检索结果-南通市图书馆

Viroids:Small Probes for Exploring the Vast Universe of RNA Trafficking in plants

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative plant biology 2010年第1期52卷 28-39页

作者： Ying Wang Biao Ding department of plant cellular and molecular biology and plant biotechnology center The Ohio State University The center for RNA biology The Ohio State University molecular Cellular and DeVelopmental Biology Program The Ohio State University Columbus OH 43210 USA

Cell-to-cell and long-distance trafficking of RNA is a rapidly evolving frontier of integrative plant biology that broadly impacts studies on plant growth and development, spread of infectious agents and plant defense responses. The fundamental questions being pursued at the forefronts revolve around function, mechanism and evolution. In the present review, we will first use specific examples to illustrate the biological importance of cell-to-cell and long-distance trafficking of RNA. We then focus our discussion on research findings obtained using viroids that have advanced our understanding of the underlying mechanisms involved in RNA trafficking. We further use viroid examples to illustrate the great diversity of trafficking machinery evolved by plants, as well as the promise for new insights in the years ahead. Finally, we discuss the prospect of integrating findings from different experimental systems to achieve a systems-based understanding of RNA trafficking function, mechanism and evolution.

关键词： no English key word

Endocytic Trafficking towards the Vacuole Plays a Key Role in the Auxin Receptor SCF^TIR-Independent Mechanism of Lateral Root Formation in A. thaliana

在线全文

学校读者我要写书评

暂无评论

molecular plant 2012年第6期5卷 1195-1209页

作者： Patricio Perez-Henrique Natasha V. Raikhel Lorena Norambuena plant molecular biology Laboratory Department of Biology Faculty of Sciences University of Chile Santiago Chile Millenium Nucleus in plant Cell biotechnology Chile center for plant Cell biology and department of Botany and plant Sciences University of California Riverside CA 92521 USA

plants＇ developmental plasticity plays a pivotal role in responding to environmental conditions. One of the most plastic plant organs is the root system. Different environmental stimuli such as nutrients and water deficiency may induce lateral root formation to compensate for a low level of water and/or nutrients. It has been shown that the hor- mone auxin tunes lateral root development and components for its signaling pathway have been identified. Using chemi- cal biology, we discovered an Arabidopsis thaliana lateral root formation mechanism that is independent of the auxin receptor SCFTM. The bioactive compound Sortin2 increased lateral root occurrence by acting upstream from the morpho- logical marker of lateral root primordium formation, the mitotic activity. The compound did not display auxin activity. At the cellular level, Sortin2 accelerated endosomal trafficking, resulting in increased trafficking of plasma membrane recy- cling proteins to the vacuole. Sortin2 affected Late endosome/PVC/MVB trafficking and morphology. Combining Sortin2 with well-known drugs showed that endocytic trafficking of Late E/PVC/MVB towards the vacuole is pivotal for Sortin2- induced SCFTm-independent lateral root initiation. Our results revealed a distinctive role for endosomal trafficking in the promotion of lateral root formation via a process that does not rely on the auxin receptor complex SCFTM.

关键词： lateral root development endocytic trafficking Sortin2 endosomes Arabidopsis.

Control of Directional Macromolecular Trafficking Across Specific cellular Boundaries:A Key to Integrative plant biology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant biology 2007年第8期49卷 1227-1234页

作者： BiaoDing AsukaItaya department of plant cellular and molecular biology and plant biotechnology center Ohio State University ColumbusOhio 43210USA The OSU RNA GroupOhio State UniversityColumbusOhio 43210USA MolecularCellular and Developmental Biology ProgramOhio State UniversityColumbusOhio 43210USA

There is now solid evidence that cell-to-cell trafficking of certain proteins and RNAs plays a critical role in trans-cellular regulation of gene expression to coordinate cellular differentiation and development. Such trafficking also is critical for viral infection and plant defense. The mechanisms of trafficking remain poorly understood. Although some proteins may move between cells by diffusion, many proteins and RNAs move in a highly regulated fashion. Regulation is likely achieved through interactions between distinct protein or RNA motifs and cellular factors. Some motifs and factors have been identified. One of the major focuses for future studies is to identify all motifs and their cognate factors and further elucidate their roles in trafficking between specific cells. With increasing information from such studies, we should be able to develop an understanding of the mechanisms that regulate trafficking of various proteins and RNAs across all and specific cellular boundaries. On the basis of such mechanistic knowledge, we can further investigate how the trafficking machinery has evolved to regulate developmental and physiological processes in a plant, how pathogens have co-evolved to use this machinery for systemic spread in a plant, and how plants use this machinery for counterdefense.

