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Observation on Effect of Gennlanchun (更年春) In Preventing Experimental Primary Osteoporosis

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chinese Journal of Integrated traditional and Western medicine 2003年第2期9卷 127-127页

作者：王文君俞瑾王静周光兴 Obstetric and Gynecology hospital Fudan UniversityShanghai(200011) department of Pathology Section of Experimental AnimalsFudan University

Objective: To evaluate the efficacy of Genni-anchun (GNC) in preventing primary osteoporosis. Methods: Fifty female SD rats of 10 - 12 months old were randomly divided into five groups. Except those in the sham operation group, animals were ovariectomized bilaterally. The gastric perfusion was started immediately after operation, with normal saline to the sham operation group and model group; GNC to high dose (2 ml) and low dos (1 ml) GNC groups and Nilestriol to the NE group, every day for 3 months. Animals were sacrificed at the end of experiment, their vertebra and

关键词： were Observation on Effect of Gennlanchun in GNC of In Preventing Experimental Primary Osteoporosis

CGD-1, a defensin-related peptide derived from marine chinese medicine Ostreae concha, inhibits the Gram-negative bacteria by membrane attack

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TMR Pharmacology Research 2023年第2期3卷 38-45页

作者： Wei-Tao Wang Qing-Zhou Meng Long-Fen Li Wei-Yi Meng Xin Yin Pei-Pei Zhao Meng-Meng Wang Jing-Li Ren Xue-Kui Xia Chun-Lei Li department of pharmacy Qilu Hospital of Shandong UniversityJinan 250001China Biology Institute Qilu University of Technology(Shandong Academy of Sciences)Jinan 250021China

There is an increasing interest in discovering new antibacterial agents derived from nature to enhance the treatment of various bacterial *** and their derived peptide fragments exhibit significant antibacterial activity without any cytotoxic effects,making them attractive features for potential novel antibacterial *** gigas,a traditional seafood that has been used worldwide for centuries,has its shells applied in chinese medicine as Ostreae *** this study,bioinformatics analysis was used to obtain a novel antibacterial peptide,CGD-1,derived from marine chinese medicine Ostreae *** physicochemical characterization and circular dichroism analysis results demonstrated that CGD-1 assembled into anα-helical structure in a simulated membrane environment,and it displayed antibacterial action against Gram-negative *** minimal inhibitory concentrations against both Pseudomonas aeruginosa ATCC27853 and Escherichia coli ATCC25922 were 25μ***-1 was able to efficiently permeate the cell *** in bacterial cell morphology were evaluated using a field emission scanning electron *** results suggested that CGD-1 exerted its antibacterial activity through permeabilizing and disrupting the bacterial cell ***,CGD-1 may have potential applications in fighting against pathogenic bacteria such as *** and ***.

关键词： defensin antibacterial activity hemolymph Crassostrea gigas Ostreae concha

Preparation and in vitro Evaluation of Salidroside Nanoparticle

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Medicinal Plant 2022年第2期13卷 12-15页

作者： Yue FENG Wei ZOU Mengran GUO Xing LAN Lili HE College of pharmacy Southwest Minzu University Chengdu 610041 China Key Frontiers Science Center for Disease-related Molecular Network State Key Laboratory of Biotherapy and Cancer Center Department of Clinical Pharmacy West China Hospital Sichuan University Chengdu 610041 China

[Objectives]To use poly(lactic-co-glycolic acid)(PLGA)nanoparticles to improve the bioavailability and brain entrance capability of Salidroside(Sal).[Methods]An emulsion solvent evaporation approach was used to create PLGA nanoparticles(Sal-NP).The preparation parameters were optimized using a single factor *** particle size and zeta potential were determined using the laser particle analyzer,and the morphology of the nanoparticles was observed using transmission electron *** encapsulation efficiency and drug loading were determined using ***,the in vitro drug release was determined using a dynamic dialysis method,and the cellular uptake and cytotoxicity were determined using the bEnd3 cell model.[Results]The ultrasonic time and power for preparing Sal-NP were 6 min and 100 W,*** size of the nanoparticles was 162.0±74.86 nm,and the morphology of Sal-NP was spherical *** 48 h,the cumulative release of Sal-NP was 62%,indicating that Sal showed a controlled release property in *** uptake study showed that the PLGA nanoparticles remarkably increased the internalization than control group(P<0.001).In addition,Sal-NPs were non-toxic to cells at concentrations ranging from 12.5 to 100μM.[Conclusions]PLGA nanoparticles is promising to be exploited in Alzheimer's disease research due to the increasing absorption and controlled release advantages for Sal.

