检索结果-南通市图书馆

Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro

International Journal of Oral Science 2018年第2期10卷 117-126页

作者： Sema S.Hakki S.Buket Bozkurt Emre Türkay Michel Dard Nuhan Purali Werner Gotz department of periodontology Faculty of DentistrySelcuk University Research Center of Dental Faculty Selcuk University department of Orthodontics Oral Biology LaboratoryFaculty of DentistryUniversity of Bonn department of periodontology and implant dentistry College of DentistryNew York University department of Biophysics Faculty of MedicineHacettepe University

Amelogenin（AMG） is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG（rhAMG） on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts（OCCM-30）were treated with different concentrations（0.1, 1, 10, 100, 1 000, 10 000, 100 000 ng · mL^-1） of recombinant human AMG（rhAMG）and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein（BSP）, osteocalcin（OCN）, collagen type I（COL I）, osteopontin（OPN）, runt-related transcription factor 2（Runx2）, cementum attachment protein（CAP）, and alkaline phosphatase（ALP） genes using quantitative RT-PCR. The dose response of rhAMG was evaluated using a real-time cell *** RNA was isolated on day 3, and cell mineralization was assessed using von Kossa staining on day 8. COL I, OPN and lysosomalassociated membrane protein-1（LAMP-1）, which is a cell surface binding site for amelogenin, were evaluated using immunocytochemistry. F-actin bundles were imaged using confocal microscopy. rhAMG at a concentration of 100 000 ng · mL^-1 increased cell proliferation after 72 h compared to the other concentrations and the untreated control group. rhAMG（100 000 ng · mL^-1） upregulated BSP and OCN mRNA expression levels eightfold and fivefold, respectively. rhAMG at a concentration of 100 000 ng · mL^-1 remarkably enhanced LAMP-1 staining in cementoblasts. Increased numbers of mineralized nodules were observed at concentrations of 10 000 and 100 000 ng · mL^-1 rhAMG. The present data suggest that rhAMG is a potent regulator of gene expression in cementoblasts and support the potential application of rhAMG in therapies aimed at fast regeneration of damaged periodontal tissue.

关键词： AMG Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro OCN

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluation of periodontal healing in class II furcation defects following guided tissue regeneration with two different types of poly lactic acid membranes

引用

Chinese Medical Journal 2004年第2期17卷 270-274页

作者： Deniz Cetiner Berrinnsal Ates Parlar ElifGültekin BülentKurtis department of periodontology Faculty of dentistry Gazi University Ankara TurkeyDepartment of Periodontology Faculty of Dentistry Gazi University Ankara TurkeyDepartment of Periodontology Faculty of Dentistry Gazi University Ankara TurkeyDepartment of Pathology Faculty of Dentistry Gazi University Ankara TurkeyDepartment of Periodontology Faculty of Dentistry Gazi University Ankara Turkey

Background Guided tissue regeneration procedures provide predictable reconstruction of periodontal tissues in the treatment of furcation involvements in animals and huamns. This study was to compare long-term effectiveness of two different types of polylactic acid (PLA) membranes on periodontal regeneration in surgically created class II furcation defects in dogs.Methods Full thickness mucoperiosteal flap was raised on the buccal aspects of the experimental teeth and class II furcation defects having 5 mm vertical dimensions were created on mandibular premolar III and IV on each quadrant. The exposed root surfaces were thoroughly planed and PLA membranes were placed over the experimental defects on both sites. One site received liquid polymer membrane (LPM), and resorbable periodontal mesh (RPM) membranes were applied to the other site. The animals were sacrificed at 7 months after surgery and the specimens were processed for histological evaluation.Results The average length of new attachment formed on the treated roots in both groups ranged from 3. 02 mm to 4. 5 mm. Complete bone filling was observed at the furcation sites. No statistically significant differences were found between two membranes in any of the parameters (P>0. 05).Conclusion This study demonstrates favorable regenerative outcomes by the use of two different types of PLA membranes that could be used as alternatives for guided tissue regeneration (GTR).

