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Persistence and Impact of a PGPR on Microbial Communities of Biosolids and soil Amended with Them

Journal of Environmental science and Engineering 2011年第5期5卷 578-595页

作者： L. Fracchia E.B.R. Perotti A. Pidello M. Rinaldi M.G. Martinotti department of Chemical Food Pharmaceutical and Pharmacological Sciences Drug and Food Biotechnology Centre Universityof Eastern Piedmont Novara 28100 Italy Laboratory of Biological Chemistry L FCV-Consejo de lnvestigaciones National University of Rosario Casilda Santa Fe 2170Argentina

Survival of the plant-growth promoting rhizobacteria （PGPR） strain Pseudomonasfluorescens 92 in two batches ofbiosolids and soil amended with them and functional and structural shifts occurring in the resident bacterial communities were assessed. Viability of the rifampicin resistant gfp-tagged P. fluorescens 92RTcgfp, inoculated in microcosms consisting of biosolids, soil amended with biosolids and soil, drastically decreased 3-7 days after inoculation in treatments containing biosolids and soil amended with them. The PGPR counts in biosolids were always lower than in soil throughout the experiment whereas, in soil amended with biosolids, were similar or higher depending on the batch of biosolid. Analysis of the community-level physiological profiling （CLPP） revealed a strong impact of the strain on the metabolic activities, as seen in a general uniformity of the inoculated microcosms profiles. According to single-strand conformation polymorphism （SSCP） analysis, the presence of the PGPR had no effect on the resident soil and biosolid eubacterial population, whereas it induced a transient shift in the composition of the resident pseudomonads. In conclusion, biosolids themselves are not suitable as inoculum vehicle for the PGPR nevertheless, their combined incorporation into soil increases the inoculum survival and buffers the competition effects with the resident communities.

关键词： plant-growth promoting rhizobacteria bacterial communities inoculum survival biolog ecoplatestm single-strandconformation polymorphism.

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聚丙烯酸盐对长期重金属污染的矿区土壤的修复研究(Ⅱ)--对土壤微生物数量和土壤酶活性的影响

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农业环境科学学报 2009年第4期28卷 653-657页

作者：曲贵伟 Amarilis de Varennes 依艳丽沈阳农业大学土地与环境学院辽宁沈阳110161 department of Agricultural and Environmental Chemistry Institute Superior De AgronomiaTechnical University of LisbonLisbon 1349-107Protugal

采用盆栽试验方法研究了聚丙烯酸盐对S Domingos金矿区重金属污染土壤的修复作用。在《聚丙烯酸盐对长期重金属污染土壤的修复研究(Ⅰ)》[30](以下简称研究Ⅰ)中聚丙烯酸盐的应用显著提高了土壤持水能力和土壤的pH,同时土壤中水溶性重... 详细信息

采用盆栽试验方法研究了聚丙烯酸盐对S Domingos金矿区重金属污染土壤的修复作用。在《聚丙烯酸盐对长期重金属污染土壤的修复研究(Ⅰ)》[30](以下简称研究Ⅰ)中聚丙烯酸盐的应用显著提高了土壤持水能力和土壤的pH,同时土壤中水溶性重金属的含量也比对照显著降低,从而使果园草Orchardgrass(Dactylis glomerata ***)的生物量得到显著增加。为更全面地说明聚丙烯酸盐在该重金属污染土壤修复上的作用,进一步探讨了聚丙烯酸盐对重金属污染土壤微生物属性的影响。结果表明,土壤细菌数量随聚丙烯酸盐水平的增加而增加,不同处理之间差异显著(P≤0.05);与对照相比,聚丙烯酸盐处理的真菌数量显著增加(P≤0.05),但随着聚丙烯酸盐水平的增加而显著降低(P≤0.05),这可能是由于0.4%和0.6%的聚丙烯酸盐处理土壤pH显著提高所导致的。因此使用微生物数量评价土壤质量时,细菌比真菌表现更突出。使用聚丙烯酸盐修复的土壤脱氢酶、磷酸酶、蔗糖酶、蛋白酶和纤维素酶的活性均比对照显著增加(P≤0.05),而脲酶活性的下降则可能是由于聚丙烯酸盐带来大量的铵态氮产生的抑制作用所致。综合研究Ⅰ中的结果,聚丙烯酸盐不仅可以改善重金属污染土壤的理化性状而且对于土壤微生物属性也有显著的改善作用。从果园草生物量来看,0.4%聚丙烯酸盐的用量对该污染土壤的修复具有实践应用价值。

