检索结果-南通市图书馆

Journal of Integrative plant Biology 2009年第1期51卷 35-44页

作者： Yingyin Yao Zhongfu Ni Jinkun Du Zongfu Han Yan Chen Qingbo Zhang Qixin Sun department of plant genetics & breeding and state key laboratory for agrobiotechnology Key Laboratory of Crop Heterosis and UtilizationMinistry of Education Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agdculture Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

Differential gene expression between hybrids and their parents is considered to be associated with heterosis. However, the physiological functions and possible contribution to heterosis of these differentially expressed genes are unknown. We have isolated one hybrid upregulated gene encoding putative wheat ADP-ribosylation factor, designated TaARF. In this study, real-time quantitative reverse transcription-polymerase chain reaction analysis indicated that the TaARF transcript was preferentially expressed in root, node and crown, and the accumulation of TaARF mRNA in hybrid was more than 1.5-fold higher than that in two parents. In order to understand possible roles of the putative wheat ARF gene, TaARF was overexpressed in Arabidopsis, and the transgenic plants were characterized. We show that ectopic overexpression of TaARF in Arabidopsis leads to increased leaf area, increased growth rate and earlier transition to flowering, suggesting that TaARF plays significant roles in growth and development. This study provides evidence demonstrating that TaARF plays important roles in growth and development and we speculate that the upregulated expression of this gene might contribute to the heterosis observed in wheat root and leaf growth.

关键词： adenosine diphosphate-ribosylation factor Arabidopsis ectopic heterosis overexpression wheat.

Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem L.)

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2008年第6期18卷 697-705页

作者： Hualing Wu,Zhongfu Ni,Yingyin Yao,Ganggang Guo,Qixin Sun* department of plant genetics&breeding and state key laboratory for agrobiotechnology,key laboratory of Crop Heterosis and Utilization,Ministry of Education,key laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing key laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100094,China department of plant genetics & breeding and state key laboratory for agrobiotechnology Key Laboratory of Crop Heterosis and Utilization Ministry of Education Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

WRKY proteins are involved in various physiological processes,including biotic and abiotic stress responses,hormone responses and ***,no systematic identification,expression and function analysis of WRKY genes in wheat were *** this study,we isolated 15 wheat cDNAs with complete open reading frame(ORF)encoding putative WRKY proteins using in silico *** analysis indicated that the 15 wheat WRKY genes belonged to three major WRKY *** analysis revealed that most genes expressed drastically in leaf,except TaWRKY10 which expressed in crown *** genes were strongly up-reg-ulated with the senescence of *** genes were responsive to low temperature,high temperature,NaCl or PEG ***-over,dierential expression patterns were also observed between wheat hybrid and its parents,and some genes were more responsive to PEG treatment in the *** results demonstrated that wheat WRKY genes are involved in leaf senescing and abiotic *** the changed expression of these WRKY genes in hybrid might contribute to the heterosis by improving the stress tolerance in hybrids.

关键词： Wheat WRKY transcription factor Gene cloning Gene expression Heterosis

plant immune signaling: Advancing on two frontiers

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2020年第1期62卷 2-24页

作者： Wei Wang Baomin Feng Jian-Min Zhou Dingzhong Tang state key laboratory of Ecological Control of Fujian-Taiwan Crop Pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou 350002China The state key laboratory of plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China

plants have evolved multiple defense strategies to cope with pathogens, among which plant immune signaling that relies on cell-surface localized and intracellular receptors takes fundamental roles. Exciting breakthroughs were made recently on the signaling mechanisms of pattern recognition receptors(PRRs) and intracellular nucleotide-binding site(NBS) and leucine-rich repeat(LRR)domain receptors(NLRs). This review summarizes the current view of PRRs activation, emphasizing the most recent discoveries about PRRs’ dynamic regulation and signaling mechanisms directly leading to downstream molecular events including mitogen-activated protein kinase(MAPK) activation and calcium(Ca2+) burst. plants also have evolved intracellular NLRs to perceive the presence of specific pathogen effectors and trigger more robust immune responses. We also discuss the current understanding of the mechanisms of NLR activation, which has been greatly advanced by recent breakthroughs including structures of the first full-length plant NLR complex, findings of NLR sensor-helper pairs and novel biochemical activity of Toll/interleukin-1 receptor(TIR) domain.