关键词： guided tissue regeneration polyfactic acid membranes furcation/surgery experimental studies

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Juxtacrine interaction of macrophages and bone marrow stromal cells induce interleukin-6 signals and promote cell migration

引用

Bone Research 2015年第2期3卷 100-111页

作者： Jia Chang Amy J Koh Hernan Roca Laurie K McCauley department of Periodontics and Oral Medicine University of Michigan School of Dentistry department of periodontology University of Florida College of Dentistry department of Pathology University of Michigan Medical School

The bone marrow contains a heterogeneous milieu of cells, including macrophages, which are key cellular mediators for resolving infection and inflammation. Macrophages are most well known for their ability to phagocytose foreign bodies or apoptotic cells to maintain homeostasis; however, little is known about their function in the bone microenvironment. In the current study, we investigated the in vitro interaction of murine macrophages and bone marrow stromal cells （BMSCs）, with focus on the juxtacrine induction of IL-6 signaling and the resultant effect on BMSC migration and growth. The juxtacrine interaction of primary mouse macrophages and BMSCs activated IL-6 signaling in the co-cultures, which subsequently enhanced BMSC migration and increased BMSC numbers. BMSCs and macrophages harvested from IL-6 knockout mice revealed that IL-6 signaling was essential for enhancement of BMSC migration and increased BMSC numbers via juxtacrine interactions. BMSCs were the main contributor of IL-6 signaling~ and hence activation of the IL-6/ gp130/STAT3 pathway. Meanwhile, macrophage derived IL-6 remained important for the overall production of IL-6 protein in the co-cultures. Taken together, these findings show the function of macmphages as co-inducers of migration and growth of BMSCs, which could directly influence bone formation and turnover.

关键词： BMSCs IL bone STAT cell

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Caffeic acid phenethyl ester protects against oxidative stress and dampens inflammation via heme oxygenase 1

引用

International Journal of Oral Science 2019年第1期11卷 50-57页

作者： Alexandra St?hli Ceeneena Ubaidha Maheen Franz Josef Strauss Sigrun Eick Anton Sculean Reinhard Gruber department of Oral Biology School of Dentistry Medical University of Vienna department of periodontology School of Dental MedicineUniversity of Bern department of Conservative dentistry School of Dentistry University of Chile Austrian Cluster for Tissue Regeneration

Periodontal disease is associated with chronic oxidative stress and inflammation. Caffeic acid phenethyl ester(CAPE), which is a potent inducer of heme oxygenase 1(HO1), is a central active component of propolis, and the application of propolis improves periodontal status in diabetic patients. Here, primary murine macrophages were exposed to CAPE. Target gene expression was assessed by whole-genome microarray, RT-PCR and Western blotting. The antioxidative and anti-inflammatory activities of CAPE were examined by exposure of the cells to hydrogen peroxide, saliva and periodontal pathogens. The involvement of HO1 was investigated with the HO1 inhibitor tin protoporphyrin(SnPP) and knockout mice for Nrf2, which is a transcription factor for detoxifying enzymes. CAPE increased HO1 and other heat shock proteins in murine macrophages. A p38 MAPK inhibitor and Nrf2 knockout attenuated CAPE-induced HO1 expression in macrophages. CAPE exerted strong antioxidative activity. Additionally, CAPE reduced the inflammatory response to saliva and periodontal pathogens. Blocking HO1 decreased the antioxidative activity and attenuated the anti-inflammatory activity of CAPE. In conclusion, CAPE exerted its antioxidative effects through the Nrf2-mediated HO1 pathway and its anti-inflammatory effects through NF-κB inhibition. However, preclinical models evaluating the use of CAPE in periodontal inflammation are necessary in future studies.