关键词：聚丙烯酸盐矿区土壤修复重金属土壤微生物数量土壤酶活性

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Effect of Food Waste Compost (FWC) and its Non-Aerated Fermented Extract (NFCE) on Seeds Germination and plant Growth

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Open Journal of soil science 2021年第2期11卷 122-138页

作者： Raja Jarboui Bilel Dhouib Emna Ammar Faculty of sciences Department of Biology Jouf University Sakaka Saudi Arabia Laboratory of Environment sciences and Sustainable Development University of Sfax Preparatory Institute of Engineering Studies of Sfax Sfax Tunisia Faculty of sciences of Sfax Sfax Tunisia National Engineering School of Sfax Sfax Tunisia

The aim of this study was to investigate the effect of the food wastes compost (FWC) and its non-aerated fermented extract (NFCE) on seed germination and growth of tomato (Solanum lycopersicum L.), watercress (Nasturtium officinale), chili pepper (Capsicum annuum), peas (Pisum sativum L.), chickpea (Cicer arietinum) and beans (Vicia faba) under greenhouse conditions. The FWC and NFCE were physico-chemically and microbiologically characterized. The NFCE effect was evaluated on tomato, watercress, and chili pepper seeds germination and seedling growth. However, for leguminous, pea, chickpea and bean seedlings, the FWC amended soils and irrigated with NFCE were tested for plants growth. The results of FWC analyses revealed that FWC has neutral pH, low EC and C/N ratio, with fertilizing elements (N, P, K and Mg) and lack of phytotoxic effect. The NFCE was characterized by low EC and relatively high carbon content (COD = 9700 mg/l), and intense microbial activity, notably mesophilic bacteria. Therefore, in fermented compost extract, mesophilic bacteria were increased by 225, yeasts by 25 and molds by 10 times compared to those of the investigated compost. In greenhouse, the diluted NFCE increased significantly (psoil or the NFCE irrigated soil, improved the growth of tested seedlings. The use of soil amended with compost and irrigated by fermented compost extract decreased significantly the growth of the same experimented seedlings. Therefore, the FWC and its fermented extract were a suitable substrate for germination and growth of the studied seeds.

关键词： Food Wastes Compost Fermented Compost Extract Seed Germination Seedling Growth

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Heavy metal pollution in farmland irrigated with river water near a steel plant——magnetic and geochemical signature

Heavy metal pollution in farmland irrigated with river water...

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中国科学院地质与地球物理研究所2013年度（第13届）学术年会

作者： Chunxia Zhang Erwin Appel Qingqing Qiao State Key Laboratory of Litho spheric Evolution Institute of Geology and GeophysicsChinese Academy of Sciences Key Laboratory of Cenozoic Geology and Environment Institute of Geology and GeophysicsChinese Academy of Sciences department of Geosciences University of Tuebingen

The presence of heavy metals(HMs) in the environment is a major threat for *** proxies provide a rapid method for assessing the degree of HM pollution in environment. We have studied farmland soil irrigated with polluted river water in the vicinity of a steel plant in Loudi city(Hunan Province,China) to test the efficiency of magnetic methods for detecting the degree of HM *** magnetic and non-magnetic(microscopic,chemical and statistical) methods were used to characterize these farmland *** magnetic concentration values were found in the upper arable soil horizon(0-20 cm),which is related to the presence of spherical～10 to 30μm sized magnetite *** spatial distribution of magnetic concentration and HM contents in the farmland soils matches with the spatial pattern of these parameters in river *** findings provide evidence that HM pollution of the farmland soil is mainly caused by irrigation with *** Zn,Pb,Cu,Cd,Co, Ni,V are well correlate with magnetic susceptibility(χ).The pollution load index(PLI) of all nine anthropogenic HMs(including also Cr and Mo) and log(χ) are significantly correlated. Using the resulting linear PLI-log(χ) function,values ofχcan serve as a convenient tool for semi-quantifying the degree of HM pollution in the uppermost～20 cm of the studied farmland *** findings suggest that magnetic methods can generally serve as a convenient tool for detecting and mapping HM pollution in farmland soil irrigated with wastewater from sites nearby heavy industrial activities.