关键词： breakthrough activation frontier

Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon

在线全文

学校读者我要写书评

暂无评论

Journal of genetics and Genomics 2011年第12期38卷 593-601页

作者： Lei Tian,Lubin Tan,Fengxia Liu,Hongwei Cai,Chuanqing Sun state key laboratory of plant Physiology and Biochemistry,National Centre for Evaluation of Agricultural Wild plant(Rice), laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing key laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,department of plant genetics and breeding,China Agricultural University,Beijing 100193,China state key laboratory of plant Physiology and Biochemistry National Centre for Evaluation of Agricultural Wild Plant (Rice) Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing key laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture Department of Plant Genetics and Breeding China Agricultural University Beijing 100193 China

Soil salinity is one of the major abiotic stresses affecting plant growth and crop *** the present study,salt tolerance at rice seedling stage was evaluated using 87 introgression lines（ILs）,which were derived from a cross between an elite indica cultivar Teqing and an accession of common wild rice（Oryza rufipogon Griff.）.Substantial variation was observed for four traits including salt tolerance score（STS）, relative root dry weight（RRW）,relative shoot dry weight（RSW） and relative total dry weight（RTW）.STS was significantly positively correlated with all other three traits.A total of 15 putative quantitative trait loci（QTLs） associated with these four traits were detected using single-point analysis,which were located on chromosomes 1,2,3,6,7,9 and 10 with 8%-26%explaining the phenotypic *** O. rufipogon-derived alleles at 13 QTLs（86.7%） could improve the salt tolerance in the Teqing *** QTL clusters affecting RRW, RSW and RTW were found on chromosomes 6,7,9 and 10,*** these four QTL clusters,a major cluster including three QTLs （qRRWIO,qRSWIO and qRTWIO） was found near the maker RM271 on the long arm of chromosome 10,and the ***-derived alleles at these three loci increased RRW,RSW and RTW with additive effects of 22.7%,17.3%and 18.5%,respectively,while the phenotypic variance explained by these three individual QTLs for the three traits varied from 19%to 26%.In addition,several salt tolerant ILs were selected and could be used for identifying and utilizing favorable salt tolerant genes from common wild rice and used in the salt tolerant rice breeding program.

关键词： Common wild rice(O.rufipogon Griff.) Introgression lines Salt tolerance Seedling stage Quantitative trait locus

Analysis of genes related to xylem cell wall development based on transcriptomics in Populus alba ‘Berolinensis’ tension wood

在线全文

学校读者我要写书评

暂无评论

Journal of Forestry Research 2024年第6期35卷 328-343页

作者： Lin Feng Youchao He Xu Li Meiqi Zhou Chao Wang state key laboratory of Tree genetics and breeding Northeast Forestry University150040HarbinPeople’s Republic of China

Populus alba‘Berolinensis’is a fast-growing,high-yielding species with strong biotic and abiotic stress resistance,and widely planted for timber,shelter belts and aesthetic *** this study,molecular development is explored and the important genes regulating xylem forma-tion in ***‘Berolinensis’under artificial bending treat-ments was *** investigation indicated that tension wood(TW)was characterized by eccentric growth of xylem and was enriched in cellulose;the degree of ligni-fication was lower than for normal wood(NW)and oppo-site wood(OW).RNA-Seq-based transcriptome analysis was performed using developing xylem from three wood types(TW,OW and NW).A large number of differentially expressed genes(DEGs)were screened and 4889 *** GO and KEGG enrichment results,genes involved in plant hormone signal transduction,phenylpropanoid biosynthesis,and cell wall and secondary cell wall biogenesis play major roles in xylem development under artificial *** expansin(PalEXP)genes were identified from the RNA-seq data;four were differentially expressed during tension wood *** analysis indicated that PalEXLB1 belongs to the EXPB subfamily and that the other PalEXPs are members of the EXPA subfamily.A transcriptional regulatory network construction showed 10 transcription factors located in the first and second layers upstream of EXP,including WRKY,ERF and ***‒qPCR analy-sis in leaves,stems and roots combined with transcriptome analysis suggests that PalEXPA2,PalEXPA4 and PalEXPA15 play significant regulatory roles in cell wall formation during tension wood *** candidate genes involved in xylem cell wall development during tension wood formation marks an important step toward identifying the molecular regulatory mechanism of xylem development and wood property improvement in ***‘Berolinensis’.