关键词： Caffeic acid phenethyl ester(CAPE) inflammatory response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The role of HBD-2,HBD-3,and calprotectin in the relationship between chronic periodontitis and atherosclerosis

引用

BIOCELL 2020年第3期44卷 337-344页

作者： MEHMET TASPINAR ALIHAN BOZOGLAN ABDULLAH SECKIN ERTUGRUL LEVENT ELMAS Faculty of Medicine Aksaray UniversityAksarayTurkey Faculty of Medicine Yuzuncu Yil UniversityVanTurkey department of periodontology Faculty of DentistryFırat UniversityElazığTurkey department of periodontology Faculty of DentistryYuzuncu Yil UniversityVanTurkey department of periodontology Faculty of Dentistryİzmir KatipÇelebi UniversityİzmirTurkey Faculty of Medicine Pamukkale UniversityDenizliTurkey

This study was carried out to compare individuals diagnosed with atherosclerosis and periodontal periodontitis based on the degree of change in the human beta-defensins(HBD)HBD-2,HBD-3,and *** is the most frequently observed cardiovascular *** and periodontal infections are known to provide a considerable basis for atheroma plaque *** study group consists of a total number of 40 subjects,with 20 patients diagnosed with atherosclerosis and chronic periodontitis and 20 systemically healthy patients diagnosed with chronic *** periodontal and blood parameters and HBD-2,HBD-3,and calprotectin biomarkers in the gingival crevicular fluid were *** both groups,following clinical periodontal treatment,a statistically significant decrease in white blood cells(WBC),low-density lipoproteins(LDL),fibrinogen,creatinine,and platelets(PLT),a statistically significant increase in high-density lipoproteins(HDL)in blood samples,statistically meaningful decrease in HBD-2,HBD-3,and calprotectin in the gingival crevicular fluid were *** values and HBD-2,HBD-3,calprotectin amounts in the gingival crevicular fluid were increased significantly in the test group compared to the control group.A positive correlation was observed between decreases in HBD-2,HBD-3,calprotectin,and clinical periodontal *** in systemic inflammation was observed after clinical periodontal *** is concluded that nonsurgical periodontal treatment of chronic periodontitis positively affects atherosclerosis prognosis.

关键词： Atherosclerosis Periodontal Diseases HBD-2 HBD-3 Calprotectin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Three-dimensional analysis of the physiological foramen geometry of maxillary and mandibular molars by means of micro-CT

引用

International Journal of Oral Science 2017年第3期9卷 151-157页

作者： Thomas Gerhard Wolf Frank Paqué Michael Sven Patyna Brita Willershausen Benjamín Briseno-Marroquín department of Operative dentistry University Medical Center of the Johannes Gutenberg University Mainz department of Endodontology Division of Preventive DentistryPeriodontologyand CariologyCenter of Dental MedicineUniversity of Zürich

The aim of this study was to investigate the physiological foramen diameter, shape and distance between physiological and anatomical apex of maxillary and mandibular first and second molars. Accurate knowledge of the physiological foramina morphology; thus, inherent mechanical shaping technical hindrances, is decisive when taking the corresponding root canal final preparation decision. The morphological dimensions of a total of 1 727 physiological foramina were investigated by means of micro-computed tomography. Mean narrow and wide （to a high number, oval） diameters of the physiological foramen were 0.24, 0.22 and 0.33 mm and 0.33, 0.31 and 0.42 mm in mesiobuccal （MB）, distobuccal （DB） and palatal （P） roots in maxillary first molars; 0.24, 0.22 and 0.33 mm and 0.41, 0.33 and 0.44 in MB, DB, and P roots in maxillary second molars. Mandibular first molars showed mean narrow and wide diameters of 0.24 and 0.30 mm and of 0.39 and 0.46 mm in mesial （M） and distal （D） roots; second mandibular molars showed 0.25 and 0.31 mm and 0.47 mm in M and D roots. The mean distance between the physiological foramina and anatomical apex was 0.82, 0.81 and 1.02 mm and 0.54, 0.43 and 0.63 mm in MB, DB and P roots of the maxillary first and second molars, respectively. A mean distance of 0.95 mm （M） and 1.05 mm （D） in the first and 0.78 mm （M） and 0.81 mm （D） in the second mandibular molars was observed. Based on the results obtained, assumable recommendations for final preparation size of the physiological foramen were calculated. However, when taking into consideration, the resulting standard deviations of marginal errors must be cautiously considered when taking a final decision in clinical endodontic treatment.