关键词： Environmental magnetism Rock and mineral magnetism Asia

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Microbial Diversity in Rhizosphere soil of Cotinus coggygria Based on High Throughput Sequencing

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plant Diseases and Pests 2019年第5期10卷 11-15,23页

作者： Zhou Jianghong Xia Fei Che Shaochen Ge Yuxuan Zhou Xiaohong Beijing Key Laboratory of Ecological Function Assessment and Regulation Technology of Green Space Beijing Institute of Landscape ArchitectureBeijing 100102China Management department of Beijing Fragrant Hills Park Beijing 100093China Beijing Florascape Co. Ltd.Beijing 100044China

In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively,were conducted by high throughput sequencing(Illumina MiSeq).The results showed that the fungal diversity in the rhizosphere soil samples of *** in Fragrant Hills park in 2018 was significantly higher than that in 2016,and it was higher in the rhizosphere soil of healthy *** in Xunlupo than that in diseased ones in *** dahliae,which is the causal agent of *** wilt,was detected in five soil *** 2018,the bacterial diversity in the rhizosphere soil of diseased *** in Xunlupo was the lowest,while it was the highest in the rhizosphere soil of healthy *** under Platycladus orientalis in Langfengting.

关键词： Cotinus coggygria Verticillium wilt Rhizosphere soil Microbial diversity High throughput sequencing

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soil amendment strategies determining microbial community composition and their assembly processes in a continuously cropped soil

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soil Ecology Letters 2024年第3期6卷 85-95页

作者： Hongkai Liao Chunli Zheng Juan Li Jian Long Yaying Li Guizhou Provincial Key Laboratory of Mountain Environment Guizhou Normal UniversityGuiyang 550001China The Key Laboratory of Environmental Pollution Monitoring and Disease Control Ministry of EducationDepartment of ToxicologySchool of Public HealthGuizhou Medical UniversityGuiyang 550025China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China Key Laboratory of Urban Environmental Processes and Pollution Control Ningbo(Beilun)Zhongke Haixi Industry Technology Innovation CenterNingbo 315800China

Reductive soil disinfestation(RSD)is an important tool for sustainable agricultural ***,the differences in soil bacterial communities and their community assembly processes among RSD and other treatment strategies(e.g.,biochar and chemical fumigation)are still subject to open *** this study,soils subjected to various treatments–un-treated control(CK),chemical soil fumigation with CaCN_(2)(CF),1% biochar(1%B),3% biochar(3%B),and reductive soil disinfestation(RSD)are *** samples were collected,incubated,and then used for growth of tomato *** Sloan neutral community model indicates that stochastic processes dominate in bacterial community assembly for both biochar and CF *** contrast,this work shows that RSD treatment can have a strong impact on soil bacterial community *** relative abundance of Firmicutes increased during unplanted soil incubation,whereas Proteobacteria and Bacteroidetes dominated in the rhizosphere after planting of *** stochasticity ratio reveals that deterministic selection played an important role in the bacterial assembly under RSD *** found that RSD amendment yielded lower biomass than that for other treatments after 28 days of tomato *** results suggest that although RSD treatment has great potential to rebuild soil bacterial ecology by shaping bacterial communities and their assembly processes,it is important to monitor and manage soil conditions(e.g.,soil nutrients or physical properties)before planting to ensure plant productivity.

