on histone H2A(H2AK119ub1),a Polycomb repressive ***,a complete mechanistic understanding of ASXL1 in transcriptional regulation and tumor suppression remains to be ***,we find that depletion of Asxl1 confers murine 32D cells to IL3-independent growth at least partly due to sustained activation of PI3K/AKT ***,Asxl1 is critical for the transcriptional activation of Pten,a key negative regulator of AKT *** we confirm that Asxl1 is specifically enriched and required for H2AK119 deubiquitylation at the Pten ***,ASXL1 and PTEN expression levels are positively correlated in human blood cells and ASXL1 mutations are associated with lower expression levels of PTEN in human myeloid ***,malignant cells with ASXL1 downregulation or mutations exhibit higher sensitivity to the AKT inhibitor ***,this study has linked the PTEN/AKT signaling axis to deregulated epigenetic changes in myeloid *** also provides a rationale for mechanism-based therapy for patients with ASXL1 mutations.

关键词： tumor suppressor Polycomb H2A ubiquitylation PTEN AKT

Prospects and challenges of dynamic DNA nanostructures in biomedical applications

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Bone Research 2022年第3期10卷 454-468页

作者： Taoran Tian Yanjing Li Yunfeng Lin State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041P.R.China department of prosthodontics Tianjin Medical University School and Hospital of StomatologyTianjin 300070P.R.China

The physicochemical nature of DNA allows the assembly of highly predictable structures via several fabrication strategies, which have been applied to make breakthroughs in various fields. Moreover, DNA nanostructures are regarded as materials with excellent editability and biocompatibility for biomedical applications. The ongoing maintenance and release of new DNA structure design tools ease the work and make large and arbitrary DNA structures feasible for different applications. However, the nature of DNA nanostructures endows them with several stimulus-responsive mechanisms capable of responding to biomolecules, such as nucleic acids and proteins, as well as biophysical environmental parameters, such as temperature and p H. Via these mechanisms, stimulus-responsive dynamic DNA nanostructures have been applied in several biomedical settings, including basic research, active drug delivery, biosensor development, and tissue engineering. These applications have shown the versatility of dynamic DNA nanostructures, with unignorable merits that exceed those of their traditional counterparts, such as polymers and metal ***, there are stability, yield, exogenous DNA, and ethical considerations regarding their clinical translation. In this review, we first introduce the recent efforts and discoveries in DNA nanotechnology, highlighting the uses of dynamic DNA nanostructures in biomedical applications. Then, several dynamic DNA nanostructures are presented, and their typical biomedical applications,including their use as DNA aptamers, ion concentration/p H-sensitive DNA molecules, DNA nanostructures capable of strand displacement reactions, and protein-based dynamic DNA nanostructures, are discussed. Finally, the challenges regarding the biomedical applications of dynamic DNA nanostructures are discussed.

关键词： particles dynamic breakthrough