关键词： apical constriction final apical file maxillary and mandibular molars morphology micro-computed tomography physiological foramen

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Replacing Missing Teeth with Dental implants in Pubescent Patients—A Case Report

引用

Open Journal of Pediatrics 2015年第3期5卷 207-212页

作者： Wendy C. W. Wang Loana Tovar Suinaga Klenise S. Paranhos Sang-Choon Cho Ashman department of periodontology and implant dentistry New York University College of Dentistry New York USA

Tooth loss due to traumatic dental injury or congenital absence can cause functional and social- psychological consequences in youth. Pubescent children with missing teeth are often targets for school bullying. The treatment modality chosen can impact their well-being during their formative years. Despite the high success rate in adult patients, implant placement in young patients is not common due to its ankylosed nature and concerns with possible infra-occlusion in the future. However, skeletal growth and remodeling is a continuous process throughout life and postponement of dental implant placement does not necessary prevent future complication or need for replacement. Dental implant placement should be considered as a viable treatment option for pubescent patients if all other conventional alternatives fail to alleviate patients’ concerns both functionally and psychologically. This case report evaluates the considerations required to place dental implants in pubescent patients, as well as its advantages and disadvantages.

关键词： Dental implants Pubescent Social-Psychological Bullying

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exercise-induced irisin in bone and systemic irisin administration reveal new regulatory mechanisms of bone metabolism

引用

Bone Research 2017年第1期5卷 49-62页

作者： Jin Zhang Paloma Valverde Xiaofang Zhu Dana Murray Yuwei Wu Liming Yu Hua Jiang Michel M Dard Jin Huang Zhiwei Xu Qisheng Tu Jake Chen Division of Oral Biology Department of PeriodontologyTufts University School of Dental MedicineBostonMA 02111USA department of Anatomy Research Center for Integrative MedicineGuangzheu University of Chinese MedicineGuangzhouGuangdong Province 510405China department of Sciences Center for Sciences and Biomedical EngineeringBostonMA 02115USA department of periodontology and implant dentistry New York University College of DentistryNew YorkNY 10010USA department of Anatomy and Cell Biology Tufts University School of Medicine and Sackler School of Graduate Biomedical SciencesBostonMA 02111USA

Irisin is a polypeptide hormone derived from the proteolytic cleavage of fibronectin-type III domain- containing 5 （FNDC5） protein. Once released to circulation upon exercise or cold exposure, irisin stimulates browning of white adipose tissue （WAT） and uncoupling protein I （UCP1） expression, leading to an increase in total body energy expenditure by augmented UCPl-mediated thermogenesis. It is currently unknown whether irisin is secreted by bone upon exercise or whether it regulates bone metabolism in vivo. In this study, we found that 2 weeks of voluntary wheel-running exercise induced high levels of FNDC5 messenger RNA as well as FNDC5/irisin protein expression in murine bone tissues. Increased immunoreactivity due to exercise-induced FNDC5/irisin expression was detected in different regions of exercised femoral bones, including growth plate, trabecular bone, cortical bone, articular cartilage, and bone-tendon interface. Exercise also increased expression of osteogenic markers in bone and that of UCP1 in WAT, and led to bodyweight loss. Irisin intraperitoneal （IP） administration resulted in increased trabecular and cortical bone thickness and osteoblasts numbers, and concurrently induced UCP1 expression in subcutaneous WAT. Lentiviral FNDC5 IP administration increased cortical bone thickness. In vitro studies in bone cells revealed irisin increases osteoblastogenesis and mineralization, and inhibits receptor activator of nuclear factor-kB ligand （RANKL）- induced osteoclastogenesis. Taken together, our findings show that voluntary exercise increases irisin production in bone, and that an increase in circulating irisin levels enhances osteogenesis in mice.