关键词： CAPRICE GLABRA3 KNOTTED-I phloem plasmodesmata protein trafficking RNA trafficking SHORT-ROOT viral movementprotein viroid

The regeneration factors ERF114 and ERF115 regulate auxin-mediated lateral root development in response to mechanical cues

在线全文

学校读者我要写书评

暂无评论

molecular plant 2022年第10期15卷 1543-1557页

作者： Balkan Canher Fien Lanssens Ai Zhang Anchal Bisht Shamik Mazumdar Jefri Heyman Sebastian Wolf Charles W.Melnyk Lieven De Veylder department of plant biotechnology and Bioinformatics Ghent UniversityGhent B-9052Belgium VIB center for plant Systems biology Ghent B-9052Belgium department of plant biology Linnean Center for Plant BiologySwedish University of Agricultural SciencesAlmas alle 575651 UppsalaSweden department of plant Biochemistry Center for Plant Molecular Biology(ZMBP)University of Tubingen72076 TubingenGermany

plants show an unparalleled regenerative capacity,allowing them to survive severe stress conditions,such as injury,herbivory attack,and harsh weather *** potential not only replenishes tissues and restores damaged organs but can also give rise to whole plant *** the intertwined nature of development and regeneration,common upstream cues and signaling mechanisms are largely ***,we demonstrate that in addition to being activators of regeneration,ETHYLENE RESPONSE FACTOR 114(ERF114)and ERF115 govern developmental growth in the absence of wounding or *** ERF114 and ERF115 activity enhances auxin sensitivity,which is correlated with enhanced xylem maturation and lateral root formation,whereas their knockout results in a decrease in lateral ***,we provide evidence that mechanical cues contribute to ERF114 and ERF115 expression in correlation with BZR1-mediated brassinosteroid signaling under both regenerative and developmental ***,cell wall integrity surveillance via mechanosensory FERONIA signaling suppresses their expression under both *** together,our data suggest a molecular framework in which cell wall signals and mechanical strains regulate organ development and regenerative responses via ERF114-and ERF115-mediated auxin signaling.

关键词： lateral root ERF114 mechanical stress cell wall integrity FERONIA auxin

The Formation of Anthocyanic Vacuolar Inclusions in Arabidopsis thaliana and Implications for the Sequestration of Anthocyanin Pigments

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

molecular plant 2010年第1期3卷 78-90页

作者： Lucille Pourcel Niloufer G. Irani Yuhua LU Ken Riedl Steve Schwartz Erich Grotewold department of plant cellular and molecular biology and plant biotechnology center Ohio State University Columbus OH 43210 USA department of Food Science and Technology The Ohio State University Columbus OH 43210 USA Present address: department of plant Systems biology Flanders Institute for Biotechnology and Department of Plant Biotechnology and Genetics GhentUniversity 9052 Ghent Belgium Current address: department of Chemistry Eastern Illinois University Charleston IL 61920 USA

Anthocyanins are flavonoid pigments that accumulate in the large central vacuole of most plants. Inside the vacuole, anthocyanins can be found uniformly distributed or as part of sub-vacuolar pigment bodies, the Anthocyanic Vacuolar Inclusions （AVIs）. Using Arabidopsis seedlings grown under anthocyanin-inductive conditions as a model to un- derstand how AVIs are formed, we show here that the accumulation of AVIs strongly correlates with the formation of cyanidin 3-glucoside （C3G） and derivatives. Arabidopsis mutants that fail to glycosylate anthocyanidins at the 5-0 position （Sgt mutant） accumulate AVIs in almost every epidermal cell of the cotyledons, as compared to wild-type seedlings, where only a small fraction of the cells show AVIs. A similar phenomenon is observed when seedlings are treated with vanadate. Highlighting a role for autophagy in the formation of the AVIs, we show that various mutants that interfere with the autophagic process （atg mutants） display lower numbers of AVIs, in addition to a reduced accumulation of anthocyanins. Interestingly, vanadate increases the numbers of AVIs in the atg mutants, suggesting that several pathways might participate in AVl formation. Taken together, our results suggest novel mechanisms for the formation of sub-vacuolar compartments capable of accumulating anthocyanin pigments.

关键词： Anthocyanin autophagy cyanidin 3-glucoside vacuolar inclusion vanadate.