关键词： Salidroside Polylactic acid-glycolic acid copolymer Nanoparticles Controlled release Alzheimer's disease

针灸联合康复治疗对脊髓损伤患者临床疗效、神经功能恢复及神经细胞因子的影响

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四川中医 2022年第5期40卷 204-208页

作者：高辰班妮娅·巴合提郝培如李晓静 School of pharmacy and health Creighton University68178 新疆医科大学第四临床医学院新疆乌鲁木齐830000 乌鲁木齐市中医医院康复科新疆乌鲁木齐830000 杭州养和医院康复科浙江杭州310000

目的:探讨中医针灸联合康复治疗对脊髓损伤患者临床疗效、神经功能恢复及神经细胞因子的影响。方法:2017年3月~2020年3月本院收治的90例脊髓损伤患者,根据治疗方法不同分为2组(康复治疗组及中医针灸联合康复治疗组,各45例)。对比2组临... 详细信息

目的:探讨中医针灸联合康复治疗对脊髓损伤患者临床疗效、神经功能恢复及神经细胞因子的影响。方法:2017年3月~2020年3月本院收治的90例脊髓损伤患者,根据治疗方法不同分为2组(康复治疗组及中医针灸联合康复治疗组,各45例)。对比2组临床疗效、神经功能恢复情况及神经细胞因子变化水平,并分析2种治疗方案对患者生活质量的影响。结果:(1)联合治疗组患者治疗后总有效率95.56%显著高于康复治疗组80.00%(P<0.05)。(2)治疗后,两组患者ASIA评分均升高,联合治疗组患者ASIA评分显著高于康复治疗组患者(P<0.05)。(3)治疗后,两组患者IL-1β、IL-5、TNF-α水平均明显降低,联合治疗组患者明显低于康复治疗组患者(P<0.05)。(4)治疗后,两组患者WHOQOL-100评分(心理、生理、环境、社会关系)各维度评分均明显升高,联合治疗组患者WHOQOL-100评分各维度评分明显高于康复治疗组患者(P<0.05)。结论:中医针灸治疗联合康复训练可促进患者神经功能恢复、抑制神经细胞因子的释放,并有效改善了其生活质量,对促进患者康复有重要意义。

关键词：中医针灸康复治疗脊髓损伤神经功能

低氧诱导因子的分子调控机制及中药的干预作用

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中国中药杂志 2020年第20期45卷 4805-4811页

作者：刘不悔王美子孙伟万毅刚吴薇房其军王文文 YEE Hong-yun 袁灿灿刘莹露涂玥 CHONG Fee-lan 南京中医药大学附属医院肾内科江苏南京210029 南京中医药大学中西医结合鼓楼临床医学院中医科江苏南京210008 南京大学医学院附属鼓楼医院中医科江苏南京210008 南京中医药大学针灸推拿学院·养生康复学院中医养生学教研室江苏南京210023 The School of pharmacy Management and Science UniversityShah Alam 40100Malaysia

低氧诱导因子(hypoxia-inducible factors,HIFs)是机体内感受和调节细胞氧浓度的关键转录因子。HIF-1由α和β等2个亚基组成,其中,HIF-1α的分子调控机制涉及其降解和活化等主要过程。HIF-1α的降解是由氧依赖性信号途径调控的,包括"希... 详细信息