关键词： Populus alba‘Berolinensis’ Tension wood Differentially expressed genes Expansin proteins

The LRXs-RALFs-FER module controls plant growth and salt stress responses by modulating multiple plant hormones

在线全文

学校读者我要写书评

暂无评论

National Science Review 2021年第1期8卷 40-55页

作者： Chunzhao Zhao Wei Jiang Omar Zayed Xin Liu Kai Tang Wenfeng Nie Yali Li Shaojun Xie Yuan Li Tiandan Long Linlin Liu Yingfang Zhu Yang Zhao Jian-Kang Zhu Shanghai Center for plant Stress Biology and Center of Excellence in Molecular plant Sciences Chinese Academy of Sciences department of Horticulture and Landscape Architecture Purdue University key laboratory of plant Stress Biology School of Life SciencesHenan University National Center for Soybean Improvement Key Laboratory of Biology and Genetics and Breeding for Soybean Ministry of Agriculture State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University genetics department Faculty of AgricultureMenofia University University of the Chinese Academy of Sciences state key laboratory of plant Physiology and Biochemistry College of Biological SciencesChina Agricultural University state key laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural University

Salt stress is a major environmental factor limiting plant growth and productivity. We recently discovered an important new salt tolerance pathway, where the cell wall leucine-rich repeat extensins LRX3/4/5, the RAPID ALKALINIZATION FACTOR (RALF) peptides RALF22/23 and receptor-like kinase FERONIA(FER) function as a module to simultaneously regulate plant growth and salt stress tolerance. However, the intracellular signaling pathways that are regulated by the extracellular LRX3/4/5-RALF22/23-FER module to coordinate growth, cell wall integrity and salt stress responses are still unknown. Here, we report that the LRX3/4/5-RALF22/23-FER module negatively regulates the levels of jasmonic acid (JA), salicylic acid(SA) and abscisic acid (ABA). Blocking JA pathway rescues the dwarf phenotype of the lrx345 and fer-4mutants, while disruption of ABA biosynthesis suppresses the salt-hypersensitivity of these mutants. Many salt stress-responsive genes display abnormal expression patterns in the lrx345 and fer-4 mutants, as well as in the wild type plants treated with epigallocatechin gallate (EGCG), an inhibitor of pectin methylesterases,suggesting cell wall integrity as a critical factor that determines the expression pattern of stress-responsive genes. Production of reactive oxygen species (ROS) is constitutively increased in the lrx345 and fer-4mutants, and inhibition of ROS accumulation suppresses the salt-hypersensitivity of these ***, our work provides strong evidence that the LRX3/4/5-RALF22/23-FER module controls plant growth and salt stress responses by regulating hormonal homeostasis and ROS accumulation.

关键词： salt stress cell wall integrity growth LRX RALF FER hormone

Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Rice science 2017年第5期24卷 291-298页

作者： Manjesh SAAKRE Thirthikar Meera BABURAO Abida Puthenpeedikal SALIM Rose Mary FFANCIES Valasala Poothecty ACHUTHAN George THOMAS Sajeevan Radha SIVARAJAN Centre for plant biotechnology and Molecular Biology/department of genetics and plant breeding/department of agronomy College of Horticulture Kerala Agricultural University Vellanikkara Thrissur 680656 Kerala India

Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield *** and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought *** the present study,differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water,Pseudomonas fluorescens strain(Pf1) treated plants subjected for drought stress by withholding water and control(well-watered).Differentially expressed c DNAs of six genes(COX1,PKDP,b ZIP1,AP2-EREBP,Hsp20 and COC1) were identified,cloned and ***-time q PCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with *** revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive *** results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization,which might be a key element for induced systemic tolerance by microbes.