关键词： amendment strategy reductive soil disinfestation bacterial community assembly processes

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GWLS-SVR模型的红枣树叶片叶绿素含量估算

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光谱学与光谱分析 2021年第6期41卷 1730-1736页

作者：尼格拉·吐尔逊苏磊·乃比高健沈江龙郑江华余丹林新疆大学资源与环境科学学院新疆乌鲁木齐830042 新疆大学数学与系统科学学院新疆乌鲁木齐830046 新疆林业科学院现代林业研究所新疆乌鲁木齐830063 department of Earth and Environmental Studies Montclair State UniversityNew Jersey 07043USA

叶绿素含量是红枣树光合作用能力、生长状况、营养状况的指示剂,不同地理位置种植的红枣树受到自然、人为等因素的影响,叶绿素含量分布有所不同,该研究实地测定了若羌县枣树叶片高光谱反射率及表征叶绿素含量的枣树叶片SPAD(soil plant ... 详细信息

叶绿素含量是红枣树光合作用能力、生长状况、营养状况的指示剂,不同地理位置种植的红枣树受到自然、人为等因素的影响,叶绿素含量分布有所不同,该研究实地测定了若羌县枣树叶片高光谱反射率及表征叶绿素含量的枣树叶片SPAD(soil plant analysis development)值。为了高效无损地估算红枣树叶片SPAD值,计算了红枣树叶片SPAD值全局莫兰指数,以SPAD值和高光谱波段之间的相关性为基础,通过C P统计量计算重要程度高的特征波段,运用地理加权最小二乘支持向量回归GWLS-SVR(geographically weighted least squares-support vector regression)模型对红枣树叶片SPAD值进行预测,与多元线性回归(MLR)、支持向量机回归(SVR)模型比较并探讨GWLS-SVR模型估算红枣树叶片SPAD值的能力。结果表明:(1)光谱一阶导数可以有效去除噪声并突出光谱信息尤其是492~510,542~543,642~652,657~670和682~692 nm区间内显著的提高了与SPAD值的相关性。(2)C P统计量方法能够有效的选择敏感区间的特征波段,进而提高模型估算精度,由统计量方法计算出原始光谱重要程度最高的两个变量为595与696 nm,光谱一阶导数的特征波段为688 nm。其中对于同一个敏感波段区间的波段组合总有单个波段的统计量低于多个波段组合的统计量,这可能是相近波段间的较强共线性导致的。(3)若羌县红枣树叶片SPAD值存在显著的空间聚集性,全局莫兰指数为0.1258(p<0.1),适合建立考虑空间位置的GWLS-SVR模型。(4)结合Bootstrap再抽样与t检验模型检验得到结合地理位置信息的GWLS-SVR模型总体上估算能力优于SVR和MLR模型,且结果高度显著(p<0.001),其中基于光谱一阶导数的GWLS-SVR模型为最优的红枣树叶片SPAD值估算模型(R 2为0.975,MSE为1.082),能够为高光谱定量反演红枣树SPAD值进而快速无损的监测红枣生长状况提供一定参考。

关键词：叶绿素含量 GWLS-SVR模型高光谱红枣树 Bootstrap再抽样 t检验 SPAD值

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土壤数据对分布式流域水文模型模拟效果的影响

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水科学进展 2011年第2期22卷 168-174页

作者：李润奎朱阿兴秦承志陈腊娇刘军志中国科学院地理科学与资源研究所资源与环境信息系统国家重点实验室北京100101 中国科学院研究生院资源与环境学院北京100049 department of Geography University of Wisconsin-Madison

基于空间半分布式的SWAT模型和全分布式的WetSpa Extension模型,分析了高分辨率的SoLIM数字土壤数据和美国SSURGO传统土壤数据对流域径流空间分布式模拟和总量模拟的影响。结果显示:利用不同土壤数据得到的产水模拟结果在空间上差别明显... 详细信息

基于空间半分布式的SWAT模型和全分布式的WetSpa Extension模型,分析了高分辨率的SoLIM数字土壤数据和美国SSURGO传统土壤数据对流域径流空间分布式模拟和总量模拟的影响。结果显示:利用不同土壤数据得到的产水模拟结果在空间上差别明显,但模拟径流总量的差别随着模拟面积的增大而逐渐减小。根据实验结果,对于注重空间分布的模拟目的,需要较详细输入数据的支持;对于总量模拟,则可随模拟面积的增大而适当放宽对输入数据分辨率的要求。