低氧诱导因子(hypoxia-inducible factors,HIFs)是机体内感受和调节细胞氧浓度的关键转录因子。HIF-1由α和β等2个亚基组成,其中,HIF-1α的分子调控机制涉及其降解和活化等主要过程。HIF-1α的降解是由氧依赖性信号途径调控的,包括"希佩尔-林道病恶性肿瘤抑制蛋白(von hippel-lindau protein,pVHL)依赖通路"和"pVHL非依赖性通路";而HIF-1α的活化是由氧非依赖性信号途径调控的,包括哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)/真核生物始动因子4E结合蛋白1(eukaryotic initiation factor 4E-binding protein 1, 4EBP1)/HIF-1α通路、磷脂酰肌醇3激酶(phosph atidylinositol 3-kinase,PI3K)/丝氨酸-苏氨酸蛋白激酶(proteirrserinc-threonine kinases,Akt)/HIF-1α通路以及沉默信息调节因子1(silent information regulator1,Sirt1)/HIF-1α通路等。近年来,基于HIFs的分子调控机制,不仅研制出治疗肾性贫血的新药罗沙司他,在中药提取物中也发现了一些药理作用类似于HIFs的大分子物质,如大黄素、三七皂苷、厚朴酚以及灵仙新苷等,这些天然的大分子物质在炎症反应、自噬、表观遗传修饰以及细胞焦亡中发挥调控性作用。值得注意的是,对于常见的低氧相关疾病,如糖尿病肾病,HIFs介导的"细胞焦亡"可能是清利湿热类中药及其代表性经方(如二妙丸)干预炎症性组织、细胞损伤的新靶点。

关键词：低氧诱导因子中药分子机制信号通路细胞焦亡

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模拟失重不同时长对大鼠抑郁样行为的影响

生物化学与生物物理进展 2023年第2期50卷 346-357页

作者：张米佳王孟迪 Ayaz Ahmed 董丽姚勤伟李莹辉曲丽娜陈善广刘新民王琼西南医科大学附属中医医院中葡中医药国际合作中心泸州646000 中国农业科学院农产品加工研究所北京100193 西南医科大学药学院泸州646000 首都医科大学附属北京佑安医院重症肝病科北京100069 中国航天员科研训练中心航天医学基础与应用国家重点实验室北京100094 中国航天员科研训练中心人因工程国家重点实验室北京100094 中国医学科学院北京协和医学院药用植物研究所药理毒理研究中心北京100193 Dr.Panjwani Center for Molecular medicine and Drug Research International Center for Chemical and Biological SciencesUniversity of KarachiKarachi 75270Pakistan

目的本研究旨在观察不同持续时间的模拟失重对大鼠抑郁样行为和海马超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的影响,以探究其影响及可能的作用机制。方法采用后肢悬挂(HLS)尾吊法模拟大鼠失重状态。将大鼠分为对照组和不同模拟失重时间尾... 详细信息

目的本研究旨在观察不同持续时间的模拟失重对大鼠抑郁样行为和海马超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的影响,以探究其影响及可能的作用机制。方法采用后肢悬挂(HLS)尾吊法模拟大鼠失重状态。将大鼠分为对照组和不同模拟失重时间尾吊组(尾吊时长分别为1、2、3、4周)。采用旷场实验(OFT)、新物体识别实验(NORT)、强迫游泳实验(FST)观察大鼠抑郁样行为,采用酶联免疫吸附试验(ELISA)法测定海马SOD和CAT活性。结果OFT结果显示,与对照组相比,HLS不同时间大鼠的僵滞时间增加(P<0.05,P<0.01)。在NORT中,与对照组相比,HLS不同时间大鼠对新物体的探索潜伏期增加,探索次数和时间减少(P<0.05,P<0.01)。在FST中,与对照组相比,HLS不同时间大鼠在FST中的不动时间增加,攀爬次数减少(P<0.05,P<0.01)。与对照组相比,HLS不同时间组大鼠海马组织中SOD和CAT水平均下降(P<0.05,P<0.01)。结论短时间或长时间的失重都会导致大鼠产生类抑郁样行为。

关键词：模拟失重抑郁样行为旷场实验强迫游泳实验超氧化物歧化酶过氧化氢酶

Clinical Observation and Safety Evaluation of Zhuang medicine Xiaoyan Zhiyang Formula for Dermatosis

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Medicinal Plant 2023年第6期14卷 43-46页

作者： Wen ZHONG Xixi QI Jiangcun WEI Xian PENG Fangmei LI Guangxi International Zhuang Medical hospital Nanning 530201China