关键词： rice drought tolerance Pseudomonas fluorescens differential display reverse transcription polymerase chain reaction quantitative real-time PCR transcript derived fragment

Mechanism of plant immune activation and signaling: Insight from the first solved plant resistosome structure

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant Biology 2019年第8期61卷 902-907页

作者： Baomin Feng Dingzhong Tang state key laboratory of Ecological Control of Fujian-Taiwan Crop Pests Key Laboratory of Ministry of Education for Genetics Breeding and Multiple Utilization of Crops Plant Immunity Center Fujian Agriculture and Forestry University

Life or death?It’s a question animal and plant cells ask themselves during development and throughout their lives–and when they choose the latter option,cells can execute a genetically programmed set of cellular functions that result in cell *** animals and plants,the execution of programmed cell death is critical for proper development and for immunity against invading microbes.

关键词： genetically invading microbes

Effect of the suppression of BpAP1 on the expression of lignin related genes in birch

在线全文

学校读者我要写书评

暂无评论

Journal of Forestry Research 2022年第1期33卷 289-297页

作者： Haijiao Huang Shuo Wang Huiyu Li Jing Jiang state key laboratory of Tree genetics and breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China

Lignin is an integral part of secondary cell walls in plants and plays important roles in maintaining the strength of stems,enhancing transport ability of stems,and providing resistance to multiple *** biosynthesis has become one of the hotspots in molecular forest biology *** AP1 transcription factor plays important roles in plant fower ***,in this study,suppression of BpAP1 altered the transcription profiles of white birch and RNA-seq was used to find that suppression of BpAP1 changed the expression of lignin pathway-related genes;C4 H/CYP73A,POD were down-regulated and HCT,CCoAOMT,REF1 and CAD were *** walls of the suppressed transgenic birch were significantly thinner than the wild type of birch,and Bp AP1-repressed birch contained less *** addition to regulation of foral development,BpAP1 might play a role in regulating the expression of genes in lignin biosynthesis of *** study could provide a new insight into the function of AP1 genes in woody species.

关键词： BpAP1 Lignin biosynthesis RNA-seq Wood fiber cell walls

Prediction of VIGS efficiency by the Sfold program and its reliability analysis in Gossypium hirsutum

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2016年第7期61卷 543-551页

作者： Xiaoyang Ge Jie Wu Chaojun Zhang Qianhua Wang Yuxia Hou Zuoren Yang Zhaoen Yang Zhenzhen Xu Ye Wang Lili Lu Xueyan Zhang Jinping Hua Fuguang Li state key laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences department of plant genetics and breeding Key Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University College of Science China Agricultural University

Genetic transformation in some plant species, including cotton （Gossypium hirsutum）, is hampered by laborious and time-consuming processes and often unachievable. Virus-induced gene silencing （VIGS） by double-stranded RNAs can serve as a reverse-genetics tool to determine gene function. However, knockdown levels vary greatly when using a tobacco rattle virus-based vector that carries different cDNA fragments of a gene. How to choose the optional target fragment for high interference efficiency is very challenging. Addressing this challenge requires increasing the efficacy of small interference RNA （siRNA） in target fragment. Here, we describe a method to assess VIGS efficiency by comparing the following parameters of siRNA in target sequence： the disruptionenergy of the target （△Gdisruption）, the differential stability of siRNA duplex ends （DSSE）, and the internal stability at positions 9-14 of the siRNA antisense strand （AIS）, which are calculated by Sfold program （http：//***. org）. We find that the siRNAs with low mGdisruption, high DSSE and high AIS have high activity and easily result in high VIGS efficiency by experimentally testing the actual knockdown levels of the four target genes, GhPDS, GhCLA1, GhAOS1, and GhCXE1 via choosing different target sequences for each gene. Therefore, the Sfold pro- gram can be used to analyze target sequences when car- rying out VIGS design to increase gene-silencing effects in plants.

关键词： VIGS Sfold program siRNA Targetsequences Gossypium hirsutum