关键词：土壤数据分布式水文模型径流模拟综合作用尺度

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保护性耕作对旱作农田耕层土壤肥力及酶活性的影响

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植物营养与肥料学报 2009年第5期15卷 1085-1092页

作者：罗珠珠黄高宝 Li Guangdi 张仁陟蔡立群甘肃农业大学资源与环境学院甘肃兰州730070 甘肃农业大学农学院甘肃兰州730070 NSW department of Primary Industries Wagga Wagga NSW 2650 Australia

通过田间定位试验,研究了不同耕作方式对黄土高原西部旱农区耕层土壤肥力和酶活性的影响。结果表明,秸秆还田可以显著提高0—5和5—10 cm土层有机质、全氮、全磷、全钾、铵态氮、速效磷、速效钾和3种水解酶活性;10—30 cm土层仅提高了... 详细信息

通过田间定位试验,研究了不同耕作方式对黄土高原西部旱农区耕层土壤肥力和酶活性的影响。结果表明,秸秆还田可以显著提高0—5和5—10 cm土层有机质、全氮、全磷、全钾、铵态氮、速效磷、速效钾和3种水解酶活性;10—30 cm土层仅提高了有机质、全钾和速效钾含量,对其余各养分含量和水解酶活性并无明显影响。免耕降低了0—5、5—10和10—30 cm土层硝态氮含量,但对过氧化氢酶活性有明显促进作用。相关分析表明,土壤有机质、养分和碱性磷酸酶、蔗糖酶活性之间呈极显著相关关系。进一步应用主成分分析表明,土壤有机质、养分和水解酶活性共同反映着黄土高原雨养农区土壤肥力水平的高低。

关键词：免耕秸秆还田旱作农田土壤养分土壤酶活性

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Drought stress and plant ecotype drive microbiome recruitment in switchgrass rhizosheath

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Journal of Integrative plant Biology 2021年第10期63卷 1753-1774页

作者： Tie-Yuan Liu Nenghui Ye Xinyu Wang Debatosh Das Yuxiang Tan Xiangkai You Mingxiu Long Tianming Hu Lei Dai Jianhua Zhang Mo-Xian Chen College of Grassland Agriculture Northwest A&F UniversityYangling 712100China Co-Innovation Center for Sustainable Forestry in Southern China College of Biology and the EnvironmentNanjing Forestry UniversityNanjing 210037China CAS Key Laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China College of AgricultureHunan Agricultural UniversityChangsha 410128China State Key Laboratory of Agrobiotechnology The Chinese University of Hong KongShatinHong Kong 999077China department of Biology Hong Kong Baptist UniversityKowloonHong Kong 999077China

The rhizosheath,a layer of soil grains that adheres firmly to roots,is beneficial for plant growth and adaptation to drought *** is a perennial C4 grass which can form contact rhizosheath under drought *** this study,we characterized the microbiomes of four different rhizocompartments of two switchgrass ecotypes(Alamo and Kanlow)grown under drought or well-watered conditions via 16S ribosomal RNA amplicon *** four rhizocompartments,the bulk soil,rhizosheath soil,rhizoplane,and root endosphere,harbored both distinct and overlapping microbial *** root compartments(rhizoplane and root endosphere)displayed low-complexity communities dominated by Proteobacteria and *** to bulk soil,Cyanobacteria and Bacteroidetes were selectively enriched,while Proteobacteria and Firmicutes were selectively depleted,in rhizosheath *** from Proteobacteria or Firmicutes were specifically selected in Alamo or Kanlow rhizosheath *** drought stress,Citrobacter and Acinetobacter were further enriched in rhizosheath soil,suggesting that rhizosheath microbiome assembly is driven by drought ***,the ecotype-specific recruitment of rhizosheath microbiome reveals their differences in drought stress ***,these results shed light on rhizosheath microbiome recruitment in switchgrass and lay the foundation for the improvement of drought tolerance in switchgrass by regulating the rhizosheath microbiome.

关键词： 16S rRNA sequencing rhizosphere rhizosheath formation switchgrass(Panicum virgatum L.) water deficiency

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