[Objectives]To observe the clinical efficacy and safety of Zhuang medicine Xiaoyan Zhiyang Formula by retrospective analysis of clinical use.[Methods]A total of 225 cases were collected,including 117 males and 108 females,115 cases of contact dermatitis and 110 cases of acute *** medicine Xiaoyan Zhiyang Formula was administered to 225 patients with contact dermatitis and acute eczema and the clinical efficacy was evaluated.[Results]After one week of treatment,among 225 patients with contact dermatitis and acute eczema,76 cases were cured(33.78%),133 cases showed marked response(59.11%),16 cases(7.11%)were improved,0 case was ineffective,and the overall response rate was 92.89%.After one week of Zhuang medicine Xiaoyan Zhiyang Formula treatment,the symptoms of itching,pain and swelling were significantly improved in all patients,and the rash was partially crusted without new symptom,and there were no complications and adverse reactions.[Conclusions]Zhuang medicine Xiaoyan Zhiyang Formula therapy has a remarkable effect in the treatment of contact dermatitis and acute eczema.

关键词： Zhuang medicine Xiaoyan Zhiyang Formula Contact dermatitis Acute eczema Clinical drug use Safety evaluation

基于自噬信号通路探究褐藻多糖硫酸酯改善人近端肾小管上皮细胞衰老的分子机制

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中国中药杂志 2020年第24期45卷 6003-6011页

作者：房其军刘建璟万毅刚刘不悔涂玥吴薇刘莹露王文文王美子 YEE Hong-yun 袁灿灿 CHONG Fee-lan 南京中医药大学中西医结合鼓楼临床医学院中医科江苏南京210029 江苏省中西医结合医院肾内科江苏南京210028 南京大学医学院附属鼓楼医院中医科江苏南京210008 南京中医药大学附属医院肾内科江苏南京210029 南京中医药大学针灸推拿学院•养生康复学院中医养生学教研室江苏南京210023 School of pharmacy Management and Science UniversityShah Alam 40100

褐藻多糖硫酸酯(fucoidan,FPS)是临床上治疗慢性肾衰竭中成药海昆肾喜胶囊的有效成分,具有潜在的抗衰老作用。然而,FPS是否能改善肾脏衰老,尤其是对于其抗衰老的分子机制,目前仍不清楚。将体外培养的人近端肾小管上皮细胞(HK-2)分为正常... 详细信息

褐藻多糖硫酸酯(fucoidan,FPS)是临床上治疗慢性肾衰竭中成药海昆肾喜胶囊的有效成分,具有潜在的抗衰老作用。然而,FPS是否能改善肾脏衰老,尤其是对于其抗衰老的分子机制,目前仍不清楚。将体外培养的人近端肾小管上皮细胞(HK-2)分为正常组(normal group,N)、模型组(D-gal model group,D)、低剂量FPS组(low dose of FPS,L-FPS)、高剂量FPS组(high dose of FPS,H-FPS)以及维生素E组(vitamin E,VE),分别进行不同的干预。N组加入1%胎牛血清(fetal bovine serum,FBS)1 mL;D组加入75 mmol·L^(-1)D-gal;L-FPS组加入75 mmol·L^(-1)D-gal+25μg·mL^(-1)FPS;H-FPS组加入75 mmol·L^(-1)D-gal+50μg·mL^(-1)FPS;VE组加入75 mmol·L^(-1)D-gal+50μg·mL^(-1)VE。在干预后24 h,首先,观察D-gal对HK-2细胞β-半乳糖苷酶(senescence-associatedβ-galactosidase,SA-β-gal)染色特征以及klotho,P53,P21蛋白表达水平和腺苷酸活化蛋白激酶(adenosine monophosphate activated protein kinase,AMPK)-不协调的51类激酶1(uncoordinated 51-like kinase 1,ULK1)信号通路活性的影响;其次,观察FPS和VE对D-gal诱导的HK-2细胞SA-β-gal染色特征以及klotho,P53,P21蛋白表达水平的影响;最后,观察FPS和VE对D-gal诱导的HK-2细胞哺乳动物同族物微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)蛋白表达水平以及AMPK-ULK1信号通路活性的影响。结果表明,对于HK-2细胞,75 mmol·L^(-1)的D-gal能安全而有效地诱导细胞SA-β-gal染色特征和klotho,P53,P21蛋白表达水平的变化,也就是引发细胞衰老;而FPS和VE都能改善D-gal诱导的细胞SA-β-gal染色特征以及klotho,P21,P53蛋白表达水平的变化,也就是拮抗细胞衰老,这里,两者的作用是类似的;D-gal不仅能诱导细胞衰老,还能促进LC3Ⅱ,磷酸化AMPK(phosphorylated-AMPK,p-AMPK)以及磷酸化ULK1(phosphorylated-ULK1,p-ULK1)蛋白高表达,激活自噬相关AMPK-ULK1信号通路;而FPS和VE都能改善D-gal诱导的细胞LC3Ⅱ,p-AMPK以及p-ULK1蛋白表达水平的变化,也就是抑制自噬相关AMPK-ULK1信号通路活性。总之,对于D-gal诱导的人近端肾小管上皮细胞衰老模型,FPS与VE相似,能改善肾脏细胞衰老,其分子机制可能与抑制自噬相关AMPK-ULK1信号通路活性有关。这一发现为FPS抗肾脏衰老提供了初步的药理学证据。