Sensory sacrifices when we mass-produce mass produce

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2016年第1期3卷 167-172页

作者： Kevin M Folta Harry J Klee Horticultural Sciences department Plant Innovation Center and The Graduate Program for Plant Molecular and Cellular BiologyUniversity of FloridaGainesville32606FLUSA

plant breeders have been extremely successful at driving genetic improvements in ***,‘improvements’are truly a question of *** the last one-hundred years most plant genetic innovations have been driven by industry *** fruits,heavier yields,uniformity,increased resistance to disease and better shipping quality are just a few of the traits that have ensured profits on the farm and affordable food for ***,these milestones have come at the expense of sensory qualities,which have been sacrificed in exchange for practical production *** a base of industry-sufficient genetics,today’s breeders can now turn to the consumer for guidance in defining critical *** approaches to plant breeding start with the analysis of consumer preferences,and then merge them with modern genomics and analytical chemistry *** result is the next generation of crops that meet supply chain demands while presenting improvements in flavor,nutrition,color,aroma and *** review analyzes the approach of consumer-assisted selection as it has been applied to tomato and strawberry,two complementary annual crops that have been intensively bred to meet industry *** breeding efforts start with the consumer,with the objective of reclaiming lost sensory qualities.

关键词： crops breeding driving

Epigenetic Variations in plant Hybrids and Their Potential Roles in Heterosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2013年第5期40卷 205-210页

作者： Guangming He Hang He Xing Wang Deng Peking-Yale Joint center for plant molecular Genetics and Agro-biotechnology State Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking University department of molecular Cellularand Developmental BiologyYale UniversityNew HavenCT 06520USA

Heterosis,one of the most important biological phenomena, refers to the phenotypic superiority of a hybrid over its genetically diverse parents with respect to many traits such as biomass,growth rate and *** its successful application in breeding and agronomic production of many crop and animal varieties,the molecular basis of heterosis remains *** classic genetic explanations for heterosis centered on three hypotheses：dominance（Davenport,1908;Bruce,

关键词： DNA siRNAs DCL gene

The Development of Protein Microarrays and Their Applications in DNA-Protein and Protein-Protein Interaction Analyses of Arabidopsis Transcription Factors

在线全文

学校读者我要写书评

暂无评论

molecular plant 2008年第1期1卷 27-41页

作者： Wei Gong Kun He Mike Covington S. R Dinesh-Kumar Michael Snyder Stacey L. Harmer Yu-Xian Zhu Xing Wang Denga Peking-Yale Joint center for plant molecular Genetics and Agrobiotechnology Co and Plant Genetic Engineering Peking University Beijing 100871 China department of molecular Cellular and Developmental Biology Yale University New Haven Connecticut 06520 USA Section of plant biology College of Biological Sciences University of California Davis 95616 USA

We used our collection of Arabidopsis transcription factor （TF） ORFeome clones to construct protein microarrays containing as many as 802 TF proteins. These protein microarrays were used for both protein-DNA and proteinprotein interaction analyses. For protein-DNA interaction studies, we examined AP2/ERF family TFs and their cognate cis-elements. By careful comparison of the DNA-binding specificity of 13 TFs on the protein microarray with previous non-microarray data, we showed that protein microarrays provide an efficient and high throughput tool for genome-wide analysis of TF-DNA interactions. This microarray protein-DNA interaction analysis allowed us to derive a comprehensive view of DNA-binding profiles of AP2/ERF family proteins in Arabidopsis. It also revealed four TFs that bound the EE （evening element） and had the expected phased gene expression under clock-regulation, thus providing a basis for further functional analysis of their roles in clock regulation of gene expression. We also developed procedures for detecting protein interactions using this TF protein microarray and discovered four novel partners that interact with HY5, which can be validated by yeast two-hybrid assays. Thus, plant TF protein microarrays offer an attractive high-throughput alternative to traditional techniques for TF functional characterization on a global scale.

关键词： transcription factor (TF) TF-DNA EE (evening element)

Gibberellin Signal Transduction in Rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative plant biology 2007年第6期49卷 731-741页

作者： Liu-Min Fan Xiaoyan Feng Yu Wang Xing Wang Deng Peking-Yale Joint center of plant molecular Genetics and Agro-biotechnology National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China department of molecular Cellular and Developmental Biology Yale University New Haven Connecticut 06520-8104 USA

In the past decade, significant knowledge has accumulated regarding gibberellin （GA） signal transductlon In rice as a result of studies using multiple approaches, particularly molecular genetics. The present review highlights the recent developments In the identification of GA signaling pathway components, the discovery of GA-Induced destructlon of GA signaling repressor （DELLA protein）, and the possible mechanism underlying the regulation of GA- responsive gene expression in rice.

关键词： gene expression gibberellin protein degradation rice signal transduction.