关键词： Exercise-induced irisin in bone and systemic irisin administration reveal new regulatory mechanisms of bone metabolism bone

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metabolic memory:mechanisms and diseases

引用

Signal Transduction and Targeted Therapy 2024年第3期9卷 1086-1114页

作者： Hao Dong Yuezhang Sun Lulingxiao Nie Aimin Cui Pengfei Zhao Wai Keung Leung Qi Wang State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases West China Hospital of StomatologySichuan UniversityChengduChina department of Prosthodontics West China Hospital of StomatologySichuan UniversityChengduChina periodontology and implant dentistry Division Faculty of DentistryThe University of Hong KongHong KongChina

Metabolic diseases and their complications impose health and economic burdens *** from past experimental studies and clinical trials suggests our body may have the ability to remember the past metabolic environment,such as hyperglycemia or hyperlipidemia,thus leading to chronic inflammatory disorders and other diseases even after the elimination of these metabolic *** long-term effects of that aberrant metabolism on the body have been summarized as metabolic memory and are found to assume a crucial role in states of health and *** molecular mechanisms collectively participate in metabolic memory management,resulting in different cellular alterations as well as tissue and organ dysfunctions,culminating in disease progression and even affecting *** elucidation and expansion of the concept of metabolic memory provides more comprehensive insight into pathogenic mechanisms underlying metabolic diseases and complications and promises to be a new target in disease detection and ***,we retrace the history of relevant research on metabolic memory and summarize its salient *** provide a detailed discussion of the mechanisms by which metabolic memory may be involved in disease development at molecular,cellular,and organ levels,with emphasis on the impact of epigenetic ***,we present some of the pivotal findings arguing in favor of targeting metabolic memory to develop therapeutic strategies for metabolic diseases and provide the latest reflections on the consequences of metabolic memory as well as their implications for human health and diseases.

关键词： diseases metabolism alterations

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Free and liposome form of gallic acid improves calvarial bone wound healing in Wistar rats

引用

Asian Pacific Journal of Tropical Biomedicine 2020年第4期10卷 156-163页

作者： Ahmet Altan Hatice Balci Yuce Ozkan Karatas Mehmet Murat Taskan Fikret Gevrek Sefa Colak Nihat Akbulut department of Oral and Maxillofacial Surgery Faculty of DentistryGaziosmanpasa UniversityTokatTurkey department of periodontology Faculty of DentistryGaziosmanpasa UniversityTokatTurkey department of Histology and Embryology Faculty of MedicineGaziosmanpasa UniversityTokatTurkey

Objective:To evaluate the effect of free and liposome form of gallic acid on bone regeneration in critical defects in Wistar ***:Thirty-two female Wistar rats were divided into four study groups:group 1,negative control;group 2,positive control;group 3,gallic acid powder;group 4,gallic acid liposome.A critical-sized defect was created in all *** 2 to 4 had xenograft,autograft and membrane placement while negative control rats did not receive any *** defect area was sutured and rats were kept alive for 30 *** the end of the study,a bone specimen including the defect area was removed from *** specimens were evaluated under the stereomicroscope,then underwent histological *** cell counts,osteoblast,osteoclast counts,receptor activator of nuclear factorκ-B(RANKL),osteoprotegerin(OPG),runt-related transcription factor 2(Runx2),bone morphogenetic protein-2(BMP-2),and alkaline phosphatase were ***:The biggest unhealed defect area was observed in the negative control group and the smallest was observed in the gallic acid liposome *** were no differences between the positive control group *** gallic acid powder group and the gallic acid powder group *** gallic acid liposome *** severity of inflammation was the highest in the negative control group and the lowest in the gallic acid liposome group with significant differences between the *** groups had similar osteoblast counts while osteoclast counts were the highest in the positive control *** acid groups had a lower number of osteoclasts compared with the positive control ***2 and alkaline phosphatase levels were similar in the groups while OPG and BMP-2 levels exhibited a significant increase compared with the negative control group and the positive control *** was similar in the negative control group,the positive control group,and the gallic acid powder groups but decreased in the gallic acid liposome

关键词： Anti-inflammatory Antioxidants Bone regeneration Gallic acid

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：