关键词：衰老褐藻多糖硫酸酯人近端肾小管上皮细胞自噬 AMPK-ULK1信号通路

水动力尾静脉注射介导Akt/β-catenin/Yap质粒转染建立小鼠肝癌模型

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临床与实验病理学杂志 2020年第10期36卷 1185-1190页

作者：蒋雨薇宋海燕李怡萍汤凯悦徐娇雅 SULAIMAN Andrew MCGARRY Sarah WANG Lisheng 郑培永上海中医药大学附属龙华医院脾胃病研究所上海200032 中加联合脾胃病研究中心上海200032 department of Biochemistry Microbiology and ImmunologyUniversity of OttawaOttawaON K1H8M5Canada

目的通过应用水动力尾静脉注射(hydrodynamic tail vein injection,HTVi)Sleeping Beauty(SB)质粒及促癌基因质粒,构建一种新型小鼠肝癌模型。方法6周龄雄性C57BL/6J小鼠,按照体重随机分为对照组和模型组。将冻存的SB、Akt、β-catenin... 详细信息

目的通过应用水动力尾静脉注射(hydrodynamic tail vein injection,HTVi)Sleeping Beauty(SB)质粒及促癌基因质粒,构建一种新型小鼠肝癌模型。方法6周龄雄性C57BL/6J小鼠,按照体重随机分为对照组和模型组。将冻存的SB、Akt、β-catenin、Yap质粒转化入感受态*** DH5α大肠杆菌,于LB培养基扩增后提取质粒。每只模型组小鼠尾静脉快速注射上述质粒混合溶液2 mL,包含SB质粒5μg,Akt、β-catenin、Yap质粒分别15μg,对照组小鼠给予等量PBS尾静脉注射。继续饲养8周后处死小鼠,观察小鼠情况,记录体重、肝重。观察小鼠肝脏病理学变化,并应用Western blot法检测小鼠肝组织中Akt、β-catenin、Yap基因表达水平。结果高压注射质粒8周后,对照组小鼠活动度好,皮毛光亮;小鼠肝脏形态正常;肝组织HE染色显示,对照组小鼠肝小叶结构完整。模型组小鼠活动减少,皮毛无光泽。与对照组比较,模型组小鼠体重下降、肝重增加、肝体比显著上调;模型组小鼠肝脏体积增大,质地较硬,表面出现颗粒状、结节样改变;肝组织HE染色显示,模型组小鼠肝组织结构紊乱,并出现多个肿瘤结节。此外,与对照组相比,模型组小鼠肝组织中Akt、β-catenin、Yap基因表达均明显上调。结论应用水动力尾静脉注射SB质粒及Akt/β-catenin/Yap质粒的方法可成功建立小鼠肝癌模型,为肝癌发病机制及药物研发提供快速、经济的动物模型。

关键词：肝肿瘤动物模型水动力尾静脉注射 SB质粒癌基因